Experiment: benzoic 0.58 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4825 and Pf1N1B4_4826 are separated by 157 nucleotides Pf1N1B4_4826 and Pf1N1B4_4827 are separated by 164 nucleotides
Pf1N1B4_4825 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 5,329,241 to 5,330,329
_4825
Pf1N1B4_4826 - glutamine synthetase family protein, at 5,330,487 to 5,331,845
_4826
Pf1N1B4_4827 - Ethanolamine operon regulatory protein, at 5,332,010 to 5,332,936
_4827
Position (kb) 5330
5331
5332 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5329.686 kb on + strand, within Pf1N1B4_4825 at 5329.695 kb on + strand, within Pf1N1B4_4825 at 5329.696 kb on - strand, within Pf1N1B4_4825 at 5329.696 kb on - strand, within Pf1N1B4_4825 at 5329.732 kb on + strand, within Pf1N1B4_4825 at 5329.773 kb on + strand at 5330.172 kb on + strand, within Pf1N1B4_4825 at 5330.173 kb on - strand, within Pf1N1B4_4825 at 5330.229 kb on + strand at 5330.245 kb on - strand at 5330.277 kb on - strand at 5330.321 kb on + strand at 5330.342 kb on + strand at 5330.342 kb on + strand at 5330.343 kb on - strand at 5330.348 kb on + strand at 5330.348 kb on + strand at 5330.349 kb on - strand at 5330.957 kb on - strand, within Pf1N1B4_4826 at 5331.640 kb on + strand, within Pf1N1B4_4826 at 5331.643 kb on + strand, within Pf1N1B4_4826 at 5331.643 kb on + strand, within Pf1N1B4_4826 at 5331.643 kb on + strand at 5331.643 kb on + strand, within Pf1N1B4_4826 at 5331.644 kb on - strand, within Pf1N1B4_4826 at 5331.858 kb on - strand at 5331.958 kb on + strand at 5331.959 kb on - strand at 5331.959 kb on - strand at 5331.967 kb on + strand at 5332.170 kb on - strand at 5332.262 kb on + strand at 5332.523 kb on + strand at 5332.523 kb on + strand, within Pf1N1B4_4827 at 5332.524 kb on - strand, within Pf1N1B4_4827 at 5332.524 kb on - strand, within Pf1N1B4_4827 at 5332.709 kb on + strand, within Pf1N1B4_4827 at 5332.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction benzoic 0.58 mg/ml remove 5,329,686 + Pf1N1B4_4825 0.41 +0.6 5,329,695 + Pf1N1B4_4825 0.42 +0.9 5,329,696 - Pf1N1B4_4825 0.42 -2.1 5,329,696 - Pf1N1B4_4825 0.42 -1.7 5,329,732 + Pf1N1B4_4825 0.45 -1.1 5,329,773 + +0.4 5,330,172 + Pf1N1B4_4825 0.85 -0.6 5,330,173 - Pf1N1B4_4825 0.86 -0.6 5,330,229 + -1.1 5,330,245 - -1.1 5,330,277 - -0.9 5,330,321 + +0.1 5,330,342 + -0.3 5,330,342 + +2.0 5,330,343 - -1.1 5,330,348 + -0.1 5,330,348 + -0.9 5,330,349 - -1.6 5,330,957 - Pf1N1B4_4826 0.35 +1.0 5,331,640 + Pf1N1B4_4826 0.85 -1.1 5,331,643 + Pf1N1B4_4826 0.85 +2.9 5,331,643 + Pf1N1B4_4826 0.85 +0.4 5,331,643 + +0.4 5,331,643 + Pf1N1B4_4826 0.85 +0.8 5,331,644 - Pf1N1B4_4826 0.85 -0.8 5,331,858 - -0.1 5,331,958 + -0.0 5,331,959 - +1.4 5,331,959 - +1.0 5,331,967 + -0.8 5,332,170 - -0.9 5,332,262 + -0.1 5,332,523 + -0.1 5,332,523 + Pf1N1B4_4827 0.55 +0.9 5,332,524 - Pf1N1B4_4827 0.55 +0.7 5,332,524 - Pf1N1B4_4827 0.55 -1.0 5,332,709 + Pf1N1B4_4827 0.75 -0.8 5,332,710 - +0.9
Or see this region's nucleotide sequence