Experiment: PYE with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01941 and CCNA_01942 overlap by 4 nucleotides CCNA_01942 and CCNA_01943 are separated by 178 nucleotides CCNA_01943 and CCNA_01944 are separated by 66 nucleotides
CCNA_01941: CCNA_01941 - cysteine desulfhydrase/Selenocysteine lyase, at 2,083,785 to 2,084,924
_01941
CCNA_01942: CCNA_01942 - Rrf2 family protein, at 2,084,921 to 2,085,397
_01942
CCNA_01943: CCNA_01943 - alpha/beta hydrolase family protein, at 2,085,576 to 2,086,226
_01943
CCNA_01944: CCNA_01944 - McrA-family restriction endonuclease, at 2,086,293 to 2,086,811
_01944
Position (kb)
2084
2085
2086 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2084.925 kb on - strand at 2084.994 kb on - strand, within CCNA_01942 at 2084.997 kb on + strand, within CCNA_01942 at 2085.057 kb on + strand, within CCNA_01942 at 2085.072 kb on + strand, within CCNA_01942 at 2085.080 kb on - strand, within CCNA_01942 at 2085.092 kb on + strand, within CCNA_01942 at 2085.100 kb on - strand, within CCNA_01942 at 2085.101 kb on - strand, within CCNA_01942 at 2085.101 kb on - strand, within CCNA_01942 at 2085.101 kb on - strand, within CCNA_01942 at 2085.101 kb on - strand, within CCNA_01942 at 2085.167 kb on + strand, within CCNA_01942 at 2085.175 kb on - strand, within CCNA_01942 at 2085.182 kb on - strand, within CCNA_01942 at 2085.232 kb on - strand, within CCNA_01942 at 2085.273 kb on + strand, within CCNA_01942 at 2085.335 kb on - strand, within CCNA_01942 at 2085.339 kb on + strand, within CCNA_01942 at 2085.400 kb on + strand at 2085.400 kb on + strand at 2085.400 kb on + strand at 2085.400 kb on + strand at 2085.400 kb on + strand at 2085.400 kb on + strand at 2085.400 kb on + strand at 2085.400 kb on + strand at 2085.400 kb on + strand at 2085.400 kb on + strand at 2085.407 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.408 kb on - strand at 2085.451 kb on - strand at 2086.025 kb on + strand, within CCNA_01943 at 2086.234 kb on + strand at 2086.242 kb on - strand at 2086.244 kb on + strand at 2086.244 kb on + strand at 2086.244 kb on + strand at 2086.252 kb on - strand at 2086.256 kb on + strand at 2086.291 kb on + strand at 2086.299 kb on - strand at 2086.299 kb on - strand at 2086.346 kb on - strand, within CCNA_01944 at 2086.361 kb on - strand, within CCNA_01944
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.025 mg/ml remove 2,084,925 - +0.7 2,084,994 - CCNA_01942 0.15 +1.0 2,084,997 + CCNA_01942 0.16 -1.6 2,085,057 + CCNA_01942 0.29 -0.0 2,085,072 + CCNA_01942 0.32 +2.4 2,085,080 - CCNA_01942 0.33 +1.1 2,085,092 + CCNA_01942 0.36 +2.5 2,085,100 - CCNA_01942 0.38 +1.7 2,085,101 - CCNA_01942 0.38 +1.6 2,085,101 - CCNA_01942 0.38 +2.0 2,085,101 - CCNA_01942 0.38 +1.0 2,085,101 - CCNA_01942 0.38 +2.0 2,085,167 + CCNA_01942 0.52 +0.5 2,085,175 - CCNA_01942 0.53 +1.8 2,085,182 - CCNA_01942 0.55 +1.6 2,085,232 - CCNA_01942 0.65 +1.9 2,085,273 + CCNA_01942 0.74 +2.3 2,085,335 - CCNA_01942 0.87 +1.2 2,085,339 + CCNA_01942 0.88 -0.1 2,085,400 + -0.5 2,085,400 + -1.2 2,085,400 + -0.5 2,085,400 + -1.3 2,085,400 + -0.8 2,085,400 + -0.3 2,085,400 + +0.1 2,085,400 + -0.4 2,085,400 + -1.1 2,085,400 + -2.2 2,085,407 - +0.4 2,085,408 - -0.9 2,085,408 - -0.6 2,085,408 - +0.8 2,085,408 - -0.7 2,085,408 - +0.3 2,085,408 - -1.3 2,085,408 - -0.7 2,085,408 - +0.2 2,085,408 - +0.5 2,085,408 - +1.4 2,085,408 - +1.7 2,085,408 - -0.3 2,085,408 - +0.7 2,085,408 - -0.6 2,085,408 - +0.2 2,085,451 - +1.4 2,086,025 + CCNA_01943 0.69 +0.7 2,086,234 + +1.6 2,086,242 - -0.1 2,086,244 + +1.0 2,086,244 + +1.8 2,086,244 + +1.5 2,086,252 - -0.3 2,086,256 + -0.8 2,086,291 + -0.8 2,086,299 - -1.0 2,086,299 - +0.5 2,086,346 - CCNA_01944 0.10 +1.3 2,086,361 - CCNA_01944 0.13 +0.8
Or see this region's nucleotide sequence