Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20402 and SM_b20403 are separated by 2 nucleotides SM_b20403 and SM_b20404 overlap by 1 nucleotides SM_b20404 and SM_b20405 are separated by 12 nucleotides SM_b20405 and SM_b20406 are separated by 127 nucleotides
SM_b20402: SM_b20402 - alcohol dehydrogenase cytochrome c subunit precursor, at 414,971 to 416,359
_b20402
SM_b20403: SM_b20403 - oxidoreductase subunit protein, at 416,362 to 416,859
_b20403
SM_b20404: SM_b20404 - aldehyde dehydrogenase, at 416,859 to 419,099
_b20404
SM_b20405: SM_b20405 - transcriptional regulator, at 419,112 to 419,891
_b20405
SM_b20406: SM_b20406 - 5-oxoprolinase, at 420,019 to 422,133
_b20406
Position (kb) 416
417
418
419
420 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 415.943 kb on - strand, within SM_b20402 at 415.995 kb on - strand, within SM_b20402 at 416.210 kb on + strand, within SM_b20402 at 416.210 kb on + strand, within SM_b20402 at 416.210 kb on + strand, within SM_b20402 at 416.210 kb on + strand, within SM_b20402 at 416.210 kb on + strand, within SM_b20402 at 416.210 kb on + strand, within SM_b20402 at 416.211 kb on - strand, within SM_b20402 at 416.211 kb on - strand, within SM_b20402 at 416.211 kb on - strand, within SM_b20402 at 416.288 kb on + strand at 416.288 kb on + strand at 416.288 kb on + strand at 416.289 kb on - strand at 416.429 kb on - strand, within SM_b20403 at 416.429 kb on - strand, within SM_b20403 at 416.657 kb on - strand, within SM_b20403 at 416.773 kb on - strand, within SM_b20403 at 417.406 kb on + strand, within SM_b20404 at 417.444 kb on - strand, within SM_b20404 at 417.514 kb on - strand, within SM_b20404 at 417.514 kb on - strand, within SM_b20404 at 417.514 kb on - strand, within SM_b20404 at 417.709 kb on + strand, within SM_b20404 at 417.710 kb on - strand, within SM_b20404 at 417.710 kb on - strand, within SM_b20404 at 417.868 kb on - strand, within SM_b20404 at 417.874 kb on + strand, within SM_b20404 at 417.875 kb on - strand, within SM_b20404 at 418.045 kb on - strand, within SM_b20404 at 418.162 kb on - strand, within SM_b20404 at 418.162 kb on - strand, within SM_b20404 at 418.320 kb on - strand, within SM_b20404 at 418.320 kb on - strand, within SM_b20404 at 418.320 kb on - strand, within SM_b20404 at 418.365 kb on - strand, within SM_b20404 at 418.384 kb on + strand, within SM_b20404 at 418.384 kb on + strand, within SM_b20404 at 418.385 kb on - strand, within SM_b20404 at 418.385 kb on - strand, within SM_b20404 at 418.464 kb on + strand, within SM_b20404 at 418.662 kb on - strand, within SM_b20404 at 418.837 kb on - strand, within SM_b20404 at 419.060 kb on + strand at 419.182 kb on + strand at 419.183 kb on - strand at 419.183 kb on - strand at 419.183 kb on - strand at 419.530 kb on - strand, within SM_b20405 at 419.581 kb on + strand, within SM_b20405 at 419.581 kb on + strand, within SM_b20405 at 419.676 kb on + strand, within SM_b20405 at 419.677 kb on - strand, within SM_b20405 at 419.680 kb on + strand, within SM_b20405 at 419.680 kb on + strand, within SM_b20405 at 419.680 kb on + strand, within SM_b20405 at 419.680 kb on + strand, within SM_b20405 at 419.681 kb on - strand, within SM_b20405 at 419.683 kb on + strand, within SM_b20405 at 419.683 kb on + strand, within SM_b20405 at 419.684 kb on - strand, within SM_b20405 at 419.684 kb on - strand, within SM_b20405 at 419.687 kb on + strand, within SM_b20405 at 419.687 kb on + strand, within SM_b20405 at 419.688 kb on - strand, within SM_b20405 at 419.762 kb on - strand, within SM_b20405 at 419.808 kb on - strand, within SM_b20405 at 419.901 kb on + strand at 419.901 kb on + strand at 419.966 kb on + strand at 419.966 kb on + strand at 419.966 kb on + strand at 419.966 kb on + strand at 419.967 kb on - strand at 419.967 kb on - strand at 419.997 kb on + strand at 419.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 415,943 - SM_b20402 0.70 -1.1 415,995 - SM_b20402 0.74 -0.6 416,210 + SM_b20402 0.89 +0.7 416,210 + SM_b20402 0.89 -0.2 416,210 + SM_b20402 0.89 -1.6 416,210 + SM_b20402 0.89 -2.3 416,210 + SM_b20402 0.89 +0.0 416,210 + SM_b20402 0.89 +0.3 416,211 - SM_b20402 0.89 +3.3 416,211 - SM_b20402 0.89 -0.9 416,211 - SM_b20402 0.89 +0.5 416,288 + +0.2 416,288 + -0.2 416,288 + -1.0 416,289 - -0.2 416,429 - SM_b20403 0.13 +1.2 416,429 - SM_b20403 0.13 +0.0 416,657 - SM_b20403 0.59 +0.5 416,773 - SM_b20403 0.83 -1.1 417,406 + SM_b20404 0.24 +1.3 417,444 - SM_b20404 0.26 -1.9 417,514 - SM_b20404 0.29 -2.6 417,514 - SM_b20404 0.29 -0.1 417,514 - SM_b20404 0.29 -1.0 417,709 + SM_b20404 0.38 -0.2 417,710 - SM_b20404 0.38 -0.8 417,710 - SM_b20404 0.38 +0.0 417,868 - SM_b20404 0.45 +0.7 417,874 + SM_b20404 0.45 -2.2 417,875 - SM_b20404 0.45 -0.7 418,045 - SM_b20404 0.53 -1.7 418,162 - SM_b20404 0.58 -0.4 418,162 - SM_b20404 0.58 -0.2 418,320 - SM_b20404 0.65 -0.4 418,320 - SM_b20404 0.65 +1.5 418,320 - SM_b20404 0.65 -0.6 418,365 - SM_b20404 0.67 -0.5 418,384 + SM_b20404 0.68 +0.2 418,384 + SM_b20404 0.68 -0.7 418,385 - SM_b20404 0.68 -1.2 418,385 - SM_b20404 0.68 +1.4 418,464 + SM_b20404 0.72 +0.3 418,662 - SM_b20404 0.80 -1.8 418,837 - SM_b20404 0.88 -0.5 419,060 + +0.6 419,182 + +0.8 419,183 - +1.6 419,183 - +1.1 419,183 - -0.6 419,530 - SM_b20405 0.54 +0.6 419,581 + SM_b20405 0.60 +0.3 419,581 + SM_b20405 0.60 -0.6 419,676 + SM_b20405 0.72 +1.5 419,677 - SM_b20405 0.72 +1.1 419,680 + SM_b20405 0.73 -3.9 419,680 + SM_b20405 0.73 +0.7 419,680 + SM_b20405 0.73 +1.0 419,680 + SM_b20405 0.73 +3.6 419,681 - SM_b20405 0.73 -4.1 419,683 + SM_b20405 0.73 -2.4 419,683 + SM_b20405 0.73 +0.4 419,684 - SM_b20405 0.73 +0.4 419,684 - SM_b20405 0.73 -3.7 419,687 + SM_b20405 0.74 -3.0 419,687 + SM_b20405 0.74 +1.2 419,688 - SM_b20405 0.74 +0.3 419,762 - SM_b20405 0.83 +2.8 419,808 - SM_b20405 0.89 +1.1 419,901 + -0.1 419,901 + -0.0 419,966 + -1.4 419,966 + -0.5 419,966 + +0.2 419,966 + +2.9 419,967 - +0.9 419,967 - +0.0 419,997 + +0.4 419,998 - +0.1
Or see this region's nucleotide sequence