Experiment: Hexanoic 21 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1576 and Rru_A1577 are separated by 101 nucleotides Rru_A1577 and Rru_A1578 overlap by 4 nucleotides Rru_A1578 and Rru_A1579 overlap by 8 nucleotides Rru_A1579 and Rru_A1580 are separated by 21 nucleotides
Rru_A1576: Rru_A1576 - Prolyl-tRNA synthetase (NCBI), at 1,856,768 to 1,858,075
_A1576
Rru_A1577: Rru_A1577 - ThiJ/PfpI (NCBI), at 1,858,177 to 1,858,806
_A1577
Rru_A1578: Rru_A1578 - Lipoprotein releasing system, transmembrane protein, LolC/E family (NCBI), at 1,858,803 to 1,860,056
_A1578
Rru_A1579: Rru_A1579 - ABC transporter component (NCBI), at 1,860,049 to 1,860,807
_A1579
Rru_A1580: Rru_A1580 - GCN5-related N-acetyltransferase (NCBI), at 1,860,829 to 1,861,266
_A1580
Position (kb)
1858
1859
1860
1861 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1858.196 kb on + strand at 1858.198 kb on + strand at 1858.198 kb on + strand at 1858.198 kb on + strand at 1858.198 kb on + strand at 1860.810 kb on - strand at 1860.810 kb on - strand at 1860.810 kb on - strand at 1860.836 kb on + strand at 1860.836 kb on + strand at 1860.837 kb on - strand at 1860.906 kb on + strand, within Rru_A1580 at 1860.906 kb on + strand, within Rru_A1580 at 1860.906 kb on + strand, within Rru_A1580 at 1860.907 kb on - strand, within Rru_A1580
Per-strain Table
Position Strand Gene LocusTag Fraction Hexanoic 21 mM; anaerobic remove 1,858,196 + -0.5 1,858,198 + -0.5 1,858,198 + -1.4 1,858,198 + -0.7 1,858,198 + +0.3 1,860,810 - -1.9 1,860,810 - +2.0 1,860,810 - +1.1 1,860,836 + +1.7 1,860,836 + +0.2 1,860,837 - +0.0 1,860,906 + Rru_A1580 0.18 +0.5 1,860,906 + Rru_A1580 0.18 +0.3 1,860,906 + Rru_A1580 0.18 +1.3 1,860,907 - Rru_A1580 0.18 -2.6
Or see this region's nucleotide sequence