Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00254

Experiment: PYE with Carbenicillin 0.25 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00253 and CCNA_00254 are separated by 136 nucleotidesCCNA_00254 and CCNA_00255 overlap by 4 nucleotides CCNA_00253: CCNA_00253 - aspartate-semialdehyde dehydrogenase, at 265,999 to 267,027 _00253 CCNA_00254: CCNA_00254 - RarD family membrane permease, at 267,164 to 268,066 _00254 CCNA_00255: CCNA_00255 - esterase, at 268,063 to 269,187 _00255 Position (kb) 267 268 269Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 266.173 kb on - strand, within CCNA_00253at 266.173 kb on - strand, within CCNA_00253at 266.190 kb on + strand, within CCNA_00253at 266.192 kb on - strand, within CCNA_00253at 266.208 kb on - strand, within CCNA_00253at 266.234 kb on - strand, within CCNA_00253at 266.240 kb on - strand, within CCNA_00253at 266.240 kb on - strand, within CCNA_00253at 266.240 kb on - strand, within CCNA_00253at 266.240 kb on - strand, within CCNA_00253at 266.240 kb on - strand, within CCNA_00253at 266.240 kb on - strand, within CCNA_00253at 266.279 kb on + strand, within CCNA_00253at 266.299 kb on + strand, within CCNA_00253at 266.312 kb on + strand, within CCNA_00253at 266.341 kb on + strand, within CCNA_00253at 266.364 kb on + strand, within CCNA_00253at 266.396 kb on + strand, within CCNA_00253at 266.427 kb on - strand, within CCNA_00253at 266.430 kb on + strand, within CCNA_00253at 266.430 kb on + strand, within CCNA_00253at 266.451 kb on + strand, within CCNA_00253at 266.458 kb on + strand, within CCNA_00253at 266.482 kb on + strand, within CCNA_00253at 266.482 kb on + strand, within CCNA_00253at 266.490 kb on + strand, within CCNA_00253at 266.505 kb on - strand, within CCNA_00253at 266.514 kb on + strand, within CCNA_00253at 266.514 kb on + strand, within CCNA_00253at 266.514 kb on + strand, within CCNA_00253at 266.522 kb on - strand, within CCNA_00253at 266.525 kb on + strand, within CCNA_00253at 266.573 kb on + strand, within CCNA_00253at 266.608 kb on + strand, within CCNA_00253at 266.627 kb on - strand, within CCNA_00253at 266.650 kb on + strand, within CCNA_00253at 266.706 kb on + strand, within CCNA_00253at 266.714 kb on - strand, within CCNA_00253at 266.728 kb on - strand, within CCNA_00253at 266.730 kb on + strand, within CCNA_00253at 266.764 kb on + strand, within CCNA_00253at 266.764 kb on + strand, within CCNA_00253at 266.772 kb on - strand, within CCNA_00253at 266.826 kb on - strand, within CCNA_00253at 266.830 kb on + strand, within CCNA_00253at 266.854 kb on - strand, within CCNA_00253at 266.892 kb on + strand, within CCNA_00253at 266.920 kb on - strand, within CCNA_00253at 266.943 kb on + strandat 266.943 kb on + strandat 266.951 kb on - strandat 266.968 kb on + strandat 267.006 kb on - strandat 267.009 kb on + strandat 267.009 kb on + strandat 267.016 kb on + strandat 267.016 kb on + strandat 267.017 kb on - strandat 267.017 kb on - strandat 267.021 kb on + strandat 267.024 kb on - strandat 267.024 kb on - strandat 267.061 kb on - strandat 267.154 kb on + strandat 267.154 kb on + strandat 267.155 kb on + strandat 267.162 kb on - strandat 267.190 kb on - strandat 267.205 kb on - strandat 267.249 kb on + strandat 267.257 kb on - strand, within CCNA_00254at 267.307 kb on + strand, within CCNA_00254at 267.333 kb on + strand, within CCNA_00254at 267.333 kb on + strand, within CCNA_00254at 267.333 kb on + strand, within CCNA_00254at 267.333 kb on + strand, within CCNA_00254at 267.341 kb on - strand, within CCNA_00254at 267.341 kb on - strand, within CCNA_00254at 267.341 kb on - strand, within CCNA_00254at 267.341 kb on - strand, within CCNA_00254at 267.341 kb on - strand, within CCNA_00254at 267.341 kb on - strand, within CCNA_00254at 267.341 kb on - strand, within CCNA_00254at 267.341 kb on - strand, within CCNA_00254at 267.345 kb on + strand, within CCNA_00254at 267.356 kb on - strand, within CCNA_00254at 267.363 kb on - strand, within CCNA_00254at 267.368 kb on + strand, within CCNA_00254at 267.374 kb on + strand, within CCNA_00254at 267.377 kb on - strand, within CCNA_00254at 267.393 kb on - strand, within CCNA_00254at 267.399 kb on - strand, within CCNA_00254at 267.443 kb on + strand, within CCNA_00254at 267.443 kb on + strand, within CCNA_00254at 267.443 kb on + strand, within CCNA_00254at 267.451 kb on - strand, within CCNA_00254at 267.451 kb on - strand, within CCNA_00254at 267.562 kb on + strand, within CCNA_00254at 267.570 kb on - strand, within CCNA_00254at 267.570 kb on - strand, within CCNA_00254at 267.645 kb on + strand, within CCNA_00254at 267.645 kb on + strand, within CCNA_00254at 267.650 kb on + strand, within CCNA_00254at 267.651 kb on - strand, within CCNA_00254at 267.653 kb on - strand, within CCNA_00254at 267.653 kb on - strand, within CCNA_00254at 267.653 kb on - strand, within CCNA_00254at 267.658 kb on - strand, within CCNA_00254at 267.658 kb on - strand, within CCNA_00254at 267.688 kb on + strand, within CCNA_00254at 267.688 kb on + strand, within CCNA_00254at 267.688 kb on + strand, within CCNA_00254at 267.688 kb on + strand, within CCNA_00254at 267.696 kb on - strand, within CCNA_00254at 267.708 kb on - strand, within CCNA_00254at 267.734 kb on - strand, within CCNA_00254at 267.746 kb on + strand, within CCNA_00254at 267.779 kb on + strandat 267.780 kb on + strand, within CCNA_00254at 267.843 kb on + strand, within CCNA_00254at 267.852 kb on + strand, within CCNA_00254at 267.860 kb on + strand, within CCNA_00254at 267.916 kb on - strand, within CCNA_00254at 267.937 kb on - strand, within CCNA_00254at 267.972 kb on - strand, within CCNA_00254at 268.010 kb on - strandat 268.059 kb on - strandat 268.062 kb on + strandat 268.069 kb on - strandat 268.072 kb on + strandat 268.281 kb on - strand, within CCNA_00255at 268.384 kb on - strand, within CCNA_00255at 268.615 kb on + strand, within CCNA_00255at 268.718 kb on - strand, within CCNA_00255at 268.765 kb on - strand, within CCNA_00255

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Carbenicillin 0.25 mg/ml
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266,173 - CCNA_00253 0.17 +0.8
266,173 - CCNA_00253 0.17 -1.4
266,190 + CCNA_00253 0.19 -1.2
266,192 - CCNA_00253 0.19 -1.6
266,208 - CCNA_00253 0.20 -1.5
266,234 - CCNA_00253 0.23 -2.2
266,240 - CCNA_00253 0.23 -0.3
266,240 - CCNA_00253 0.23 -2.1
266,240 - CCNA_00253 0.23 -0.3
266,240 - CCNA_00253 0.23 -0.9
266,240 - CCNA_00253 0.23 -0.3
266,240 - CCNA_00253 0.23 -1.0
266,279 + CCNA_00253 0.27 +3.1
266,299 + CCNA_00253 0.29 -1.2
266,312 + CCNA_00253 0.30 -0.9
266,341 + CCNA_00253 0.33 +0.3
266,364 + CCNA_00253 0.35 +2.3
266,396 + CCNA_00253 0.39 -1.4
266,427 - CCNA_00253 0.42 -0.2
266,430 + CCNA_00253 0.42 +0.5
266,430 + CCNA_00253 0.42 -0.4
266,451 + CCNA_00253 0.44 -0.5
266,458 + CCNA_00253 0.45 -1.0
266,482 + CCNA_00253 0.47 -0.2
266,482 + CCNA_00253 0.47 -0.1
266,490 + CCNA_00253 0.48 +0.4
266,505 - CCNA_00253 0.49 +0.6
266,514 + CCNA_00253 0.50 +0.8
266,514 + CCNA_00253 0.50 +0.8
266,514 + CCNA_00253 0.50 -1.0
266,522 - CCNA_00253 0.51 -1.6
266,525 + CCNA_00253 0.51 -1.6
266,573 + CCNA_00253 0.56 -1.1
266,608 + CCNA_00253 0.59 -0.7
266,627 - CCNA_00253 0.61 +1.1
266,650 + CCNA_00253 0.63 -0.3
266,706 + CCNA_00253 0.69 +0.2
266,714 - CCNA_00253 0.69 +0.1
266,728 - CCNA_00253 0.71 -0.8
266,730 + CCNA_00253 0.71 +0.3
266,764 + CCNA_00253 0.74 -1.0
266,764 + CCNA_00253 0.74 -0.1
266,772 - CCNA_00253 0.75 +1.1
266,826 - CCNA_00253 0.80 -1.0
266,830 + CCNA_00253 0.81 -0.7
266,854 - CCNA_00253 0.83 -2.3
266,892 + CCNA_00253 0.87 -0.4
266,920 - CCNA_00253 0.90 -0.4
266,943 + +0.0
266,943 + -0.7
266,951 - -0.9
266,968 + -1.9
267,006 - -0.7
267,009 + +0.2
267,009 + +1.1
267,016 + +0.5
267,016 + +1.3
267,017 - -1.7
267,017 - -0.7
267,021 + +1.5
267,024 - -0.5
267,024 - +1.1
267,061 - +0.5
267,154 + -0.1
267,154 + +0.1
267,155 + +1.0
267,162 - -1.1
267,190 - -1.2
267,205 - -1.7
267,249 + -0.1
267,257 - CCNA_00254 0.10 -1.2
267,307 + CCNA_00254 0.16 +0.1
267,333 + CCNA_00254 0.19 -0.2
267,333 + CCNA_00254 0.19 -1.4
267,333 + CCNA_00254 0.19 -0.8
267,333 + CCNA_00254 0.19 +1.7
267,341 - CCNA_00254 0.20 +0.1
267,341 - CCNA_00254 0.20 +1.0
267,341 - CCNA_00254 0.20 +1.2
267,341 - CCNA_00254 0.20 +0.3
267,341 - CCNA_00254 0.20 -1.4
267,341 - CCNA_00254 0.20 -0.9
267,341 - CCNA_00254 0.20 -1.0
267,341 - CCNA_00254 0.20 -0.5
267,345 + CCNA_00254 0.20 -0.3
267,356 - CCNA_00254 0.21 -1.1
267,363 - CCNA_00254 0.22 -1.4
267,368 + CCNA_00254 0.23 -2.4
267,374 + CCNA_00254 0.23 -3.1
267,377 - CCNA_00254 0.24 -1.2
267,393 - CCNA_00254 0.25 +1.4
267,399 - CCNA_00254 0.26 -1.0
267,443 + CCNA_00254 0.31 -1.4
267,443 + CCNA_00254 0.31 +0.8
267,443 + CCNA_00254 0.31 -0.3
267,451 - CCNA_00254 0.32 -2.1
267,451 - CCNA_00254 0.32 -0.4
267,562 + CCNA_00254 0.44 +0.4
267,570 - CCNA_00254 0.45 -1.1
267,570 - CCNA_00254 0.45 +0.4
267,645 + CCNA_00254 0.53 -0.1
267,645 + CCNA_00254 0.53 +0.4
267,650 + CCNA_00254 0.54 +0.0
267,651 - CCNA_00254 0.54 +1.2
267,653 - CCNA_00254 0.54 -0.5
267,653 - CCNA_00254 0.54 -2.3
267,653 - CCNA_00254 0.54 -1.5
267,658 - CCNA_00254 0.55 -1.4
267,658 - CCNA_00254 0.55 -1.2
267,688 + CCNA_00254 0.58 -0.1
267,688 + CCNA_00254 0.58 +0.5
267,688 + CCNA_00254 0.58 +1.6
267,688 + CCNA_00254 0.58 +0.4
267,696 - CCNA_00254 0.59 +1.0
267,708 - CCNA_00254 0.60 -2.1
267,734 - CCNA_00254 0.63 -0.2
267,746 + CCNA_00254 0.64 -0.1
267,779 + +2.8
267,780 + CCNA_00254 0.68 +0.2
267,843 + CCNA_00254 0.75 +0.7
267,852 + CCNA_00254 0.76 +0.2
267,860 + CCNA_00254 0.77 +0.5
267,916 - CCNA_00254 0.83 +0.2
267,937 - CCNA_00254 0.86 -1.4
267,972 - CCNA_00254 0.89 -0.1
268,010 - +0.8
268,059 - -0.3
268,062 + +0.8
268,069 - +1.3
268,072 + -0.7
268,281 - CCNA_00255 0.19 +1.3
268,384 - CCNA_00255 0.29 -0.3
268,615 + CCNA_00255 0.49 -0.9
268,718 - CCNA_00255 0.58 -0.2
268,765 - CCNA_00255 0.62 -0.4

Or see this region's nucleotide sequence