Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0400 and SMa0402 are separated by 62 nucleotides SMa0402 and SMa0403 overlap by 53 nucleotides SMa0403 and SMa0404 are separated by 1 nucleotides
SMa0400: SMa0400 - Dehydrogenase, Zn-dependent, at 216,795 to 217,808
SMa0400
SMa0402: SMa0402 - GntR family transcriptional regulator, at 217,871 to 218,602
SMa0402
SMa0403: SMa0403 - hypothetical protein, at 218,550 to 219,938
SMa0403
SMa0404: SMa0404 - FMN-dependent dehydrogenase, at 219,940 to 221,085
SMa0404
Position (kb) 218
219
220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 217.578 kb on - strand, within SMa0400 at 217.746 kb on + strand at 217.877 kb on - strand at 217.877 kb on - strand at 217.914 kb on + strand at 217.914 kb on + strand at 217.915 kb on - strand at 218.161 kb on + strand, within SMa0402 at 218.316 kb on - strand, within SMa0402 at 218.376 kb on - strand, within SMa0402 at 218.376 kb on - strand, within SMa0402 at 218.381 kb on + strand, within SMa0402 at 218.381 kb on + strand, within SMa0402 at 218.549 kb on + strand at 218.549 kb on + strand at 218.550 kb on - strand at 218.550 kb on - strand at 218.550 kb on - strand at 218.614 kb on - strand at 218.614 kb on - strand at 218.614 kb on - strand at 218.678 kb on - strand at 218.787 kb on + strand, within SMa0403 at 218.787 kb on + strand, within SMa0403 at 218.788 kb on - strand, within SMa0403 at 218.886 kb on + strand, within SMa0403 at 218.887 kb on - strand, within SMa0403 at 218.889 kb on + strand, within SMa0403 at 218.889 kb on + strand, within SMa0403 at 219.102 kb on + strand, within SMa0403 at 219.103 kb on - strand, within SMa0403 at 219.119 kb on + strand, within SMa0403 at 219.119 kb on + strand, within SMa0403 at 219.120 kb on - strand, within SMa0403 at 219.181 kb on + strand, within SMa0403 at 219.182 kb on - strand, within SMa0403 at 219.228 kb on + strand, within SMa0403 at 219.228 kb on + strand, within SMa0403 at 219.229 kb on - strand, within SMa0403 at 219.316 kb on - strand, within SMa0403 at 219.562 kb on - strand, within SMa0403 at 219.562 kb on - strand, within SMa0403 at 219.588 kb on + strand, within SMa0403 at 219.676 kb on + strand, within SMa0403 at 219.842 kb on - strand at 219.842 kb on - strand at 219.896 kb on - strand at 220.009 kb on + strand at 220.010 kb on - strand at 220.012 kb on - strand at 220.074 kb on - strand, within SMa0404 at 220.129 kb on + strand, within SMa0404 at 220.254 kb on - strand, within SMa0404 at 220.324 kb on + strand, within SMa0404 at 220.325 kb on - strand, within SMa0404 at 220.362 kb on - strand, within SMa0404 at 220.376 kb on - strand, within SMa0404 at 220.419 kb on + strand, within SMa0404 at 220.848 kb on + strand, within SMa0404 at 220.849 kb on - strand, within SMa0404 at 220.849 kb on - strand, within SMa0404 at 220.933 kb on + strand, within SMa0404
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 217,578 - SMa0400 0.77 -2.0 217,746 + -0.7 217,877 - -2.4 217,877 - +0.6 217,914 + -0.2 217,914 + -2.1 217,915 - -0.9 218,161 + SMa0402 0.40 +0.3 218,316 - SMa0402 0.61 -2.8 218,376 - SMa0402 0.69 -2.2 218,376 - SMa0402 0.69 -0.0 218,381 + SMa0402 0.70 -1.1 218,381 + SMa0402 0.70 +0.7 218,549 + +0.1 218,549 + +0.5 218,550 - -0.1 218,550 - -0.4 218,550 - +0.6 218,614 - -0.3 218,614 - -2.4 218,614 - -1.1 218,678 - +0.3 218,787 + SMa0403 0.17 -0.6 218,787 + SMa0403 0.17 -2.9 218,788 - SMa0403 0.17 -0.1 218,886 + SMa0403 0.24 -0.4 218,887 - SMa0403 0.24 -2.9 218,889 + SMa0403 0.24 -2.8 218,889 + SMa0403 0.24 +1.9 219,102 + SMa0403 0.40 -0.4 219,103 - SMa0403 0.40 +0.5 219,119 + SMa0403 0.41 +0.1 219,119 + SMa0403 0.41 +0.4 219,120 - SMa0403 0.41 -0.8 219,181 + SMa0403 0.45 +0.6 219,182 - SMa0403 0.46 +2.3 219,228 + SMa0403 0.49 -2.3 219,228 + SMa0403 0.49 -1.6 219,229 - SMa0403 0.49 -1.8 219,316 - SMa0403 0.55 -0.6 219,562 - SMa0403 0.73 -2.3 219,562 - SMa0403 0.73 -0.9 219,588 + SMa0403 0.75 -0.4 219,676 + SMa0403 0.81 -0.9 219,842 - -1.1 219,842 - -1.4 219,896 - +0.2 220,009 + -1.1 220,010 - +1.1 220,012 - +2.7 220,074 - SMa0404 0.12 +0.6 220,129 + SMa0404 0.16 +1.2 220,254 - SMa0404 0.27 +0.2 220,324 + SMa0404 0.34 -0.3 220,325 - SMa0404 0.34 +1.4 220,362 - SMa0404 0.37 -0.2 220,376 - SMa0404 0.38 -0.3 220,419 + SMa0404 0.42 +0.6 220,848 + SMa0404 0.79 +0.6 220,849 - SMa0404 0.79 -0.1 220,849 - SMa0404 0.79 +0.8 220,933 + SMa0404 0.87 +0.3
Or see this region's nucleotide sequence