Experiment: benzoic 0.58 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2246 and Pf1N1B4_2247 are separated by 352 nucleotides Pf1N1B4_2247 and Pf1N1B4_2248 are separated by 102 nucleotides
Pf1N1B4_2246 - Alkyl hydroperoxide reductase subunit C-like protein, at 2,562,895 to 2,563,533
_2246
Pf1N1B4_2247 - FMN reductase (EC 1.5.1.29), at 2,563,886 to 2,564,479
_2247
Pf1N1B4_2248 - Alkanesulfonates-binding protein, at 2,564,582 to 2,565,547
_2248
Position (kb)
2563
2564
2565 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2562.981 kb on + strand, within Pf1N1B4_2246 at 2562.982 kb on - strand, within Pf1N1B4_2246 at 2563.216 kb on + strand, within Pf1N1B4_2246 at 2563.216 kb on + strand, within Pf1N1B4_2246 at 2563.216 kb on + strand, within Pf1N1B4_2246 at 2563.216 kb on + strand, within Pf1N1B4_2246 at 2563.216 kb on + strand, within Pf1N1B4_2246 at 2563.216 kb on + strand, within Pf1N1B4_2246 at 2563.217 kb on - strand, within Pf1N1B4_2246 at 2563.217 kb on - strand, within Pf1N1B4_2246 at 2563.256 kb on + strand, within Pf1N1B4_2246 at 2563.256 kb on + strand, within Pf1N1B4_2246 at 2563.256 kb on + strand, within Pf1N1B4_2246 at 2563.256 kb on + strand, within Pf1N1B4_2246 at 2563.256 kb on + strand, within Pf1N1B4_2246 at 2563.256 kb on + strand, within Pf1N1B4_2246 at 2563.256 kb on + strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.257 kb on - strand, within Pf1N1B4_2246 at 2563.263 kb on + strand, within Pf1N1B4_2246 at 2563.305 kb on + strand, within Pf1N1B4_2246 at 2563.310 kb on - strand, within Pf1N1B4_2246 at 2563.310 kb on - strand, within Pf1N1B4_2246 at 2563.310 kb on - strand, within Pf1N1B4_2246 at 2563.310 kb on - strand, within Pf1N1B4_2246 at 2563.381 kb on + strand, within Pf1N1B4_2246 at 2563.381 kb on + strand, within Pf1N1B4_2246 at 2563.381 kb on + strand, within Pf1N1B4_2246 at 2563.381 kb on + strand, within Pf1N1B4_2246 at 2563.381 kb on + strand, within Pf1N1B4_2246 at 2563.381 kb on + strand, within Pf1N1B4_2246 at 2563.381 kb on + strand, within Pf1N1B4_2246 at 2563.381 kb on + strand, within Pf1N1B4_2246 at 2563.382 kb on - strand, within Pf1N1B4_2246 at 2563.382 kb on - strand, within Pf1N1B4_2246 at 2563.382 kb on - strand, within Pf1N1B4_2246 at 2563.531 kb on + strand at 2563.531 kb on + strand at 2563.531 kb on + strand at 2563.532 kb on - strand at 2563.626 kb on + strand at 2563.626 kb on + strand at 2563.627 kb on - strand at 2563.696 kb on - strand at 2563.806 kb on + strand at 2563.818 kb on + strand at 2563.818 kb on + strand at 2563.819 kb on - strand at 2563.994 kb on + strand, within Pf1N1B4_2247 at 2563.994 kb on + strand, within Pf1N1B4_2247 at 2564.002 kb on - strand, within Pf1N1B4_2247 at 2564.051 kb on - strand, within Pf1N1B4_2247 at 2564.157 kb on + strand, within Pf1N1B4_2247 at 2564.158 kb on - strand, within Pf1N1B4_2247 at 2564.225 kb on - strand, within Pf1N1B4_2247 at 2564.225 kb on - strand, within Pf1N1B4_2247 at 2564.478 kb on - strand at 2564.478 kb on - strand at 2564.478 kb on - strand at 2564.478 kb on - strand at 2564.478 kb on - strand at 2564.511 kb on + strand at 2564.634 kb on + strand at 2564.650 kb on + strand at 2564.693 kb on + strand, within Pf1N1B4_2248 at 2564.694 kb on - strand, within Pf1N1B4_2248 at 2564.888 kb on + strand, within Pf1N1B4_2248 at 2564.888 kb on + strand, within Pf1N1B4_2248 at 2564.888 kb on + strand, within Pf1N1B4_2248 at 2564.888 kb on + strand, within Pf1N1B4_2248 at 2564.888 kb on + strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2564.889 kb on - strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.014 kb on + strand, within Pf1N1B4_2248 at 2565.015 kb on - strand, within Pf1N1B4_2248 at 2565.015 kb on - strand at 2565.053 kb on + strand, within Pf1N1B4_2248 at 2565.053 kb on + strand, within Pf1N1B4_2248 at 2565.053 kb on + strand, within Pf1N1B4_2248 at 2565.054 kb on - strand, within Pf1N1B4_2248 at 2565.054 kb on - strand, within Pf1N1B4_2248 at 2565.054 kb on - strand, within Pf1N1B4_2248 at 2565.146 kb on + strand, within Pf1N1B4_2248 at 2565.224 kb on + strand, within Pf1N1B4_2248 at 2565.225 kb on - strand, within Pf1N1B4_2248 at 2565.225 kb on - strand, within Pf1N1B4_2248 at 2565.225 kb on - strand, within Pf1N1B4_2248 at 2565.242 kb on + strand, within Pf1N1B4_2248 at 2565.242 kb on + strand, within Pf1N1B4_2248 at 2565.243 kb on - strand, within Pf1N1B4_2248 at 2565.243 kb on - strand, within Pf1N1B4_2248 at 2565.243 kb on - strand, within Pf1N1B4_2248 at 2565.418 kb on + strand, within Pf1N1B4_2248 at 2565.429 kb on + strand, within Pf1N1B4_2248 at 2565.429 kb on + strand, within Pf1N1B4_2248 at 2565.429 kb on + strand, within Pf1N1B4_2248 at 2565.430 kb on - strand, within Pf1N1B4_2248 at 2565.430 kb on - strand, within Pf1N1B4_2248 at 2565.464 kb on + strand at 2565.465 kb on - strand at 2565.465 kb on - strand at 2565.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction benzoic 0.58 mg/ml remove 2,562,981 + Pf1N1B4_2246 0.13 -2.0 2,562,982 - Pf1N1B4_2246 0.14 -0.3 2,563,216 + Pf1N1B4_2246 0.50 +0.3 2,563,216 + Pf1N1B4_2246 0.50 +1.6 2,563,216 + Pf1N1B4_2246 0.50 -1.7 2,563,216 + Pf1N1B4_2246 0.50 -1.7 2,563,216 + Pf1N1B4_2246 0.50 +0.1 2,563,216 + Pf1N1B4_2246 0.50 +0.8 2,563,217 - Pf1N1B4_2246 0.50 +1.1 2,563,217 - Pf1N1B4_2246 0.50 +1.0 2,563,256 + Pf1N1B4_2246 0.56 -0.3 2,563,256 + Pf1N1B4_2246 0.56 -1.0 2,563,256 + Pf1N1B4_2246 0.56 -0.6 2,563,256 + Pf1N1B4_2246 0.56 +0.5 2,563,256 + Pf1N1B4_2246 0.56 -0.4 2,563,256 + Pf1N1B4_2246 0.56 +0.0 2,563,256 + Pf1N1B4_2246 0.56 +0.0 2,563,257 - Pf1N1B4_2246 0.57 -2.0 2,563,257 - Pf1N1B4_2246 0.57 -1.0 2,563,257 - Pf1N1B4_2246 0.57 -2.4 2,563,257 - Pf1N1B4_2246 0.57 -0.3 2,563,257 - Pf1N1B4_2246 0.57 -2.0 2,563,257 - Pf1N1B4_2246 0.57 +0.1 2,563,257 - Pf1N1B4_2246 0.57 +0.6 2,563,257 - Pf1N1B4_2246 0.57 +1.3 2,563,257 - Pf1N1B4_2246 0.57 +1.7 2,563,257 - Pf1N1B4_2246 0.57 -0.1 2,563,257 - Pf1N1B4_2246 0.57 +0.9 2,563,257 - Pf1N1B4_2246 0.57 +0.1 2,563,263 + Pf1N1B4_2246 0.58 +1.1 2,563,305 + Pf1N1B4_2246 0.64 +0.1 2,563,310 - Pf1N1B4_2246 0.65 +0.7 2,563,310 - Pf1N1B4_2246 0.65 +0.6 2,563,310 - Pf1N1B4_2246 0.65 -0.5 2,563,310 - Pf1N1B4_2246 0.65 +1.3 2,563,381 + Pf1N1B4_2246 0.76 -1.5 2,563,381 + Pf1N1B4_2246 0.76 +0.0 2,563,381 + Pf1N1B4_2246 0.76 +1.9 2,563,381 + Pf1N1B4_2246 0.76 -2.6 2,563,381 + Pf1N1B4_2246 0.76 -1.3 2,563,381 + Pf1N1B4_2246 0.76 +0.2 2,563,381 + Pf1N1B4_2246 0.76 -0.3 2,563,381 + Pf1N1B4_2246 0.76 +0.0 2,563,382 - Pf1N1B4_2246 0.76 +0.7 2,563,382 - Pf1N1B4_2246 0.76 +1.6 2,563,382 - Pf1N1B4_2246 0.76 +0.3 2,563,531 + -1.0 2,563,531 + -0.4 2,563,531 + +0.7 2,563,532 - -1.5 2,563,626 + -0.1 2,563,626 + -1.3 2,563,627 - +1.5 2,563,696 - +1.3 2,563,806 + +0.1 2,563,818 + +1.0 2,563,818 + -1.3 2,563,819 - -1.7 2,563,994 + Pf1N1B4_2247 0.18 +0.0 2,563,994 + Pf1N1B4_2247 0.18 +0.3 2,564,002 - Pf1N1B4_2247 0.20 +0.1 2,564,051 - Pf1N1B4_2247 0.28 +1.3 2,564,157 + Pf1N1B4_2247 0.46 -1.3 2,564,158 - Pf1N1B4_2247 0.46 -2.1 2,564,225 - Pf1N1B4_2247 0.57 -0.3 2,564,225 - Pf1N1B4_2247 0.57 -1.0 2,564,478 - +1.6 2,564,478 - -1.5 2,564,478 - +0.3 2,564,478 - +0.7 2,564,478 - +0.2 2,564,511 + -0.2 2,564,634 + -0.8 2,564,650 + +0.7 2,564,693 + Pf1N1B4_2248 0.11 -0.4 2,564,694 - Pf1N1B4_2248 0.12 +0.3 2,564,888 + Pf1N1B4_2248 0.32 +0.6 2,564,888 + Pf1N1B4_2248 0.32 -2.5 2,564,888 + Pf1N1B4_2248 0.32 -0.3 2,564,888 + Pf1N1B4_2248 0.32 -0.7 2,564,888 + Pf1N1B4_2248 0.32 -2.3 2,564,889 - Pf1N1B4_2248 0.32 -0.5 2,564,889 - Pf1N1B4_2248 0.32 +0.5 2,564,889 - +0.7 2,564,889 - Pf1N1B4_2248 0.32 -1.8 2,564,889 - Pf1N1B4_2248 0.32 +1.3 2,564,889 - Pf1N1B4_2248 0.32 +0.3 2,564,889 - Pf1N1B4_2248 0.32 -1.3 2,564,889 - Pf1N1B4_2248 0.32 +0.3 2,564,889 - Pf1N1B4_2248 0.32 -0.5 2,564,889 - Pf1N1B4_2248 0.32 -1.3 2,564,889 - Pf1N1B4_2248 0.32 -1.3 2,565,014 + Pf1N1B4_2248 0.45 -1.1 2,565,014 + Pf1N1B4_2248 0.45 +0.7 2,565,014 + Pf1N1B4_2248 0.45 -0.7 2,565,014 + Pf1N1B4_2248 0.45 -0.9 2,565,014 + Pf1N1B4_2248 0.45 -1.0 2,565,014 + Pf1N1B4_2248 0.45 +0.4 2,565,015 - Pf1N1B4_2248 0.45 -2.0 2,565,015 - +1.3 2,565,053 + Pf1N1B4_2248 0.49 -0.2 2,565,053 + Pf1N1B4_2248 0.49 -1.5 2,565,053 + Pf1N1B4_2248 0.49 -0.5 2,565,054 - Pf1N1B4_2248 0.49 +1.3 2,565,054 - Pf1N1B4_2248 0.49 +0.1 2,565,054 - Pf1N1B4_2248 0.49 -0.7 2,565,146 + Pf1N1B4_2248 0.58 +1.3 2,565,224 + Pf1N1B4_2248 0.66 +0.9 2,565,225 - Pf1N1B4_2248 0.67 -0.8 2,565,225 - Pf1N1B4_2248 0.67 -0.1 2,565,225 - Pf1N1B4_2248 0.67 +0.9 2,565,242 + Pf1N1B4_2248 0.68 +1.9 2,565,242 + Pf1N1B4_2248 0.68 +1.6 2,565,243 - Pf1N1B4_2248 0.68 +0.7 2,565,243 - Pf1N1B4_2248 0.68 +0.7 2,565,243 - Pf1N1B4_2248 0.68 -1.3 2,565,418 + Pf1N1B4_2248 0.87 +0.2 2,565,429 + Pf1N1B4_2248 0.88 -0.7 2,565,429 + Pf1N1B4_2248 0.88 -0.5 2,565,429 + Pf1N1B4_2248 0.88 +1.2 2,565,430 - Pf1N1B4_2248 0.88 +2.0 2,565,430 - Pf1N1B4_2248 0.88 -0.4 2,565,464 + -0.7 2,565,465 - -0.3 2,565,465 - +0.8 2,565,465 - -1.8
Or see this region's nucleotide sequence