Strain Fitness in Sinorhizobium meliloti 1021 around SMa5013

Experiment: L-Asparagine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSMa2343 and SMa5012 are separated by 54 nucleotidesSMa5012 and SMa5013 overlap by 4 nucleotidesSMa5013 and SMa2347 are separated by 21 nucleotides SMa2343: SMa2343 - oxidoreductase, at 1,320,285 to 1,321,007 SMa2343 SMa5012: SMa5012 - hypothetical protein, at 1,321,062 to 1,321,589 SMa5012 SMa5013: SMa5013 - hypothetical protein, at 1,321,586 to 1,321,999 SMa5013 SMa2347: SMa2347 - hypothetical protein, at 1,322,021 to 1,322,701 SMa2347 Position (kb) 1321 1322Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1320.646 kb on + strand, within SMa2343at 1320.671 kb on + strand, within SMa2343at 1320.734 kb on - strand, within SMa2343at 1320.736 kb on - strand, within SMa2343at 1320.736 kb on - strand, within SMa2343at 1320.791 kb on + strand, within SMa2343at 1320.889 kb on - strand, within SMa2343at 1321.057 kb on - strandat 1321.160 kb on + strand, within SMa5012at 1321.194 kb on - strand, within SMa5012at 1321.194 kb on - strand, within SMa5012at 1321.311 kb on + strand, within SMa5012at 1321.397 kb on + strand, within SMa5012at 1321.398 kb on - strand, within SMa5012at 1321.398 kb on - strand, within SMa5012at 1321.398 kb on - strand, within SMa5012at 1321.398 kb on - strand, within SMa5012at 1321.476 kb on - strand, within SMa5012at 1321.489 kb on + strand, within SMa5012at 1321.490 kb on - strand, within SMa5012at 1321.636 kb on + strand, within SMa5013at 1321.678 kb on + strand, within SMa5013at 1321.710 kb on + strand, within SMa5013at 1321.760 kb on + strand, within SMa5013at 1321.760 kb on + strand, within SMa5013at 1321.790 kb on + strand, within SMa5013at 1321.790 kb on + strand, within SMa5013at 1321.791 kb on - strand, within SMa5013at 1321.791 kb on - strand, within SMa5013at 1321.823 kb on + strand, within SMa5013at 1321.824 kb on - strand, within SMa5013at 1321.825 kb on + strand, within SMa5013at 1321.856 kb on + strand, within SMa5013at 1321.856 kb on + strand, within SMa5013at 1321.961 kb on - strandat 1322.054 kb on - strandat 1322.062 kb on - strandat 1322.245 kb on + strand, within SMa2347at 1322.245 kb on + strand, within SMa2347at 1322.246 kb on - strand, within SMa2347at 1322.388 kb on - strand, within SMa2347at 1322.391 kb on + strand, within SMa2347at 1322.392 kb on - strand, within SMa2347at 1322.647 kb on - strandat 1322.647 kb on - strandat 1322.732 kb on + strandat 1322.816 kb on - strandat 1322.862 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Asparagine (N)
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1,320,646 + SMa2343 0.50 +1.0
1,320,671 + SMa2343 0.53 -1.5
1,320,734 - SMa2343 0.62 -0.4
1,320,736 - SMa2343 0.62 +0.9
1,320,736 - SMa2343 0.62 +0.3
1,320,791 + SMa2343 0.70 -0.1
1,320,889 - SMa2343 0.84 +3.8
1,321,057 - -0.6
1,321,160 + SMa5012 0.19 +1.2
1,321,194 - SMa5012 0.25 +2.1
1,321,194 - SMa5012 0.25 +2.0
1,321,311 + SMa5012 0.47 +0.7
1,321,397 + SMa5012 0.63 +0.4
1,321,398 - SMa5012 0.64 +0.2
1,321,398 - SMa5012 0.64 -1.2
1,321,398 - SMa5012 0.64 -1.5
1,321,398 - SMa5012 0.64 -0.5
1,321,476 - SMa5012 0.78 +3.1
1,321,489 + SMa5012 0.81 -1.9
1,321,490 - SMa5012 0.81 +0.8
1,321,636 + SMa5013 0.12 +0.3
1,321,678 + SMa5013 0.22 +1.1
1,321,710 + SMa5013 0.30 -0.5
1,321,760 + SMa5013 0.42 +1.5
1,321,760 + SMa5013 0.42 +0.2
1,321,790 + SMa5013 0.49 +0.3
1,321,790 + SMa5013 0.49 +2.2
1,321,791 - SMa5013 0.50 -2.0
1,321,791 - SMa5013 0.50 +1.1
1,321,823 + SMa5013 0.57 -1.9
1,321,824 - SMa5013 0.57 -2.5
1,321,825 + SMa5013 0.58 -0.3
1,321,856 + SMa5013 0.65 -0.8
1,321,856 + SMa5013 0.65 +0.7
1,321,961 - -2.9
1,322,054 - +1.3
1,322,062 - -1.4
1,322,245 + SMa2347 0.33 -1.3
1,322,245 + SMa2347 0.33 -0.1
1,322,246 - SMa2347 0.33 -3.6
1,322,388 - SMa2347 0.54 -0.4
1,322,391 + SMa2347 0.54 -0.0
1,322,392 - SMa2347 0.54 -1.5
1,322,647 - -1.4
1,322,647 - +0.9
1,322,732 + -3.5
1,322,816 - +0.2
1,322,862 - -1.0

Or see this region's nucleotide sequence