Experiment: PYE with Carbenicillin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00674 and CCNA_00675 overlap by 35 nucleotides CCNA_00675 and CCNA_00676 overlap by 1 nucleotides CCNA_00676 and CCNA_00677 are separated by 147 nucleotides CCNA_00677 and CCNA_00678 are separated by 4 nucleotides
CCNA_00674: CCNA_00674 - NAD(FAD)-utilizing dehydrogenase, at 731,887 to 733,134
_00674
CCNA_00675: CCNA_00675 - VapC-like PIN domain ribonuclease, at 733,100 to 733,495
_00675
CCNA_00676: CCNA_00676 - PSK-family transcription factor, at 733,495 to 733,734
_00676
CCNA_00677: CCNA_00677 - LSU ribosomal protein L1P, at 733,882 to 734,571
_00677
CCNA_00678: CCNA_00678 - LSU ribosomal protein L11P, at 734,576 to 735,007
_00678
Position (kb)
733
734 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 732.512 kb on + strand, within CCNA_00674 at 732.536 kb on - strand, within CCNA_00674 at 732.574 kb on + strand, within CCNA_00674 at 732.580 kb on - strand, within CCNA_00674 at 732.586 kb on - strand, within CCNA_00674 at 732.611 kb on - strand, within CCNA_00674 at 732.642 kb on + strand, within CCNA_00674 at 732.656 kb on - strand, within CCNA_00674 at 732.676 kb on + strand, within CCNA_00674 at 732.685 kb on + strand, within CCNA_00674 at 732.693 kb on - strand, within CCNA_00674 at 732.693 kb on - strand, within CCNA_00674 at 732.721 kb on - strand, within CCNA_00674 at 732.723 kb on - strand, within CCNA_00674 at 732.756 kb on - strand, within CCNA_00674 at 732.799 kb on + strand, within CCNA_00674 at 732.808 kb on + strand, within CCNA_00674 at 732.808 kb on + strand, within CCNA_00674 at 732.840 kb on - strand, within CCNA_00674 at 732.841 kb on + strand, within CCNA_00674 at 732.841 kb on + strand, within CCNA_00674 at 732.843 kb on + strand, within CCNA_00674 at 732.849 kb on - strand, within CCNA_00674 at 732.921 kb on - strand, within CCNA_00674 at 732.936 kb on + strand, within CCNA_00674 at 732.936 kb on + strand, within CCNA_00674 at 732.936 kb on + strand, within CCNA_00674 at 732.944 kb on - strand, within CCNA_00674 at 732.944 kb on - strand, within CCNA_00674 at 732.944 kb on - strand, within CCNA_00674 at 732.944 kb on - strand, within CCNA_00674 at 732.987 kb on - strand, within CCNA_00674 at 733.016 kb on + strand at 733.016 kb on + strand at 733.024 kb on - strand at 733.024 kb on - strand at 733.024 kb on - strand at 733.092 kb on - strand at 733.106 kb on - strand at 733.129 kb on + strand at 733.138 kb on - strand at 733.140 kb on + strand, within CCNA_00675 at 733.145 kb on - strand, within CCNA_00675 at 733.170 kb on + strand, within CCNA_00675 at 733.197 kb on + strand, within CCNA_00675 at 733.197 kb on + strand, within CCNA_00675 at 733.202 kb on - strand, within CCNA_00675 at 733.214 kb on + strand, within CCNA_00675 at 733.222 kb on - strand, within CCNA_00675 at 733.282 kb on - strand, within CCNA_00675 at 733.288 kb on + strand, within CCNA_00675 at 733.296 kb on + strand, within CCNA_00675 at 733.296 kb on + strand, within CCNA_00675 at 733.304 kb on - strand, within CCNA_00675 at 733.309 kb on - strand, within CCNA_00675 at 733.309 kb on - strand, within CCNA_00675 at 733.309 kb on - strand, within CCNA_00675 at 733.309 kb on - strand, within CCNA_00675 at 733.309 kb on - strand, within CCNA_00675 at 733.340 kb on - strand, within CCNA_00675 at 733.366 kb on + strand, within CCNA_00675 at 733.444 kb on - strand, within CCNA_00675 at 733.451 kb on - strand, within CCNA_00675 at 733.488 kb on + strand at 733.491 kb on - strand at 733.534 kb on + strand, within CCNA_00676 at 733.542 kb on - strand, within CCNA_00676 at 733.607 kb on + strand, within CCNA_00676 at 733.607 kb on + strand at 733.607 kb on + strand, within CCNA_00676 at 733.607 kb on + strand, within CCNA_00676 at 733.607 kb on + strand, within CCNA_00676 at 733.615 kb on - strand, within CCNA_00676 at 733.637 kb on - strand, within CCNA_00676 at 733.652 kb on - strand, within CCNA_00676 at 733.757 kb on + strand at 733.787 kb on + strand at 733.792 kb on + strand at 733.825 kb on + strand at 733.828 kb on - strand at 733.855 kb on - strand at 733.863 kb on - strand at 733.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Carbenicillin 0.25 mg/ml remove 732,512 + CCNA_00674 0.50 -0.1 732,536 - CCNA_00674 0.52 +0.5 732,574 + CCNA_00674 0.55 -0.5 732,580 - CCNA_00674 0.56 +1.5 732,586 - CCNA_00674 0.56 -0.4 732,611 - CCNA_00674 0.58 +0.8 732,642 + CCNA_00674 0.60 -3.6 732,656 - CCNA_00674 0.62 +1.8 732,676 + CCNA_00674 0.63 +0.3 732,685 + CCNA_00674 0.64 -0.5 732,693 - CCNA_00674 0.65 +0.9 732,693 - CCNA_00674 0.65 +0.7 732,721 - CCNA_00674 0.67 +0.8 732,723 - CCNA_00674 0.67 +0.9 732,756 - CCNA_00674 0.70 +0.9 732,799 + CCNA_00674 0.73 -0.8 732,808 + CCNA_00674 0.74 -0.1 732,808 + CCNA_00674 0.74 -0.7 732,840 - CCNA_00674 0.76 +1.2 732,841 + CCNA_00674 0.76 -0.6 732,841 + CCNA_00674 0.76 -0.9 732,843 + CCNA_00674 0.77 -0.2 732,849 - CCNA_00674 0.77 +1.6 732,921 - CCNA_00674 0.83 +0.1 732,936 + CCNA_00674 0.84 -0.8 732,936 + CCNA_00674 0.84 -1.1 732,936 + CCNA_00674 0.84 +1.8 732,944 - CCNA_00674 0.85 +0.8 732,944 - CCNA_00674 0.85 +1.7 732,944 - CCNA_00674 0.85 +1.3 732,944 - CCNA_00674 0.85 +0.5 732,987 - CCNA_00674 0.88 +0.2 733,016 + -0.3 733,016 + +0.0 733,024 - +0.4 733,024 - +0.2 733,024 - +1.2 733,092 - +1.0 733,106 - +0.5 733,129 + +1.5 733,138 - +1.2 733,140 + CCNA_00675 0.10 -0.2 733,145 - CCNA_00675 0.11 +1.7 733,170 + CCNA_00675 0.18 -1.9 733,197 + CCNA_00675 0.24 +0.2 733,197 + CCNA_00675 0.24 -0.3 733,202 - CCNA_00675 0.26 +0.6 733,214 + CCNA_00675 0.29 +1.3 733,222 - CCNA_00675 0.31 +0.7 733,282 - CCNA_00675 0.46 +1.5 733,288 + CCNA_00675 0.47 +0.5 733,296 + CCNA_00675 0.49 -1.4 733,296 + CCNA_00675 0.49 -0.4 733,304 - CCNA_00675 0.52 +0.8 733,309 - CCNA_00675 0.53 +2.1 733,309 - CCNA_00675 0.53 -0.4 733,309 - CCNA_00675 0.53 +1.7 733,309 - CCNA_00675 0.53 +1.0 733,309 - CCNA_00675 0.53 +1.0 733,340 - CCNA_00675 0.61 +3.0 733,366 + CCNA_00675 0.67 +0.0 733,444 - CCNA_00675 0.87 +0.9 733,451 - CCNA_00675 0.89 +2.8 733,488 + -0.5 733,491 - +1.8 733,534 + CCNA_00676 0.16 +0.3 733,542 - CCNA_00676 0.20 +2.5 733,607 + CCNA_00676 0.47 +0.0 733,607 + +0.0 733,607 + CCNA_00676 0.47 -0.6 733,607 + CCNA_00676 0.47 +1.0 733,607 + CCNA_00676 0.47 -1.0 733,615 - CCNA_00676 0.50 +1.5 733,637 - CCNA_00676 0.59 +1.8 733,652 - CCNA_00676 0.65 +1.3 733,757 + -0.6 733,787 + -0.8 733,792 + -0.3 733,825 + +0.0 733,828 - +2.6 733,855 - +2.9 733,863 - +2.9 733,915 - +3.5
Or see this region's nucleotide sequence