Experiment: Spectinomycin 0.0009765625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1321 and psaE are separated by 179 nucleotides psaE and fpg are separated by 108 nucleotides fpg and Synpcc7942_1324 are separated by 31 nucleotides
Synpcc7942_1321: Synpcc7942_1321 - hypothetical protein, at 1,350,414 to 1,351,433
_1321
Synpcc7942_1322: psaE - photosystem I reaction center subunit IV, at 1,351,613 to 1,351,840
psaE
Synpcc7942_1323: fpg - DNA-(apurinic or apyrimidinic site) lyase / Formamidopyrimidine-DNA glycosylase, at 1,351,949 to 1,352,797
fpg
Synpcc7942_1324: Synpcc7942_1324 - DEAD/DEAH box helicase-like, at 1,352,829 to 1,355,588
_1324
Position (kb)
1351
1352 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1350.619 kb on - strand, within Synpcc7942_1321 at 1350.624 kb on - strand, within Synpcc7942_1321 at 1350.634 kb on - strand, within Synpcc7942_1321 at 1350.643 kb on - strand, within Synpcc7942_1321 at 1350.653 kb on - strand, within Synpcc7942_1321 at 1350.666 kb on + strand, within Synpcc7942_1321 at 1350.674 kb on - strand, within Synpcc7942_1321 at 1350.718 kb on + strand, within Synpcc7942_1321 at 1350.733 kb on - strand, within Synpcc7942_1321 at 1350.741 kb on + strand, within Synpcc7942_1321 at 1350.752 kb on + strand, within Synpcc7942_1321 at 1350.752 kb on + strand at 1350.760 kb on - strand, within Synpcc7942_1321 at 1350.770 kb on - strand, within Synpcc7942_1321 at 1350.802 kb on + strand, within Synpcc7942_1321 at 1350.802 kb on - strand, within Synpcc7942_1321 at 1350.802 kb on - strand, within Synpcc7942_1321 at 1350.877 kb on + strand, within Synpcc7942_1321 at 1350.877 kb on + strand, within Synpcc7942_1321 at 1350.877 kb on + strand, within Synpcc7942_1321 at 1350.920 kb on - strand, within Synpcc7942_1321 at 1350.945 kb on - strand, within Synpcc7942_1321 at 1350.945 kb on - strand, within Synpcc7942_1321 at 1350.977 kb on - strand, within Synpcc7942_1321 at 1351.043 kb on + strand, within Synpcc7942_1321 at 1351.049 kb on + strand, within Synpcc7942_1321 at 1351.068 kb on - strand, within Synpcc7942_1321 at 1351.095 kb on + strand, within Synpcc7942_1321 at 1351.103 kb on + strand, within Synpcc7942_1321 at 1351.122 kb on + strand, within Synpcc7942_1321 at 1351.149 kb on - strand, within Synpcc7942_1321 at 1351.166 kb on + strand, within Synpcc7942_1321 at 1351.166 kb on + strand, within Synpcc7942_1321 at 1351.174 kb on - strand, within Synpcc7942_1321 at 1351.176 kb on - strand, within Synpcc7942_1321 at 1351.199 kb on + strand, within Synpcc7942_1321 at 1351.207 kb on - strand, within Synpcc7942_1321 at 1351.207 kb on - strand, within Synpcc7942_1321 at 1351.207 kb on - strand, within Synpcc7942_1321 at 1351.207 kb on - strand, within Synpcc7942_1321 at 1351.207 kb on - strand, within Synpcc7942_1321 at 1351.207 kb on - strand, within Synpcc7942_1321 at 1351.207 kb on - strand, within Synpcc7942_1321 at 1351.207 kb on - strand, within Synpcc7942_1321 at 1351.224 kb on + strand, within Synpcc7942_1321 at 1351.226 kb on - strand, within Synpcc7942_1321 at 1351.234 kb on + strand, within Synpcc7942_1321 at 1351.254 kb on + strand, within Synpcc7942_1321 at 1351.271 kb on + strand, within Synpcc7942_1321 at 1351.272 kb on - strand, within Synpcc7942_1321 at 1351.275 kb on - strand, within Synpcc7942_1321 at 1351.279 kb on - strand, within Synpcc7942_1321 at 1351.321 kb on + strand, within Synpcc7942_1321 at 1351.321 kb on + strand, within Synpcc7942_1321 at 1351.321 kb on + strand, within Synpcc7942_1321 at 1351.329 kb on - strand, within Synpcc7942_1321 at 1351.329 kb on - strand, within Synpcc7942_1321 at 1351.357 kb on + strand at 1351.362 kb on - strand at 1351.375 kb on - strand at 1351.378 kb on - strand at 1351.389 kb on + strand at 1351.397 kb on - strand at 1351.423 kb on - strand at 1351.429 kb on + strand at 1351.429 kb on + strand at 1351.429 kb on + strand at 1351.432 kb on - strand at 1351.432 kb on - strand at 1351.437 kb on - strand at 1351.438 kb on - strand at 1351.450 kb on + strand at 1351.470 kb on - strand at 1351.472 kb on + strand at 1351.472 kb on + strand at 1351.480 kb on - strand at 1351.480 kb on - strand at 1351.480 kb on - strand at 1351.537 kb on - strand at 1351.592 kb on - strand at 1351.594 kb on + strand at 1351.610 kb on + strand at 1351.618 kb on - strand at 1351.637 kb on - strand, within psaE at 1351.637 kb on - strand, within psaE at 1351.687 kb on + strand, within psaE at 1351.724 kb on + strand, within psaE at 1351.740 kb on + strand, within psaE at 1351.830 kb on + strand at 1351.939 kb on + strand at 1351.950 kb on + strand at 1351.950 kb on + strand at 1351.958 kb on - strand at 1351.974 kb on + strand at 1351.980 kb on + strand at 1351.992 kb on - strand at 1352.002 kb on + strand at 1352.011 kb on + strand at 1352.030 kb on + strand at 1352.030 kb on + strand at 1352.030 kb on + strand at 1352.056 kb on + strand, within fpg at 1352.057 kb on + strand, within fpg at 1352.061 kb on + strand, within fpg at 1352.061 kb on + strand, within fpg at 1352.061 kb on + strand, within fpg at 1352.066 kb on + strand, within fpg at 1352.071 kb on + strand, within fpg at 1352.071 kb on - strand, within fpg at 1352.077 kb on - strand, within fpg at 1352.138 kb on + strand, within fpg at 1352.143 kb on + strand, within fpg at 1352.146 kb on - strand, within fpg at 1352.146 kb on - strand, within fpg at 1352.167 kb on + strand, within fpg at 1352.167 kb on + strand, within fpg at 1352.169 kb on + strand, within fpg at 1352.208 kb on + strand, within fpg at 1352.211 kb on - strand, within fpg at 1352.265 kb on - strand, within fpg at 1352.265 kb on - strand at 1352.308 kb on + strand, within fpg at 1352.313 kb on + strand, within fpg at 1352.316 kb on - strand, within fpg at 1352.321 kb on - strand, within fpg at 1352.378 kb on - strand, within fpg at 1352.387 kb on - strand, within fpg at 1352.438 kb on - strand, within fpg at 1352.472 kb on - strand, within fpg at 1352.520 kb on + strand, within fpg at 1352.528 kb on - strand, within fpg at 1352.535 kb on + strand, within fpg at 1352.535 kb on + strand, within fpg at 1352.535 kb on + strand, within fpg at 1352.543 kb on - strand, within fpg at 1352.546 kb on + strand, within fpg at 1352.586 kb on + strand, within fpg at 1352.607 kb on + strand, within fpg at 1352.615 kb on - strand, within fpg at 1352.662 kb on - strand, within fpg at 1352.687 kb on + strand, within fpg at 1352.687 kb on + strand, within fpg at 1352.689 kb on - strand, within fpg at 1352.695 kb on - strand, within fpg at 1352.702 kb on + strand at 1352.718 kb on + strand at 1352.767 kb on + strand at 1352.767 kb on + strand at 1352.775 kb on - strand at 1352.798 kb on - strand at 1352.798 kb on - strand at 1352.798 kb on - strand at 1352.800 kb on - strand at 1352.802 kb on + strand at 1352.825 kb on - strand at 1352.825 kb on - strand at 1352.825 kb on - strand at 1352.827 kb on + strand at 1352.827 kb on + strand at 1352.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0009765625 mg/ml remove 1,350,619 - Synpcc7942_1321 0.20 -1.1 1,350,624 - Synpcc7942_1321 0.21 -0.1 1,350,634 - Synpcc7942_1321 0.22 -0.7 1,350,643 - Synpcc7942_1321 0.22 -1.0 1,350,653 - Synpcc7942_1321 0.23 -0.9 1,350,666 + Synpcc7942_1321 0.25 +0.9 1,350,674 - Synpcc7942_1321 0.25 +0.4 1,350,718 + Synpcc7942_1321 0.30 -1.1 1,350,733 - Synpcc7942_1321 0.31 -2.3 1,350,741 + Synpcc7942_1321 0.32 -0.6 1,350,752 + Synpcc7942_1321 0.33 -0.1 1,350,752 + -0.7 1,350,760 - Synpcc7942_1321 0.34 +0.9 1,350,770 - Synpcc7942_1321 0.35 -1.1 1,350,802 + Synpcc7942_1321 0.38 -0.6 1,350,802 - Synpcc7942_1321 0.38 +2.2 1,350,802 - Synpcc7942_1321 0.38 -1.5 1,350,877 + Synpcc7942_1321 0.45 -1.7 1,350,877 + Synpcc7942_1321 0.45 -2.7 1,350,877 + Synpcc7942_1321 0.45 -1.5 1,350,920 - Synpcc7942_1321 0.50 -1.1 1,350,945 - Synpcc7942_1321 0.52 -1.1 1,350,945 - Synpcc7942_1321 0.52 +1.1 1,350,977 - Synpcc7942_1321 0.55 -0.1 1,351,043 + Synpcc7942_1321 0.62 +0.7 1,351,049 + Synpcc7942_1321 0.62 +0.4 1,351,068 - Synpcc7942_1321 0.64 -0.4 1,351,095 + Synpcc7942_1321 0.67 -1.7 1,351,103 + Synpcc7942_1321 0.68 +1.1 1,351,122 + Synpcc7942_1321 0.69 -1.1 1,351,149 - Synpcc7942_1321 0.72 +2.4 1,351,166 + Synpcc7942_1321 0.74 -0.1 1,351,166 + Synpcc7942_1321 0.74 +1.9 1,351,174 - Synpcc7942_1321 0.75 -0.1 1,351,176 - Synpcc7942_1321 0.75 +1.0 1,351,199 + Synpcc7942_1321 0.77 +0.9 1,351,207 - Synpcc7942_1321 0.78 -0.1 1,351,207 - Synpcc7942_1321 0.78 -1.1 1,351,207 - Synpcc7942_1321 0.78 +1.4 1,351,207 - Synpcc7942_1321 0.78 +1.2 1,351,207 - Synpcc7942_1321 0.78 -0.1 1,351,207 - Synpcc7942_1321 0.78 -0.7 1,351,207 - Synpcc7942_1321 0.78 -0.6 1,351,207 - Synpcc7942_1321 0.78 -0.1 1,351,224 + Synpcc7942_1321 0.79 -0.1 1,351,226 - Synpcc7942_1321 0.80 -2.1 1,351,234 + Synpcc7942_1321 0.80 +0.9 1,351,254 + Synpcc7942_1321 0.82 +1.9 1,351,271 + Synpcc7942_1321 0.84 +1.4 1,351,272 - Synpcc7942_1321 0.84 -1.7 1,351,275 - Synpcc7942_1321 0.84 +1.4 1,351,279 - Synpcc7942_1321 0.85 +0.1 1,351,321 + Synpcc7942_1321 0.89 +2.0 1,351,321 + Synpcc7942_1321 0.89 -0.1 1,351,321 + Synpcc7942_1321 0.89 -1.7 1,351,329 - Synpcc7942_1321 0.90 -1.1 1,351,329 - Synpcc7942_1321 0.90 +0.9 1,351,357 + +0.9 1,351,362 - +1.7 1,351,375 - +0.9 1,351,378 - +0.1 1,351,389 + +0.9 1,351,397 - -0.5 1,351,423 - +1.3 1,351,429 + -0.1 1,351,429 + -0.3 1,351,429 + +1.2 1,351,432 - +0.9 1,351,432 - -1.7 1,351,437 - +1.3 1,351,438 - +1.2 1,351,450 + +0.9 1,351,470 - +1.9 1,351,472 + -0.1 1,351,472 + -1.1 1,351,480 - +1.4 1,351,480 - -1.7 1,351,480 - +2.3 1,351,537 - -0.7 1,351,592 - -1.1 1,351,594 + -0.1 1,351,610 + -1.7 1,351,618 - -0.1 1,351,637 - psaE Synpcc7942_1322 0.11 -2.1 1,351,637 - psaE Synpcc7942_1322 0.11 +0.9 1,351,687 + psaE Synpcc7942_1322 0.32 -0.1 1,351,724 + psaE Synpcc7942_1322 0.49 -1.7 1,351,740 + psaE Synpcc7942_1322 0.56 -1.1 1,351,830 + -0.1 1,351,939 + -0.1 1,351,950 + -0.1 1,351,950 + -0.6 1,351,958 - -0.7 1,351,974 + -1.1 1,351,980 + -0.1 1,351,992 - +1.9 1,352,002 + +3.2 1,352,011 + -1.0 1,352,030 + -1.7 1,352,030 + +0.9 1,352,030 + -1.7 1,352,056 + fpg Synpcc7942_1323 0.13 -1.2 1,352,057 + fpg Synpcc7942_1323 0.13 -0.2 1,352,061 + fpg Synpcc7942_1323 0.13 +0.8 1,352,061 + fpg Synpcc7942_1323 0.13 +0.4 1,352,061 + fpg Synpcc7942_1323 0.13 -1.7 1,352,066 + fpg Synpcc7942_1323 0.14 +1.4 1,352,071 + fpg Synpcc7942_1323 0.14 +0.4 1,352,071 - fpg Synpcc7942_1323 0.14 -1.7 1,352,077 - fpg Synpcc7942_1323 0.15 -1.7 1,352,138 + fpg Synpcc7942_1323 0.22 +1.4 1,352,143 + fpg Synpcc7942_1323 0.23 +0.4 1,352,146 - fpg Synpcc7942_1323 0.23 +0.4 1,352,146 - fpg Synpcc7942_1323 0.23 -0.2 1,352,167 + fpg Synpcc7942_1323 0.26 -0.2 1,352,167 + fpg Synpcc7942_1323 0.26 -0.2 1,352,169 + fpg Synpcc7942_1323 0.26 +1.5 1,352,208 + fpg Synpcc7942_1323 0.31 +0.2 1,352,211 - fpg Synpcc7942_1323 0.31 +0.8 1,352,265 - fpg Synpcc7942_1323 0.37 +0.4 1,352,265 - -0.2 1,352,308 + fpg Synpcc7942_1323 0.42 -0.7 1,352,313 + fpg Synpcc7942_1323 0.43 -2.2 1,352,316 - fpg Synpcc7942_1323 0.43 -1.3 1,352,321 - fpg Synpcc7942_1323 0.44 -0.5 1,352,378 - fpg Synpcc7942_1323 0.51 +1.4 1,352,387 - fpg Synpcc7942_1323 0.52 -0.7 1,352,438 - fpg Synpcc7942_1323 0.58 +0.3 1,352,472 - fpg Synpcc7942_1323 0.62 +1.4 1,352,520 + fpg Synpcc7942_1323 0.67 +2.0 1,352,528 - fpg Synpcc7942_1323 0.68 -1.2 1,352,535 + fpg Synpcc7942_1323 0.69 -1.7 1,352,535 + fpg Synpcc7942_1323 0.69 -1.7 1,352,535 + fpg Synpcc7942_1323 0.69 -1.2 1,352,543 - fpg Synpcc7942_1323 0.70 -0.8 1,352,546 + fpg Synpcc7942_1323 0.70 -0.2 1,352,586 + fpg Synpcc7942_1323 0.75 -2.5 1,352,607 + fpg Synpcc7942_1323 0.78 -1.2 1,352,615 - fpg Synpcc7942_1323 0.78 +1.3 1,352,662 - fpg Synpcc7942_1323 0.84 +0.8 1,352,687 + fpg Synpcc7942_1323 0.87 -1.2 1,352,687 + fpg Synpcc7942_1323 0.87 +1.8 1,352,689 - fpg Synpcc7942_1323 0.87 +0.8 1,352,695 - fpg Synpcc7942_1323 0.88 -0.9 1,352,702 + -0.2 1,352,718 + -1.2 1,352,767 + -0.2 1,352,767 + -0.2 1,352,775 - +0.1 1,352,798 - +1.6 1,352,798 - +1.1 1,352,798 - +1.8 1,352,800 - -0.5 1,352,802 + +0.4 1,352,825 - -0.2 1,352,825 - -1.7 1,352,825 - +0.8 1,352,827 + +2.3 1,352,827 + +0.6 1,352,835 - -1.2
Or see this region's nucleotide sequence