Experiment: Recovery broth with Erythromycin 7e-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12355 and BBR_RS12360 are separated by 170 nucleotides BBR_RS12360 and BBR_RS12365 are separated by 89 nucleotides BBR_RS12365 and BBR_RS12370 are separated by 115 nucleotides BBR_RS12370 and BBR_RS12375 are separated by 5 nucleotides
BBR_RS12355: BBR_RS12355 - ribonuclease III, at 524,160 to 524,903
_RS12355
BBR_RS12360: BBR_RS12360 - 50S ribosomal protein L32, at 525,074 to 525,268
_RS12360
BBR_RS12365: BBR_RS12365 - DUF177 domain-containing protein, at 525,358 to 525,978
_RS12365
BBR_RS12370: BBR_RS12370 - cell division protein, at 526,094 to 526,879
_RS12370
BBR_RS12375: BBR_RS12375 - pantetheine-phosphate adenylyltransferase, at 526,885 to 527,385
_RS12375
Position (kb)
525
526 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 524.465 kb on + strand, within BBR_RS12355 at 524.468 kb on - strand, within BBR_RS12355 at 524.552 kb on - strand, within BBR_RS12355 at 524.554 kb on + strand, within BBR_RS12355 at 524.621 kb on - strand, within BBR_RS12355 at 524.654 kb on - strand, within BBR_RS12355 at 524.701 kb on - strand, within BBR_RS12355 at 524.789 kb on + strand, within BBR_RS12355 at 524.810 kb on + strand, within BBR_RS12355 at 524.836 kb on + strand at 524.838 kb on + strand at 524.932 kb on - strand at 524.936 kb on + strand at 524.943 kb on + strand at 524.944 kb on - strand at 524.944 kb on - strand at 524.948 kb on + strand at 524.948 kb on + strand at 524.963 kb on - strand at 524.973 kb on - strand at 524.975 kb on - strand at 524.977 kb on + strand at 524.982 kb on - strand at 524.983 kb on + strand at 525.379 kb on + strand at 525.382 kb on - strand at 525.388 kb on - strand at 525.394 kb on - strand at 525.456 kb on + strand, within BBR_RS12365 at 525.456 kb on + strand, within BBR_RS12365 at 525.458 kb on - strand, within BBR_RS12365 at 525.461 kb on + strand, within BBR_RS12365 at 525.464 kb on - strand, within BBR_RS12365 at 525.475 kb on + strand, within BBR_RS12365 at 525.475 kb on + strand, within BBR_RS12365 at 525.476 kb on - strand, within BBR_RS12365 at 525.478 kb on - strand, within BBR_RS12365 at 525.480 kb on + strand, within BBR_RS12365 at 525.488 kb on - strand, within BBR_RS12365 at 525.488 kb on - strand, within BBR_RS12365 at 525.502 kb on - strand, within BBR_RS12365 at 525.528 kb on - strand, within BBR_RS12365 at 525.529 kb on - strand, within BBR_RS12365 at 525.533 kb on - strand, within BBR_RS12365 at 525.602 kb on - strand, within BBR_RS12365 at 525.612 kb on - strand, within BBR_RS12365 at 525.662 kb on + strand, within BBR_RS12365 at 525.667 kb on + strand, within BBR_RS12365 at 525.675 kb on - strand, within BBR_RS12365 at 525.675 kb on - strand, within BBR_RS12365 at 525.676 kb on - strand, within BBR_RS12365 at 525.716 kb on + strand, within BBR_RS12365 at 525.721 kb on + strand, within BBR_RS12365 at 525.735 kb on + strand, within BBR_RS12365 at 525.743 kb on - strand, within BBR_RS12365 at 525.844 kb on - strand, within BBR_RS12365 at 525.850 kb on - strand, within BBR_RS12365 at 525.854 kb on - strand, within BBR_RS12365 at 525.862 kb on - strand, within BBR_RS12365 at 525.890 kb on - strand, within BBR_RS12365 at 525.896 kb on + strand, within BBR_RS12365 at 525.905 kb on + strand, within BBR_RS12365 at 525.939 kb on - strand at 526.027 kb on - strand at 526.034 kb on + strand at 526.065 kb on + strand at 526.070 kb on + strand at 526.073 kb on - strand at 526.073 kb on - strand at 526.078 kb on - strand at 526.078 kb on - strand at 526.082 kb on + strand at 526.082 kb on + strand at 526.090 kb on - strand at 526.090 kb on - strand at 526.090 kb on - strand at 526.110 kb on - strand at 526.131 kb on + strand at 526.733 kb on - strand, within BBR_RS12370 at 526.891 kb on - strand at 526.896 kb on - strand at 526.898 kb on + strand at 526.916 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Erythromycin 7e-05 mM remove 524,465 + BBR_RS12355 0.41 -0.5 524,468 - BBR_RS12355 0.41 +0.6 524,552 - BBR_RS12355 0.53 +0.8 524,554 + BBR_RS12355 0.53 +1.4 524,621 - BBR_RS12355 0.62 -0.2 524,654 - BBR_RS12355 0.66 -0.1 524,701 - BBR_RS12355 0.73 +2.0 524,789 + BBR_RS12355 0.85 +1.6 524,810 + BBR_RS12355 0.87 -1.1 524,836 + +1.8 524,838 + -1.1 524,932 - +0.5 524,936 + +0.0 524,943 + +0.7 524,944 - -0.2 524,944 - -0.6 524,948 + -1.3 524,948 + +0.4 524,963 - -0.8 524,973 - -0.3 524,975 - +0.1 524,977 + -1.4 524,982 - +0.8 524,983 + -0.6 525,379 + -2.3 525,382 - -1.1 525,388 - -0.6 525,394 - -1.0 525,456 + BBR_RS12365 0.16 -1.9 525,456 + BBR_RS12365 0.16 -2.3 525,458 - BBR_RS12365 0.16 -2.3 525,461 + BBR_RS12365 0.17 -1.1 525,464 - BBR_RS12365 0.17 -1.9 525,475 + BBR_RS12365 0.19 -1.4 525,475 + BBR_RS12365 0.19 -0.8 525,476 - BBR_RS12365 0.19 -0.4 525,478 - BBR_RS12365 0.19 -2.6 525,480 + BBR_RS12365 0.20 +0.4 525,488 - BBR_RS12365 0.21 +0.8 525,488 - BBR_RS12365 0.21 -0.9 525,502 - BBR_RS12365 0.23 -2.1 525,528 - BBR_RS12365 0.27 -1.6 525,529 - BBR_RS12365 0.28 +0.1 525,533 - BBR_RS12365 0.28 -1.6 525,602 - BBR_RS12365 0.39 -1.9 525,612 - BBR_RS12365 0.41 -1.4 525,662 + BBR_RS12365 0.49 -2.7 525,667 + BBR_RS12365 0.50 -0.4 525,675 - BBR_RS12365 0.51 +0.1 525,675 - BBR_RS12365 0.51 +0.8 525,676 - BBR_RS12365 0.51 -0.9 525,716 + BBR_RS12365 0.58 -0.8 525,721 + BBR_RS12365 0.58 -1.1 525,735 + BBR_RS12365 0.61 -1.8 525,743 - BBR_RS12365 0.62 -1.7 525,844 - BBR_RS12365 0.78 -0.1 525,850 - BBR_RS12365 0.79 -0.7 525,854 - BBR_RS12365 0.80 -2.1 525,862 - BBR_RS12365 0.81 -2.7 525,890 - BBR_RS12365 0.86 -2.7 525,896 + BBR_RS12365 0.87 -1.4 525,905 + BBR_RS12365 0.88 -1.7 525,939 - -2.2 526,027 - +1.2 526,034 + -0.3 526,065 + -0.6 526,070 + -0.3 526,073 - +0.1 526,073 - -0.5 526,078 - +0.5 526,078 - -0.8 526,082 + +0.3 526,082 + +0.1 526,090 - +1.2 526,090 - +0.0 526,090 - +0.1 526,110 - -0.3 526,131 + -0.6 526,733 - BBR_RS12370 0.81 +0.8 526,891 - -0.6 526,896 - -0.0 526,898 + +0.8 526,916 + -0.1
Or see this region's nucleotide sequence