Experiment: PYE with Spectinomycin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00914 and CCNA_00915 are separated by 131 nucleotides CCNA_00915 and CCNA_00916 are separated by 0 nucleotides CCNA_00916 and CCNA_00917 overlap by 4 nucleotides CCNA_00917 and CCNA_00918 are separated by 73 nucleotides
CCNA_00914: CCNA_00914 - acetyltransferase, at 993,288 to 993,815
_00914
CCNA_00915: CCNA_00915 - Molybdenum cofactor sulfurase related protein, at 993,947 to 994,708
_00915
CCNA_00916: CCNA_00916 - toxin protein parE-1, at 994,709 to 994,999
_00916
CCNA_00917: CCNA_00917 - antitoxin protein parD-1, at 994,996 to 995,262
_00917
CCNA_00918: CCNA_00918 - peptide release factor-glutamine N5-methyltransferase, at 995,336 to 996,205
_00918
Position (kb)
994
995 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 993.718 kb on + strand, within CCNA_00914 at 993.723 kb on + strand, within CCNA_00914 at 993.723 kb on + strand, within CCNA_00914 at 993.723 kb on + strand, within CCNA_00914 at 993.723 kb on + strand, within CCNA_00914 at 993.723 kb on + strand, within CCNA_00914 at 993.723 kb on + strand, within CCNA_00914 at 993.725 kb on - strand, within CCNA_00914 at 993.731 kb on - strand, within CCNA_00914 at 993.754 kb on + strand, within CCNA_00914 at 993.762 kb on - strand, within CCNA_00914 at 993.762 kb on - strand, within CCNA_00914 at 993.764 kb on - strand at 993.783 kb on + strand at 993.783 kb on + strand at 993.790 kb on + strand at 993.790 kb on + strand at 993.790 kb on + strand at 993.791 kb on - strand at 993.798 kb on - strand at 993.798 kb on - strand at 993.821 kb on + strand at 993.821 kb on + strand at 993.829 kb on - strand at 993.829 kb on - strand at 993.831 kb on - strand at 993.839 kb on - strand at 993.839 kb on - strand at 993.897 kb on - strand at 993.937 kb on + strand at 993.991 kb on - strand at 993.999 kb on - strand at 994.054 kb on + strand, within CCNA_00915 at 994.054 kb on + strand, within CCNA_00915 at 994.054 kb on + strand, within CCNA_00915 at 994.057 kb on - strand, within CCNA_00915 at 994.062 kb on - strand, within CCNA_00915 at 994.130 kb on + strand, within CCNA_00915 at 994.135 kb on + strand, within CCNA_00915 at 994.138 kb on - strand, within CCNA_00915 at 994.143 kb on - strand, within CCNA_00915 at 994.169 kb on + strand, within CCNA_00915 at 994.188 kb on - strand, within CCNA_00915 at 994.306 kb on - strand, within CCNA_00915 at 994.370 kb on - strand, within CCNA_00915 at 994.397 kb on - strand, within CCNA_00915 at 994.411 kb on + strand, within CCNA_00915 at 994.419 kb on - strand, within CCNA_00915 at 994.419 kb on - strand, within CCNA_00915 at 994.450 kb on + strand, within CCNA_00915 at 994.450 kb on + strand, within CCNA_00915 at 994.450 kb on + strand, within CCNA_00915 at 994.450 kb on + strand, within CCNA_00915 at 994.450 kb on + strand, within CCNA_00915 at 994.450 kb on + strand, within CCNA_00915 at 994.451 kb on - strand, within CCNA_00915 at 994.458 kb on - strand, within CCNA_00915 at 994.458 kb on - strand, within CCNA_00915 at 994.458 kb on - strand, within CCNA_00915 at 994.458 kb on - strand, within CCNA_00915 at 994.458 kb on - strand, within CCNA_00915 at 994.490 kb on - strand, within CCNA_00915 at 994.505 kb on - strand, within CCNA_00915 at 994.540 kb on + strand, within CCNA_00915 at 994.542 kb on - strand, within CCNA_00915 at 994.542 kb on - strand, within CCNA_00915 at 994.571 kb on + strand, within CCNA_00915 at 994.571 kb on + strand, within CCNA_00915 at 994.603 kb on + strand, within CCNA_00915 at 994.603 kb on + strand, within CCNA_00915 at 994.603 kb on + strand, within CCNA_00915 at 994.611 kb on - strand, within CCNA_00915 at 994.611 kb on - strand, within CCNA_00915 at 994.629 kb on + strand, within CCNA_00915 at 994.636 kb on + strand at 994.637 kb on - strand at 994.646 kb on + strand at 994.649 kb on + strand at 994.666 kb on + strand at 994.718 kb on - strand at 994.730 kb on - strand at 994.766 kb on - strand, within CCNA_00916 at 994.781 kb on + strand, within CCNA_00916 at 994.789 kb on - strand, within CCNA_00916 at 994.789 kb on - strand, within CCNA_00916 at 994.814 kb on + strand, within CCNA_00916 at 994.814 kb on + strand, within CCNA_00916 at 994.822 kb on - strand, within CCNA_00916 at 994.822 kb on - strand, within CCNA_00916 at 994.822 kb on - strand, within CCNA_00916 at 994.849 kb on - strand, within CCNA_00916 at 994.915 kb on + strand, within CCNA_00916 at 994.918 kb on - strand, within CCNA_00916 at 994.923 kb on + strand, within CCNA_00916 at 994.933 kb on + strand, within CCNA_00916 at 994.933 kb on + strand, within CCNA_00916 at 994.933 kb on + strand, within CCNA_00916 at 994.936 kb on - strand, within CCNA_00916 at 994.941 kb on - strand, within CCNA_00916 at 994.941 kb on - strand, within CCNA_00916 at 994.941 kb on - strand, within CCNA_00916 at 994.944 kb on + strand, within CCNA_00916 at 994.944 kb on + strand, within CCNA_00916 at 994.952 kb on - strand, within CCNA_00916 at 994.952 kb on - strand, within CCNA_00916 at 994.952 kb on - strand, within CCNA_00916 at 994.952 kb on - strand, within CCNA_00916 at 994.952 kb on - strand, within CCNA_00916 at 994.987 kb on - strand at 995.027 kb on - strand, within CCNA_00917 at 995.028 kb on + strand, within CCNA_00917 at 995.028 kb on + strand, within CCNA_00917 at 995.028 kb on + strand, within CCNA_00917 at 995.028 kb on + strand, within CCNA_00917 at 995.028 kb on + strand, within CCNA_00917 at 995.028 kb on + strand, within CCNA_00917 at 995.035 kb on - strand, within CCNA_00917 at 995.035 kb on - strand, within CCNA_00917 at 995.036 kb on - strand, within CCNA_00917 at 995.036 kb on - strand, within CCNA_00917 at 995.036 kb on - strand, within CCNA_00917 at 995.036 kb on - strand, within CCNA_00917 at 995.036 kb on - strand, within CCNA_00917 at 995.036 kb on - strand, within CCNA_00917 at 995.036 kb on - strand, within CCNA_00917 at 995.070 kb on + strand, within CCNA_00917 at 995.113 kb on - strand, within CCNA_00917 at 995.146 kb on + strand, within CCNA_00917 at 995.146 kb on + strand, within CCNA_00917 at 995.180 kb on + strand, within CCNA_00917 at 995.339 kb on + strand at 995.339 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml remove 993,718 + CCNA_00914 0.81 -1.5 993,723 + CCNA_00914 0.82 -1.7 993,723 + CCNA_00914 0.82 +0.9 993,723 + CCNA_00914 0.82 -0.2 993,723 + CCNA_00914 0.82 +0.2 993,723 + CCNA_00914 0.82 -0.0 993,723 + CCNA_00914 0.82 +0.2 993,725 - CCNA_00914 0.83 -0.9 993,731 - CCNA_00914 0.84 -1.4 993,754 + CCNA_00914 0.88 +0.5 993,762 - CCNA_00914 0.90 -1.0 993,762 - CCNA_00914 0.90 -1.5 993,764 - -0.9 993,783 + -1.8 993,783 + +0.9 993,790 + +1.1 993,790 + +1.3 993,790 + -0.3 993,791 - +0.7 993,798 - +1.2 993,798 - +2.3 993,821 + -0.4 993,821 + -1.3 993,829 - +1.3 993,829 - -0.0 993,831 - +1.7 993,839 - +1.3 993,839 - +1.1 993,897 - +0.7 993,937 + -0.9 993,991 - +0.9 993,999 - -1.5 994,054 + CCNA_00915 0.14 +0.3 994,054 + CCNA_00915 0.14 -2.0 994,054 + CCNA_00915 0.14 -0.2 994,057 - CCNA_00915 0.14 +1.0 994,062 - CCNA_00915 0.15 -0.9 994,130 + CCNA_00915 0.24 +0.5 994,135 + CCNA_00915 0.25 -1.0 994,138 - CCNA_00915 0.25 -0.4 994,143 - CCNA_00915 0.26 -1.4 994,169 + CCNA_00915 0.29 +0.8 994,188 - CCNA_00915 0.32 -0.2 994,306 - CCNA_00915 0.47 -1.6 994,370 - CCNA_00915 0.56 +1.0 994,397 - CCNA_00915 0.59 +2.0 994,411 + CCNA_00915 0.61 -0.5 994,419 - CCNA_00915 0.62 +1.3 994,419 - CCNA_00915 0.62 +0.4 994,450 + CCNA_00915 0.66 -0.2 994,450 + CCNA_00915 0.66 +2.1 994,450 + CCNA_00915 0.66 +0.4 994,450 + CCNA_00915 0.66 -0.1 994,450 + CCNA_00915 0.66 -0.4 994,450 + CCNA_00915 0.66 +0.0 994,451 - CCNA_00915 0.66 -1.5 994,458 - CCNA_00915 0.67 +0.1 994,458 - CCNA_00915 0.67 -1.0 994,458 - CCNA_00915 0.67 +0.6 994,458 - CCNA_00915 0.67 -0.6 994,458 - CCNA_00915 0.67 -1.8 994,490 - CCNA_00915 0.71 +0.7 994,505 - CCNA_00915 0.73 +0.9 994,540 + CCNA_00915 0.78 +0.4 994,542 - CCNA_00915 0.78 +0.1 994,542 - CCNA_00915 0.78 +0.0 994,571 + CCNA_00915 0.82 -0.1 994,571 + CCNA_00915 0.82 +0.5 994,603 + CCNA_00915 0.86 +0.1 994,603 + CCNA_00915 0.86 -1.0 994,603 + CCNA_00915 0.86 +1.1 994,611 - CCNA_00915 0.87 +1.4 994,611 - CCNA_00915 0.87 -0.4 994,629 + CCNA_00915 0.90 -0.6 994,636 + -0.9 994,637 - -0.5 994,646 + -0.8 994,649 + -0.0 994,666 + +0.2 994,718 - +1.4 994,730 - -0.8 994,766 - CCNA_00916 0.20 -0.8 994,781 + CCNA_00916 0.25 +1.1 994,789 - CCNA_00916 0.27 -0.4 994,789 - CCNA_00916 0.27 +0.5 994,814 + CCNA_00916 0.36 -1.5 994,814 + CCNA_00916 0.36 -0.1 994,822 - CCNA_00916 0.39 -1.3 994,822 - CCNA_00916 0.39 -1.6 994,822 - CCNA_00916 0.39 -0.9 994,849 - CCNA_00916 0.48 -0.7 994,915 + CCNA_00916 0.71 -0.7 994,918 - CCNA_00916 0.72 +0.7 994,923 + CCNA_00916 0.74 -0.2 994,933 + CCNA_00916 0.77 -0.0 994,933 + CCNA_00916 0.77 +0.5 994,933 + CCNA_00916 0.77 -1.4 994,936 - CCNA_00916 0.78 -0.3 994,941 - CCNA_00916 0.80 -1.9 994,941 - CCNA_00916 0.80 +0.4 994,941 - CCNA_00916 0.80 -0.2 994,944 + CCNA_00916 0.81 -0.5 994,944 + CCNA_00916 0.81 -0.8 994,952 - CCNA_00916 0.84 -1.3 994,952 - CCNA_00916 0.84 -0.1 994,952 - CCNA_00916 0.84 -1.4 994,952 - CCNA_00916 0.84 -1.4 994,952 - CCNA_00916 0.84 +0.5 994,987 - +0.0 995,027 - CCNA_00917 0.12 +0.4 995,028 + CCNA_00917 0.12 +0.5 995,028 + CCNA_00917 0.12 -0.3 995,028 + CCNA_00917 0.12 -1.4 995,028 + CCNA_00917 0.12 -0.4 995,028 + CCNA_00917 0.12 -1.9 995,028 + CCNA_00917 0.12 -0.2 995,035 - CCNA_00917 0.15 +1.1 995,035 - CCNA_00917 0.15 +0.9 995,036 - CCNA_00917 0.15 -0.0 995,036 - CCNA_00917 0.15 +0.8 995,036 - CCNA_00917 0.15 +1.0 995,036 - CCNA_00917 0.15 +0.4 995,036 - CCNA_00917 0.15 -0.6 995,036 - CCNA_00917 0.15 -1.4 995,036 - CCNA_00917 0.15 +1.5 995,070 + CCNA_00917 0.28 +1.4 995,113 - CCNA_00917 0.44 +0.4 995,146 + CCNA_00917 0.56 +1.1 995,146 + CCNA_00917 0.56 -1.2 995,180 + CCNA_00917 0.69 +1.1 995,339 + +2.1 995,339 + +0.2
Or see this region's nucleotide sequence