Experiment: Spectinomycin 0.0009765625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpl21 and rpl27 are separated by 34 nucleotides rpl27 and cheY are separated by 121 nucleotides cheY and truB are separated by 92 nucleotides truB and Synpcc7942_1223 are separated by 20 nucleotides
Synpcc7942_1219: rpl21 - 50S ribosomal protein L21, at 1,241,649 to 1,242,032
rpl21
Synpcc7942_1220: rpl27 - 50S ribosomal protein L27, at 1,242,067 to 1,242,333
rpl27
Synpcc7942_1221: cheY - response regulator receiver domain protein (CheY-like), at 1,242,455 to 1,242,832
cheY
Synpcc7942_1222: truB - tRNA pseudouridine synthase B, at 1,242,925 to 1,243,809
truB
Synpcc7942_1223: Synpcc7942_1223 - DevC protein, at 1,243,830 to 1,245,008
_1223
Position (kb)
1242
1243
1244 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1242.306 kb on + strand, within rpl27 at 1242.366 kb on + strand at 1242.380 kb on - strand at 1242.513 kb on + strand, within cheY at 1242.556 kb on + strand, within cheY at 1242.563 kb on - strand, within cheY at 1242.716 kb on + strand, within cheY at 1242.795 kb on - strand at 1242.802 kb on - strand at 1242.814 kb on - strand at 1242.851 kb on - strand at 1242.862 kb on - strand at 1242.886 kb on - strand at 1242.904 kb on + strand at 1242.904 kb on + strand at 1242.912 kb on - strand at 1242.912 kb on - strand at 1242.929 kb on + strand at 1242.937 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.999 kb on - strand at 1243.008 kb on - strand at 1243.032 kb on - strand, within truB at 1243.092 kb on + strand, within truB at 1243.185 kb on + strand, within truB at 1243.288 kb on - strand, within truB at 1243.291 kb on - strand, within truB at 1243.342 kb on + strand, within truB at 1243.345 kb on - strand, within truB at 1243.355 kb on - strand, within truB at 1243.373 kb on - strand, within truB at 1243.436 kb on - strand, within truB at 1243.462 kb on + strand, within truB at 1243.470 kb on + strand, within truB at 1243.470 kb on + strand, within truB at 1243.470 kb on + strand, within truB at 1243.470 kb on + strand, within truB at 1243.478 kb on - strand, within truB at 1243.499 kb on + strand, within truB at 1243.507 kb on - strand, within truB at 1243.515 kb on - strand, within truB at 1243.532 kb on + strand, within truB at 1243.532 kb on + strand, within truB at 1243.532 kb on - strand at 1243.540 kb on - strand, within truB at 1243.556 kb on - strand, within truB at 1243.567 kb on - strand, within truB at 1243.598 kb on - strand, within truB at 1243.598 kb on - strand, within truB at 1243.638 kb on - strand, within truB at 1243.670 kb on + strand, within truB at 1243.674 kb on + strand, within truB at 1243.704 kb on - strand, within truB at 1243.706 kb on + strand, within truB at 1243.706 kb on + strand, within truB at 1243.719 kb on - strand, within truB at 1243.743 kb on + strand at 1243.751 kb on - strand at 1243.751 kb on - strand at 1243.774 kb on - strand at 1243.776 kb on + strand at 1243.782 kb on + strand at 1243.812 kb on - strand at 1243.853 kb on - strand at 1243.906 kb on - strand at 1243.918 kb on - strand at 1243.919 kb on - strand at 1243.938 kb on + strand at 1243.951 kb on - strand, within Synpcc7942_1223 at 1243.998 kb on - strand, within Synpcc7942_1223 at 1244.034 kb on - strand, within Synpcc7942_1223 at 1244.049 kb on - strand, within Synpcc7942_1223 at 1244.074 kb on + strand, within Synpcc7942_1223 at 1244.074 kb on + strand, within Synpcc7942_1223 at 1244.074 kb on + strand, within Synpcc7942_1223 at 1244.074 kb on + strand, within Synpcc7942_1223 at 1244.101 kb on - strand, within Synpcc7942_1223 at 1244.103 kb on - strand, within Synpcc7942_1223 at 1244.115 kb on + strand, within Synpcc7942_1223 at 1244.170 kb on - strand, within Synpcc7942_1223 at 1244.170 kb on - strand, within Synpcc7942_1223 at 1244.187 kb on + strand, within Synpcc7942_1223 at 1244.187 kb on + strand, within Synpcc7942_1223 at 1244.188 kb on - strand, within Synpcc7942_1223 at 1244.217 kb on - strand, within Synpcc7942_1223 at 1244.264 kb on + strand, within Synpcc7942_1223 at 1244.289 kb on + strand, within Synpcc7942_1223 at 1244.297 kb on - strand, within Synpcc7942_1223 at 1244.309 kb on + strand, within Synpcc7942_1223 at 1244.340 kb on - strand, within Synpcc7942_1223 at 1244.340 kb on - strand, within Synpcc7942_1223 at 1244.355 kb on - strand, within Synpcc7942_1223 at 1244.423 kb on - strand, within Synpcc7942_1223 at 1244.440 kb on + strand, within Synpcc7942_1223 at 1244.602 kb on + strand, within Synpcc7942_1223 at 1244.614 kb on + strand, within Synpcc7942_1223 at 1244.627 kb on - strand, within Synpcc7942_1223 at 1244.628 kb on - strand, within Synpcc7942_1223 at 1244.697 kb on + strand, within Synpcc7942_1223 at 1244.708 kb on + strand, within Synpcc7942_1223 at 1244.732 kb on + strand, within Synpcc7942_1223 at 1244.733 kb on + strand, within Synpcc7942_1223 at 1244.738 kb on + strand, within Synpcc7942_1223 at 1244.741 kb on - strand, within Synpcc7942_1223 at 1244.786 kb on - strand, within Synpcc7942_1223 at 1244.802 kb on + strand, within Synpcc7942_1223 at 1244.802 kb on + strand, within Synpcc7942_1223 at 1244.808 kb on - strand, within Synpcc7942_1223
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0009765625 mg/ml remove 1,242,306 + rpl27 Synpcc7942_1220 0.90 -1.8 1,242,366 + +1.2 1,242,380 - -0.2 1,242,513 + cheY Synpcc7942_1221 0.15 -1.2 1,242,556 + cheY Synpcc7942_1221 0.27 -0.4 1,242,563 - cheY Synpcc7942_1221 0.29 -0.2 1,242,716 + cheY Synpcc7942_1221 0.69 +1.1 1,242,795 - +0.8 1,242,802 - -0.2 1,242,814 - +1.4 1,242,851 - +0.8 1,242,862 - -0.2 1,242,886 - +2.2 1,242,904 + -0.2 1,242,904 + +0.8 1,242,912 - +2.0 1,242,912 - +1.4 1,242,929 + +0.9 1,242,937 + -0.5 1,242,973 + -1.2 1,242,973 + +1.8 1,242,973 + -0.2 1,242,973 + -0.8 1,242,973 + +1.8 1,242,973 + -1.2 1,242,973 + +2.2 1,242,973 + +4.5 1,242,981 - +1.4 1,242,981 - +2.4 1,242,981 - +0.4 1,242,981 - +2.2 1,242,981 - +1.4 1,242,981 - +0.4 1,242,981 - +1.2 1,242,999 - +0.6 1,243,008 - +1.7 1,243,032 - truB Synpcc7942_1222 0.12 +0.1 1,243,092 + truB Synpcc7942_1222 0.19 +2.4 1,243,185 + truB Synpcc7942_1222 0.29 +1.8 1,243,288 - truB Synpcc7942_1222 0.41 -0.2 1,243,291 - truB Synpcc7942_1222 0.41 +2.1 1,243,342 + truB Synpcc7942_1222 0.47 +2.2 1,243,345 - truB Synpcc7942_1222 0.47 +0.6 1,243,355 - truB Synpcc7942_1222 0.49 +0.8 1,243,373 - truB Synpcc7942_1222 0.51 -0.2 1,243,436 - truB Synpcc7942_1222 0.58 +1.4 1,243,462 + truB Synpcc7942_1222 0.61 -0.9 1,243,470 + truB Synpcc7942_1222 0.62 +3.2 1,243,470 + truB Synpcc7942_1222 0.62 +1.4 1,243,470 + truB Synpcc7942_1222 0.62 +1.4 1,243,470 + truB Synpcc7942_1222 0.62 +1.2 1,243,478 - truB Synpcc7942_1222 0.62 -0.2 1,243,499 + truB Synpcc7942_1222 0.65 -0.5 1,243,507 - truB Synpcc7942_1222 0.66 +0.1 1,243,515 - truB Synpcc7942_1222 0.67 +1.5 1,243,532 + truB Synpcc7942_1222 0.69 +1.3 1,243,532 + truB Synpcc7942_1222 0.69 +3.5 1,243,532 - +0.4 1,243,540 - truB Synpcc7942_1222 0.69 +1.4 1,243,556 - truB Synpcc7942_1222 0.71 +0.4 1,243,567 - truB Synpcc7942_1222 0.73 +3.3 1,243,598 - truB Synpcc7942_1222 0.76 +0.2 1,243,598 - truB Synpcc7942_1222 0.76 +0.8 1,243,638 - truB Synpcc7942_1222 0.81 -1.2 1,243,670 + truB Synpcc7942_1222 0.84 +0.6 1,243,674 + truB Synpcc7942_1222 0.85 +2.4 1,243,704 - truB Synpcc7942_1222 0.88 +1.1 1,243,706 + truB Synpcc7942_1222 0.88 +0.4 1,243,706 + truB Synpcc7942_1222 0.88 +0.8 1,243,719 - truB Synpcc7942_1222 0.90 -0.2 1,243,743 + +1.4 1,243,751 - +0.6 1,243,751 - +1.6 1,243,774 - +2.1 1,243,776 + -0.8 1,243,782 + -0.2 1,243,812 - +1.4 1,243,853 - -0.8 1,243,906 - -0.7 1,243,918 - -1.2 1,243,919 - +0.4 1,243,938 + +1.1 1,243,951 - Synpcc7942_1223 0.10 +0.4 1,243,998 - Synpcc7942_1223 0.14 +1.4 1,244,034 - Synpcc7942_1223 0.17 -1.8 1,244,049 - Synpcc7942_1223 0.19 +1.6 1,244,074 + Synpcc7942_1223 0.21 +0.8 1,244,074 + Synpcc7942_1223 0.21 +0.9 1,244,074 + Synpcc7942_1223 0.21 +0.6 1,244,074 + Synpcc7942_1223 0.21 -0.2 1,244,101 - Synpcc7942_1223 0.23 -0.2 1,244,103 - Synpcc7942_1223 0.23 +1.8 1,244,115 + Synpcc7942_1223 0.24 -0.2 1,244,170 - Synpcc7942_1223 0.29 +2.9 1,244,170 - Synpcc7942_1223 0.29 +0.4 1,244,187 + Synpcc7942_1223 0.30 -0.6 1,244,187 + Synpcc7942_1223 0.30 +0.2 1,244,188 - Synpcc7942_1223 0.30 -1.2 1,244,217 - Synpcc7942_1223 0.33 -1.3 1,244,264 + Synpcc7942_1223 0.37 -0.2 1,244,289 + Synpcc7942_1223 0.39 +0.3 1,244,297 - Synpcc7942_1223 0.40 -0.5 1,244,309 + Synpcc7942_1223 0.41 +1.2 1,244,340 - Synpcc7942_1223 0.43 +2.3 1,244,340 - Synpcc7942_1223 0.43 +3.1 1,244,355 - Synpcc7942_1223 0.45 +0.8 1,244,423 - Synpcc7942_1223 0.50 +1.4 1,244,440 + Synpcc7942_1223 0.52 -0.2 1,244,602 + Synpcc7942_1223 0.65 -1.2 1,244,614 + Synpcc7942_1223 0.66 +0.5 1,244,627 - Synpcc7942_1223 0.68 -0.2 1,244,628 - Synpcc7942_1223 0.68 -0.2 1,244,697 + Synpcc7942_1223 0.74 +1.1 1,244,708 + Synpcc7942_1223 0.74 +1.8 1,244,732 + Synpcc7942_1223 0.77 -0.3 1,244,733 + Synpcc7942_1223 0.77 -0.2 1,244,738 + Synpcc7942_1223 0.77 +0.8 1,244,741 - Synpcc7942_1223 0.77 +1.4 1,244,786 - Synpcc7942_1223 0.81 -0.2 1,244,802 + Synpcc7942_1223 0.82 +2.2 1,244,802 + Synpcc7942_1223 0.82 +2.2 1,244,808 - Synpcc7942_1223 0.83 +0.2
Or see this region's nucleotide sequence