Experiment: PYE with Spectinomycin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00292 and CCNA_00293 overlap by 8 nucleotides CCNA_00293 and CCNA_00294 are separated by 59 nucleotides CCNA_00294 and CCNA_00295 are separated by 25 nucleotides
CCNA_00292: CCNA_00292 - phosphate transport system permease protein pstC, at 306,029 to 307,462
_00292
CCNA_00293: CCNA_00293 - phosphate transport system permease protein pstA, at 307,455 to 308,750
_00293
CCNA_00294: CCNA_00294 - phosphate transport ATP-binding protein pstB, at 308,810 to 309,634
_00294
CCNA_00295: CCNA_00295 - phosphate transport system protein phoU, at 309,660 to 310,352
_00295
Position (kb) 307
308
309 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 306.471 kb on + strand, within CCNA_00292 at 306.476 kb on + strand, within CCNA_00292 at 306.476 kb on + strand, within CCNA_00292 at 306.998 kb on + strand, within CCNA_00292 at 307.414 kb on - strand at 307.441 kb on - strand at 307.451 kb on - strand at 307.547 kb on + strand at 307.735 kb on + strand at 307.871 kb on + strand, within CCNA_00293 at 307.984 kb on + strand at 308.038 kb on + strand, within CCNA_00293 at 308.704 kb on - strand at 308.750 kb on + strand at 308.786 kb on - strand at 308.802 kb on - strand at 308.806 kb on + strand at 308.806 kb on + strand at 308.806 kb on + strand at 308.806 kb on + strand at 308.806 kb on + strand at 308.806 kb on + strand at 308.806 kb on + strand at 308.806 kb on + strand at 308.806 kb on + strand at 308.806 kb on + strand at 308.806 kb on + strand at 308.814 kb on - strand at 308.814 kb on - strand at 308.814 kb on - strand at 308.814 kb on - strand at 308.814 kb on - strand at 308.814 kb on - strand at 308.814 kb on - strand at 308.814 kb on - strand at 308.929 kb on + strand, within CCNA_00294 at 309.555 kb on + strand at 309.633 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml remove 306,471 + CCNA_00292 0.31 +2.1 306,476 + CCNA_00292 0.31 +1.1 306,476 + CCNA_00292 0.31 +2.7 306,998 + CCNA_00292 0.68 +0.3 307,414 - +2.0 307,441 - +1.3 307,451 - +3.5 307,547 + +1.4 307,735 + +0.9 307,871 + CCNA_00293 0.32 +2.8 307,984 + +1.9 308,038 + CCNA_00293 0.45 +2.2 308,704 - +1.6 308,750 + -0.3 308,786 - +0.6 308,802 - -0.6 308,806 + +0.7 308,806 + +1.3 308,806 + +0.4 308,806 + +0.2 308,806 + +0.5 308,806 + +1.2 308,806 + +0.3 308,806 + +0.6 308,806 + +1.2 308,806 + +0.0 308,806 + +0.6 308,814 - -2.4 308,814 - -1.3 308,814 - -1.1 308,814 - -0.3 308,814 - -2.1 308,814 - -2.5 308,814 - +0.6 308,814 - -0.0 308,929 + CCNA_00294 0.14 +0.5 309,555 + +2.1 309,633 + +0.7
Or see this region's nucleotide sequence