Experiment: PYE with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02132 and CCNA_02133 are separated by 85 nucleotides CCNA_02133 and CCNA_02134 are separated by 14 nucleotides
CCNA_02132: CCNA_02132 - LacI-family transcriptional regulator, at 2,288,142 to 2,289,161
_02132
CCNA_02133: CCNA_02133 - glucokinase, at 2,289,247 to 2,290,242
_02133
CCNA_02134: CCNA_02134 - phosphogluconate dehydratase, at 2,290,257 to 2,292,065
_02134
Position (kb)
2289
2290
2291 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2288.305 kb on - strand, within CCNA_02132 at 2288.457 kb on - strand, within CCNA_02132 at 2288.502 kb on - strand at 2288.502 kb on - strand, within CCNA_02132 at 2288.561 kb on - strand, within CCNA_02132 at 2288.567 kb on - strand, within CCNA_02132 at 2288.572 kb on - strand, within CCNA_02132 at 2288.738 kb on - strand, within CCNA_02132 at 2288.800 kb on - strand, within CCNA_02132 at 2288.892 kb on - strand, within CCNA_02132 at 2288.921 kb on + strand, within CCNA_02132 at 2288.929 kb on - strand, within CCNA_02132 at 2289.008 kb on - strand, within CCNA_02132 at 2289.121 kb on - strand at 2289.131 kb on - strand at 2289.149 kb on - strand at 2289.201 kb on + strand at 2289.230 kb on - strand at 2289.273 kb on - strand at 2289.275 kb on - strand at 2289.498 kb on - strand, within CCNA_02133 at 2289.498 kb on - strand, within CCNA_02133 at 2289.546 kb on - strand, within CCNA_02133 at 2289.548 kb on - strand, within CCNA_02133 at 2289.602 kb on - strand, within CCNA_02133 at 2289.664 kb on - strand, within CCNA_02133 at 2289.774 kb on - strand, within CCNA_02133 at 2290.064 kb on - strand, within CCNA_02133 at 2290.101 kb on - strand, within CCNA_02133 at 2290.163 kb on + strand at 2290.171 kb on - strand at 2290.181 kb on - strand at 2290.216 kb on + strand at 2290.237 kb on + strand at 2290.252 kb on - strand at 2290.309 kb on + strand at 2290.317 kb on - strand at 2290.317 kb on - strand at 2290.436 kb on - strand at 2290.447 kb on + strand, within CCNA_02134 at 2290.448 kb on - strand, within CCNA_02134 at 2290.473 kb on - strand, within CCNA_02134 at 2290.524 kb on - strand, within CCNA_02134 at 2290.525 kb on - strand, within CCNA_02134 at 2290.534 kb on + strand, within CCNA_02134 at 2290.542 kb on - strand, within CCNA_02134 at 2290.593 kb on + strand, within CCNA_02134 at 2290.601 kb on - strand, within CCNA_02134 at 2290.645 kb on - strand, within CCNA_02134 at 2290.692 kb on - strand, within CCNA_02134 at 2290.731 kb on - strand, within CCNA_02134 at 2290.756 kb on - strand, within CCNA_02134 at 2290.855 kb on + strand, within CCNA_02134 at 2290.858 kb on - strand, within CCNA_02134 at 2290.858 kb on - strand, within CCNA_02134 at 2290.859 kb on - strand, within CCNA_02134 at 2290.862 kb on + strand, within CCNA_02134 at 2290.865 kb on - strand, within CCNA_02134 at 2290.953 kb on - strand, within CCNA_02134 at 2290.953 kb on - strand, within CCNA_02134 at 2290.953 kb on - strand, within CCNA_02134 at 2290.972 kb on + strand, within CCNA_02134 at 2290.972 kb on + strand, within CCNA_02134 at 2290.980 kb on - strand, within CCNA_02134 at 2291.022 kb on - strand, within CCNA_02134 at 2291.022 kb on - strand, within CCNA_02134 at 2291.104 kb on - strand, within CCNA_02134 at 2291.111 kb on - strand, within CCNA_02134 at 2291.155 kb on - strand, within CCNA_02134
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.025 mg/ml remove 2,288,305 - CCNA_02132 0.16 +0.5 2,288,457 - CCNA_02132 0.31 +1.9 2,288,502 - +1.3 2,288,502 - CCNA_02132 0.35 +0.5 2,288,561 - CCNA_02132 0.41 +2.1 2,288,567 - CCNA_02132 0.42 +0.6 2,288,572 - CCNA_02132 0.42 +0.8 2,288,738 - CCNA_02132 0.58 +0.8 2,288,800 - CCNA_02132 0.65 +1.2 2,288,892 - CCNA_02132 0.74 +2.6 2,288,921 + CCNA_02132 0.76 -1.0 2,288,929 - CCNA_02132 0.77 +1.3 2,289,008 - CCNA_02132 0.85 +1.9 2,289,121 - +2.5 2,289,131 - +1.7 2,289,149 - +1.4 2,289,201 + -1.3 2,289,230 - +0.4 2,289,273 - +1.5 2,289,275 - +0.7 2,289,498 - CCNA_02133 0.25 +1.1 2,289,498 - CCNA_02133 0.25 +1.4 2,289,546 - CCNA_02133 0.30 +1.6 2,289,548 - CCNA_02133 0.30 +1.7 2,289,602 - CCNA_02133 0.36 +2.0 2,289,664 - CCNA_02133 0.42 -0.4 2,289,774 - CCNA_02133 0.53 +2.0 2,290,064 - CCNA_02133 0.82 +1.9 2,290,101 - CCNA_02133 0.86 +2.3 2,290,163 + -1.4 2,290,171 - +1.9 2,290,181 - +1.9 2,290,216 + -0.9 2,290,237 + +1.1 2,290,252 - +0.5 2,290,309 + -1.7 2,290,317 - +1.7 2,290,317 - +0.7 2,290,436 - +1.3 2,290,447 + CCNA_02134 0.11 -2.6 2,290,448 - CCNA_02134 0.11 +0.9 2,290,473 - CCNA_02134 0.12 +0.2 2,290,524 - CCNA_02134 0.15 +1.4 2,290,525 - CCNA_02134 0.15 +1.2 2,290,534 + CCNA_02134 0.15 -0.3 2,290,542 - CCNA_02134 0.16 +1.1 2,290,593 + CCNA_02134 0.19 -1.5 2,290,601 - CCNA_02134 0.19 +1.0 2,290,645 - CCNA_02134 0.21 +1.0 2,290,692 - CCNA_02134 0.24 +1.0 2,290,731 - CCNA_02134 0.26 +1.3 2,290,756 - CCNA_02134 0.28 +1.4 2,290,855 + CCNA_02134 0.33 -0.9 2,290,858 - CCNA_02134 0.33 +0.3 2,290,858 - CCNA_02134 0.33 +0.8 2,290,859 - CCNA_02134 0.33 +1.3 2,290,862 + CCNA_02134 0.33 +0.3 2,290,865 - CCNA_02134 0.34 +1.3 2,290,953 - CCNA_02134 0.38 +1.7 2,290,953 - CCNA_02134 0.38 +0.8 2,290,953 - CCNA_02134 0.38 +1.0 2,290,972 + CCNA_02134 0.40 -2.0 2,290,972 + CCNA_02134 0.40 -1.8 2,290,980 - CCNA_02134 0.40 +0.1 2,291,022 - CCNA_02134 0.42 +1.2 2,291,022 - CCNA_02134 0.42 +1.3 2,291,104 - CCNA_02134 0.47 +1.4 2,291,111 - CCNA_02134 0.47 +1.0 2,291,155 - CCNA_02134 0.50 +1.1
Or see this region's nucleotide sequence