Experiment: Spectinomycin 0.0009765625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrB and Synpcc7942_0671 are separated by 18 nucleotides Synpcc7942_0671 and rpoD3 are separated by 233 nucleotides rpoD3 and mutT overlap by 1 nucleotides
Synpcc7942_0670: pyrB - aspartate carbamoyltransferase catalytic subunit, at 665,121 to 666,137
pyrB
Synpcc7942_0671: Synpcc7942_0671 - hypothetical protein, at 666,156 to 666,653
_0671
Synpcc7942_0672: rpoD3 - RNA polymerase sigma factor SigD, at 666,887 to 667,849
rpoD3
Synpcc7942_0673: mutT - A/G-specific DNA-adenine glycosylase, at 667,849 to 668,931
mutT
Position (kb) 666
667
668 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 666.080 kb on + strand at 666.106 kb on + strand at 666.109 kb on - strand at 666.114 kb on - strand at 666.119 kb on - strand at 666.159 kb on + strand at 666.202 kb on + strand at 666.205 kb on - strand at 666.205 kb on - strand at 666.210 kb on - strand, within Synpcc7942_0671 at 666.229 kb on + strand, within Synpcc7942_0671 at 666.229 kb on + strand, within Synpcc7942_0671 at 666.252 kb on - strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.264 kb on - strand, within Synpcc7942_0671 at 666.264 kb on - strand, within Synpcc7942_0671 at 666.324 kb on - strand, within Synpcc7942_0671 at 666.332 kb on + strand, within Synpcc7942_0671 at 666.340 kb on - strand at 666.348 kb on - strand, within Synpcc7942_0671 at 666.377 kb on + strand, within Synpcc7942_0671 at 666.377 kb on + strand, within Synpcc7942_0671 at 666.395 kb on - strand, within Synpcc7942_0671 at 666.396 kb on - strand at 666.407 kb on + strand, within Synpcc7942_0671 at 666.407 kb on + strand, within Synpcc7942_0671 at 666.407 kb on + strand, within Synpcc7942_0671 at 666.407 kb on + strand, within Synpcc7942_0671 at 666.414 kb on + strand, within Synpcc7942_0671 at 666.415 kb on - strand, within Synpcc7942_0671 at 666.415 kb on - strand, within Synpcc7942_0671 at 666.422 kb on - strand, within Synpcc7942_0671 at 666.426 kb on + strand, within Synpcc7942_0671 at 666.431 kb on + strand, within Synpcc7942_0671 at 666.476 kb on + strand, within Synpcc7942_0671 at 666.476 kb on + strand, within Synpcc7942_0671 at 666.476 kb on + strand, within Synpcc7942_0671 at 666.484 kb on - strand, within Synpcc7942_0671 at 666.484 kb on - strand, within Synpcc7942_0671 at 666.484 kb on - strand, within Synpcc7942_0671 at 666.489 kb on - strand, within Synpcc7942_0671 at 666.497 kb on - strand, within Synpcc7942_0671 at 666.499 kb on + strand, within Synpcc7942_0671 at 666.535 kb on + strand, within Synpcc7942_0671 at 666.535 kb on + strand, within Synpcc7942_0671 at 666.538 kb on + strand, within Synpcc7942_0671 at 666.545 kb on + strand at 666.633 kb on - strand at 666.635 kb on + strand at 666.638 kb on - strand at 666.656 kb on - strand at 666.676 kb on + strand at 666.676 kb on - strand at 666.683 kb on + strand at 666.684 kb on - strand at 666.691 kb on - strand at 666.752 kb on - strand at 666.763 kb on - strand at 666.784 kb on - strand at 666.872 kb on + strand at 666.910 kb on + strand at 666.920 kb on + strand at 666.924 kb on + strand at 666.924 kb on + strand at 666.924 kb on + strand at 666.956 kb on + strand at 666.964 kb on - strand at 666.971 kb on + strand at 666.993 kb on + strand, within rpoD3 at 667.000 kb on + strand, within rpoD3 at 667.038 kb on + strand, within rpoD3 at 667.046 kb on - strand, within rpoD3 at 667.046 kb on - strand, within rpoD3 at 667.046 kb on - strand, within rpoD3 at 667.072 kb on + strand, within rpoD3 at 667.106 kb on + strand, within rpoD3 at 667.115 kb on + strand, within rpoD3 at 667.123 kb on + strand, within rpoD3 at 667.178 kb on - strand, within rpoD3 at 667.245 kb on + strand, within rpoD3 at 667.253 kb on - strand, within rpoD3 at 667.370 kb on - strand at 667.385 kb on + strand, within rpoD3 at 667.479 kb on + strand, within rpoD3 at 667.485 kb on + strand, within rpoD3 at 667.485 kb on + strand, within rpoD3 at 667.485 kb on - strand, within rpoD3 at 667.488 kb on + strand, within rpoD3 at 667.493 kb on - strand, within rpoD3 at 667.704 kb on + strand, within rpoD3 at 667.704 kb on + strand, within rpoD3 at 667.704 kb on + strand, within rpoD3 at 667.704 kb on - strand, within rpoD3 at 667.704 kb on - strand, within rpoD3 at 667.706 kb on + strand, within rpoD3 at 667.712 kb on - strand, within rpoD3 at 667.721 kb on + strand, within rpoD3 at 667.729 kb on - strand, within rpoD3 at 667.729 kb on - strand, within rpoD3 at 667.772 kb on - strand at 667.797 kb on + strand at 667.803 kb on - strand at 667.805 kb on - strand at 667.805 kb on - strand at 667.805 kb on - strand at 667.868 kb on + strand at 667.868 kb on + strand at 667.868 kb on + strand at 667.868 kb on + strand at 667.876 kb on - strand at 667.876 kb on - strand at 667.876 kb on - strand at 667.888 kb on + strand at 667.893 kb on + strand at 667.906 kb on + strand at 667.921 kb on - strand at 667.923 kb on + strand at 667.944 kb on + strand at 667.944 kb on + strand at 667.952 kb on - strand at 667.952 kb on - strand at 667.967 kb on + strand, within mutT at 668.016 kb on + strand, within mutT at 668.016 kb on + strand, within mutT at 668.024 kb on - strand, within mutT at 668.024 kb on - strand, within mutT at 668.039 kb on + strand, within mutT at 668.039 kb on + strand, within mutT at 668.047 kb on - strand, within mutT at 668.047 kb on - strand, within mutT at 668.049 kb on - strand, within mutT at 668.055 kb on - strand, within mutT at 668.062 kb on + strand, within mutT at 668.084 kb on - strand, within mutT at 668.092 kb on + strand, within mutT at 668.092 kb on + strand, within mutT at 668.092 kb on + strand, within mutT at 668.110 kb on - strand, within mutT at 668.111 kb on - strand, within mutT at 668.118 kb on + strand, within mutT at 668.119 kb on + strand, within mutT at 668.142 kb on - strand, within mutT at 668.150 kb on - strand, within mutT at 668.163 kb on - strand, within mutT at 668.207 kb on - strand, within mutT at 668.217 kb on - strand, within mutT at 668.219 kb on - strand, within mutT at 668.250 kb on - strand, within mutT at 668.305 kb on + strand, within mutT at 668.313 kb on - strand, within mutT at 668.340 kb on + strand, within mutT at 668.351 kb on + strand, within mutT at 668.351 kb on + strand, within mutT at 668.359 kb on - strand, within mutT at 668.359 kb on - strand, within mutT at 668.364 kb on + strand, within mutT at 668.369 kb on + strand, within mutT at 668.382 kb on - strand, within mutT at 668.387 kb on + strand, within mutT at 668.401 kb on + strand, within mutT at 668.405 kb on - strand, within mutT at 668.409 kb on - strand, within mutT at 668.433 kb on + strand, within mutT at 668.441 kb on - strand at 668.441 kb on - strand, within mutT at 668.441 kb on - strand, within mutT at 668.443 kb on - strand, within mutT at 668.487 kb on - strand, within mutT at 668.496 kb on - strand, within mutT at 668.517 kb on - strand, within mutT at 668.536 kb on - strand, within mutT at 668.591 kb on - strand, within mutT at 668.649 kb on + strand, within mutT at 668.666 kb on + strand, within mutT at 668.667 kb on - strand, within mutT at 668.761 kb on - strand, within mutT
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0009765625 mg/ml remove 666,080 + +0.8 666,106 + -1.2 666,109 - +0.1 666,114 - -0.2 666,119 - +2.5 666,159 + -0.2 666,202 + -1.2 666,205 - -0.2 666,205 - +0.8 666,210 - Synpcc7942_0671 0.11 +1.4 666,229 + Synpcc7942_0671 0.15 -1.3 666,229 + Synpcc7942_0671 0.15 -0.2 666,252 - Synpcc7942_0671 0.19 -0.2 666,256 + Synpcc7942_0671 0.20 -2.5 666,256 + Synpcc7942_0671 0.20 +1.4 666,256 + Synpcc7942_0671 0.20 -1.6 666,256 + Synpcc7942_0671 0.20 -0.2 666,264 - Synpcc7942_0671 0.22 +1.0 666,264 - Synpcc7942_0671 0.22 -1.8 666,324 - Synpcc7942_0671 0.34 +0.3 666,332 + Synpcc7942_0671 0.35 -0.4 666,340 - +0.8 666,348 - Synpcc7942_0671 0.39 -0.2 666,377 + Synpcc7942_0671 0.44 +0.8 666,377 + Synpcc7942_0671 0.44 -0.2 666,395 - Synpcc7942_0671 0.48 -0.2 666,396 - +0.4 666,407 + Synpcc7942_0671 0.50 +0.6 666,407 + Synpcc7942_0671 0.50 -0.6 666,407 + Synpcc7942_0671 0.50 -1.8 666,407 + Synpcc7942_0671 0.50 +2.1 666,414 + Synpcc7942_0671 0.52 +0.4 666,415 - Synpcc7942_0671 0.52 +0.7 666,415 - Synpcc7942_0671 0.52 +0.8 666,422 - Synpcc7942_0671 0.53 +2.1 666,426 + Synpcc7942_0671 0.54 -0.2 666,431 + Synpcc7942_0671 0.55 +0.9 666,476 + Synpcc7942_0671 0.64 +0.8 666,476 + Synpcc7942_0671 0.64 -0.6 666,476 + Synpcc7942_0671 0.64 -1.2 666,484 - Synpcc7942_0671 0.66 -0.2 666,484 - Synpcc7942_0671 0.66 -1.8 666,484 - Synpcc7942_0671 0.66 +2.3 666,489 - Synpcc7942_0671 0.67 +0.8 666,497 - Synpcc7942_0671 0.68 -1.2 666,499 + Synpcc7942_0671 0.69 -0.9 666,535 + Synpcc7942_0671 0.76 +2.4 666,535 + Synpcc7942_0671 0.76 -0.8 666,538 + Synpcc7942_0671 0.77 +0.1 666,545 + -0.2 666,633 - +0.0 666,635 + +0.0 666,638 - +1.3 666,656 - +1.0 666,676 + -0.9 666,676 - +0.8 666,683 + +0.8 666,684 - +0.8 666,691 - -0.2 666,752 - +0.2 666,763 - +2.6 666,784 - +3.8 666,872 + +0.8 666,910 + +0.8 666,920 + -1.8 666,924 + -0.2 666,924 + -1.2 666,924 + -1.2 666,956 + -0.2 666,964 - +0.8 666,971 + -1.8 666,993 + rpoD3 Synpcc7942_0672 0.11 -0.2 667,000 + rpoD3 Synpcc7942_0672 0.12 -1.4 667,038 + rpoD3 Synpcc7942_0672 0.16 -0.2 667,046 - rpoD3 Synpcc7942_0672 0.17 +1.4 667,046 - rpoD3 Synpcc7942_0672 0.17 -0.2 667,046 - rpoD3 Synpcc7942_0672 0.17 +0.1 667,072 + rpoD3 Synpcc7942_0672 0.19 -1.8 667,106 + rpoD3 Synpcc7942_0672 0.23 -1.8 667,115 + rpoD3 Synpcc7942_0672 0.24 -0.2 667,123 + rpoD3 Synpcc7942_0672 0.25 -1.5 667,178 - rpoD3 Synpcc7942_0672 0.30 -2.2 667,245 + rpoD3 Synpcc7942_0672 0.37 +0.8 667,253 - rpoD3 Synpcc7942_0672 0.38 +0.1 667,370 - -0.2 667,385 + rpoD3 Synpcc7942_0672 0.52 -1.2 667,479 + rpoD3 Synpcc7942_0672 0.61 -1.2 667,485 + rpoD3 Synpcc7942_0672 0.62 -1.5 667,485 + rpoD3 Synpcc7942_0672 0.62 -1.4 667,485 - rpoD3 Synpcc7942_0672 0.62 -0.2 667,488 + rpoD3 Synpcc7942_0672 0.62 -1.3 667,493 - rpoD3 Synpcc7942_0672 0.63 -2.5 667,704 + rpoD3 Synpcc7942_0672 0.85 -1.2 667,704 + rpoD3 Synpcc7942_0672 0.85 -0.6 667,704 + rpoD3 Synpcc7942_0672 0.85 -2.0 667,704 - rpoD3 Synpcc7942_0672 0.85 -0.2 667,704 - rpoD3 Synpcc7942_0672 0.85 -0.8 667,706 + rpoD3 Synpcc7942_0672 0.85 -0.2 667,712 - rpoD3 Synpcc7942_0672 0.86 +1.4 667,721 + rpoD3 Synpcc7942_0672 0.87 +2.6 667,729 - rpoD3 Synpcc7942_0672 0.87 -2.4 667,729 - rpoD3 Synpcc7942_0672 0.87 -1.2 667,772 - -0.2 667,797 + -2.2 667,803 - -1.8 667,805 - -1.8 667,805 - -0.2 667,805 - -1.5 667,868 + +0.8 667,868 + -0.8 667,868 + -1.8 667,868 + -0.7 667,876 - -2.2 667,876 - +0.4 667,876 - -1.2 667,888 + -0.2 667,893 + -1.5 667,906 + -0.2 667,921 - +0.8 667,923 + +0.8 667,944 + -1.2 667,944 + +0.2 667,952 - -0.2 667,952 - -0.2 667,967 + mutT Synpcc7942_0673 0.11 +0.4 668,016 + mutT Synpcc7942_0673 0.15 -1.2 668,016 + mutT Synpcc7942_0673 0.15 -0.5 668,024 - mutT Synpcc7942_0673 0.16 -0.2 668,024 - mutT Synpcc7942_0673 0.16 -0.9 668,039 + mutT Synpcc7942_0673 0.18 -1.8 668,039 + mutT Synpcc7942_0673 0.18 +0.8 668,047 - mutT Synpcc7942_0673 0.18 +0.4 668,047 - mutT Synpcc7942_0673 0.18 -1.5 668,049 - mutT Synpcc7942_0673 0.18 +1.7 668,055 - mutT Synpcc7942_0673 0.19 +1.4 668,062 + mutT Synpcc7942_0673 0.20 +0.4 668,084 - mutT Synpcc7942_0673 0.22 +1.1 668,092 + mutT Synpcc7942_0673 0.22 -1.2 668,092 + mutT Synpcc7942_0673 0.22 -0.6 668,092 + mutT Synpcc7942_0673 0.22 -0.2 668,110 - mutT Synpcc7942_0673 0.24 +1.4 668,111 - mutT Synpcc7942_0673 0.24 -0.1 668,118 + mutT Synpcc7942_0673 0.25 -0.2 668,119 + mutT Synpcc7942_0673 0.25 +1.4 668,142 - mutT Synpcc7942_0673 0.27 +1.2 668,150 - mutT Synpcc7942_0673 0.28 -1.2 668,163 - mutT Synpcc7942_0673 0.29 +0.4 668,207 - mutT Synpcc7942_0673 0.33 +1.8 668,217 - mutT Synpcc7942_0673 0.34 -2.2 668,219 - mutT Synpcc7942_0673 0.34 +0.8 668,250 - mutT Synpcc7942_0673 0.37 +2.3 668,305 + mutT Synpcc7942_0673 0.42 +1.4 668,313 - mutT Synpcc7942_0673 0.43 +1.4 668,340 + mutT Synpcc7942_0673 0.45 -0.8 668,351 + mutT Synpcc7942_0673 0.46 +0.8 668,351 + mutT Synpcc7942_0673 0.46 +0.8 668,359 - mutT Synpcc7942_0673 0.47 -1.6 668,359 - mutT Synpcc7942_0673 0.47 -1.2 668,364 + mutT Synpcc7942_0673 0.48 +0.8 668,369 + mutT Synpcc7942_0673 0.48 -1.2 668,382 - mutT Synpcc7942_0673 0.49 -1.8 668,387 + mutT Synpcc7942_0673 0.50 -1.7 668,401 + mutT Synpcc7942_0673 0.51 +0.0 668,405 - mutT Synpcc7942_0673 0.51 -0.5 668,409 - mutT Synpcc7942_0673 0.52 +0.2 668,433 + mutT Synpcc7942_0673 0.54 -0.2 668,441 - -0.2 668,441 - mutT Synpcc7942_0673 0.55 -0.2 668,441 - mutT Synpcc7942_0673 0.55 -0.4 668,443 - mutT Synpcc7942_0673 0.55 -0.2 668,487 - mutT Synpcc7942_0673 0.59 -3.0 668,496 - mutT Synpcc7942_0673 0.60 -1.2 668,517 - mutT Synpcc7942_0673 0.62 -1.8 668,536 - mutT Synpcc7942_0673 0.63 +0.9 668,591 - mutT Synpcc7942_0673 0.69 +0.7 668,649 + mutT Synpcc7942_0673 0.74 +0.8 668,666 + mutT Synpcc7942_0673 0.75 +0.4 668,667 - mutT Synpcc7942_0673 0.76 -1.4 668,761 - mutT Synpcc7942_0673 0.84 -1.2
Or see this region's nucleotide sequence