Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2321 and Pf1N1B4_2322 overlap by 8 nucleotides Pf1N1B4_2322 and Pf1N1B4_2323 are separated by 155 nucleotides Pf1N1B4_2323 and Pf1N1B4_2324 are separated by 60 nucleotides
Pf1N1B4_2321 - FIG015287: Zinc protease, at 2,640,800 to 2,642,290
_2321
Pf1N1B4_2322 - FIG015547: peptidase, M16 family, at 2,642,283 to 2,643,638
_2322
Pf1N1B4_2323 - hypothetical protein, at 2,643,794 to 2,643,922
_2323
Pf1N1B4_2324 - Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1), at 2,643,983 to 2,645,479
_2324
Position (kb)
2642
2643
2644 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2641.657 kb on - strand, within Pf1N1B4_2321 at 2642.060 kb on + strand, within Pf1N1B4_2321 at 2642.215 kb on - strand at 2642.310 kb on + strand at 2642.311 kb on - strand at 2642.311 kb on - strand at 2642.345 kb on - strand at 2642.345 kb on - strand at 2642.486 kb on - strand, within Pf1N1B4_2322 at 2642.514 kb on + strand, within Pf1N1B4_2322 at 2642.669 kb on - strand, within Pf1N1B4_2322 at 2643.017 kb on - strand, within Pf1N1B4_2322 at 2643.137 kb on - strand, within Pf1N1B4_2322 at 2643.467 kb on - strand, within Pf1N1B4_2322 at 2643.675 kb on - strand at 2643.928 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 2,641,657 - Pf1N1B4_2321 0.57 +0.3 2,642,060 + Pf1N1B4_2321 0.85 +1.0 2,642,215 - +0.3 2,642,310 + +1.8 2,642,311 - +1.0 2,642,311 - +0.8 2,642,345 - +0.6 2,642,345 - +1.3 2,642,486 - Pf1N1B4_2322 0.15 -0.2 2,642,514 + Pf1N1B4_2322 0.17 -1.8 2,642,669 - Pf1N1B4_2322 0.28 -0.2 2,643,017 - Pf1N1B4_2322 0.54 +0.3 2,643,137 - Pf1N1B4_2322 0.63 +0.2 2,643,467 - Pf1N1B4_2322 0.87 -0.9 2,643,675 - -0.2 2,643,928 + -1.6
Or see this region's nucleotide sequence