Experiment: PYE with Spectinomycin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00317 and CCNA_00318 are separated by 207 nucleotides CCNA_00318 and CCNA_00319 are separated by 85 nucleotides CCNA_00319 and CCNA_00320 are separated by 182 nucleotides
CCNA_00317: CCNA_00317 - GTP-binding protein CgtA, at 331,907 to 332,971
_00317
CCNA_00318: CCNA_00318 - hypothetical protein, at 333,179 to 334,138
_00318
CCNA_00319: CCNA_00319 - acetyltransferase, GNAT family, at 334,224 to 334,757
_00319
CCNA_00320: CCNA_00320 - LSU ribosomal protein L27P, at 334,940 to 335,209
_00320
Position (kb) 333
334
335 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 333.016 kb on - strand at 333.036 kb on - strand at 333.037 kb on - strand at 333.056 kb on - strand at 333.066 kb on - strand at 333.081 kb on + strand at 333.232 kb on + strand at 333.256 kb on + strand at 333.264 kb on - strand at 333.268 kb on + strand at 333.364 kb on - strand, within CCNA_00318 at 333.377 kb on - strand, within CCNA_00318 at 333.381 kb on + strand, within CCNA_00318 at 333.396 kb on + strand, within CCNA_00318 at 333.416 kb on + strand, within CCNA_00318 at 333.419 kb on - strand, within CCNA_00318 at 333.419 kb on - strand, within CCNA_00318 at 333.419 kb on - strand, within CCNA_00318 at 333.419 kb on - strand, within CCNA_00318 at 333.419 kb on - strand, within CCNA_00318 at 333.424 kb on - strand, within CCNA_00318 at 333.444 kb on + strand, within CCNA_00318 at 333.456 kb on + strand, within CCNA_00318 at 333.464 kb on - strand, within CCNA_00318 at 333.481 kb on + strand, within CCNA_00318 at 333.515 kb on + strand, within CCNA_00318 at 333.523 kb on - strand, within CCNA_00318 at 333.652 kb on + strand, within CCNA_00318 at 333.652 kb on + strand, within CCNA_00318 at 333.652 kb on + strand, within CCNA_00318 at 333.652 kb on + strand, within CCNA_00318 at 333.652 kb on + strand, within CCNA_00318 at 333.652 kb on + strand, within CCNA_00318 at 333.652 kb on + strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.660 kb on - strand, within CCNA_00318 at 333.689 kb on + strand, within CCNA_00318 at 333.689 kb on - strand, within CCNA_00318 at 333.691 kb on + strand, within CCNA_00318 at 333.691 kb on + strand, within CCNA_00318 at 333.691 kb on + strand, within CCNA_00318 at 333.699 kb on - strand, within CCNA_00318 at 333.699 kb on - strand, within CCNA_00318 at 333.755 kb on - strand, within CCNA_00318 at 333.775 kb on - strand, within CCNA_00318 at 333.841 kb on - strand, within CCNA_00318 at 333.858 kb on - strand, within CCNA_00318 at 333.859 kb on - strand, within CCNA_00318 at 333.878 kb on - strand, within CCNA_00318 at 333.924 kb on - strand, within CCNA_00318 at 333.967 kb on + strand, within CCNA_00318 at 333.967 kb on - strand, within CCNA_00318 at 333.978 kb on + strand, within CCNA_00318 at 334.016 kb on - strand, within CCNA_00318 at 334.029 kb on + strand, within CCNA_00318 at 334.056 kb on + strand at 334.056 kb on + strand at 334.056 kb on + strand at 334.056 kb on + strand at 334.077 kb on - strand at 334.100 kb on + strand at 334.122 kb on - strand at 334.131 kb on - strand at 334.133 kb on + strand at 334.133 kb on + strand at 334.141 kb on - strand at 334.159 kb on - strand at 334.201 kb on + strand at 334.209 kb on - strand at 334.221 kb on - strand at 334.295 kb on + strand, within CCNA_00319 at 334.303 kb on - strand, within CCNA_00319 at 334.305 kb on - strand, within CCNA_00319 at 334.305 kb on - strand, within CCNA_00319 at 334.309 kb on + strand, within CCNA_00319 at 334.310 kb on + strand, within CCNA_00319 at 334.340 kb on - strand, within CCNA_00319 at 334.340 kb on - strand, within CCNA_00319 at 334.376 kb on + strand, within CCNA_00319 at 334.386 kb on + strand, within CCNA_00319 at 334.401 kb on + strand, within CCNA_00319 at 334.421 kb on + strand, within CCNA_00319 at 334.434 kb on + strand, within CCNA_00319 at 334.434 kb on + strand, within CCNA_00319 at 334.454 kb on - strand, within CCNA_00319 at 334.523 kb on - strand, within CCNA_00319 at 334.559 kb on - strand, within CCNA_00319 at 334.561 kb on + strand, within CCNA_00319 at 334.566 kb on + strand, within CCNA_00319 at 334.585 kb on + strand, within CCNA_00319 at 334.595 kb on + strand, within CCNA_00319 at 334.631 kb on - strand, within CCNA_00319 at 334.692 kb on - strand, within CCNA_00319 at 334.698 kb on - strand, within CCNA_00319 at 334.717 kb on - strand at 334.755 kb on + strand at 334.770 kb on + strand at 334.771 kb on - strand at 334.821 kb on - strand at 334.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml remove 333,016 - -1.7 333,036 - -1.6 333,037 - -0.1 333,056 - +0.2 333,066 - +0.7 333,081 + -0.4 333,232 + +0.5 333,256 + +1.2 333,264 - -3.0 333,268 + -0.5 333,364 - CCNA_00318 0.19 -0.2 333,377 - CCNA_00318 0.21 +0.7 333,381 + CCNA_00318 0.21 -1.3 333,396 + CCNA_00318 0.23 -0.5 333,416 + CCNA_00318 0.25 -0.1 333,419 - CCNA_00318 0.25 +0.4 333,419 - CCNA_00318 0.25 +2.0 333,419 - CCNA_00318 0.25 -2.5 333,419 - CCNA_00318 0.25 +0.2 333,419 - CCNA_00318 0.25 -1.2 333,424 - CCNA_00318 0.26 +0.2 333,444 + CCNA_00318 0.28 +0.4 333,456 + CCNA_00318 0.29 +0.8 333,464 - CCNA_00318 0.30 -0.4 333,481 + CCNA_00318 0.31 -1.2 333,515 + CCNA_00318 0.35 -1.4 333,523 - CCNA_00318 0.36 -0.8 333,652 + CCNA_00318 0.49 -1.3 333,652 + CCNA_00318 0.49 +0.6 333,652 + CCNA_00318 0.49 -1.6 333,652 + CCNA_00318 0.49 -0.3 333,652 + CCNA_00318 0.49 -1.4 333,652 + CCNA_00318 0.49 -0.3 333,652 + CCNA_00318 0.49 -0.9 333,660 - CCNA_00318 0.50 -1.9 333,660 - CCNA_00318 0.50 -0.4 333,660 - CCNA_00318 0.50 -0.1 333,660 - CCNA_00318 0.50 -1.3 333,660 - CCNA_00318 0.50 -0.6 333,660 - CCNA_00318 0.50 -1.6 333,660 - CCNA_00318 0.50 +0.2 333,660 - CCNA_00318 0.50 +0.6 333,660 - CCNA_00318 0.50 -1.8 333,660 - CCNA_00318 0.50 -0.2 333,660 - CCNA_00318 0.50 +0.0 333,660 - CCNA_00318 0.50 -0.1 333,660 - CCNA_00318 0.50 +1.2 333,660 - CCNA_00318 0.50 -0.1 333,660 - CCNA_00318 0.50 -0.8 333,689 + CCNA_00318 0.53 -2.5 333,689 - CCNA_00318 0.53 +1.1 333,691 + CCNA_00318 0.53 -2.4 333,691 + CCNA_00318 0.53 -1.2 333,691 + CCNA_00318 0.53 -2.1 333,699 - CCNA_00318 0.54 +0.2 333,699 - CCNA_00318 0.54 -1.6 333,755 - CCNA_00318 0.60 -0.1 333,775 - CCNA_00318 0.62 +1.7 333,841 - CCNA_00318 0.69 -1.8 333,858 - CCNA_00318 0.71 -0.9 333,859 - CCNA_00318 0.71 -1.2 333,878 - CCNA_00318 0.73 -0.8 333,924 - CCNA_00318 0.78 -0.9 333,967 + CCNA_00318 0.82 -1.8 333,967 - CCNA_00318 0.82 +0.3 333,978 + CCNA_00318 0.83 +0.1 334,016 - CCNA_00318 0.87 +1.1 334,029 + CCNA_00318 0.89 +0.7 334,056 + +0.0 334,056 + +0.5 334,056 + +0.7 334,056 + +0.1 334,077 - -0.0 334,100 + +0.1 334,122 - +1.4 334,131 - +0.3 334,133 + -2.1 334,133 + +1.9 334,141 - +1.5 334,159 - +0.1 334,201 + -0.6 334,209 - +0.8 334,221 - +0.8 334,295 + CCNA_00319 0.13 -2.0 334,303 - CCNA_00319 0.15 -1.1 334,305 - CCNA_00319 0.15 -0.6 334,305 - CCNA_00319 0.15 -0.2 334,309 + CCNA_00319 0.16 -0.8 334,310 + CCNA_00319 0.16 -0.8 334,340 - CCNA_00319 0.22 -0.4 334,340 - CCNA_00319 0.22 +0.3 334,376 + CCNA_00319 0.28 +0.5 334,386 + CCNA_00319 0.30 +0.1 334,401 + CCNA_00319 0.33 -1.0 334,421 + CCNA_00319 0.37 +1.3 334,434 + CCNA_00319 0.39 -0.7 334,434 + CCNA_00319 0.39 +1.3 334,454 - CCNA_00319 0.43 +0.2 334,523 - CCNA_00319 0.56 +0.9 334,559 - CCNA_00319 0.63 +0.3 334,561 + CCNA_00319 0.63 +1.2 334,566 + CCNA_00319 0.64 -0.8 334,585 + CCNA_00319 0.68 -0.8 334,595 + CCNA_00319 0.69 -1.3 334,631 - CCNA_00319 0.76 +0.0 334,692 - CCNA_00319 0.88 +0.1 334,698 - CCNA_00319 0.89 -0.6 334,717 - +1.9 334,755 + -1.8 334,770 + -1.3 334,771 - +0.6 334,821 - -0.4 334,903 - +1.1
Or see this region's nucleotide sequence