Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0085 and SMa0087 are separated by 16 nucleotides SMa0087 and SMa0089 are separated by 282 nucleotides SMa0089 and SMa0091 are separated by 124 nucleotides
SMa0085: SMa0085 - dehydrogenase, at 45,099 to 46,052
SMa0085
SMa0087: SMa0087 - hypothetical protein, at 46,069 to 46,401
SMa0087
SMa0089: SMa0089 - hypothetical protein, at 46,684 to 47,100
SMa0089
SMa0091: SMa0091 - hypothetical protein, at 47,225 to 47,920
SMa0091
Position (kb)
46
47
48 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 45.786 kb on - strand, within SMa0085 at 45.794 kb on - strand, within SMa0085 at 45.929 kb on + strand, within SMa0085 at 46.259 kb on - strand, within SMa0087 at 46.259 kb on - strand, within SMa0087 at 46.259 kb on - strand, within SMa0087 at 46.451 kb on - strand at 46.567 kb on + strand at 46.572 kb on - strand at 46.576 kb on + strand at 46.880 kb on + strand, within SMa0089 at 46.881 kb on - strand, within SMa0089 at 46.920 kb on + strand, within SMa0089 at 46.920 kb on + strand, within SMa0089 at 46.920 kb on + strand, within SMa0089 at 46.921 kb on - strand, within SMa0089 at 46.922 kb on + strand, within SMa0089 at 46.923 kb on - strand, within SMa0089 at 46.923 kb on - strand, within SMa0089 at 47.099 kb on - strand at 47.223 kb on - strand at 47.625 kb on - strand, within SMa0091 at 47.646 kb on - strand, within SMa0091 at 47.687 kb on + strand, within SMa0091 at 47.688 kb on - strand, within SMa0091 at 47.688 kb on - strand, within SMa0091 at 47.740 kb on + strand, within SMa0091 at 47.784 kb on - strand, within SMa0091 at 47.834 kb on + strand, within SMa0091 at 47.834 kb on + strand, within SMa0091 at 47.879 kb on + strand at 47.880 kb on - strand at 47.889 kb on - strand at 47.993 kb on + strand at 47.993 kb on + strand at 48.039 kb on + strand at 48.040 kb on - strand at 48.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 45,786 - SMa0085 0.72 -0.8 45,794 - SMa0085 0.73 -1.7 45,929 + SMa0085 0.87 +0.8 46,259 - SMa0087 0.57 +0.4 46,259 - SMa0087 0.57 +0.6 46,259 - SMa0087 0.57 +0.9 46,451 - -4.8 46,567 + +0.4 46,572 - -3.4 46,576 + -1.5 46,880 + SMa0089 0.47 -3.0 46,881 - SMa0089 0.47 -0.9 46,920 + SMa0089 0.57 -1.2 46,920 + SMa0089 0.57 -1.3 46,920 + SMa0089 0.57 +0.4 46,921 - SMa0089 0.57 -1.8 46,922 + SMa0089 0.57 -1.3 46,923 - SMa0089 0.57 -1.4 46,923 - SMa0089 0.57 -1.0 47,099 - -4.7 47,223 - -1.6 47,625 - SMa0091 0.57 +2.1 47,646 - SMa0091 0.60 -0.4 47,687 + SMa0091 0.66 -2.6 47,688 - SMa0091 0.67 -1.7 47,688 - SMa0091 0.67 +1.9 47,740 + SMa0091 0.74 -0.9 47,784 - SMa0091 0.80 -0.6 47,834 + SMa0091 0.88 -0.8 47,834 + SMa0091 0.88 -0.2 47,879 + -2.2 47,880 - -1.0 47,889 - -0.3 47,993 + +0.4 47,993 + -2.3 48,039 + +2.0 48,040 - -3.7 48,040 - -0.9
Or see this region's nucleotide sequence