Experiment: Spectinomycin 0.0009765625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemC and Synpcc7942_0968 are separated by 81 nucleotides Synpcc7942_0968 and Synpcc7942_0969 are separated by 9 nucleotides Synpcc7942_0969 and crtD are separated by 115 nucleotides
Synpcc7942_0967: hemC - porphobilinogen deaminase, at 974,099 to 975,061
hemC
Synpcc7942_0968: Synpcc7942_0968 - hypothetical protein, at 975,143 to 975,358
_0968
Synpcc7942_0969: Synpcc7942_0969 - Carboxymethylenebutenolidase, at 975,368 to 976,195
_0969
Synpcc7942_0970: crtD - phytoene dehydrogenase-like, at 976,311 to 977,882
crtD
Position (kb) 975
976 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 975.116 kb on + strand at 975.116 kb on + strand at 975.116 kb on + strand at 975.126 kb on + strand at 975.334 kb on - strand, within Synpcc7942_0968 at 975.382 kb on + strand at 975.382 kb on + strand at 975.382 kb on + strand at 975.382 kb on + strand at 975.390 kb on - strand at 975.393 kb on + strand at 975.437 kb on - strand at 975.441 kb on + strand at 975.451 kb on - strand, within Synpcc7942_0969 at 975.461 kb on + strand, within Synpcc7942_0969 at 975.508 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.518 kb on - strand, within Synpcc7942_0969 at 975.530 kb on + strand, within Synpcc7942_0969 at 975.538 kb on - strand, within Synpcc7942_0969 at 975.554 kb on + strand, within Synpcc7942_0969 at 975.555 kb on + strand, within Synpcc7942_0969 at 975.555 kb on + strand, within Synpcc7942_0969 at 975.562 kb on - strand, within Synpcc7942_0969 at 975.563 kb on - strand, within Synpcc7942_0969 at 975.578 kb on + strand, within Synpcc7942_0969 at 975.591 kb on - strand, within Synpcc7942_0969 at 975.608 kb on + strand, within Synpcc7942_0969 at 975.618 kb on + strand, within Synpcc7942_0969 at 975.630 kb on - strand, within Synpcc7942_0969 at 975.634 kb on + strand, within Synpcc7942_0969 at 975.662 kb on + strand, within Synpcc7942_0969 at 975.686 kb on - strand, within Synpcc7942_0969 at 975.702 kb on + strand, within Synpcc7942_0969 at 975.702 kb on + strand, within Synpcc7942_0969 at 975.721 kb on + strand, within Synpcc7942_0969 at 975.731 kb on + strand, within Synpcc7942_0969 at 975.767 kb on - strand, within Synpcc7942_0969 at 975.782 kb on - strand, within Synpcc7942_0969 at 975.833 kb on + strand, within Synpcc7942_0969 at 975.898 kb on + strand, within Synpcc7942_0969 at 975.898 kb on + strand, within Synpcc7942_0969 at 975.906 kb on - strand, within Synpcc7942_0969 at 975.925 kb on + strand, within Synpcc7942_0969 at 975.947 kb on + strand, within Synpcc7942_0969 at 976.038 kb on + strand, within Synpcc7942_0969 at 976.043 kb on - strand, within Synpcc7942_0969 at 976.053 kb on - strand, within Synpcc7942_0969 at 976.078 kb on + strand, within Synpcc7942_0969 at 976.087 kb on - strand, within Synpcc7942_0969 at 976.087 kb on - strand, within Synpcc7942_0969 at 976.098 kb on + strand, within Synpcc7942_0969 at 976.130 kb on + strand at 976.135 kb on + strand at 976.135 kb on + strand at 976.154 kb on - strand at 976.162 kb on - strand at 976.206 kb on - strand at 976.206 kb on - strand at 976.206 kb on - strand at 976.206 kb on - strand at 976.206 kb on - strand at 976.254 kb on + strand at 976.262 kb on - strand at 976.314 kb on + strand at 976.323 kb on - strand at 976.325 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0009765625 mg/ml remove 975,116 + +3.7 975,116 + -2.4 975,116 + +0.6 975,126 + -0.8 975,334 - Synpcc7942_0968 0.88 -1.6 975,382 + +0.5 975,382 + +0.5 975,382 + -0.1 975,382 + -1.7 975,390 - +0.9 975,393 + -2.3 975,437 - -1.7 975,441 + -1.1 975,451 - Synpcc7942_0969 0.10 -1.1 975,461 + Synpcc7942_0969 0.11 +2.1 975,508 - Synpcc7942_0969 0.17 -0.1 975,518 - Synpcc7942_0969 0.18 -0.1 975,518 - Synpcc7942_0969 0.18 +0.5 975,518 - Synpcc7942_0969 0.18 -2.1 975,518 - Synpcc7942_0969 0.18 +0.5 975,518 - Synpcc7942_0969 0.18 -1.1 975,518 - Synpcc7942_0969 0.18 -2.1 975,530 + Synpcc7942_0969 0.20 +2.0 975,538 - Synpcc7942_0969 0.21 -0.7 975,554 + Synpcc7942_0969 0.22 -1.1 975,555 + Synpcc7942_0969 0.23 +0.2 975,555 + Synpcc7942_0969 0.23 -1.1 975,562 - Synpcc7942_0969 0.23 -0.1 975,563 - Synpcc7942_0969 0.24 -1.7 975,578 + Synpcc7942_0969 0.25 -0.1 975,591 - Synpcc7942_0969 0.27 -1.4 975,608 + Synpcc7942_0969 0.29 +0.9 975,618 + Synpcc7942_0969 0.30 -1.1 975,630 - Synpcc7942_0969 0.32 -0.8 975,634 + Synpcc7942_0969 0.32 +0.5 975,662 + Synpcc7942_0969 0.36 +0.7 975,686 - Synpcc7942_0969 0.38 -1.7 975,702 + Synpcc7942_0969 0.40 -1.1 975,702 + Synpcc7942_0969 0.40 -0.1 975,721 + Synpcc7942_0969 0.43 -0.1 975,731 + Synpcc7942_0969 0.44 -0.3 975,767 - Synpcc7942_0969 0.48 -0.1 975,782 - Synpcc7942_0969 0.50 -0.1 975,833 + Synpcc7942_0969 0.56 +0.9 975,898 + Synpcc7942_0969 0.64 -0.8 975,898 + Synpcc7942_0969 0.64 -0.1 975,906 - Synpcc7942_0969 0.65 -0.9 975,925 + Synpcc7942_0969 0.67 -1.4 975,947 + Synpcc7942_0969 0.70 -0.1 976,038 + Synpcc7942_0969 0.81 +0.6 976,043 - Synpcc7942_0969 0.82 -0.8 976,053 - Synpcc7942_0969 0.83 +0.4 976,078 + Synpcc7942_0969 0.86 +0.9 976,087 - Synpcc7942_0969 0.87 -1.4 976,087 - Synpcc7942_0969 0.87 +2.1 976,098 + Synpcc7942_0969 0.88 -0.1 976,130 + -0.5 976,135 + -1.7 976,135 + -0.1 976,154 - +2.6 976,162 - -1.1 976,206 - +0.3 976,206 - -1.7 976,206 - +1.2 976,206 - -0.1 976,206 - -1.1 976,254 + -1.1 976,262 - -1.7 976,314 + -1.7 976,323 - -0.1 976,325 + -0.1
Or see this region's nucleotide sequence