Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3300 and Pf1N1B4_3301 are separated by 15 nucleotides Pf1N1B4_3301 and Pf1N1B4_3302 are separated by 305 nucleotides Pf1N1B4_3302 and Pf1N1B4_3303 are separated by 11 nucleotides
Pf1N1B4_3300 - Allophanate hydrolase (EC 3.5.1.54), at 3,685,614 to 3,687,392
_3300
Pf1N1B4_3301 - Urea carboxylase (EC 6.3.4.6), at 3,687,408 to 3,691,049
_3301
Pf1N1B4_3302 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,691,355 to 3,691,996
_3302
Pf1N1B4_3303 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,692,008 to 3,692,733
_3303
Position (kb)
3687
3688
3689
3690
3691
3692 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3686.449 kb on + strand, within Pf1N1B4_3300 at 3686.449 kb on + strand, within Pf1N1B4_3300 at 3686.476 kb on + strand, within Pf1N1B4_3300 at 3686.477 kb on - strand, within Pf1N1B4_3300 at 3686.477 kb on - strand, within Pf1N1B4_3300 at 3686.689 kb on + strand, within Pf1N1B4_3300 at 3686.689 kb on + strand, within Pf1N1B4_3300 at 3686.690 kb on - strand, within Pf1N1B4_3300 at 3686.690 kb on - strand, within Pf1N1B4_3300 at 3686.846 kb on + strand, within Pf1N1B4_3300 at 3687.109 kb on + strand, within Pf1N1B4_3300 at 3687.109 kb on + strand, within Pf1N1B4_3300 at 3687.278 kb on - strand at 3687.352 kb on + strand at 3688.042 kb on + strand, within Pf1N1B4_3301 at 3688.042 kb on + strand, within Pf1N1B4_3301 at 3688.042 kb on + strand, within Pf1N1B4_3301 at 3688.043 kb on - strand, within Pf1N1B4_3301 at 3688.043 kb on - strand, within Pf1N1B4_3301 at 3688.043 kb on - strand, within Pf1N1B4_3301 at 3688.043 kb on - strand, within Pf1N1B4_3301 at 3688.096 kb on + strand, within Pf1N1B4_3301 at 3688.096 kb on + strand, within Pf1N1B4_3301 at 3688.096 kb on + strand, within Pf1N1B4_3301 at 3688.096 kb on + strand, within Pf1N1B4_3301 at 3688.096 kb on + strand, within Pf1N1B4_3301 at 3688.097 kb on - strand, within Pf1N1B4_3301 at 3688.097 kb on - strand at 3688.097 kb on - strand, within Pf1N1B4_3301 at 3688.097 kb on - strand, within Pf1N1B4_3301 at 3688.097 kb on - strand, within Pf1N1B4_3301 at 3688.097 kb on - strand at 3688.156 kb on + strand, within Pf1N1B4_3301 at 3688.156 kb on + strand, within Pf1N1B4_3301 at 3688.157 kb on - strand, within Pf1N1B4_3301 at 3688.183 kb on + strand, within Pf1N1B4_3301 at 3688.183 kb on + strand, within Pf1N1B4_3301 at 3688.184 kb on - strand, within Pf1N1B4_3301 at 3688.287 kb on + strand, within Pf1N1B4_3301 at 3688.300 kb on + strand, within Pf1N1B4_3301 at 3688.300 kb on + strand, within Pf1N1B4_3301 at 3688.300 kb on + strand, within Pf1N1B4_3301 at 3688.300 kb on + strand, within Pf1N1B4_3301 at 3688.300 kb on + strand, within Pf1N1B4_3301 at 3688.300 kb on + strand, within Pf1N1B4_3301 at 3688.300 kb on + strand, within Pf1N1B4_3301 at 3688.301 kb on - strand, within Pf1N1B4_3301 at 3689.101 kb on + strand, within Pf1N1B4_3301 at 3689.101 kb on + strand, within Pf1N1B4_3301 at 3689.101 kb on + strand, within Pf1N1B4_3301 at 3689.101 kb on + strand, within Pf1N1B4_3301 at 3689.102 kb on - strand, within Pf1N1B4_3301 at 3689.117 kb on - strand, within Pf1N1B4_3301 at 3689.117 kb on - strand, within Pf1N1B4_3301 at 3689.140 kb on + strand, within Pf1N1B4_3301 at 3689.140 kb on + strand, within Pf1N1B4_3301 at 3689.141 kb on - strand, within Pf1N1B4_3301 at 3689.141 kb on - strand, within Pf1N1B4_3301 at 3689.231 kb on + strand, within Pf1N1B4_3301 at 3689.232 kb on - strand, within Pf1N1B4_3301 at 3689.238 kb on + strand at 3689.238 kb on + strand, within Pf1N1B4_3301 at 3689.238 kb on + strand, within Pf1N1B4_3301 at 3689.238 kb on + strand, within Pf1N1B4_3301 at 3689.238 kb on + strand, within Pf1N1B4_3301 at 3689.238 kb on + strand, within Pf1N1B4_3301 at 3689.239 kb on - strand, within Pf1N1B4_3301 at 3689.239 kb on - strand, within Pf1N1B4_3301 at 3689.239 kb on - strand, within Pf1N1B4_3301 at 3689.239 kb on - strand, within Pf1N1B4_3301 at 3689.239 kb on - strand, within Pf1N1B4_3301 at 3689.297 kb on + strand, within Pf1N1B4_3301 at 3689.298 kb on - strand, within Pf1N1B4_3301 at 3689.298 kb on - strand, within Pf1N1B4_3301 at 3689.298 kb on - strand, within Pf1N1B4_3301 at 3689.315 kb on - strand at 3689.350 kb on + strand, within Pf1N1B4_3301 at 3689.351 kb on - strand, within Pf1N1B4_3301 at 3689.351 kb on - strand, within Pf1N1B4_3301 at 3689.351 kb on - strand, within Pf1N1B4_3301 at 3689.645 kb on - strand, within Pf1N1B4_3301 at 3689.702 kb on - strand, within Pf1N1B4_3301 at 3689.702 kb on - strand, within Pf1N1B4_3301 at 3689.801 kb on - strand, within Pf1N1B4_3301 at 3689.897 kb on - strand, within Pf1N1B4_3301 at 3690.025 kb on + strand, within Pf1N1B4_3301 at 3690.025 kb on + strand, within Pf1N1B4_3301 at 3690.025 kb on + strand, within Pf1N1B4_3301 at 3690.025 kb on + strand, within Pf1N1B4_3301 at 3690.197 kb on - strand, within Pf1N1B4_3301 at 3690.197 kb on - strand at 3690.197 kb on - strand, within Pf1N1B4_3301 at 3690.197 kb on - strand, within Pf1N1B4_3301 at 3690.197 kb on - strand, within Pf1N1B4_3301 at 3690.448 kb on + strand, within Pf1N1B4_3301 at 3690.448 kb on + strand, within Pf1N1B4_3301 at 3690.448 kb on + strand, within Pf1N1B4_3301 at 3690.448 kb on + strand, within Pf1N1B4_3301 at 3690.448 kb on + strand, within Pf1N1B4_3301 at 3690.449 kb on - strand, within Pf1N1B4_3301 at 3690.449 kb on - strand, within Pf1N1B4_3301 at 3690.449 kb on - strand, within Pf1N1B4_3301 at 3690.449 kb on - strand, within Pf1N1B4_3301 at 3690.650 kb on + strand at 3690.871 kb on + strand at 3690.871 kb on + strand at 3690.872 kb on - strand at 3690.872 kb on - strand at 3690.872 kb on - strand at 3690.993 kb on - strand at 3691.284 kb on - strand at 3691.284 kb on - strand at 3691.284 kb on - strand at 3691.392 kb on + strand at 3691.392 kb on + strand at 3691.393 kb on - strand at 3691.470 kb on + strand at 3691.470 kb on + strand, within Pf1N1B4_3302 at 3691.471 kb on - strand, within Pf1N1B4_3302 at 3691.471 kb on - strand, within Pf1N1B4_3302 at 3691.471 kb on - strand, within Pf1N1B4_3302 at 3691.605 kb on + strand, within Pf1N1B4_3302 at 3691.605 kb on + strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.629 kb on + strand, within Pf1N1B4_3302 at 3691.749 kb on + strand, within Pf1N1B4_3302 at 3691.750 kb on - strand, within Pf1N1B4_3302 at 3691.773 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.813 kb on - strand, within Pf1N1B4_3302 at 3691.840 kb on - strand, within Pf1N1B4_3302
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 3,686,449 + Pf1N1B4_3300 0.47 +0.8 3,686,449 + Pf1N1B4_3300 0.47 +1.6 3,686,476 + Pf1N1B4_3300 0.48 +0.4 3,686,477 - Pf1N1B4_3300 0.49 +1.0 3,686,477 - Pf1N1B4_3300 0.49 -2.2 3,686,689 + Pf1N1B4_3300 0.60 -0.9 3,686,689 + Pf1N1B4_3300 0.60 -0.6 3,686,690 - Pf1N1B4_3300 0.60 -0.5 3,686,690 - Pf1N1B4_3300 0.60 -0.6 3,686,846 + Pf1N1B4_3300 0.69 +0.5 3,687,109 + Pf1N1B4_3300 0.84 -1.7 3,687,109 + Pf1N1B4_3300 0.84 -0.0 3,687,278 - -0.0 3,687,352 + -0.5 3,688,042 + Pf1N1B4_3301 0.17 +1.0 3,688,042 + Pf1N1B4_3301 0.17 -0.8 3,688,042 + Pf1N1B4_3301 0.17 +0.4 3,688,043 - Pf1N1B4_3301 0.17 -1.0 3,688,043 - Pf1N1B4_3301 0.17 -2.3 3,688,043 - Pf1N1B4_3301 0.17 +0.7 3,688,043 - Pf1N1B4_3301 0.17 +0.8 3,688,096 + Pf1N1B4_3301 0.19 -0.9 3,688,096 + Pf1N1B4_3301 0.19 -0.6 3,688,096 + Pf1N1B4_3301 0.19 +0.4 3,688,096 + Pf1N1B4_3301 0.19 +1.0 3,688,096 + Pf1N1B4_3301 0.19 +2.0 3,688,097 - Pf1N1B4_3301 0.19 -0.3 3,688,097 - +0.4 3,688,097 - Pf1N1B4_3301 0.19 +0.5 3,688,097 - Pf1N1B4_3301 0.19 +1.4 3,688,097 - Pf1N1B4_3301 0.19 -0.4 3,688,097 - -0.0 3,688,156 + Pf1N1B4_3301 0.21 +1.0 3,688,156 + Pf1N1B4_3301 0.21 -0.2 3,688,157 - Pf1N1B4_3301 0.21 -0.2 3,688,183 + Pf1N1B4_3301 0.21 -0.3 3,688,183 + Pf1N1B4_3301 0.21 -0.0 3,688,184 - Pf1N1B4_3301 0.21 -0.0 3,688,287 + Pf1N1B4_3301 0.24 -0.2 3,688,300 + Pf1N1B4_3301 0.24 -0.2 3,688,300 + Pf1N1B4_3301 0.24 +0.5 3,688,300 + Pf1N1B4_3301 0.24 -0.0 3,688,300 + Pf1N1B4_3301 0.24 +0.2 3,688,300 + Pf1N1B4_3301 0.24 +1.5 3,688,300 + Pf1N1B4_3301 0.24 +0.4 3,688,300 + Pf1N1B4_3301 0.24 -0.1 3,688,301 - Pf1N1B4_3301 0.25 +0.5 3,689,101 + Pf1N1B4_3301 0.46 -0.0 3,689,101 + Pf1N1B4_3301 0.46 +1.0 3,689,101 + Pf1N1B4_3301 0.46 -0.3 3,689,101 + Pf1N1B4_3301 0.46 +1.7 3,689,102 - Pf1N1B4_3301 0.47 -0.2 3,689,117 - Pf1N1B4_3301 0.47 -1.8 3,689,117 - Pf1N1B4_3301 0.47 +0.1 3,689,140 + Pf1N1B4_3301 0.48 -0.8 3,689,140 + Pf1N1B4_3301 0.48 +2.0 3,689,141 - Pf1N1B4_3301 0.48 -0.7 3,689,141 - Pf1N1B4_3301 0.48 +0.3 3,689,231 + Pf1N1B4_3301 0.50 +0.0 3,689,232 - Pf1N1B4_3301 0.50 +0.7 3,689,238 + -1.0 3,689,238 + Pf1N1B4_3301 0.50 -0.8 3,689,238 + Pf1N1B4_3301 0.50 -0.7 3,689,238 + Pf1N1B4_3301 0.50 +0.4 3,689,238 + Pf1N1B4_3301 0.50 -0.2 3,689,238 + Pf1N1B4_3301 0.50 -0.0 3,689,239 - Pf1N1B4_3301 0.50 -2.2 3,689,239 - Pf1N1B4_3301 0.50 +1.2 3,689,239 - Pf1N1B4_3301 0.50 -0.0 3,689,239 - Pf1N1B4_3301 0.50 -0.2 3,689,239 - Pf1N1B4_3301 0.50 +2.2 3,689,297 + Pf1N1B4_3301 0.52 -1.3 3,689,298 - Pf1N1B4_3301 0.52 -1.2 3,689,298 - Pf1N1B4_3301 0.52 -0.6 3,689,298 - Pf1N1B4_3301 0.52 +1.0 3,689,315 - +2.0 3,689,350 + Pf1N1B4_3301 0.53 -0.8 3,689,351 - Pf1N1B4_3301 0.53 +1.0 3,689,351 - Pf1N1B4_3301 0.53 -0.5 3,689,351 - Pf1N1B4_3301 0.53 -0.6 3,689,645 - Pf1N1B4_3301 0.61 +0.1 3,689,702 - Pf1N1B4_3301 0.63 -1.6 3,689,702 - Pf1N1B4_3301 0.63 +1.0 3,689,801 - Pf1N1B4_3301 0.66 -0.8 3,689,897 - Pf1N1B4_3301 0.68 +0.1 3,690,025 + Pf1N1B4_3301 0.72 -0.0 3,690,025 + Pf1N1B4_3301 0.72 +1.3 3,690,025 + Pf1N1B4_3301 0.72 +0.8 3,690,025 + Pf1N1B4_3301 0.72 +1.2 3,690,197 - Pf1N1B4_3301 0.77 -1.0 3,690,197 - -0.0 3,690,197 - Pf1N1B4_3301 0.77 -0.8 3,690,197 - Pf1N1B4_3301 0.77 +1.3 3,690,197 - Pf1N1B4_3301 0.77 +1.0 3,690,448 + Pf1N1B4_3301 0.83 +1.0 3,690,448 + Pf1N1B4_3301 0.83 +1.7 3,690,448 + Pf1N1B4_3301 0.83 +0.2 3,690,448 + Pf1N1B4_3301 0.83 -1.3 3,690,448 + Pf1N1B4_3301 0.83 +0.7 3,690,449 - Pf1N1B4_3301 0.83 -0.2 3,690,449 - Pf1N1B4_3301 0.83 +1.0 3,690,449 - Pf1N1B4_3301 0.83 -0.9 3,690,449 - Pf1N1B4_3301 0.83 +0.4 3,690,650 + -0.0 3,690,871 + -0.0 3,690,871 + +0.8 3,690,872 - +1.7 3,690,872 - +1.0 3,690,872 - -0.0 3,690,993 - +1.6 3,691,284 - -0.0 3,691,284 - +1.0 3,691,284 - -1.6 3,691,392 + -1.3 3,691,392 + +1.2 3,691,393 - +0.9 3,691,470 + +1.0 3,691,470 + Pf1N1B4_3302 0.18 +1.3 3,691,471 - Pf1N1B4_3302 0.18 +1.3 3,691,471 - Pf1N1B4_3302 0.18 +3.0 3,691,471 - Pf1N1B4_3302 0.18 -1.5 3,691,605 + Pf1N1B4_3302 0.39 -0.8 3,691,605 + Pf1N1B4_3302 0.39 -0.8 3,691,606 - Pf1N1B4_3302 0.39 -0.3 3,691,606 - Pf1N1B4_3302 0.39 -1.0 3,691,606 - Pf1N1B4_3302 0.39 +2.0 3,691,606 - Pf1N1B4_3302 0.39 -0.7 3,691,606 - -0.0 3,691,606 - Pf1N1B4_3302 0.39 -0.0 3,691,606 - Pf1N1B4_3302 0.39 +0.6 3,691,629 + Pf1N1B4_3302 0.43 -0.7 3,691,749 + Pf1N1B4_3302 0.61 -1.0 3,691,750 - Pf1N1B4_3302 0.62 -1.0 3,691,773 + Pf1N1B4_3302 0.65 -0.0 3,691,805 + Pf1N1B4_3302 0.70 +0.8 3,691,805 + Pf1N1B4_3302 0.70 -1.0 3,691,805 + -1.3 3,691,805 + Pf1N1B4_3302 0.70 +0.2 3,691,805 + Pf1N1B4_3302 0.70 +3.0 3,691,806 - Pf1N1B4_3302 0.70 +1.3 3,691,806 - Pf1N1B4_3302 0.70 -0.0 3,691,806 - Pf1N1B4_3302 0.70 -0.8 3,691,806 - Pf1N1B4_3302 0.70 +0.1 3,691,813 - Pf1N1B4_3302 0.71 -0.3 3,691,840 - Pf1N1B4_3302 0.76 -0.1
Or see this region's nucleotide sequence