Experiment: PYE with Carbenicillin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02418 and CCNA_R0057 are separated by 140 nucleotides CCNA_R0057 and CCNA_02419 are separated by 128 nucleotides CCNA_02419 and CCNA_02420 are separated by 10 nucleotides CCNA_02420 and CCNA_02421 are separated by 5 nucleotides
CCNA_02418: CCNA_02418 - uracil DNA glycosylase superfamily protein, at 2,561,765 to 2,563,204
_02418
CCNA_R0057: CCNA_R0057 - tRNA-His, at 2,563,345 to 2,563,421
_R0057
CCNA_02419: CCNA_02419 - TonB1 protein, at 2,563,550 to 2,564,281
_02419
CCNA_02420: CCNA_02420 - TonB accessory protein exbD, at 2,564,292 to 2,564,726
_02420
CCNA_02421: CCNA_02421 - TonB accessory protein exbB, at 2,564,732 to 2,565,640
_02421
Position (kb)
2563
2564
2565 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2562.591 kb on + strand, within CCNA_02418 at 2562.591 kb on - strand, within CCNA_02418 at 2562.615 kb on + strand, within CCNA_02418 at 2562.704 kb on + strand, within CCNA_02418 at 2562.704 kb on + strand, within CCNA_02418 at 2562.704 kb on + strand, within CCNA_02418 at 2562.704 kb on + strand, within CCNA_02418 at 2562.712 kb on - strand, within CCNA_02418 at 2562.735 kb on - strand, within CCNA_02418 at 2562.756 kb on + strand, within CCNA_02418 at 2562.766 kb on - strand, within CCNA_02418 at 2562.779 kb on - strand, within CCNA_02418 at 2562.794 kb on + strand, within CCNA_02418 at 2562.861 kb on - strand, within CCNA_02418 at 2562.894 kb on + strand, within CCNA_02418 at 2562.894 kb on + strand, within CCNA_02418 at 2562.895 kb on - strand, within CCNA_02418 at 2562.902 kb on - strand, within CCNA_02418 at 2562.902 kb on - strand, within CCNA_02418 at 2562.926 kb on - strand, within CCNA_02418 at 2562.928 kb on - strand, within CCNA_02418 at 2563.012 kb on + strand, within CCNA_02418 at 2563.012 kb on + strand, within CCNA_02418 at 2563.062 kb on + strand at 2563.062 kb on + strand at 2563.062 kb on + strand at 2563.116 kb on - strand at 2563.163 kb on - strand at 2563.206 kb on - strand at 2563.226 kb on - strand at 2563.235 kb on - strand at 2563.541 kb on + strand at 2563.601 kb on - strand at 2563.625 kb on - strand, within CCNA_02419 at 2563.637 kb on - strand, within CCNA_02419 at 2563.706 kb on + strand, within CCNA_02419 at 2563.714 kb on - strand, within CCNA_02419 at 2563.718 kb on + strand, within CCNA_02419 at 2563.726 kb on - strand, within CCNA_02419 at 2563.734 kb on + strand, within CCNA_02419 at 2563.780 kb on + strand, within CCNA_02419 at 2563.785 kb on + strand, within CCNA_02419 at 2563.793 kb on - strand, within CCNA_02419 at 2563.802 kb on + strand, within CCNA_02419 at 2563.810 kb on - strand, within CCNA_02419 at 2563.895 kb on - strand, within CCNA_02419 at 2564.081 kb on + strand, within CCNA_02419 at 2564.235 kb on - strand at 2564.303 kb on + strand at 2564.308 kb on + strand at 2564.668 kb on + strand, within CCNA_02420
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Carbenicillin 0.25 mg/ml remove 2,562,591 + CCNA_02418 0.57 +0.0 2,562,591 - CCNA_02418 0.57 -0.8 2,562,615 + CCNA_02418 0.59 +1.9 2,562,704 + CCNA_02418 0.65 -1.2 2,562,704 + CCNA_02418 0.65 -0.4 2,562,704 + CCNA_02418 0.65 +0.3 2,562,704 + CCNA_02418 0.65 +0.3 2,562,712 - CCNA_02418 0.66 +0.7 2,562,735 - CCNA_02418 0.67 -0.3 2,562,756 + CCNA_02418 0.69 -0.9 2,562,766 - CCNA_02418 0.70 +0.8 2,562,779 - CCNA_02418 0.70 +0.3 2,562,794 + CCNA_02418 0.71 +1.2 2,562,861 - CCNA_02418 0.76 -1.3 2,562,894 + CCNA_02418 0.78 -1.1 2,562,894 + CCNA_02418 0.78 +0.1 2,562,895 - CCNA_02418 0.78 +0.6 2,562,902 - CCNA_02418 0.79 +1.0 2,562,902 - CCNA_02418 0.79 -0.2 2,562,926 - CCNA_02418 0.81 +1.6 2,562,928 - CCNA_02418 0.81 +1.0 2,563,012 + CCNA_02418 0.87 -0.5 2,563,012 + CCNA_02418 0.87 +1.7 2,563,062 + +1.4 2,563,062 + -0.0 2,563,062 + +0.4 2,563,116 - +1.6 2,563,163 - -1.9 2,563,206 - -0.3 2,563,226 - +0.4 2,563,235 - -0.0 2,563,541 + +0.2 2,563,601 - +3.5 2,563,625 - CCNA_02419 0.10 +3.4 2,563,637 - CCNA_02419 0.12 +3.5 2,563,706 + CCNA_02419 0.21 +0.2 2,563,714 - CCNA_02419 0.22 +3.8 2,563,718 + CCNA_02419 0.23 +0.4 2,563,726 - CCNA_02419 0.24 +3.4 2,563,734 + CCNA_02419 0.25 -0.1 2,563,780 + CCNA_02419 0.31 +0.8 2,563,785 + CCNA_02419 0.32 +1.6 2,563,793 - CCNA_02419 0.33 +3.7 2,563,802 + CCNA_02419 0.34 +0.1 2,563,810 - CCNA_02419 0.36 +4.0 2,563,895 - CCNA_02419 0.47 +3.6 2,564,081 + CCNA_02419 0.73 -0.6 2,564,235 - +3.9 2,564,303 + +1.7 2,564,308 + -1.3 2,564,668 + CCNA_02420 0.86 +1.7
Or see this region's nucleotide sequence