Experiment: benzoic 0.58 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_181 and Pf1N1B4_182 overlap by 4 nucleotides Pf1N1B4_182 and Pf1N1B4_183 overlap by 4 nucleotides
Pf1N1B4_181 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 224,292 to 225,233
_181
Pf1N1B4_182 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 225,230 to 225,985
_182
Pf1N1B4_183 - Major facilitator family transporter, at 225,982 to 227,283
_183
Position (kb) 225
226 Strain fitness (log2 ratio) -2
-1
0
1
2 at 224.249 kb on - strand at 224.294 kb on - strand at 224.364 kb on + strand at 224.707 kb on - strand, within Pf1N1B4_181 at 224.926 kb on + strand, within Pf1N1B4_181 at 224.927 kb on - strand, within Pf1N1B4_181 at 225.240 kb on + strand at 225.240 kb on + strand at 225.240 kb on + strand at 225.240 kb on + strand at 225.241 kb on - strand at 225.241 kb on - strand at 225.334 kb on + strand at 225.335 kb on - strand, within Pf1N1B4_182 at 225.558 kb on + strand at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand at 225.748 kb on - strand, within Pf1N1B4_182 at 225.748 kb on - strand, within Pf1N1B4_182 at 225.875 kb on - strand, within Pf1N1B4_182 at 225.980 kb on - strand at 225.980 kb on - strand at 225.989 kb on + strand at 226.236 kb on - strand, within Pf1N1B4_183 at 226.253 kb on + strand, within Pf1N1B4_183 at 226.494 kb on - strand, within Pf1N1B4_183 at 226.505 kb on + strand, within Pf1N1B4_183 at 226.505 kb on + strand, within Pf1N1B4_183 at 226.506 kb on - strand, within Pf1N1B4_183 at 226.506 kb on - strand, within Pf1N1B4_183 at 226.839 kb on - strand, within Pf1N1B4_183 at 226.839 kb on - strand at 226.960 kb on - strand, within Pf1N1B4_183
Per-strain Table
Position Strand Gene LocusTag Fraction benzoic 0.58 mg/ml remove 224,249 - -0.2 224,294 - +0.9 224,364 + +0.4 224,707 - Pf1N1B4_181 0.44 -0.2 224,926 + Pf1N1B4_181 0.67 -0.8 224,927 - Pf1N1B4_181 0.67 +2.1 225,240 + -0.4 225,240 + +0.2 225,240 + -0.6 225,240 + -1.8 225,241 - -0.8 225,241 - +1.1 225,334 + +0.5 225,335 - Pf1N1B4_182 0.14 -0.2 225,558 + +0.8 225,558 + Pf1N1B4_182 0.43 +1.1 225,558 + Pf1N1B4_182 0.43 -0.2 225,558 + Pf1N1B4_182 0.43 -0.8 225,558 + Pf1N1B4_182 0.43 -0.4 225,558 + Pf1N1B4_182 0.43 -1.2 225,559 - Pf1N1B4_182 0.44 +0.2 225,559 - Pf1N1B4_182 0.44 -1.2 225,559 - -0.2 225,559 - Pf1N1B4_182 0.44 -2.1 225,559 - +0.2 225,748 - Pf1N1B4_182 0.69 -1.7 225,748 - Pf1N1B4_182 0.69 -0.2 225,875 - Pf1N1B4_182 0.85 -2.4 225,980 - +0.5 225,980 - -1.2 225,989 + -0.8 226,236 - Pf1N1B4_183 0.20 +2.4 226,253 + Pf1N1B4_183 0.21 +0.0 226,494 - Pf1N1B4_183 0.39 -0.6 226,505 + Pf1N1B4_183 0.40 -0.8 226,505 + Pf1N1B4_183 0.40 +0.0 226,506 - Pf1N1B4_183 0.40 -0.5 226,506 - Pf1N1B4_183 0.40 -0.2 226,839 - Pf1N1B4_183 0.66 +0.8 226,839 - +2.4 226,960 - Pf1N1B4_183 0.75 -0.5
Or see this region's nucleotide sequence