Experiment: Hexanoic 21 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0991 and Rru_A0992 are separated by 224 nucleotides Rru_A0992 and Rru_A0993 are separated by 310 nucleotides Rru_A0993 and Rru_A0994 are separated by 186 nucleotides
Rru_A0991: Rru_A0991 - Protein of unknown function UPF0126 (NCBI), at 1,179,289 to 1,180,005
_A0991
Rru_A0992: Rru_A0992 - hypothetical protein (NCBI), at 1,180,230 to 1,180,565
_A0992
Rru_A0993: Rru_A0993 - Transcriptional Regulator, NifA, Fis Family (NCBI), at 1,180,876 to 1,182,678
_A0993
Rru_A0994: Rru_A0994 - Nitrogenase cofactor biosynthesis protein NifB (NCBI), at 1,182,865 to 1,184,376
_A0994
Position (kb)
1180
1181
1182
1183 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1180.047 kb on + strand at 1180.047 kb on + strand at 1180.048 kb on - strand at 1180.048 kb on - strand at 1180.095 kb on + strand at 1180.095 kb on + strand at 1180.472 kb on + strand, within Rru_A0992 at 1180.472 kb on + strand, within Rru_A0992 at 1180.472 kb on + strand, within Rru_A0992 at 1180.472 kb on + strand, within Rru_A0992 at 1180.473 kb on - strand, within Rru_A0992 at 1180.473 kb on - strand, within Rru_A0992 at 1180.482 kb on - strand, within Rru_A0992 at 1180.563 kb on + strand at 1180.563 kb on + strand at 1180.563 kb on + strand at 1180.564 kb on - strand at 1180.564 kb on - strand at 1180.627 kb on + strand at 1180.627 kb on + strand at 1180.667 kb on - strand at 1180.694 kb on + strand at 1180.695 kb on - strand at 1180.695 kb on - strand at 1180.695 kb on - strand at 1180.960 kb on + strand at 1180.960 kb on + strand at 1180.960 kb on + strand at 1180.960 kb on + strand at 1180.961 kb on - strand at 1180.961 kb on - strand at 1180.961 kb on - strand at 1181.038 kb on + strand at 1181.038 kb on + strand at 1181.040 kb on + strand at 1181.040 kb on + strand at 1181.040 kb on + strand at 1181.041 kb on - strand at 1181.041 kb on - strand at 1181.177 kb on + strand, within Rru_A0993 at 1181.177 kb on + strand, within Rru_A0993 at 1181.666 kb on - strand, within Rru_A0993 at 1181.666 kb on - strand, within Rru_A0993 at 1181.666 kb on - strand, within Rru_A0993 at 1182.010 kb on + strand, within Rru_A0993 at 1182.011 kb on - strand, within Rru_A0993 at 1182.013 kb on + strand, within Rru_A0993 at 1182.168 kb on + strand, within Rru_A0993 at 1182.168 kb on + strand, within Rru_A0993 at 1182.169 kb on - strand, within Rru_A0993 at 1182.169 kb on - strand, within Rru_A0993 at 1182.169 kb on - strand, within Rru_A0993 at 1182.439 kb on + strand, within Rru_A0993 at 1182.439 kb on + strand, within Rru_A0993 at 1182.676 kb on + strand at 1182.731 kb on - strand at 1182.731 kb on - strand at 1182.731 kb on - strand at 1182.740 kb on + strand at 1182.748 kb on - strand at 1182.748 kb on - strand at 1182.879 kb on + strand at 1182.879 kb on + strand at 1182.879 kb on + strand at 1182.879 kb on + strand at 1182.879 kb on + strand at 1182.879 kb on + strand at 1182.879 kb on + strand at 1182.880 kb on - strand at 1182.880 kb on - strand at 1182.880 kb on - strand at 1182.880 kb on - strand at 1182.880 kb on - strand at 1182.880 kb on - strand at 1182.881 kb on + strand at 1182.881 kb on + strand at 1182.882 kb on - strand at 1182.882 kb on - strand at 1182.882 kb on - strand at 1182.914 kb on + strand at 1182.915 kb on - strand at 1183.051 kb on + strand, within Rru_A0994 at 1183.110 kb on + strand, within Rru_A0994 at 1183.110 kb on + strand, within Rru_A0994 at 1183.126 kb on + strand, within Rru_A0994 at 1183.141 kb on + strand, within Rru_A0994 at 1183.465 kb on + strand, within Rru_A0994 at 1183.465 kb on + strand, within Rru_A0994 at 1183.465 kb on + strand, within Rru_A0994 at 1183.466 kb on - strand, within Rru_A0994 at 1183.477 kb on + strand, within Rru_A0994 at 1183.478 kb on - strand, within Rru_A0994 at 1183.478 kb on - strand, within Rru_A0994 at 1183.478 kb on - strand, within Rru_A0994 at 1183.478 kb on - strand, within Rru_A0994 at 1183.478 kb on - strand, within Rru_A0994
Per-strain Table
Position Strand Gene LocusTag Fraction Hexanoic 21 mM; anaerobic remove 1,180,047 + -0.6 1,180,047 + +0.2 1,180,048 - -1.2 1,180,048 - -0.9 1,180,095 + +0.5 1,180,095 + -0.0 1,180,472 + Rru_A0992 0.72 -1.0 1,180,472 + Rru_A0992 0.72 -1.2 1,180,472 + Rru_A0992 0.72 +0.1 1,180,472 + Rru_A0992 0.72 +0.4 1,180,473 - Rru_A0992 0.72 -0.4 1,180,473 - Rru_A0992 0.72 -0.5 1,180,482 - Rru_A0992 0.75 -1.0 1,180,563 + -1.6 1,180,563 + +0.7 1,180,563 + -0.1 1,180,564 - -0.3 1,180,564 - -0.2 1,180,627 + -0.4 1,180,627 + +1.1 1,180,667 - -0.8 1,180,694 + +1.4 1,180,695 - -0.4 1,180,695 - +0.3 1,180,695 - +0.4 1,180,960 + +0.8 1,180,960 + -0.3 1,180,960 + +1.4 1,180,960 + +0.4 1,180,961 - -1.0 1,180,961 - -0.7 1,180,961 - -0.3 1,181,038 + -0.2 1,181,038 + -0.1 1,181,040 + -0.3 1,181,040 + -0.0 1,181,040 + +0.0 1,181,041 - -5.0 1,181,041 - +0.0 1,181,177 + Rru_A0993 0.17 -0.4 1,181,177 + Rru_A0993 0.17 +1.3 1,181,666 - Rru_A0993 0.44 -0.4 1,181,666 - Rru_A0993 0.44 -0.7 1,181,666 - Rru_A0993 0.44 -0.6 1,182,010 + Rru_A0993 0.63 +0.4 1,182,011 - Rru_A0993 0.63 +0.8 1,182,013 + Rru_A0993 0.63 +1.4 1,182,168 + Rru_A0993 0.72 +0.6 1,182,168 + Rru_A0993 0.72 -0.1 1,182,169 - Rru_A0993 0.72 +0.1 1,182,169 - Rru_A0993 0.72 -1.4 1,182,169 - Rru_A0993 0.72 +0.2 1,182,439 + Rru_A0993 0.87 +0.1 1,182,439 + Rru_A0993 0.87 +0.2 1,182,676 + -0.7 1,182,731 - -2.3 1,182,731 - +0.9 1,182,731 - -1.0 1,182,740 + +0.6 1,182,748 - +0.9 1,182,748 - -0.1 1,182,879 + +0.6 1,182,879 + -0.4 1,182,879 + -1.1 1,182,879 + +0.4 1,182,879 + -0.3 1,182,879 + -1.4 1,182,879 + +0.4 1,182,880 - +1.7 1,182,880 - +0.5 1,182,880 - -0.4 1,182,880 - +0.3 1,182,880 - +0.3 1,182,880 - +2.1 1,182,881 + -2.8 1,182,881 + -0.0 1,182,882 - +0.8 1,182,882 - -1.4 1,182,882 - -1.1 1,182,914 + +0.2 1,182,915 - -0.3 1,183,051 + Rru_A0994 0.12 +1.0 1,183,110 + Rru_A0994 0.16 +0.5 1,183,110 + Rru_A0994 0.16 +0.1 1,183,126 + Rru_A0994 0.17 -0.7 1,183,141 + Rru_A0994 0.18 +1.1 1,183,465 + Rru_A0994 0.40 +2.6 1,183,465 + Rru_A0994 0.40 -0.8 1,183,465 + Rru_A0994 0.40 +0.4 1,183,466 - Rru_A0994 0.40 -1.0 1,183,477 + Rru_A0994 0.40 -2.2 1,183,478 - Rru_A0994 0.41 +0.6 1,183,478 - Rru_A0994 0.41 -0.2 1,183,478 - Rru_A0994 0.41 -0.8 1,183,478 - Rru_A0994 0.41 -0.5 1,183,478 - Rru_A0994 0.41 -1.8
Or see this region's nucleotide sequence