Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1545 and Pf1N1B4_1546 are separated by 183 nucleotides Pf1N1B4_1546 and Pf1N1B4_1547 are separated by 13 nucleotides Pf1N1B4_1547 and Pf1N1B4_1548 are separated by 302 nucleotides
Pf1N1B4_1545 - NADP-dependent malic enzyme (EC 1.1.1.40), at 1,782,659 to 1,783,927
_1545
Pf1N1B4_1546 - Thermonuclease family protein, at 1,784,111 to 1,784,911
_1546
Pf1N1B4_1547 - LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc-dependent, at 1,784,925 to 1,785,155
_1547
Pf1N1B4_1548 - Helicase PriA essential for oriC/DnaA-independent DNA replication, at 1,785,458 to 1,787,251
_1548
Position (kb)
1784
1785 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1783.227 kb on + strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.546 kb on + strand, within Pf1N1B4_1545 at 1783.547 kb on - strand, within Pf1N1B4_1545 at 1783.547 kb on - strand, within Pf1N1B4_1545 at 1783.555 kb on + strand, within Pf1N1B4_1545 at 1783.555 kb on + strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.651 kb on + strand, within Pf1N1B4_1545 at 1783.651 kb on + strand, within Pf1N1B4_1545 at 1783.652 kb on - strand, within Pf1N1B4_1545 at 1783.652 kb on - strand, within Pf1N1B4_1545 at 1783.652 kb on - strand, within Pf1N1B4_1545 at 1783.661 kb on - strand, within Pf1N1B4_1545 at 1783.661 kb on - strand, within Pf1N1B4_1545 at 1783.752 kb on + strand, within Pf1N1B4_1545 at 1783.752 kb on + strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.758 kb on + strand, within Pf1N1B4_1545 at 1783.758 kb on + strand, within Pf1N1B4_1545 at 1783.791 kb on - strand, within Pf1N1B4_1545 at 1783.813 kb on + strand at 1783.815 kb on + strand at 1783.815 kb on + strand at 1783.815 kb on + strand at 1783.816 kb on - strand at 1783.816 kb on - strand at 1783.816 kb on - strand at 1783.816 kb on - strand at 1783.927 kb on + strand at 1783.927 kb on + strand at 1783.927 kb on + strand at 1783.927 kb on + strand at 1783.928 kb on - strand at 1783.928 kb on - strand at 1783.928 kb on - strand at 1783.928 kb on - strand at 1783.945 kb on + strand at 1783.946 kb on - strand at 1783.946 kb on - strand at 1783.973 kb on + strand at 1783.973 kb on + strand at 1783.974 kb on - strand at 1784.294 kb on + strand, within Pf1N1B4_1546 at 1784.294 kb on + strand, within Pf1N1B4_1546 at 1784.295 kb on - strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.719 kb on + strand, within Pf1N1B4_1546 at 1785.217 kb on - strand at 1785.217 kb on - strand at 1785.217 kb on - strand at 1785.217 kb on - strand at 1785.263 kb on + strand at 1785.375 kb on + strand at 1785.376 kb on - strand at 1785.376 kb on - strand at 1785.430 kb on + strand at 1785.430 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 1,783,227 + Pf1N1B4_1545 0.45 -0.2 1,783,228 - Pf1N1B4_1545 0.45 -2.2 1,783,228 - Pf1N1B4_1545 0.45 +0.5 1,783,228 - Pf1N1B4_1545 0.45 +0.4 1,783,281 + Pf1N1B4_1545 0.49 +0.1 1,783,281 + Pf1N1B4_1545 0.49 +1.1 1,783,281 + Pf1N1B4_1545 0.49 +0.0 1,783,281 + Pf1N1B4_1545 0.49 -0.2 1,783,281 + Pf1N1B4_1545 0.49 -0.1 1,783,281 + -0.2 1,783,281 + Pf1N1B4_1545 0.49 +0.6 1,783,281 + Pf1N1B4_1545 0.49 +1.8 1,783,282 - Pf1N1B4_1545 0.49 +0.4 1,783,282 - Pf1N1B4_1545 0.49 +1.0 1,783,282 - Pf1N1B4_1545 0.49 +0.9 1,783,282 - Pf1N1B4_1545 0.49 -1.3 1,783,282 - Pf1N1B4_1545 0.49 -0.6 1,783,282 - Pf1N1B4_1545 0.49 +0.9 1,783,282 - Pf1N1B4_1545 0.49 +0.4 1,783,282 - Pf1N1B4_1545 0.49 +0.6 1,783,282 - Pf1N1B4_1545 0.49 +0.7 1,783,282 - Pf1N1B4_1545 0.49 -1.2 1,783,282 - Pf1N1B4_1545 0.49 -0.2 1,783,282 - Pf1N1B4_1545 0.49 -0.5 1,783,282 - Pf1N1B4_1545 0.49 +0.4 1,783,282 - Pf1N1B4_1545 0.49 -0.2 1,783,282 - Pf1N1B4_1545 0.49 -0.3 1,783,282 - Pf1N1B4_1545 0.49 +0.0 1,783,546 + Pf1N1B4_1545 0.70 +0.0 1,783,547 - Pf1N1B4_1545 0.70 -1.5 1,783,547 - Pf1N1B4_1545 0.70 +1.0 1,783,555 + Pf1N1B4_1545 0.71 -0.1 1,783,555 + Pf1N1B4_1545 0.71 +0.8 1,783,556 - Pf1N1B4_1545 0.71 +0.2 1,783,556 - Pf1N1B4_1545 0.71 +0.8 1,783,556 - Pf1N1B4_1545 0.71 -0.5 1,783,556 - Pf1N1B4_1545 0.71 +1.4 1,783,651 + Pf1N1B4_1545 0.78 +1.4 1,783,651 + Pf1N1B4_1545 0.78 +0.7 1,783,652 - Pf1N1B4_1545 0.78 +1.4 1,783,652 - Pf1N1B4_1545 0.78 +0.1 1,783,652 - Pf1N1B4_1545 0.78 -0.0 1,783,661 - Pf1N1B4_1545 0.79 -0.5 1,783,661 - Pf1N1B4_1545 0.79 +0.5 1,783,752 + Pf1N1B4_1545 0.86 -0.5 1,783,752 + Pf1N1B4_1545 0.86 +1.4 1,783,753 - Pf1N1B4_1545 0.86 +1.4 1,783,753 - Pf1N1B4_1545 0.86 +0.8 1,783,753 - Pf1N1B4_1545 0.86 +2.4 1,783,753 - Pf1N1B4_1545 0.86 +1.1 1,783,753 - Pf1N1B4_1545 0.86 -0.1 1,783,753 - Pf1N1B4_1545 0.86 +2.9 1,783,753 - Pf1N1B4_1545 0.86 +0.5 1,783,753 - Pf1N1B4_1545 0.86 -0.1 1,783,753 - Pf1N1B4_1545 0.86 -0.2 1,783,758 + Pf1N1B4_1545 0.87 -0.2 1,783,758 + Pf1N1B4_1545 0.87 +0.7 1,783,791 - Pf1N1B4_1545 0.89 +1.0 1,783,813 + -0.1 1,783,815 + -0.2 1,783,815 + +2.4 1,783,815 + +0.2 1,783,816 - -0.5 1,783,816 - -0.5 1,783,816 - -0.4 1,783,816 - +0.7 1,783,927 + +0.6 1,783,927 + +0.6 1,783,927 + +1.7 1,783,927 + +0.5 1,783,928 - +1.0 1,783,928 - +0.7 1,783,928 - -0.2 1,783,928 - -0.5 1,783,945 + -0.6 1,783,946 - -0.6 1,783,946 - +1.4 1,783,973 + -0.2 1,783,973 + +0.1 1,783,974 - +0.3 1,784,294 + Pf1N1B4_1546 0.23 -1.5 1,784,294 + Pf1N1B4_1546 0.23 -1.2 1,784,295 - Pf1N1B4_1546 0.23 -0.4 1,784,562 + Pf1N1B4_1546 0.56 -2.1 1,784,562 + Pf1N1B4_1546 0.56 -1.0 1,784,562 + Pf1N1B4_1546 0.56 +0.5 1,784,562 + Pf1N1B4_1546 0.56 -1.0 1,784,562 + Pf1N1B4_1546 0.56 +0.4 1,784,562 + Pf1N1B4_1546 0.56 +0.4 1,784,562 + Pf1N1B4_1546 0.56 +0.8 1,784,563 - Pf1N1B4_1546 0.56 +1.4 1,784,563 - Pf1N1B4_1546 0.56 -0.6 1,784,563 - Pf1N1B4_1546 0.56 -0.7 1,784,563 - Pf1N1B4_1546 0.56 -0.1 1,784,563 - Pf1N1B4_1546 0.56 -0.6 1,784,563 - Pf1N1B4_1546 0.56 -1.0 1,784,719 + Pf1N1B4_1546 0.76 -1.5 1,785,217 - +0.4 1,785,217 - +0.0 1,785,217 - -0.6 1,785,217 - -0.2 1,785,263 + +1.4 1,785,375 + +1.2 1,785,376 - +0.8 1,785,376 - +1.0 1,785,430 + +0.7 1,785,430 + +1.4
Or see this region's nucleotide sequence