Experiment: Hexanoic 21 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0227 and Rru_A0228 overlap by 4 nucleotides Rru_A0228 and Rru_A0229 are separated by 59 nucleotides Rru_A0229 and Rru_A0230 are separated by 105 nucleotides
Rru_A0227: Rru_A0227 - hypothetical protein (NCBI), at 279,747 to 280,085
_A0227
Rru_A0228: Rru_A0228 - Twin-arginine translocation pathway signal (NCBI), at 280,082 to 280,984
_A0228
Rru_A0229: Rru_A0229 - Glycosyl transferase, group 1 (NCBI), at 281,044 to 282,474
_A0229
Rru_A0230: Rru_A0230 - Asparagine synthase, glutamine-hydrolyzing (NCBI), at 282,580 to 284,556
_A0230
Position (kb) 281
282
283 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 280.171 kb on + strand at 280.171 kb on + strand at 280.171 kb on + strand at 280.171 kb on + strand at 280.171 kb on + strand at 280.171 kb on + strand at 280.172 kb on - strand at 280.172 kb on - strand at 280.172 kb on - strand at 280.172 kb on - strand at 280.172 kb on - strand at 280.172 kb on - strand at 280.172 kb on - strand at 280.173 kb on + strand, within Rru_A0228 at 280.173 kb on + strand, within Rru_A0228 at 280.173 kb on + strand, within Rru_A0228 at 280.173 kb on + strand, within Rru_A0228 at 280.174 kb on - strand, within Rru_A0228 at 280.174 kb on - strand, within Rru_A0228 at 280.174 kb on - strand, within Rru_A0228 at 280.174 kb on - strand, within Rru_A0228 at 280.487 kb on - strand, within Rru_A0228 at 280.644 kb on + strand, within Rru_A0228 at 280.645 kb on - strand, within Rru_A0228 at 280.805 kb on - strand, within Rru_A0228 at 280.984 kb on + strand at 280.984 kb on + strand at 281.190 kb on + strand, within Rru_A0229 at 281.190 kb on + strand, within Rru_A0229 at 281.191 kb on - strand, within Rru_A0229 at 281.291 kb on - strand, within Rru_A0229 at 281.408 kb on + strand, within Rru_A0229 at 281.447 kb on + strand, within Rru_A0229 at 281.447 kb on + strand, within Rru_A0229 at 281.447 kb on + strand, within Rru_A0229 at 281.448 kb on - strand, within Rru_A0229 at 281.448 kb on - strand, within Rru_A0229 at 281.448 kb on - strand, within Rru_A0229 at 281.567 kb on + strand, within Rru_A0229 at 281.885 kb on + strand, within Rru_A0229 at 281.885 kb on + strand, within Rru_A0229 at 281.886 kb on - strand, within Rru_A0229 at 281.886 kb on - strand, within Rru_A0229 at 281.886 kb on - strand, within Rru_A0229 at 281.886 kb on - strand, within Rru_A0229 at 282.201 kb on - strand, within Rru_A0229 at 282.274 kb on + strand, within Rru_A0229 at 282.274 kb on + strand, within Rru_A0229 at 282.275 kb on - strand, within Rru_A0229 at 282.284 kb on + strand, within Rru_A0229 at 282.285 kb on - strand, within Rru_A0229 at 282.307 kb on - strand, within Rru_A0229 at 282.346 kb on + strand at 282.347 kb on - strand at 282.347 kb on - strand at 282.347 kb on - strand at 282.439 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hexanoic 21 mM; anaerobic remove 280,171 + -0.9 280,171 + -1.9 280,171 + -0.0 280,171 + -1.4 280,171 + +0.8 280,171 + -1.3 280,172 - +0.0 280,172 - -0.8 280,172 - +0.2 280,172 - -1.0 280,172 - -0.0 280,172 - -0.1 280,172 - -2.1 280,173 + Rru_A0228 0.10 -0.8 280,173 + Rru_A0228 0.10 -2.4 280,173 + Rru_A0228 0.10 -1.5 280,173 + Rru_A0228 0.10 -0.4 280,174 - Rru_A0228 0.10 -1.0 280,174 - Rru_A0228 0.10 +0.0 280,174 - Rru_A0228 0.10 +0.4 280,174 - Rru_A0228 0.10 -1.4 280,487 - Rru_A0228 0.45 -0.1 280,644 + Rru_A0228 0.62 -3.3 280,645 - Rru_A0228 0.62 -2.2 280,805 - Rru_A0228 0.80 +1.0 280,984 + -0.5 280,984 + +0.0 281,190 + Rru_A0229 0.10 +0.5 281,190 + Rru_A0229 0.10 +0.4 281,191 - Rru_A0229 0.10 -2.2 281,291 - Rru_A0229 0.17 -0.2 281,408 + Rru_A0229 0.25 -0.9 281,447 + Rru_A0229 0.28 -0.5 281,447 + Rru_A0229 0.28 -3.9 281,447 + Rru_A0229 0.28 -0.9 281,448 - Rru_A0229 0.28 -5.0 281,448 - Rru_A0229 0.28 -0.1 281,448 - Rru_A0229 0.28 -1.9 281,567 + Rru_A0229 0.37 -0.2 281,885 + Rru_A0229 0.59 +0.1 281,885 + Rru_A0229 0.59 -2.7 281,886 - Rru_A0229 0.59 -2.4 281,886 - Rru_A0229 0.59 -1.3 281,886 - Rru_A0229 0.59 +0.8 281,886 - Rru_A0229 0.59 -0.1 282,201 - Rru_A0229 0.81 +0.7 282,274 + Rru_A0229 0.86 +0.6 282,274 + Rru_A0229 0.86 -1.6 282,275 - Rru_A0229 0.86 +0.1 282,284 + Rru_A0229 0.87 +0.5 282,285 - Rru_A0229 0.87 +1.7 282,307 - Rru_A0229 0.88 -1.1 282,346 + -0.9 282,347 - +0.6 282,347 - +1.4 282,347 - -3.0 282,439 + +2.4
Or see this region's nucleotide sequence