Experiment: Spectinomycin 0.0009765625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pqqE and rpoD4 are separated by 0 nucleotides rpoD4 and ycf63 are separated by 325 nucleotides ycf63 and Synpcc7942_0379 are separated by 53 nucleotides Synpcc7942_0379 and Synpcc7942_0380 are separated by 21 nucleotides
Synpcc7942_0376: pqqE - putative zinc protease protein, at 370,341 to 371,867
pqqE
Synpcc7942_0377: rpoD4 - hypothetical protein, at 371,868 to 372,206
rpoD4
Synpcc7942_0378: ycf63 - Protein of unknown function DUF140, at 372,532 to 373,326
ycf63
Synpcc7942_0379: Synpcc7942_0379 - hypothetical protein, at 373,380 to 373,763
_0379
Synpcc7942_0380: Synpcc7942_0380 - hypothetical protein, at 373,785 to 374,828
_0380
Position (kb) 372
373
374 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 371.541 kb on - strand, within pqqE at 371.560 kb on - strand, within pqqE at 371.560 kb on - strand, within pqqE at 371.589 kb on - strand, within pqqE at 371.596 kb on + strand, within pqqE at 371.628 kb on + strand, within pqqE at 371.628 kb on + strand, within pqqE at 371.628 kb on + strand, within pqqE at 371.631 kb on - strand, within pqqE at 371.636 kb on - strand, within pqqE at 371.636 kb on - strand, within pqqE at 371.636 kb on - strand, within pqqE at 371.636 kb on - strand, within pqqE at 371.636 kb on - strand, within pqqE at 371.636 kb on - strand, within pqqE at 371.638 kb on + strand, within pqqE at 371.638 kb on - strand, within pqqE at 371.643 kb on + strand, within pqqE at 371.663 kb on + strand, within pqqE at 371.678 kb on + strand, within pqqE at 371.730 kb on - strand at 371.730 kb on - strand at 371.730 kb on - strand at 371.808 kb on - strand at 371.826 kb on - strand at 371.831 kb on - strand at 371.846 kb on + strand at 371.847 kb on + strand at 371.847 kb on + strand at 371.847 kb on + strand at 371.855 kb on - strand at 371.894 kb on + strand at 371.895 kb on + strand at 371.902 kb on - strand, within rpoD4 at 371.902 kb on - strand, within rpoD4 at 371.916 kb on + strand, within rpoD4 at 371.973 kb on + strand, within rpoD4 at 371.980 kb on - strand, within rpoD4 at 371.992 kb on - strand, within rpoD4 at 372.017 kb on + strand, within rpoD4 at 372.054 kb on + strand, within rpoD4 at 372.064 kb on + strand, within rpoD4 at 372.072 kb on - strand, within rpoD4 at 372.159 kb on - strand, within rpoD4 at 372.177 kb on + strand at 372.209 kb on - strand at 372.290 kb on + strand at 372.353 kb on + strand at 372.360 kb on + strand at 372.363 kb on - strand at 372.385 kb on + strand at 372.393 kb on - strand at 372.395 kb on + strand at 372.395 kb on + strand at 372.409 kb on + strand at 372.409 kb on + strand at 372.409 kb on + strand at 372.484 kb on + strand at 372.489 kb on + strand at 372.492 kb on - strand at 372.497 kb on - strand at 372.511 kb on + strand at 372.516 kb on - strand at 372.520 kb on - strand at 372.534 kb on + strand at 372.544 kb on + strand at 372.544 kb on + strand at 372.552 kb on - strand at 372.552 kb on - strand at 372.552 kb on - strand at 372.562 kb on - strand at 372.642 kb on + strand, within ycf63 at 372.642 kb on + strand, within ycf63 at 372.676 kb on - strand, within ycf63 at 372.680 kb on + strand, within ycf63 at 372.687 kb on + strand, within ycf63 at 372.695 kb on - strand, within ycf63 at 372.707 kb on - strand, within ycf63 at 372.708 kb on - strand, within ycf63 at 372.717 kb on - strand, within ycf63 at 372.732 kb on - strand, within ycf63 at 372.796 kb on - strand, within ycf63 at 372.802 kb on + strand, within ycf63 at 372.876 kb on + strand, within ycf63 at 372.913 kb on + strand, within ycf63 at 372.919 kb on + strand, within ycf63 at 372.927 kb on + strand, within ycf63 at 372.931 kb on + strand, within ycf63 at 372.950 kb on - strand, within ycf63 at 372.971 kb on + strand, within ycf63 at 372.995 kb on + strand, within ycf63 at 372.995 kb on + strand, within ycf63 at 373.003 kb on - strand, within ycf63 at 373.003 kb on - strand, within ycf63 at 373.019 kb on - strand, within ycf63 at 373.119 kb on - strand, within ycf63 at 373.120 kb on + strand, within ycf63 at 373.125 kb on + strand, within ycf63 at 373.133 kb on - strand, within ycf63 at 373.179 kb on - strand, within ycf63 at 373.196 kb on + strand, within ycf63 at 373.203 kb on + strand, within ycf63 at 373.231 kb on + strand, within ycf63 at 373.231 kb on - strand, within ycf63 at 373.271 kb on - strand at 373.292 kb on - strand at 373.320 kb on + strand at 373.328 kb on - strand at 373.329 kb on - strand at 373.329 kb on - strand at 373.333 kb on - strand at 373.346 kb on + strand at 373.354 kb on - strand at 373.356 kb on + strand at 373.415 kb on + strand at 373.418 kb on - strand at 373.423 kb on - strand, within Synpcc7942_0379 at 373.440 kb on - strand, within Synpcc7942_0379 at 373.502 kb on - strand, within Synpcc7942_0379 at 373.502 kb on - strand, within Synpcc7942_0379 at 373.502 kb on - strand, within Synpcc7942_0379 at 373.502 kb on - strand, within Synpcc7942_0379 at 373.519 kb on - strand, within Synpcc7942_0379 at 373.539 kb on - strand, within Synpcc7942_0379 at 373.539 kb on - strand, within Synpcc7942_0379 at 373.561 kb on - strand, within Synpcc7942_0379 at 373.610 kb on + strand, within Synpcc7942_0379 at 373.657 kb on - strand, within Synpcc7942_0379 at 373.715 kb on - strand, within Synpcc7942_0379 at 373.725 kb on - strand at 373.752 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0009765625 mg/ml remove 371,541 - pqqE Synpcc7942_0376 0.79 +0.2 371,560 - pqqE Synpcc7942_0376 0.80 -0.1 371,560 - pqqE Synpcc7942_0376 0.80 +0.3 371,589 - pqqE Synpcc7942_0376 0.82 +3.2 371,596 + pqqE Synpcc7942_0376 0.82 +0.9 371,628 + pqqE Synpcc7942_0376 0.84 +2.2 371,628 + pqqE Synpcc7942_0376 0.84 +0.5 371,628 + pqqE Synpcc7942_0376 0.84 -1.0 371,631 - pqqE Synpcc7942_0376 0.84 +0.9 371,636 - pqqE Synpcc7942_0376 0.85 +1.9 371,636 - pqqE Synpcc7942_0376 0.85 +0.5 371,636 - pqqE Synpcc7942_0376 0.85 +0.9 371,636 - pqqE Synpcc7942_0376 0.85 -0.1 371,636 - pqqE Synpcc7942_0376 0.85 -0.1 371,636 - pqqE Synpcc7942_0376 0.85 -0.1 371,638 + pqqE Synpcc7942_0376 0.85 -0.7 371,638 - pqqE Synpcc7942_0376 0.85 +0.9 371,643 + pqqE Synpcc7942_0376 0.85 -1.7 371,663 + pqqE Synpcc7942_0376 0.87 -0.5 371,678 + pqqE Synpcc7942_0376 0.88 -0.1 371,730 - -0.4 371,730 - +2.6 371,730 - +1.1 371,808 - +0.6 371,826 - +0.3 371,831 - +0.5 371,846 + -0.1 371,847 + -1.1 371,847 + -1.1 371,847 + -1.4 371,855 - -0.1 371,894 + -1.5 371,895 + +0.3 371,902 - rpoD4 Synpcc7942_0377 0.10 -2.9 371,902 - rpoD4 Synpcc7942_0377 0.10 -1.1 371,916 + rpoD4 Synpcc7942_0377 0.14 -0.3 371,973 + rpoD4 Synpcc7942_0377 0.31 -0.1 371,980 - rpoD4 Synpcc7942_0377 0.33 -1.7 371,992 - rpoD4 Synpcc7942_0377 0.37 +0.1 372,017 + rpoD4 Synpcc7942_0377 0.44 -0.2 372,054 + rpoD4 Synpcc7942_0377 0.55 +0.5 372,064 + rpoD4 Synpcc7942_0377 0.58 -0.3 372,072 - rpoD4 Synpcc7942_0377 0.60 -1.1 372,159 - rpoD4 Synpcc7942_0377 0.86 +3.0 372,177 + +2.1 372,209 - -2.4 372,290 + +1.6 372,353 + +0.3 372,360 + +0.3 372,363 - +1.9 372,385 + +0.2 372,393 - +0.1 372,395 + +2.4 372,395 + +0.5 372,409 + +2.5 372,409 + -0.1 372,409 + -0.1 372,484 + +0.9 372,489 + +0.5 372,492 - +0.5 372,497 - -0.5 372,511 + +0.7 372,516 - -1.1 372,520 - +1.5 372,534 + -2.4 372,544 + -0.5 372,544 + -0.7 372,552 - -0.7 372,552 - -0.4 372,552 - -1.1 372,562 - +0.2 372,642 + ycf63 Synpcc7942_0378 0.14 -1.1 372,642 + ycf63 Synpcc7942_0378 0.14 +0.5 372,676 - ycf63 Synpcc7942_0378 0.18 -0.1 372,680 + ycf63 Synpcc7942_0378 0.19 -2.4 372,687 + ycf63 Synpcc7942_0378 0.19 -0.1 372,695 - ycf63 Synpcc7942_0378 0.21 -2.1 372,707 - ycf63 Synpcc7942_0378 0.22 -1.9 372,708 - ycf63 Synpcc7942_0378 0.22 -2.4 372,717 - ycf63 Synpcc7942_0378 0.23 +0.5 372,732 - ycf63 Synpcc7942_0378 0.25 -2.9 372,796 - ycf63 Synpcc7942_0378 0.33 +0.2 372,802 + ycf63 Synpcc7942_0378 0.34 +0.8 372,876 + ycf63 Synpcc7942_0378 0.43 -0.8 372,913 + ycf63 Synpcc7942_0378 0.48 -2.1 372,919 + ycf63 Synpcc7942_0378 0.49 -2.3 372,927 + ycf63 Synpcc7942_0378 0.50 -0.1 372,931 + ycf63 Synpcc7942_0378 0.50 -1.1 372,950 - ycf63 Synpcc7942_0378 0.53 -1.1 372,971 + ycf63 Synpcc7942_0378 0.55 +0.5 372,995 + ycf63 Synpcc7942_0378 0.58 -0.8 372,995 + ycf63 Synpcc7942_0378 0.58 -0.1 373,003 - ycf63 Synpcc7942_0378 0.59 -1.7 373,003 - ycf63 Synpcc7942_0378 0.59 -1.1 373,019 - ycf63 Synpcc7942_0378 0.61 +0.1 373,119 - ycf63 Synpcc7942_0378 0.74 +1.1 373,120 + ycf63 Synpcc7942_0378 0.74 -1.1 373,125 + ycf63 Synpcc7942_0378 0.75 -0.8 373,133 - ycf63 Synpcc7942_0378 0.76 -3.1 373,179 - ycf63 Synpcc7942_0378 0.81 -1.1 373,196 + ycf63 Synpcc7942_0378 0.84 -0.5 373,203 + ycf63 Synpcc7942_0378 0.84 -1.3 373,231 + ycf63 Synpcc7942_0378 0.88 -0.1 373,231 - ycf63 Synpcc7942_0378 0.88 -1.1 373,271 - +1.2 373,292 - +0.9 373,320 + +1.9 373,328 - -1.7 373,329 - +1.9 373,329 - -0.5 373,333 - -0.1 373,346 + +1.0 373,354 - +3.1 373,356 + +1.5 373,415 + +3.1 373,418 - +0.9 373,423 - Synpcc7942_0379 0.11 +1.7 373,440 - Synpcc7942_0379 0.16 +6.5 373,502 - Synpcc7942_0379 0.32 +1.4 373,502 - Synpcc7942_0379 0.32 -1.1 373,502 - Synpcc7942_0379 0.32 +1.7 373,502 - Synpcc7942_0379 0.32 +1.9 373,519 - Synpcc7942_0379 0.36 +4.8 373,539 - Synpcc7942_0379 0.41 +1.3 373,539 - Synpcc7942_0379 0.41 +1.7 373,561 - Synpcc7942_0379 0.47 +2.4 373,610 + Synpcc7942_0379 0.60 +1.5 373,657 - Synpcc7942_0379 0.72 -0.1 373,715 - Synpcc7942_0379 0.87 -2.4 373,725 - +1.6 373,752 - +2.1
Or see this region's nucleotide sequence