Experiment: benzoic 0.58 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4704 and Pf1N1B4_4705 are separated by 267 nucleotides Pf1N1B4_4705 and Pf1N1B4_4706 are separated by 2 nucleotides Pf1N1B4_4706 and Pf1N1B4_4707 are separated by 122 nucleotides Pf1N1B4_4707 and Pf1N1B4_4708 are separated by 165 nucleotides
Pf1N1B4_4704 - hypothetical protein, at 5,198,896 to 5,203,722
_4704
Pf1N1B4_4705 - Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1), at 5,203,990 to 5,204,832
_4705
Pf1N1B4_4706 - Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1), at 5,204,835 to 5,205,029
_4706
Pf1N1B4_4707 - Probable transmembrane protein, at 5,205,152 to 5,205,469
_4707
Pf1N1B4_4708 - FIG00958097: hypothetical protein, at 5,205,635 to 5,205,886
_4708
Position (kb)
5203
5204
5205 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5203.454 kb on + strand at 5203.455 kb on - strand at 5203.779 kb on - strand at 5203.806 kb on - strand at 5203.809 kb on + strand at 5203.813 kb on + strand at 5203.971 kb on - strand at 5204.012 kb on + strand at 5204.013 kb on - strand at 5204.016 kb on + strand at 5204.016 kb on + strand at 5204.364 kb on + strand, within Pf1N1B4_4705 at 5204.364 kb on + strand, within Pf1N1B4_4705 at 5204.583 kb on - strand, within Pf1N1B4_4705 at 5204.583 kb on - strand, within Pf1N1B4_4705 at 5204.583 kb on - strand, within Pf1N1B4_4705 at 5204.707 kb on - strand, within Pf1N1B4_4705 at 5204.738 kb on + strand, within Pf1N1B4_4705 at 5205.137 kb on + strand at 5205.137 kb on + strand at 5205.234 kb on + strand at 5205.475 kb on + strand at 5205.475 kb on + strand at 5205.476 kb on - strand at 5205.592 kb on + strand at 5205.592 kb on + strand at 5205.592 kb on + strand at 5205.592 kb on + strand at 5205.592 kb on + strand at 5205.593 kb on - strand at 5205.593 kb on - strand at 5205.593 kb on - strand at 5205.599 kb on + strand at 5205.600 kb on - strand at 5205.600 kb on - strand at 5205.611 kb on + strand at 5205.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction benzoic 0.58 mg/ml remove 5,203,454 + -1.0 5,203,455 - -0.8 5,203,779 - +0.5 5,203,806 - -0.1 5,203,809 + -0.9 5,203,813 + +1.5 5,203,971 - -0.1 5,204,012 + +0.5 5,204,013 - +0.5 5,204,016 + -0.1 5,204,016 + -2.0 5,204,364 + Pf1N1B4_4705 0.44 -1.1 5,204,364 + Pf1N1B4_4705 0.44 -0.1 5,204,583 - Pf1N1B4_4705 0.70 -0.9 5,204,583 - Pf1N1B4_4705 0.70 -1.1 5,204,583 - Pf1N1B4_4705 0.70 +0.2 5,204,707 - Pf1N1B4_4705 0.85 -0.8 5,204,738 + Pf1N1B4_4705 0.89 -0.9 5,205,137 + +0.5 5,205,137 + +0.5 5,205,234 + +0.5 5,205,475 + -0.3 5,205,475 + -0.1 5,205,476 - -1.0 5,205,592 + -0.7 5,205,592 + -0.3 5,205,592 + -1.1 5,205,592 + -0.4 5,205,592 + -0.5 5,205,593 - +0.7 5,205,593 - +2.5 5,205,593 - +0.2 5,205,599 + +0.2 5,205,600 - +0.1 5,205,600 - +0.3 5,205,611 + +1.5 5,205,617 - -0.5
Or see this region's nucleotide sequence