Experiment: Spectinomycin 0.0009765625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0622 and trxB are separated by 59 nucleotides trxB and ldpA are separated by 9 nucleotides
Synpcc7942_0622: Synpcc7942_0622 - ATPase, at 611,915 to 613,912
_0622
Synpcc7942_0623: trxB - thioredoxin reductase, at 613,972 to 615,351
trxB
Synpcc7942_0624: ldpA - light dependent period, at 615,361 to 616,419
ldpA
Position (kb) 613
614
615
616 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 613.218 kb on + strand, within Synpcc7942_0622 at 613.223 kb on - strand, within Synpcc7942_0622 at 613.241 kb on - strand, within Synpcc7942_0622 at 613.248 kb on - strand, within Synpcc7942_0622 at 613.291 kb on - strand, within Synpcc7942_0622 at 613.467 kb on - strand, within Synpcc7942_0622 at 613.467 kb on - strand, within Synpcc7942_0622 at 613.490 kb on - strand, within Synpcc7942_0622 at 613.519 kb on + strand, within Synpcc7942_0622 at 613.524 kb on + strand, within Synpcc7942_0622 at 613.527 kb on + strand, within Synpcc7942_0622 at 613.528 kb on - strand, within Synpcc7942_0622 at 613.626 kb on + strand, within Synpcc7942_0622 at 613.642 kb on - strand, within Synpcc7942_0622 at 613.770 kb on - strand at 613.811 kb on - strand at 613.815 kb on + strand at 613.815 kb on + strand at 613.815 kb on + strand at 613.823 kb on - strand at 613.866 kb on - strand at 613.894 kb on + strand at 613.907 kb on + strand at 613.916 kb on + strand at 613.932 kb on - strand at 614.041 kb on + strand at 614.042 kb on - strand at 614.067 kb on - strand at 614.067 kb on - strand at 614.106 kb on + strand at 614.125 kb on - strand, within trxB at 614.142 kb on + strand, within trxB at 614.159 kb on - strand, within trxB at 614.170 kb on - strand, within trxB at 614.180 kb on - strand, within trxB at 614.187 kb on + strand, within trxB at 614.207 kb on + strand, within trxB at 614.214 kb on + strand, within trxB at 614.337 kb on + strand, within trxB at 614.394 kb on + strand at 614.606 kb on - strand, within trxB at 614.610 kb on + strand, within trxB at 614.622 kb on + strand, within trxB at 614.630 kb on - strand, within trxB at 614.630 kb on - strand, within trxB at 614.630 kb on - strand, within trxB at 614.630 kb on - strand, within trxB at 614.780 kb on - strand, within trxB at 614.821 kb on + strand, within trxB at 614.899 kb on - strand, within trxB at 614.966 kb on - strand, within trxB at 615.004 kb on + strand, within trxB at 615.034 kb on - strand, within trxB at 615.044 kb on - strand, within trxB at 615.046 kb on - strand, within trxB at 615.048 kb on + strand, within trxB at 615.048 kb on + strand, within trxB at 615.095 kb on - strand, within trxB at 615.097 kb on + strand, within trxB at 615.130 kb on - strand, within trxB at 615.166 kb on + strand, within trxB at 615.174 kb on - strand, within trxB at 615.174 kb on - strand, within trxB at 615.242 kb on + strand at 615.261 kb on - strand at 615.264 kb on + strand at 615.264 kb on + strand at 615.344 kb on + strand at 615.351 kb on + strand at 615.353 kb on + strand at 615.353 kb on + strand at 615.386 kb on + strand at 615.396 kb on - strand at 615.404 kb on + strand at 615.467 kb on + strand, within ldpA at 615.467 kb on + strand, within ldpA at 615.474 kb on - strand, within ldpA at 615.475 kb on - strand, within ldpA at 615.519 kb on + strand, within ldpA at 615.593 kb on + strand, within ldpA at 615.593 kb on + strand, within ldpA at 615.658 kb on - strand, within ldpA at 615.662 kb on + strand, within ldpA at 615.670 kb on - strand, within ldpA at 615.670 kb on - strand, within ldpA at 615.670 kb on - strand, within ldpA at 615.751 kb on + strand, within ldpA at 615.786 kb on + strand, within ldpA at 615.902 kb on + strand, within ldpA at 615.975 kb on + strand, within ldpA at 615.983 kb on - strand, within ldpA at 616.075 kb on - strand, within ldpA at 616.089 kb on - strand, within ldpA at 616.093 kb on + strand, within ldpA at 616.195 kb on + strand, within ldpA at 616.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0009765625 mg/ml remove 613,218 + Synpcc7942_0622 0.65 +5.4 613,223 - Synpcc7942_0622 0.65 -0.2 613,241 - Synpcc7942_0622 0.66 +3.0 613,248 - Synpcc7942_0622 0.67 +5.8 613,291 - Synpcc7942_0622 0.69 +4.5 613,467 - Synpcc7942_0622 0.78 +5.7 613,467 - Synpcc7942_0622 0.78 +3.7 613,490 - Synpcc7942_0622 0.79 -1.8 613,519 + Synpcc7942_0622 0.80 +2.5 613,524 + Synpcc7942_0622 0.81 +1.7 613,527 + Synpcc7942_0622 0.81 +3.6 613,528 - Synpcc7942_0622 0.81 -1.2 613,626 + Synpcc7942_0622 0.86 +0.3 613,642 - Synpcc7942_0622 0.86 +0.4 613,770 - +1.4 613,811 - +6.1 613,815 + -1.2 613,815 + +1.6 613,815 + +0.2 613,823 - -0.8 613,866 - +1.7 613,894 + +6.9 613,907 + +1.1 613,916 + +1.8 613,932 - -0.6 614,041 + -0.2 614,042 - +0.8 614,067 - -1.8 614,067 - -1.5 614,106 + -2.2 614,125 - trxB Synpcc7942_0623 0.11 +0.8 614,142 + trxB Synpcc7942_0623 0.12 +0.8 614,159 - trxB Synpcc7942_0623 0.14 +0.6 614,170 - trxB Synpcc7942_0623 0.14 -2.8 614,180 - trxB Synpcc7942_0623 0.15 -2.2 614,187 + trxB Synpcc7942_0623 0.16 -0.2 614,207 + trxB Synpcc7942_0623 0.17 -1.2 614,214 + trxB Synpcc7942_0623 0.18 -0.2 614,337 + trxB Synpcc7942_0623 0.26 +0.8 614,394 + -0.2 614,606 - trxB Synpcc7942_0623 0.46 -1.5 614,610 + trxB Synpcc7942_0623 0.46 -0.2 614,622 + trxB Synpcc7942_0623 0.47 -1.2 614,630 - trxB Synpcc7942_0623 0.48 -0.2 614,630 - trxB Synpcc7942_0623 0.48 -0.2 614,630 - trxB Synpcc7942_0623 0.48 -1.2 614,630 - trxB Synpcc7942_0623 0.48 -0.2 614,780 - trxB Synpcc7942_0623 0.59 -2.2 614,821 + trxB Synpcc7942_0623 0.62 -0.2 614,899 - trxB Synpcc7942_0623 0.67 -2.2 614,966 - trxB Synpcc7942_0623 0.72 -1.2 615,004 + trxB Synpcc7942_0623 0.75 -1.2 615,034 - trxB Synpcc7942_0623 0.77 -0.7 615,044 - trxB Synpcc7942_0623 0.78 -1.2 615,046 - trxB Synpcc7942_0623 0.78 -0.2 615,048 + trxB Synpcc7942_0623 0.78 -2.2 615,048 + trxB Synpcc7942_0623 0.78 -1.8 615,095 - trxB Synpcc7942_0623 0.81 -0.2 615,097 + trxB Synpcc7942_0623 0.82 -0.2 615,130 - trxB Synpcc7942_0623 0.84 -1.8 615,166 + trxB Synpcc7942_0623 0.87 -1.2 615,174 - trxB Synpcc7942_0623 0.87 -0.8 615,174 - trxB Synpcc7942_0623 0.87 +0.8 615,242 + -0.2 615,261 - -1.2 615,264 + -2.5 615,264 + -1.2 615,344 + -0.2 615,351 + -1.8 615,353 + -2.2 615,353 + -1.6 615,386 + -1.2 615,396 - -0.2 615,404 + -2.5 615,467 + ldpA Synpcc7942_0624 0.10 -2.2 615,467 + ldpA Synpcc7942_0624 0.10 -3.6 615,474 - ldpA Synpcc7942_0624 0.11 -1.5 615,475 - ldpA Synpcc7942_0624 0.11 -2.2 615,519 + ldpA Synpcc7942_0624 0.15 +0.8 615,593 + ldpA Synpcc7942_0624 0.22 -2.5 615,593 + ldpA Synpcc7942_0624 0.22 -2.2 615,658 - ldpA Synpcc7942_0624 0.28 -1.2 615,662 + ldpA Synpcc7942_0624 0.28 -2.4 615,670 - ldpA Synpcc7942_0624 0.29 -1.2 615,670 - ldpA Synpcc7942_0624 0.29 -1.2 615,670 - ldpA Synpcc7942_0624 0.29 -1.2 615,751 + ldpA Synpcc7942_0624 0.37 -1.2 615,786 + ldpA Synpcc7942_0624 0.40 -2.2 615,902 + ldpA Synpcc7942_0624 0.51 +0.8 615,975 + ldpA Synpcc7942_0624 0.58 -1.2 615,983 - ldpA Synpcc7942_0624 0.59 -2.2 616,075 - ldpA Synpcc7942_0624 0.67 -1.2 616,089 - ldpA Synpcc7942_0624 0.69 -1.2 616,093 + ldpA Synpcc7942_0624 0.69 -2.5 616,195 + ldpA Synpcc7942_0624 0.79 -2.9 616,321 + -1.2
Or see this region's nucleotide sequence