Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1076 and Pf1N1B4_1077 are separated by 7 nucleotides Pf1N1B4_1077 and Pf1N1B4_1078 are separated by 43 nucleotides Pf1N1B4_1078 and Pf1N1B4_1079 overlap by 1 nucleotides Pf1N1B4_1079 and Pf1N1B4_1080 are separated by 209 nucleotides
Pf1N1B4_1076 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45), at 1,209,601 to 1,210,125
_1076
Pf1N1B4_1077 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 1,210,133 to 1,210,705
_1077
Pf1N1B4_1078 - LptA, protein essential for LPS transport across the periplasm, at 1,210,749 to 1,211,240
_1078
Pf1N1B4_1079 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 1,211,240 to 1,211,965
_1079
Pf1N1B4_1080 - RNA polymerase sigma-54 factor RpoN, at 1,212,175 to 1,213,644
_1080
Position (kb)
1210
1211
1212 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1210.680 kb on + strand at 1211.964 kb on - strand at 1211.964 kb on - strand at 1211.964 kb on - strand at 1212.009 kb on - strand at 1212.027 kb on + strand at 1212.027 kb on + strand at 1212.028 kb on - strand at 1212.033 kb on + strand at 1212.033 kb on + strand at 1212.034 kb on - strand at 1212.044 kb on - strand at 1212.044 kb on - strand at 1212.044 kb on - strand at 1212.071 kb on - strand at 1212.071 kb on - strand at 1212.103 kb on + strand at 1212.131 kb on + strand at 1212.139 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 1,210,680 + -2.6 1,211,964 - -0.8 1,211,964 - -0.3 1,211,964 - +1.2 1,212,009 - +0.4 1,212,027 + -2.0 1,212,027 + +0.2 1,212,028 - -1.2 1,212,033 + -1.1 1,212,033 + +1.2 1,212,034 - -0.8 1,212,044 - +1.2 1,212,044 - +0.6 1,212,044 - -0.2 1,212,071 - -0.8 1,212,071 - -1.8 1,212,103 + -2.0 1,212,131 + +3.0 1,212,139 + +0.9
Or see this region's nucleotide sequence