Experiment: Recovery broth with Carbenicillin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12030 and BBR_RS12035 are separated by 83 nucleotides BBR_RS12035 and BBR_RS12040 are separated by 47 nucleotides BBR_RS12040 and BBR_RS12045 are separated by 101 nucleotides
BBR_RS12030: BBR_RS12030 - F0F1 ATP synthase subunit epsilon, at 449,686 to 449,979
_RS12030
BBR_RS12035: BBR_RS12035 - endonuclease NucS, at 450,063 to 450,767
_RS12035
BBR_RS12040: BBR_RS12040 - peptidyl-prolyl cis-trans isomerase, at 450,815 to 451,768
_RS12040
BBR_RS12045: BBR_RS12045 - hypothetical protein, at 451,870 to 453,108
_RS12045
Position (kb) 450
451
452 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 449.942 kb on - strand, within BBR_RS12030 at 449.945 kb on + strand, within BBR_RS12030 at 449.945 kb on + strand, within BBR_RS12030 at 449.959 kb on - strand at 449.969 kb on + strand at 449.969 kb on - strand at 449.977 kb on - strand at 449.977 kb on - strand at 449.978 kb on + strand at 449.979 kb on - strand at 449.988 kb on + strand at 449.988 kb on + strand at 449.988 kb on + strand at 449.988 kb on + strand at 449.988 kb on + strand at 449.996 kb on - strand at 449.996 kb on - strand at 449.996 kb on - strand at 450.019 kb on + strand at 450.040 kb on + strand at 450.040 kb on + strand at 450.048 kb on + strand at 450.053 kb on + strand at 450.053 kb on + strand at 450.053 kb on + strand at 450.061 kb on - strand at 450.061 kb on - strand at 450.061 kb on - strand at 450.085 kb on + strand at 450.090 kb on + strand at 450.090 kb on + strand at 450.097 kb on + strand at 450.115 kb on - strand at 450.128 kb on + strand at 450.141 kb on - strand, within BBR_RS12035 at 450.190 kb on + strand, within BBR_RS12035 at 450.190 kb on + strand, within BBR_RS12035 at 450.190 kb on + strand, within BBR_RS12035 at 450.190 kb on + strand, within BBR_RS12035 at 450.190 kb on + strand, within BBR_RS12035 at 450.198 kb on - strand, within BBR_RS12035 at 450.198 kb on - strand, within BBR_RS12035 at 450.198 kb on - strand, within BBR_RS12035 at 450.198 kb on - strand, within BBR_RS12035 at 450.198 kb on - strand, within BBR_RS12035 at 450.198 kb on - strand, within BBR_RS12035 at 450.228 kb on + strand, within BBR_RS12035 at 450.228 kb on + strand, within BBR_RS12035 at 450.236 kb on - strand, within BBR_RS12035 at 450.236 kb on - strand, within BBR_RS12035 at 450.244 kb on - strand, within BBR_RS12035 at 450.250 kb on - strand, within BBR_RS12035 at 450.261 kb on + strand, within BBR_RS12035 at 450.264 kb on + strand, within BBR_RS12035 at 450.266 kb on + strand, within BBR_RS12035 at 450.266 kb on + strand, within BBR_RS12035 at 450.266 kb on + strand, within BBR_RS12035 at 450.286 kb on - strand, within BBR_RS12035 at 450.311 kb on + strand, within BBR_RS12035 at 450.311 kb on + strand, within BBR_RS12035 at 450.313 kb on + strand, within BBR_RS12035 at 450.320 kb on - strand, within BBR_RS12035 at 450.344 kb on + strand, within BBR_RS12035 at 450.344 kb on + strand, within BBR_RS12035 at 450.344 kb on + strand, within BBR_RS12035 at 450.349 kb on + strand, within BBR_RS12035 at 450.349 kb on + strand, within BBR_RS12035 at 450.352 kb on - strand, within BBR_RS12035 at 450.361 kb on + strand, within BBR_RS12035 at 450.379 kb on + strand, within BBR_RS12035 at 450.387 kb on - strand, within BBR_RS12035 at 450.389 kb on + strand, within BBR_RS12035 at 450.389 kb on + strand, within BBR_RS12035 at 450.397 kb on - strand, within BBR_RS12035 at 450.397 kb on - strand, within BBR_RS12035 at 450.402 kb on + strand, within BBR_RS12035 at 450.408 kb on - strand, within BBR_RS12035 at 450.408 kb on - strand, within BBR_RS12035 at 450.419 kb on + strand, within BBR_RS12035 at 450.437 kb on + strand, within BBR_RS12035 at 450.445 kb on - strand, within BBR_RS12035 at 450.470 kb on - strand, within BBR_RS12035 at 450.504 kb on - strand, within BBR_RS12035 at 450.514 kb on - strand, within BBR_RS12035 at 450.525 kb on - strand, within BBR_RS12035 at 450.536 kb on - strand, within BBR_RS12035 at 450.536 kb on - strand, within BBR_RS12035 at 450.582 kb on - strand, within BBR_RS12035 at 450.629 kb on - strand, within BBR_RS12035 at 450.641 kb on - strand, within BBR_RS12035 at 450.651 kb on + strand, within BBR_RS12035 at 450.656 kb on + strand, within BBR_RS12035 at 450.658 kb on + strand, within BBR_RS12035 at 450.658 kb on + strand, within BBR_RS12035 at 450.659 kb on - strand, within BBR_RS12035 at 450.659 kb on - strand, within BBR_RS12035 at 450.659 kb on - strand, within BBR_RS12035 at 450.659 kb on - strand, within BBR_RS12035 at 450.696 kb on + strand, within BBR_RS12035 at 450.696 kb on + strand, within BBR_RS12035 at 450.698 kb on - strand at 450.706 kb on + strand at 450.736 kb on - strand at 450.736 kb on - strand at 450.739 kb on - strand at 450.739 kb on - strand at 450.744 kb on + strand at 450.751 kb on + strand at 450.758 kb on - strand at 450.758 kb on - strand at 450.761 kb on + strand at 450.773 kb on + strand at 450.819 kb on - strand at 450.821 kb on - strand at 450.888 kb on + strand at 450.901 kb on - strand at 450.901 kb on - strand at 450.901 kb on - strand at 450.901 kb on - strand at 450.901 kb on - strand at 450.901 kb on - strand at 450.952 kb on - strand, within BBR_RS12040 at 450.963 kb on - strand, within BBR_RS12040 at 450.975 kb on - strand, within BBR_RS12040 at 450.975 kb on - strand, within BBR_RS12040 at 450.995 kb on - strand, within BBR_RS12040 at 451.001 kb on + strand, within BBR_RS12040 at 451.001 kb on + strand, within BBR_RS12040 at 451.016 kb on + strand, within BBR_RS12040 at 451.019 kb on - strand, within BBR_RS12040 at 451.019 kb on - strand, within BBR_RS12040 at 451.024 kb on + strand, within BBR_RS12040 at 451.026 kb on + strand, within BBR_RS12040 at 451.026 kb on + strand, within BBR_RS12040 at 451.057 kb on + strand, within BBR_RS12040 at 451.102 kb on - strand, within BBR_RS12040 at 451.113 kb on + strand, within BBR_RS12040 at 451.115 kb on + strand, within BBR_RS12040 at 451.121 kb on - strand, within BBR_RS12040 at 451.121 kb on - strand, within BBR_RS12040 at 451.121 kb on - strand, within BBR_RS12040 at 451.121 kb on - strand, within BBR_RS12040 at 451.158 kb on - strand, within BBR_RS12040 at 451.158 kb on - strand, within BBR_RS12040 at 451.158 kb on - strand, within BBR_RS12040 at 451.160 kb on + strand, within BBR_RS12040 at 451.160 kb on + strand, within BBR_RS12040 at 451.160 kb on + strand, within BBR_RS12040 at 451.176 kb on + strand, within BBR_RS12040 at 451.224 kb on + strand, within BBR_RS12040 at 451.224 kb on + strand, within BBR_RS12040 at 451.224 kb on + strand, within BBR_RS12040 at 451.224 kb on + strand, within BBR_RS12040 at 451.226 kb on + strand, within BBR_RS12040 at 451.226 kb on + strand, within BBR_RS12040 at 451.231 kb on - strand, within BBR_RS12040 at 451.231 kb on - strand, within BBR_RS12040 at 451.232 kb on - strand, within BBR_RS12040 at 451.232 kb on - strand, within BBR_RS12040 at 451.234 kb on + strand, within BBR_RS12040 at 451.234 kb on + strand, within BBR_RS12040 at 451.258 kb on + strand, within BBR_RS12040 at 451.265 kb on - strand, within BBR_RS12040 at 451.349 kb on - strand, within BBR_RS12040 at 451.364 kb on - strand, within BBR_RS12040 at 451.374 kb on - strand, within BBR_RS12040 at 451.377 kb on + strand, within BBR_RS12040 at 451.385 kb on - strand, within BBR_RS12040 at 451.455 kb on - strand, within BBR_RS12040 at 451.532 kb on - strand, within BBR_RS12040 at 451.662 kb on - strand, within BBR_RS12040 at 451.670 kb on - strand, within BBR_RS12040 at 451.748 kb on - strand at 451.750 kb on + strand at 451.770 kb on + strand at 451.770 kb on + strand at 451.778 kb on - strand at 451.821 kb on - strand at 451.843 kb on + strand at 451.853 kb on - strand at 451.896 kb on + strand at 451.961 kb on + strand at 451.971 kb on + strand at 452.010 kb on + strand, within BBR_RS12045 at 452.059 kb on + strand, within BBR_RS12045 at 452.071 kb on + strand, within BBR_RS12045
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Carbenicillin 0.001 mM remove 449,942 - BBR_RS12030 0.87 +0.4 449,945 + BBR_RS12030 0.88 -0.0 449,945 + BBR_RS12030 0.88 -0.3 449,959 - +1.4 449,969 + +1.5 449,969 - +0.1 449,977 - -0.4 449,977 - -0.3 449,978 + +0.9 449,979 - +0.3 449,988 + +0.5 449,988 + -0.1 449,988 + -1.4 449,988 + -0.5 449,988 + +1.7 449,996 - +0.1 449,996 - +0.0 449,996 - +0.6 450,019 + -0.8 450,040 + +0.3 450,040 + +1.5 450,048 + +0.3 450,053 + -0.8 450,053 + -0.5 450,053 + -0.1 450,061 - -0.0 450,061 - -0.4 450,061 - +1.2 450,085 + -0.6 450,090 + -0.5 450,090 + +0.3 450,097 + -0.2 450,115 - -0.6 450,128 + -0.2 450,141 - BBR_RS12035 0.11 -0.1 450,190 + BBR_RS12035 0.18 -0.1 450,190 + BBR_RS12035 0.18 -0.9 450,190 + BBR_RS12035 0.18 +0.6 450,190 + BBR_RS12035 0.18 -0.3 450,190 + BBR_RS12035 0.18 -0.3 450,198 - BBR_RS12035 0.19 +0.4 450,198 - BBR_RS12035 0.19 +2.1 450,198 - BBR_RS12035 0.19 -0.1 450,198 - BBR_RS12035 0.19 +0.7 450,198 - BBR_RS12035 0.19 +0.7 450,198 - BBR_RS12035 0.19 -0.5 450,228 + BBR_RS12035 0.23 +0.5 450,228 + BBR_RS12035 0.23 -0.4 450,236 - BBR_RS12035 0.25 -0.7 450,236 - BBR_RS12035 0.25 -0.5 450,244 - BBR_RS12035 0.26 -0.2 450,250 - BBR_RS12035 0.27 +0.5 450,261 + BBR_RS12035 0.28 -0.5 450,264 + BBR_RS12035 0.29 -0.7 450,266 + BBR_RS12035 0.29 +0.2 450,266 + BBR_RS12035 0.29 +0.0 450,266 + BBR_RS12035 0.29 -0.2 450,286 - BBR_RS12035 0.32 -0.9 450,311 + BBR_RS12035 0.35 +1.3 450,311 + BBR_RS12035 0.35 +1.0 450,313 + BBR_RS12035 0.35 -0.0 450,320 - BBR_RS12035 0.36 +1.2 450,344 + BBR_RS12035 0.40 +0.2 450,344 + BBR_RS12035 0.40 +0.9 450,344 + BBR_RS12035 0.40 -0.1 450,349 + BBR_RS12035 0.41 +0.0 450,349 + BBR_RS12035 0.41 +0.6 450,352 - BBR_RS12035 0.41 +1.7 450,361 + BBR_RS12035 0.42 -0.6 450,379 + BBR_RS12035 0.45 -0.1 450,387 - BBR_RS12035 0.46 +0.6 450,389 + BBR_RS12035 0.46 -0.2 450,389 + BBR_RS12035 0.46 +0.7 450,397 - BBR_RS12035 0.47 -0.8 450,397 - BBR_RS12035 0.47 -0.5 450,402 + BBR_RS12035 0.48 +0.2 450,408 - BBR_RS12035 0.49 -0.3 450,408 - BBR_RS12035 0.49 +1.0 450,419 + BBR_RS12035 0.50 -0.5 450,437 + BBR_RS12035 0.53 +0.0 450,445 - BBR_RS12035 0.54 +0.8 450,470 - BBR_RS12035 0.58 -0.3 450,504 - BBR_RS12035 0.63 +0.0 450,514 - BBR_RS12035 0.64 -0.1 450,525 - BBR_RS12035 0.66 +0.3 450,536 - BBR_RS12035 0.67 -0.1 450,536 - BBR_RS12035 0.67 +0.1 450,582 - BBR_RS12035 0.74 +0.5 450,629 - BBR_RS12035 0.80 +0.5 450,641 - BBR_RS12035 0.82 -0.8 450,651 + BBR_RS12035 0.83 +0.3 450,656 + BBR_RS12035 0.84 +0.3 450,658 + BBR_RS12035 0.84 -0.2 450,658 + BBR_RS12035 0.84 -0.5 450,659 - BBR_RS12035 0.85 +0.3 450,659 - BBR_RS12035 0.85 -0.1 450,659 - BBR_RS12035 0.85 +0.3 450,659 - BBR_RS12035 0.85 -0.9 450,696 + BBR_RS12035 0.90 +0.4 450,696 + BBR_RS12035 0.90 -0.4 450,698 - +0.9 450,706 + +0.6 450,736 - -0.2 450,736 - +0.3 450,739 - -0.6 450,739 - +0.3 450,744 + +0.5 450,751 + +1.8 450,758 - -0.3 450,758 - -0.2 450,761 + -0.3 450,773 + +0.6 450,819 - +0.6 450,821 - +0.6 450,888 + -0.8 450,901 - -1.2 450,901 - -0.5 450,901 - -2.1 450,901 - -2.1 450,901 - -2.2 450,901 - -1.5 450,952 - BBR_RS12040 0.14 -1.4 450,963 - BBR_RS12040 0.16 -1.8 450,975 - BBR_RS12040 0.17 -1.2 450,975 - BBR_RS12040 0.17 -0.8 450,995 - BBR_RS12040 0.19 -1.5 451,001 + BBR_RS12040 0.19 -1.2 451,001 + BBR_RS12040 0.19 -1.4 451,016 + BBR_RS12040 0.21 -1.6 451,019 - BBR_RS12040 0.21 -1.5 451,019 - BBR_RS12040 0.21 -3.2 451,024 + BBR_RS12040 0.22 -0.1 451,026 + BBR_RS12040 0.22 -0.3 451,026 + BBR_RS12040 0.22 -3.0 451,057 + BBR_RS12040 0.25 -2.5 451,102 - BBR_RS12040 0.30 -1.2 451,113 + BBR_RS12040 0.31 -1.3 451,115 + BBR_RS12040 0.31 -1.3 451,121 - BBR_RS12040 0.32 -1.7 451,121 - BBR_RS12040 0.32 -2.3 451,121 - BBR_RS12040 0.32 -3.3 451,121 - BBR_RS12040 0.32 -2.2 451,158 - BBR_RS12040 0.36 -0.8 451,158 - BBR_RS12040 0.36 -1.3 451,158 - BBR_RS12040 0.36 -0.9 451,160 + BBR_RS12040 0.36 -0.7 451,160 + BBR_RS12040 0.36 -2.3 451,160 + BBR_RS12040 0.36 -2.2 451,176 + BBR_RS12040 0.38 -1.0 451,224 + BBR_RS12040 0.43 -3.0 451,224 + BBR_RS12040 0.43 -1.7 451,224 + BBR_RS12040 0.43 -0.8 451,224 + BBR_RS12040 0.43 -2.3 451,226 + BBR_RS12040 0.43 -2.1 451,226 + BBR_RS12040 0.43 -1.3 451,231 - BBR_RS12040 0.44 -1.6 451,231 - BBR_RS12040 0.44 -0.9 451,232 - BBR_RS12040 0.44 -4.3 451,232 - BBR_RS12040 0.44 -3.3 451,234 + BBR_RS12040 0.44 +0.1 451,234 + BBR_RS12040 0.44 -0.4 451,258 + BBR_RS12040 0.46 -1.6 451,265 - BBR_RS12040 0.47 -2.5 451,349 - BBR_RS12040 0.56 +0.0 451,364 - BBR_RS12040 0.58 +2.3 451,374 - BBR_RS12040 0.59 -0.3 451,377 + BBR_RS12040 0.59 +1.3 451,385 - BBR_RS12040 0.60 +1.3 451,455 - BBR_RS12040 0.67 +0.7 451,532 - BBR_RS12040 0.75 -1.0 451,662 - BBR_RS12040 0.89 +2.3 451,670 - BBR_RS12040 0.90 +0.7 451,748 - -0.2 451,750 + +1.3 451,770 + -0.3 451,770 + -0.2 451,778 - +0.5 451,821 - -1.5 451,843 + +0.2 451,853 - -2.2 451,896 + +0.7 451,961 + +1.3 451,971 + +0.1 452,010 + BBR_RS12045 0.11 -2.2 452,059 + BBR_RS12045 0.15 -0.8 452,071 + BBR_RS12045 0.16 -2.4
Or see this region's nucleotide sequence