Experiment: PYE with Spectinomycin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00525 and CCNA_00526 are separated by 114 nucleotides CCNA_00526 and CCNA_00527 are separated by 130 nucleotides CCNA_00527 and CCNA_00528 are separated by 180 nucleotides
CCNA_00525: CCNA_00525 - prolipoprotein diacylglyceryl transferase, at 538,746 to 539,654
_00525
CCNA_00526: CCNA_00526 - BMFP membrane fusogenic protein, at 539,769 to 540,026
_00526
CCNA_00527: CCNA_00527 - hypothetical protein, at 540,157 to 540,663
_00527
CCNA_00528: CCNA_00528 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) <small>(from data)</small>, at 540,844 to 541,626
_00528
Position (kb) 540
541 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 539.352 kb on - strand, within CCNA_00525 at 539.505 kb on - strand, within CCNA_00525 at 539.656 kb on + strand at 539.676 kb on - strand at 539.678 kb on + strand at 539.678 kb on + strand at 539.678 kb on + strand at 539.678 kb on + strand at 539.678 kb on + strand at 539.678 kb on + strand at 539.678 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.688 kb on + strand at 539.690 kb on + strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.696 kb on - strand at 539.697 kb on + strand at 539.709 kb on + strand at 539.714 kb on + strand at 539.766 kb on + strand at 539.904 kb on - strand, within CCNA_00526 at 540.045 kb on - strand at 540.066 kb on - strand at 540.082 kb on + strand at 540.090 kb on - strand at 540.091 kb on + strand at 540.091 kb on + strand at 540.091 kb on + strand at 540.116 kb on + strand at 540.116 kb on + strand at 540.116 kb on + strand at 540.116 kb on + strand at 540.124 kb on - strand at 540.124 kb on - strand at 540.124 kb on - strand at 540.143 kb on + strand at 540.179 kb on + strand at 540.186 kb on + strand at 540.203 kb on + strand at 540.203 kb on + strand at 540.203 kb on + strand at 540.245 kb on + strand, within CCNA_00527 at 540.253 kb on - strand, within CCNA_00527 at 540.253 kb on - strand, within CCNA_00527 at 540.263 kb on - strand, within CCNA_00527 at 540.268 kb on + strand, within CCNA_00527 at 540.335 kb on - strand, within CCNA_00527 at 540.343 kb on + strand, within CCNA_00527 at 540.346 kb on + strand, within CCNA_00527 at 540.349 kb on - strand, within CCNA_00527 at 540.349 kb on - strand, within CCNA_00527 at 540.363 kb on + strand, within CCNA_00527 at 540.398 kb on + strand, within CCNA_00527 at 540.406 kb on + strand, within CCNA_00527 at 540.406 kb on - strand, within CCNA_00527 at 540.450 kb on + strand, within CCNA_00527 at 540.467 kb on + strand, within CCNA_00527 at 540.486 kb on + strand, within CCNA_00527 at 540.487 kb on + strand, within CCNA_00527 at 540.488 kb on + strand, within CCNA_00527 at 540.488 kb on + strand, within CCNA_00527 at 540.498 kb on + strand, within CCNA_00527 at 540.498 kb on + strand, within CCNA_00527 at 540.498 kb on + strand, within CCNA_00527 at 540.498 kb on + strand, within CCNA_00527 at 540.498 kb on + strand, within CCNA_00527 at 540.511 kb on + strand, within CCNA_00527 at 540.519 kb on - strand, within CCNA_00527 at 540.522 kb on + strand, within CCNA_00527 at 540.530 kb on - strand, within CCNA_00527 at 540.588 kb on - strand, within CCNA_00527 at 540.609 kb on - strand, within CCNA_00527 at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.657 kb on + strand at 540.664 kb on + strand at 540.664 kb on + strand at 540.664 kb on + strand at 540.664 kb on + strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.672 kb on - strand at 540.718 kb on + strand at 540.718 kb on + strand at 540.751 kb on + strand at 540.751 kb on + strand at 540.751 kb on + strand at 540.751 kb on + strand at 540.751 kb on + strand at 540.751 kb on + strand at 540.754 kb on - strand at 540.759 kb on - strand at 540.759 kb on - strand at 540.759 kb on - strand at 540.759 kb on - strand at 540.776 kb on + strand at 540.820 kb on + strand at 540.827 kb on + strand at 540.827 kb on + strand at 540.879 kb on - strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.900 kb on - strand at 540.967 kb on + strand, within CCNA_00528 at 540.992 kb on - strand at 540.992 kb on - strand, within CCNA_00528 at 541.004 kb on - strand, within CCNA_00528 at 541.047 kb on + strand, within CCNA_00528 at 541.047 kb on + strand, within CCNA_00528 at 541.083 kb on - strand, within CCNA_00528 at 541.083 kb on - strand, within CCNA_00528 at 541.115 kb on + strand, within CCNA_00528 at 541.123 kb on - strand, within CCNA_00528 at 541.123 kb on - strand, within CCNA_00528 at 541.154 kb on - strand at 541.214 kb on - strand, within CCNA_00528 at 541.225 kb on - strand, within CCNA_00528 at 541.240 kb on + strand, within CCNA_00528 at 541.253 kb on + strand, within CCNA_00528 at 541.298 kb on + strand, within CCNA_00528 at 541.303 kb on + strand, within CCNA_00528 at 541.303 kb on - strand, within CCNA_00528 at 541.327 kb on + strand, within CCNA_00528 at 541.347 kb on - strand, within CCNA_00528 at 541.351 kb on + strand, within CCNA_00528 at 541.351 kb on + strand, within CCNA_00528 at 541.379 kb on - strand, within CCNA_00528 at 541.390 kb on + strand, within CCNA_00528 at 541.390 kb on + strand, within CCNA_00528 at 541.390 kb on + strand, within CCNA_00528 at 541.390 kb on + strand, within CCNA_00528 at 541.390 kb on + strand, within CCNA_00528 at 541.397 kb on + strand, within CCNA_00528 at 541.398 kb on - strand, within CCNA_00528 at 541.398 kb on - strand, within CCNA_00528 at 541.398 kb on - strand, within CCNA_00528 at 541.403 kb on + strand, within CCNA_00528 at 541.403 kb on + strand, within CCNA_00528 at 541.405 kb on - strand, within CCNA_00528 at 541.405 kb on - strand, within CCNA_00528 at 541.405 kb on - strand at 541.411 kb on - strand, within CCNA_00528 at 541.416 kb on - strand, within CCNA_00528 at 541.462 kb on + strand, within CCNA_00528 at 541.462 kb on + strand, within CCNA_00528 at 541.480 kb on - strand, within CCNA_00528 at 541.480 kb on - strand, within CCNA_00528 at 541.481 kb on + strand, within CCNA_00528 at 541.489 kb on - strand, within CCNA_00528 at 541.508 kb on - strand, within CCNA_00528 at 541.540 kb on + strand, within CCNA_00528 at 541.540 kb on + strand, within CCNA_00528 at 541.540 kb on + strand, within CCNA_00528 at 541.540 kb on + strand, within CCNA_00528 at 541.548 kb on - strand at 541.557 kb on - strand at 541.578 kb on - strand at 541.582 kb on + strand at 541.582 kb on + strand at 541.583 kb on - strand at 541.594 kb on + strand at 541.652 kb on + strand at 541.652 kb on + strand at 541.652 kb on + strand at 541.652 kb on + strand at 541.657 kb on + strand at 541.657 kb on + strand at 541.657 kb on + strand at 541.657 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml remove 539,352 - CCNA_00525 0.67 +1.5 539,505 - CCNA_00525 0.83 -0.4 539,656 + -1.4 539,676 - +0.1 539,678 + -0.8 539,678 + +0.3 539,678 + -0.3 539,678 + +1.4 539,678 + -1.1 539,678 + +0.8 539,678 + +0.4 539,688 + +1.4 539,688 + -1.5 539,688 + +0.9 539,688 + +0.1 539,688 + +0.1 539,688 + -0.2 539,688 + +1.5 539,688 + -0.6 539,688 + -0.2 539,688 + +1.4 539,688 + -0.6 539,688 + +0.1 539,688 + -2.4 539,688 + -1.2 539,688 + -0.9 539,688 + -0.8 539,688 + -1.7 539,688 + +0.3 539,688 + +0.3 539,688 + -2.3 539,690 + +0.7 539,696 - +1.6 539,696 - -1.0 539,696 - -0.5 539,696 - -0.5 539,696 - +0.1 539,696 - -1.4 539,696 - -1.7 539,696 - -0.7 539,696 - +1.5 539,696 - -0.1 539,696 - -0.4 539,696 - -1.6 539,696 - +0.3 539,696 - -0.5 539,696 - -0.1 539,696 - +0.1 539,696 - +0.8 539,696 - +0.4 539,696 - -0.9 539,696 - -2.1 539,696 - -0.9 539,696 - -1.5 539,696 - +1.2 539,696 - -1.1 539,696 - -0.8 539,696 - -0.2 539,696 - +1.0 539,696 - +0.6 539,696 - -1.5 539,696 - +0.8 539,696 - -0.9 539,696 - -0.2 539,696 - -2.1 539,696 - -0.1 539,696 - +0.3 539,696 - +0.2 539,696 - +1.4 539,696 - -1.0 539,696 - -1.7 539,697 + +0.8 539,709 + +1.2 539,714 + -1.7 539,766 + -0.1 539,904 - CCNA_00526 0.52 +2.8 540,045 - +0.7 540,066 - -0.6 540,082 + -1.6 540,090 - +1.2 540,091 + +1.1 540,091 + +1.3 540,091 + +2.3 540,116 + +0.6 540,116 + +1.1 540,116 + +1.6 540,116 + +0.7 540,124 - +1.8 540,124 - -0.6 540,124 - +0.1 540,143 + +1.1 540,179 + +1.6 540,186 + +1.2 540,203 + +1.2 540,203 + +2.1 540,203 + +1.5 540,245 + CCNA_00527 0.17 +1.2 540,253 - CCNA_00527 0.19 -0.1 540,253 - CCNA_00527 0.19 -0.9 540,263 - CCNA_00527 0.21 -1.3 540,268 + CCNA_00527 0.22 +1.5 540,335 - CCNA_00527 0.35 +1.2 540,343 + CCNA_00527 0.37 +1.7 540,346 + CCNA_00527 0.37 +1.6 540,349 - CCNA_00527 0.38 +1.5 540,349 - CCNA_00527 0.38 +0.9 540,363 + CCNA_00527 0.41 +1.3 540,398 + CCNA_00527 0.48 +1.5 540,406 + CCNA_00527 0.49 +2.0 540,406 - CCNA_00527 0.49 -0.6 540,450 + CCNA_00527 0.58 +0.3 540,467 + CCNA_00527 0.61 +1.6 540,486 + CCNA_00527 0.65 +2.0 540,487 + CCNA_00527 0.65 +2.3 540,488 + CCNA_00527 0.65 +1.9 540,488 + CCNA_00527 0.65 +1.7 540,498 + CCNA_00527 0.67 +1.6 540,498 + CCNA_00527 0.67 +1.6 540,498 + CCNA_00527 0.67 +2.3 540,498 + CCNA_00527 0.67 +1.5 540,498 + CCNA_00527 0.67 +1.9 540,511 + CCNA_00527 0.70 +2.0 540,519 - CCNA_00527 0.71 +0.1 540,522 + CCNA_00527 0.72 +1.7 540,530 - CCNA_00527 0.74 +0.9 540,588 - CCNA_00527 0.85 +0.9 540,609 - CCNA_00527 0.89 +0.1 540,657 + +1.6 540,657 + +0.3 540,657 + +0.7 540,657 + +1.0 540,657 + -0.1 540,657 + +1.0 540,657 + +1.3 540,657 + +1.1 540,657 + -1.1 540,657 + -0.2 540,657 + +1.2 540,657 + +0.6 540,657 + +1.1 540,657 + +1.3 540,657 + +1.5 540,657 + +1.0 540,657 + +0.8 540,657 + +0.7 540,664 + +1.2 540,664 + +1.3 540,664 + +0.6 540,664 + +1.0 540,665 - -0.4 540,665 - +1.5 540,665 - +1.4 540,665 - +2.4 540,665 - +1.8 540,665 - +1.4 540,665 - -1.1 540,672 - -0.5 540,718 + +0.8 540,718 + +1.0 540,751 + +1.2 540,751 + +1.3 540,751 + +1.2 540,751 + +1.6 540,751 + +0.9 540,751 + +1.4 540,754 - -0.4 540,759 - -0.7 540,759 - -1.4 540,759 - +0.8 540,759 - -2.2 540,776 + +0.4 540,820 + +1.1 540,827 + +0.6 540,827 + +2.3 540,879 - -0.3 540,880 + +0.3 540,880 + -0.1 540,900 - +0.1 540,967 + CCNA_00528 0.16 -1.3 540,992 - +1.8 540,992 - CCNA_00528 0.19 -1.3 541,004 - CCNA_00528 0.20 -0.8 541,047 + CCNA_00528 0.26 +0.8 541,047 + CCNA_00528 0.26 +0.8 541,083 - CCNA_00528 0.31 +1.4 541,083 - CCNA_00528 0.31 -1.4 541,115 + CCNA_00528 0.35 +0.8 541,123 - CCNA_00528 0.36 +2.6 541,123 - CCNA_00528 0.36 -1.7 541,154 - +1.8 541,214 - CCNA_00528 0.47 -1.7 541,225 - CCNA_00528 0.49 -1.2 541,240 + CCNA_00528 0.51 +1.0 541,253 + CCNA_00528 0.52 +0.7 541,298 + CCNA_00528 0.58 -0.2 541,303 + CCNA_00528 0.59 -0.3 541,303 - CCNA_00528 0.59 +0.9 541,327 + CCNA_00528 0.62 +0.8 541,347 - CCNA_00528 0.64 -1.9 541,351 + CCNA_00528 0.65 +0.3 541,351 + CCNA_00528 0.65 -0.4 541,379 - CCNA_00528 0.68 -0.5 541,390 + CCNA_00528 0.70 -0.1 541,390 + CCNA_00528 0.70 -0.1 541,390 + CCNA_00528 0.70 +0.4 541,390 + CCNA_00528 0.70 +0.8 541,390 + CCNA_00528 0.70 +0.8 541,397 + CCNA_00528 0.71 +1.7 541,398 - CCNA_00528 0.71 -1.2 541,398 - CCNA_00528 0.71 -1.2 541,398 - CCNA_00528 0.71 -1.6 541,403 + CCNA_00528 0.71 +0.1 541,403 + CCNA_00528 0.71 +0.7 541,405 - CCNA_00528 0.72 +0.7 541,405 - CCNA_00528 0.72 +0.3 541,405 - +2.8 541,411 - CCNA_00528 0.72 +1.8 541,416 - CCNA_00528 0.73 -0.3 541,462 + CCNA_00528 0.79 +0.4 541,462 + CCNA_00528 0.79 +0.8 541,480 - CCNA_00528 0.81 -0.8 541,480 - CCNA_00528 0.81 +0.3 541,481 + CCNA_00528 0.81 +1.2 541,489 - CCNA_00528 0.82 +0.9 541,508 - CCNA_00528 0.85 +1.1 541,540 + CCNA_00528 0.89 +0.2 541,540 + CCNA_00528 0.89 +0.8 541,540 + CCNA_00528 0.89 +1.5 541,540 + CCNA_00528 0.89 +0.1 541,548 - +0.8 541,557 - -0.3 541,578 - +1.4 541,582 + +0.0 541,582 + +0.9 541,583 - -0.9 541,594 + -0.0 541,652 + +0.4 541,652 + -1.3 541,652 + -0.1 541,652 + +0.7 541,657 + -0.2 541,657 + +0.2 541,657 + -1.4 541,657 + +0.6
Or see this region's nucleotide sequence