Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00016 and SMc00017 are separated by 154 nucleotides SMc00017 and SMc00018 overlap by 4 nucleotides SMc00018 and SMc00072 are separated by 210 nucleotides
SMc00016: SMc00016 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 998,733 to 999,785
SMc00016
SMc00017: SMc00017 - homoserine kinase, at 999,940 to 1,000,920
SMc00017
SMc00018: SMc00018 - ribonuclease H, at 1,000,917 to 1,001,378
SMc00018
SMc00072: SMc00072 - peroxiredoxin protein, at 1,001,589 to 1,002,074
SMc00072
Position (kb)
999
1000
1001 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 999.792 kb on + strand at 999.792 kb on + strand at 999.869 kb on + strand at 999.869 kb on + strand at 999.894 kb on - strand at 999.915 kb on + strand at 999.915 kb on + strand at 999.916 kb on - strand at 999.916 kb on - strand at 1000.018 kb on + strand at 1000.081 kb on + strand, within SMc00017 at 1000.083 kb on + strand, within SMc00017 at 1000.888 kb on + strand at 1001.376 kb on + strand at 1001.377 kb on - strand at 1001.756 kb on + strand, within SMc00072
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 999,792 + -0.1 999,792 + +3.0 999,869 + -0.3 999,869 + +1.1 999,894 - -2.6 999,915 + +0.3 999,915 + -1.3 999,916 - -2.2 999,916 - -2.8 1,000,018 + -0.9 1,000,081 + SMc00017 0.14 -2.6 1,000,083 + SMc00017 0.15 -2.0 1,000,888 + +3.8 1,001,376 + -1.6 1,001,377 - -0.7 1,001,756 + SMc00072 0.34 +0.4
Or see this region's nucleotide sequence