Experiment: Spectinomycin 0.0009765625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt masA and hisIE are separated by 29 nucleotides hisIE and Synpcc7942_1996 are separated by 55 nucleotides Synpcc7942_1996 and hliA are separated by 184 nucleotides hliA and Synpcc7942_1998 are separated by 185 nucleotides
Synpcc7942_1994: masA - 2,3-diketo-5-methylthio-1-phosphopentane phosphatase, at 2,064,180 to 2,064,896
masA
Synpcc7942_1995: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein, at 2,064,926 to 2,065,561
hisIE
Synpcc7942_1996: Synpcc7942_1996 - hypothetical protein, at 2,065,617 to 2,065,967
_1996
Synpcc7942_1997: hliA - high light-inducible protein, at 2,066,152 to 2,066,370
hliA
Synpcc7942_1998: Synpcc7942_1998 - GAF, at 2,066,556 to 2,067,143
_1998
Position (kb)
2065
2066 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2064.621 kb on - strand, within masA at 2064.655 kb on + strand at 2064.655 kb on + strand, within masA at 2064.658 kb on - strand, within masA at 2064.663 kb on - strand, within masA at 2064.670 kb on - strand, within masA at 2064.691 kb on + strand, within masA at 2064.742 kb on - strand at 2064.770 kb on + strand, within masA at 2064.791 kb on - strand, within masA at 2064.810 kb on + strand, within masA at 2064.826 kb on + strand at 2064.826 kb on + strand at 2064.826 kb on + strand at 2064.845 kb on - strand at 2064.871 kb on - strand at 2064.878 kb on - strand at 2064.918 kb on + strand at 2065.596 kb on - strand at 2065.597 kb on - strand at 2065.625 kb on + strand at 2065.625 kb on + strand at 2065.635 kb on - strand at 2065.679 kb on - strand, within Synpcc7942_1996 at 2065.686 kb on - strand, within Synpcc7942_1996 at 2065.704 kb on - strand, within Synpcc7942_1996 at 2065.717 kb on + strand, within Synpcc7942_1996 at 2065.754 kb on + strand, within Synpcc7942_1996 at 2065.756 kb on + strand, within Synpcc7942_1996 at 2065.763 kb on - strand, within Synpcc7942_1996 at 2065.789 kb on + strand, within Synpcc7942_1996 at 2065.831 kb on - strand, within Synpcc7942_1996 at 2065.831 kb on - strand, within Synpcc7942_1996 at 2065.844 kb on - strand, within Synpcc7942_1996 at 2065.893 kb on + strand, within Synpcc7942_1996 at 2065.902 kb on - strand, within Synpcc7942_1996 at 2065.941 kb on - strand at 2065.971 kb on + strand at 2065.981 kb on + strand at 2066.096 kb on + strand at 2066.113 kb on + strand at 2066.133 kb on + strand at 2066.133 kb on + strand at 2066.141 kb on - strand at 2066.149 kb on + strand at 2066.212 kb on + strand, within hliA at 2066.245 kb on + strand, within hliA at 2066.463 kb on + strand at 2066.532 kb on - strand at 2066.539 kb on - strand at 2066.539 kb on - strand at 2066.552 kb on + strand at 2066.552 kb on + strand at 2066.594 kb on + strand at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.630 kb on + strand, within Synpcc7942_1998 at 2066.633 kb on - strand, within Synpcc7942_1998 at 2066.633 kb on - strand, within Synpcc7942_1998 at 2066.635 kb on + strand, within Synpcc7942_1998 at 2066.641 kb on - strand, within Synpcc7942_1998 at 2066.641 kb on - strand, within Synpcc7942_1998 at 2066.643 kb on - strand, within Synpcc7942_1998 at 2066.657 kb on + strand, within Synpcc7942_1998 at 2066.681 kb on + strand, within Synpcc7942_1998 at 2066.689 kb on + strand at 2066.712 kb on - strand, within Synpcc7942_1998 at 2066.723 kb on - strand, within Synpcc7942_1998 at 2066.739 kb on - strand, within Synpcc7942_1998 at 2066.744 kb on - strand, within Synpcc7942_1998 at 2066.749 kb on - strand, within Synpcc7942_1998 at 2066.762 kb on - strand, within Synpcc7942_1998 at 2066.789 kb on + strand, within Synpcc7942_1998 at 2066.797 kb on - strand, within Synpcc7942_1998 at 2066.810 kb on + strand, within Synpcc7942_1998 at 2066.813 kb on - strand, within Synpcc7942_1998 at 2066.830 kb on + strand, within Synpcc7942_1998 at 2066.836 kb on - strand, within Synpcc7942_1998 at 2066.850 kb on + strand, within Synpcc7942_1998 at 2066.875 kb on + strand, within Synpcc7942_1998 at 2066.876 kb on + strand, within Synpcc7942_1998 at 2066.881 kb on + strand, within Synpcc7942_1998 at 2066.889 kb on - strand, within Synpcc7942_1998 at 2066.892 kb on + strand, within Synpcc7942_1998 at 2066.901 kb on + strand, within Synpcc7942_1998 at 2066.929 kb on - strand, within Synpcc7942_1998
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0009765625 mg/ml remove 2,064,621 - masA Synpcc7942_1994 0.62 +1.0 2,064,655 + +1.0 2,064,655 + masA Synpcc7942_1994 0.66 -0.8 2,064,658 - masA Synpcc7942_1994 0.67 +1.0 2,064,663 - masA Synpcc7942_1994 0.67 +1.5 2,064,670 - masA Synpcc7942_1994 0.68 -1.6 2,064,691 + masA Synpcc7942_1994 0.71 -1.4 2,064,742 - -0.0 2,064,770 + masA Synpcc7942_1994 0.82 -0.0 2,064,791 - masA Synpcc7942_1994 0.85 -1.0 2,064,810 + masA Synpcc7942_1994 0.88 -0.4 2,064,826 + -1.6 2,064,826 + -0.0 2,064,826 + -0.0 2,064,845 - +1.1 2,064,871 - +0.2 2,064,878 - -0.6 2,064,918 + -0.0 2,065,596 - -0.0 2,065,597 - -0.0 2,065,625 + +1.5 2,065,625 + -0.0 2,065,635 - -0.0 2,065,679 - Synpcc7942_1996 0.18 -3.2 2,065,686 - Synpcc7942_1996 0.20 -1.0 2,065,704 - Synpcc7942_1996 0.25 -2.5 2,065,717 + Synpcc7942_1996 0.28 -1.0 2,065,754 + Synpcc7942_1996 0.39 -2.4 2,065,756 + Synpcc7942_1996 0.40 -0.0 2,065,763 - Synpcc7942_1996 0.42 -1.6 2,065,789 + Synpcc7942_1996 0.49 -2.0 2,065,831 - Synpcc7942_1996 0.61 -2.6 2,065,831 - Synpcc7942_1996 0.61 -0.0 2,065,844 - Synpcc7942_1996 0.65 -1.0 2,065,893 + Synpcc7942_1996 0.79 -2.6 2,065,902 - Synpcc7942_1996 0.81 -0.0 2,065,941 - -3.2 2,065,971 + -0.0 2,065,981 + -0.0 2,066,096 + -1.0 2,066,113 + -0.8 2,066,133 + -0.0 2,066,133 + +0.5 2,066,141 - -0.9 2,066,149 + -1.6 2,066,212 + hliA Synpcc7942_1997 0.27 +1.0 2,066,245 + hliA Synpcc7942_1997 0.42 -0.0 2,066,463 + +1.9 2,066,532 - -0.5 2,066,539 - +4.5 2,066,539 - -1.6 2,066,552 + +3.1 2,066,552 + +1.0 2,066,594 + -0.8 2,066,625 + Synpcc7942_1998 0.12 -1.0 2,066,625 + Synpcc7942_1998 0.12 -1.4 2,066,625 + Synpcc7942_1998 0.12 +1.8 2,066,630 + Synpcc7942_1998 0.13 -0.0 2,066,633 - Synpcc7942_1998 0.13 -0.0 2,066,633 - Synpcc7942_1998 0.13 +1.3 2,066,635 + Synpcc7942_1998 0.13 -0.0 2,066,641 - Synpcc7942_1998 0.14 +2.0 2,066,641 - Synpcc7942_1998 0.14 +0.5 2,066,643 - Synpcc7942_1998 0.15 +1.5 2,066,657 + Synpcc7942_1998 0.17 +0.5 2,066,681 + Synpcc7942_1998 0.21 +0.5 2,066,689 + +3.1 2,066,712 - Synpcc7942_1998 0.27 -0.0 2,066,723 - Synpcc7942_1998 0.28 -0.5 2,066,739 - Synpcc7942_1998 0.31 +1.8 2,066,744 - Synpcc7942_1998 0.32 -0.9 2,066,749 - Synpcc7942_1998 0.33 +2.3 2,066,762 - Synpcc7942_1998 0.35 +1.3 2,066,789 + Synpcc7942_1998 0.40 -0.6 2,066,797 - Synpcc7942_1998 0.41 +2.3 2,066,810 + Synpcc7942_1998 0.43 -2.0 2,066,813 - Synpcc7942_1998 0.44 +2.1 2,066,830 + Synpcc7942_1998 0.47 +0.5 2,066,836 - Synpcc7942_1998 0.48 -0.0 2,066,850 + Synpcc7942_1998 0.50 +1.8 2,066,875 + Synpcc7942_1998 0.54 -2.6 2,066,876 + Synpcc7942_1998 0.54 +1.5 2,066,881 + Synpcc7942_1998 0.55 +1.5 2,066,889 - Synpcc7942_1998 0.57 -1.6 2,066,892 + Synpcc7942_1998 0.57 +2.3 2,066,901 + Synpcc7942_1998 0.59 -0.0 2,066,929 - Synpcc7942_1998 0.63 +0.4
Or see this region's nucleotide sequence