Experiment: Recovery broth with Moxifloxacin 0.004 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14875 and BBR_RS14880 are separated by 373 nucleotides BBR_RS14880 and BBR_RS14885 are separated by 39 nucleotides
BBR_RS14875: BBR_RS14875 - uracil-xanthine permease, at 1,110,074 to 1,111,363
_RS14875
BBR_RS14880: BBR_RS14880 - ATP-dependent helicase, at 1,111,737 to 1,113,764
_RS14880
BBR_RS14885: BBR_RS14885 - cyclomaltodextrinase, at 1,113,804 to 1,115,099
_RS14885
Position (kb)
1111
1112
1113
1114 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1110.768 kb on + strand, within BBR_RS14875 at 1110.775 kb on + strand, within BBR_RS14875 at 1110.778 kb on + strand, within BBR_RS14875 at 1110.829 kb on + strand, within BBR_RS14875 at 1110.829 kb on + strand, within BBR_RS14875 at 1110.842 kb on + strand, within BBR_RS14875 at 1110.892 kb on - strand, within BBR_RS14875 at 1111.003 kb on + strand, within BBR_RS14875 at 1111.040 kb on - strand, within BBR_RS14875 at 1111.073 kb on + strand, within BBR_RS14875 at 1111.106 kb on + strand, within BBR_RS14875 at 1111.108 kb on + strand, within BBR_RS14875 at 1111.116 kb on - strand, within BBR_RS14875 at 1111.182 kb on + strand, within BBR_RS14875 at 1111.182 kb on + strand, within BBR_RS14875 at 1111.215 kb on + strand, within BBR_RS14875 at 1111.237 kb on - strand at 1111.347 kb on + strand at 1111.391 kb on - strand at 1111.421 kb on - strand at 1111.444 kb on + strand at 1111.444 kb on + strand at 1111.504 kb on - strand at 1111.510 kb on - strand at 1111.573 kb on + strand at 1111.802 kb on + strand at 1111.889 kb on - strand at 1111.973 kb on - strand, within BBR_RS14880 at 1112.074 kb on + strand, within BBR_RS14880 at 1112.074 kb on + strand, within BBR_RS14880 at 1112.127 kb on - strand, within BBR_RS14880 at 1112.175 kb on + strand, within BBR_RS14880 at 1112.211 kb on + strand, within BBR_RS14880 at 1112.290 kb on + strand, within BBR_RS14880 at 1112.298 kb on - strand, within BBR_RS14880 at 1112.374 kb on + strand, within BBR_RS14880 at 1112.619 kb on - strand, within BBR_RS14880 at 1112.620 kb on + strand, within BBR_RS14880 at 1112.628 kb on + strand, within BBR_RS14880 at 1112.628 kb on - strand, within BBR_RS14880 at 1112.697 kb on + strand, within BBR_RS14880 at 1112.735 kb on + strand, within BBR_RS14880 at 1112.748 kb on + strand, within BBR_RS14880 at 1112.758 kb on + strand, within BBR_RS14880 at 1112.758 kb on + strand, within BBR_RS14880 at 1112.758 kb on + strand, within BBR_RS14880 at 1112.925 kb on - strand, within BBR_RS14880 at 1112.928 kb on - strand, within BBR_RS14880 at 1112.929 kb on + strand, within BBR_RS14880 at 1113.066 kb on - strand, within BBR_RS14880 at 1113.115 kb on - strand, within BBR_RS14880 at 1113.305 kb on + strand, within BBR_RS14880 at 1113.470 kb on + strand, within BBR_RS14880 at 1113.498 kb on - strand, within BBR_RS14880 at 1113.499 kb on + strand, within BBR_RS14880 at 1113.509 kb on + strand, within BBR_RS14880 at 1113.581 kb on - strand at 1113.777 kb on + strand at 1113.782 kb on - strand at 1113.790 kb on + strand at 1113.818 kb on + strand at 1113.826 kb on - strand at 1113.855 kb on + strand at 1113.857 kb on - strand at 1113.860 kb on + strand at 1113.880 kb on + strand at 1113.998 kb on + strand, within BBR_RS14885 at 1114.336 kb on + strand, within BBR_RS14885 at 1114.336 kb on + strand, within BBR_RS14885 at 1114.339 kb on - strand, within BBR_RS14885 at 1114.444 kb on + strand, within BBR_RS14885 at 1114.444 kb on + strand, within BBR_RS14885 at 1114.452 kb on - strand, within BBR_RS14885 at 1114.452 kb on - strand, within BBR_RS14885 at 1114.527 kb on - strand, within BBR_RS14885 at 1114.554 kb on - strand, within BBR_RS14885 at 1114.696 kb on - strand, within BBR_RS14885 at 1114.743 kb on + strand, within BBR_RS14885
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Moxifloxacin 0.004 mM remove 1,110,768 + BBR_RS14875 0.54 -1.0 1,110,775 + BBR_RS14875 0.54 -0.5 1,110,778 + BBR_RS14875 0.55 +0.4 1,110,829 + BBR_RS14875 0.59 -1.4 1,110,829 + BBR_RS14875 0.59 +0.6 1,110,842 + BBR_RS14875 0.60 +0.1 1,110,892 - BBR_RS14875 0.63 +0.6 1,111,003 + BBR_RS14875 0.72 -0.3 1,111,040 - BBR_RS14875 0.75 -0.4 1,111,073 + BBR_RS14875 0.77 -0.0 1,111,106 + BBR_RS14875 0.80 -0.7 1,111,108 + BBR_RS14875 0.80 -0.6 1,111,116 - BBR_RS14875 0.81 +1.3 1,111,182 + BBR_RS14875 0.86 +0.8 1,111,182 + BBR_RS14875 0.86 -1.4 1,111,215 + BBR_RS14875 0.88 +0.4 1,111,237 - -0.4 1,111,347 + -0.7 1,111,391 - -0.5 1,111,421 - +1.1 1,111,444 + +1.7 1,111,444 + -1.5 1,111,504 - +0.8 1,111,510 - -1.1 1,111,573 + +0.4 1,111,802 + -0.1 1,111,889 - -0.1 1,111,973 - BBR_RS14880 0.12 +1.6 1,112,074 + BBR_RS14880 0.17 +0.4 1,112,074 + BBR_RS14880 0.17 +2.7 1,112,127 - BBR_RS14880 0.19 +0.6 1,112,175 + BBR_RS14880 0.22 +0.0 1,112,211 + BBR_RS14880 0.23 +0.8 1,112,290 + BBR_RS14880 0.27 +0.5 1,112,298 - BBR_RS14880 0.28 +0.4 1,112,374 + BBR_RS14880 0.31 +1.0 1,112,619 - BBR_RS14880 0.43 +1.0 1,112,620 + BBR_RS14880 0.44 +0.4 1,112,628 + BBR_RS14880 0.44 +0.1 1,112,628 - BBR_RS14880 0.44 +1.1 1,112,697 + BBR_RS14880 0.47 -1.0 1,112,735 + BBR_RS14880 0.49 -0.6 1,112,748 + BBR_RS14880 0.50 +0.1 1,112,758 + BBR_RS14880 0.50 +0.5 1,112,758 + BBR_RS14880 0.50 +1.2 1,112,758 + BBR_RS14880 0.50 +0.0 1,112,925 - BBR_RS14880 0.59 +1.7 1,112,928 - BBR_RS14880 0.59 +0.8 1,112,929 + BBR_RS14880 0.59 +1.7 1,113,066 - BBR_RS14880 0.66 +1.0 1,113,115 - BBR_RS14880 0.68 -2.2 1,113,305 + BBR_RS14880 0.77 +0.8 1,113,470 + BBR_RS14880 0.85 +0.7 1,113,498 - BBR_RS14880 0.87 -1.3 1,113,499 + BBR_RS14880 0.87 +0.7 1,113,509 + BBR_RS14880 0.87 -0.2 1,113,581 - +0.7 1,113,777 + +0.2 1,113,782 - +1.8 1,113,790 + -0.5 1,113,818 + -0.4 1,113,826 - -0.3 1,113,855 + -1.9 1,113,857 - -0.6 1,113,860 + -0.2 1,113,880 + -0.1 1,113,998 + BBR_RS14885 0.15 -0.8 1,114,336 + BBR_RS14885 0.41 -0.3 1,114,336 + BBR_RS14885 0.41 -1.5 1,114,339 - BBR_RS14885 0.41 +0.4 1,114,444 + BBR_RS14885 0.49 -1.5 1,114,444 + BBR_RS14885 0.49 -1.0 1,114,452 - BBR_RS14885 0.50 -1.2 1,114,452 - BBR_RS14885 0.50 -1.1 1,114,527 - BBR_RS14885 0.56 -1.3 1,114,554 - BBR_RS14885 0.58 -1.9 1,114,696 - BBR_RS14885 0.69 -2.4 1,114,743 + BBR_RS14885 0.72 -1.4
Or see this region's nucleotide sequence