Experiment: benzoic 0.58 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_611 and Pf1N1B4_612 are separated by 133 nucleotides Pf1N1B4_612 and Pf1N1B4_613 overlap by 4 nucleotides
Pf1N1B4_611 - CoA transferase, CAIB/BAIF family, at 705,554 to 706,885
_611
Pf1N1B4_612 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 707,019 to 707,882
_612
Pf1N1B4_613 - Arginine/ornithine antiporter ArcD, at 707,879 to 709,306
_613
Position (kb)
707
708 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 706.516 kb on - strand, within Pf1N1B4_611 at 706.613 kb on + strand, within Pf1N1B4_611 at 706.997 kb on - strand at 707.063 kb on - strand at 707.209 kb on + strand, within Pf1N1B4_612 at 707.210 kb on - strand, within Pf1N1B4_612 at 707.210 kb on - strand, within Pf1N1B4_612 at 707.210 kb on - strand, within Pf1N1B4_612 at 707.210 kb on - strand, within Pf1N1B4_612 at 707.210 kb on - strand, within Pf1N1B4_612 at 707.397 kb on + strand, within Pf1N1B4_612 at 707.397 kb on + strand, within Pf1N1B4_612 at 707.398 kb on - strand at 707.398 kb on - strand, within Pf1N1B4_612 at 707.398 kb on - strand, within Pf1N1B4_612 at 707.398 kb on - strand, within Pf1N1B4_612 at 707.398 kb on - strand, within Pf1N1B4_612 at 707.398 kb on - strand, within Pf1N1B4_612 at 707.777 kb on - strand, within Pf1N1B4_612 at 707.851 kb on + strand at 707.852 kb on - strand at 707.901 kb on + strand at 707.902 kb on - strand at 707.902 kb on - strand at 707.902 kb on - strand at 707.902 kb on - strand at 707.902 kb on - strand at 707.902 kb on - strand at 707.969 kb on - strand at 708.027 kb on + strand, within Pf1N1B4_613 at 708.028 kb on - strand, within Pf1N1B4_613 at 708.070 kb on - strand, within Pf1N1B4_613 at 708.070 kb on - strand, within Pf1N1B4_613 at 708.070 kb on - strand, within Pf1N1B4_613 at 708.070 kb on - strand, within Pf1N1B4_613 at 708.148 kb on - strand, within Pf1N1B4_613 at 708.148 kb on - strand, within Pf1N1B4_613 at 708.148 kb on - strand, within Pf1N1B4_613 at 708.148 kb on - strand, within Pf1N1B4_613 at 708.148 kb on - strand, within Pf1N1B4_613 at 708.181 kb on - strand, within Pf1N1B4_613 at 708.199 kb on - strand, within Pf1N1B4_613 at 708.199 kb on - strand, within Pf1N1B4_613 at 708.199 kb on - strand, within Pf1N1B4_613 at 708.199 kb on - strand, within Pf1N1B4_613 at 708.228 kb on + strand, within Pf1N1B4_613 at 708.228 kb on + strand, within Pf1N1B4_613 at 708.229 kb on - strand, within Pf1N1B4_613 at 708.229 kb on - strand at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.244 kb on - strand, within Pf1N1B4_613 at 708.244 kb on - strand, within Pf1N1B4_613 at 708.244 kb on - strand, within Pf1N1B4_613 at 708.244 kb on - strand, within Pf1N1B4_613 at 708.437 kb on - strand, within Pf1N1B4_613 at 708.481 kb on + strand, within Pf1N1B4_613 at 708.481 kb on + strand, within Pf1N1B4_613 at 708.481 kb on + strand, within Pf1N1B4_613 at 708.482 kb on - strand, within Pf1N1B4_613 at 708.592 kb on + strand, within Pf1N1B4_613 at 708.649 kb on - strand, within Pf1N1B4_613
Per-strain Table
Position Strand Gene LocusTag Fraction benzoic 0.58 mg/ml remove 706,516 - Pf1N1B4_611 0.72 +0.2 706,613 + Pf1N1B4_611 0.80 -1.4 706,997 - +1.8 707,063 - +0.3 707,209 + Pf1N1B4_612 0.22 -0.5 707,210 - Pf1N1B4_612 0.22 -0.9 707,210 - Pf1N1B4_612 0.22 -0.1 707,210 - Pf1N1B4_612 0.22 -0.7 707,210 - Pf1N1B4_612 0.22 -0.7 707,210 - Pf1N1B4_612 0.22 +2.9 707,397 + Pf1N1B4_612 0.44 -1.4 707,397 + Pf1N1B4_612 0.44 -2.1 707,398 - -1.1 707,398 - Pf1N1B4_612 0.44 +0.7 707,398 - Pf1N1B4_612 0.44 -1.1 707,398 - Pf1N1B4_612 0.44 -1.4 707,398 - Pf1N1B4_612 0.44 -1.9 707,398 - Pf1N1B4_612 0.44 -0.5 707,777 - Pf1N1B4_612 0.88 -1.7 707,851 + +1.1 707,852 - -0.3 707,901 + -1.1 707,902 - +0.9 707,902 - +0.8 707,902 - -0.9 707,902 - -0.1 707,902 - -0.3 707,902 - -0.2 707,969 - -0.4 708,027 + Pf1N1B4_613 0.10 +0.9 708,028 - Pf1N1B4_613 0.10 +0.1 708,070 - Pf1N1B4_613 0.13 -0.1 708,070 - Pf1N1B4_613 0.13 -0.1 708,070 - Pf1N1B4_613 0.13 -1.4 708,070 - Pf1N1B4_613 0.13 +0.8 708,148 - Pf1N1B4_613 0.19 -1.4 708,148 - Pf1N1B4_613 0.19 +0.0 708,148 - Pf1N1B4_613 0.19 +0.3 708,148 - Pf1N1B4_613 0.19 -0.1 708,148 - Pf1N1B4_613 0.19 -1.7 708,181 - Pf1N1B4_613 0.21 -0.7 708,199 - Pf1N1B4_613 0.22 -0.5 708,199 - Pf1N1B4_613 0.22 +1.3 708,199 - Pf1N1B4_613 0.22 +0.9 708,199 - Pf1N1B4_613 0.22 -1.9 708,228 + Pf1N1B4_613 0.24 +1.2 708,228 + Pf1N1B4_613 0.24 -0.1 708,229 - Pf1N1B4_613 0.25 +0.2 708,229 - -0.1 708,243 + Pf1N1B4_613 0.25 -0.1 708,243 + Pf1N1B4_613 0.25 -1.3 708,243 + Pf1N1B4_613 0.25 +0.3 708,243 + Pf1N1B4_613 0.25 -0.5 708,243 + Pf1N1B4_613 0.25 +0.2 708,243 + Pf1N1B4_613 0.25 -0.2 708,243 + Pf1N1B4_613 0.25 +0.4 708,244 - Pf1N1B4_613 0.26 -1.4 708,244 - Pf1N1B4_613 0.26 -1.3 708,244 - Pf1N1B4_613 0.26 -0.1 708,244 - Pf1N1B4_613 0.26 +0.7 708,437 - Pf1N1B4_613 0.39 +0.5 708,481 + Pf1N1B4_613 0.42 +0.6 708,481 + Pf1N1B4_613 0.42 -1.7 708,481 + Pf1N1B4_613 0.42 -1.1 708,482 - Pf1N1B4_613 0.42 -0.1 708,592 + Pf1N1B4_613 0.50 +0.4 708,649 - Pf1N1B4_613 0.54 -1.7
Or see this region's nucleotide sequence