Experiment: PYE with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01291 and CCNA_01292 overlap by 4 nucleotides CCNA_01292 and CCNA_01293 are separated by 60 nucleotides CCNA_01293 and CCNA_01294 overlap by 43 nucleotides CCNA_01294 and CCNA_01295 are separated by 29 nucleotides
CCNA_01291: CCNA_01291 - phosphotransferase family protein, at 1,416,848 to 1,417,858
_01291
CCNA_01292: CCNA_01292 - hypothetical protein, at 1,417,855 to 1,418,280
_01292
CCNA_01293: CCNA_01293 - hypothetical protein, at 1,418,341 to 1,418,772
_01293
CCNA_01294: CCNA_01294 - transposase, at 1,418,730 to 1,419,224
_01294
CCNA_01295: CCNA_01295 - integral membrane protein, at 1,419,254 to 1,419,994
_01295
Position (kb)
1418
1419 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1417.341 kb on + strand, within CCNA_01291 at 1417.392 kb on - strand, within CCNA_01291 at 1417.437 kb on - strand at 1417.437 kb on - strand, within CCNA_01291 at 1417.493 kb on + strand, within CCNA_01291 at 1417.493 kb on + strand, within CCNA_01291 at 1417.501 kb on - strand, within CCNA_01291 at 1417.526 kb on - strand, within CCNA_01291 at 1417.550 kb on + strand, within CCNA_01291 at 1417.558 kb on - strand, within CCNA_01291 at 1417.582 kb on - strand, within CCNA_01291 at 1417.628 kb on + strand, within CCNA_01291 at 1417.628 kb on + strand, within CCNA_01291 at 1417.636 kb on - strand, within CCNA_01291 at 1417.649 kb on - strand, within CCNA_01291 at 1417.676 kb on + strand, within CCNA_01291 at 1417.676 kb on + strand, within CCNA_01291 at 1417.676 kb on + strand, within CCNA_01291 at 1417.676 kb on + strand, within CCNA_01291 at 1417.676 kb on + strand, within CCNA_01291 at 1417.684 kb on - strand, within CCNA_01291 at 1417.684 kb on - strand, within CCNA_01291 at 1417.684 kb on - strand, within CCNA_01291 at 1417.684 kb on - strand, within CCNA_01291 at 1417.684 kb on - strand, within CCNA_01291 at 1417.684 kb on - strand, within CCNA_01291 at 1417.684 kb on - strand, within CCNA_01291 at 1417.704 kb on - strand, within CCNA_01291 at 1417.711 kb on + strand, within CCNA_01291 at 1417.719 kb on - strand, within CCNA_01291 at 1417.767 kb on + strand at 1417.774 kb on - strand at 1417.781 kb on + strand at 1417.795 kb on - strand at 1417.797 kb on - strand at 1417.816 kb on + strand at 1417.820 kb on - strand at 1417.904 kb on - strand, within CCNA_01292 at 1417.904 kb on - strand, within CCNA_01292 at 1417.904 kb on - strand, within CCNA_01292 at 1417.904 kb on - strand, within CCNA_01292 at 1417.907 kb on + strand, within CCNA_01292 at 1417.907 kb on + strand, within CCNA_01292 at 1417.929 kb on + strand, within CCNA_01292 at 1418.066 kb on + strand, within CCNA_01292 at 1418.067 kb on - strand, within CCNA_01292 at 1418.073 kb on + strand, within CCNA_01292 at 1418.074 kb on - strand, within CCNA_01292 at 1418.074 kb on - strand, within CCNA_01292 at 1418.148 kb on - strand, within CCNA_01292 at 1418.313 kb on - strand at 1419.268 kb on - strand at 1419.269 kb on - strand at 1419.277 kb on - strand at 1419.287 kb on - strand at 1419.338 kb on - strand, within CCNA_01295 at 1419.346 kb on - strand, within CCNA_01295 at 1419.346 kb on - strand, within CCNA_01295 at 1419.372 kb on - strand, within CCNA_01295 at 1419.395 kb on - strand, within CCNA_01295 at 1419.404 kb on + strand, within CCNA_01295 at 1419.504 kb on - strand, within CCNA_01295 at 1419.600 kb on + strand, within CCNA_01295 at 1419.600 kb on + strand, within CCNA_01295 at 1419.608 kb on - strand, within CCNA_01295 at 1419.616 kb on - strand, within CCNA_01295 at 1419.622 kb on - strand, within CCNA_01295 at 1419.629 kb on + strand at 1419.631 kb on + strand, within CCNA_01295 at 1419.639 kb on - strand, within CCNA_01295 at 1419.646 kb on - strand, within CCNA_01295 at 1419.646 kb on - strand, within CCNA_01295 at 1419.660 kb on + strand, within CCNA_01295 at 1419.666 kb on + strand, within CCNA_01295 at 1419.681 kb on + strand, within CCNA_01295 at 1419.681 kb on + strand, within CCNA_01295 at 1419.712 kb on + strand, within CCNA_01295 at 1419.720 kb on - strand, within CCNA_01295 at 1419.720 kb on - strand, within CCNA_01295 at 1419.722 kb on + strand, within CCNA_01295
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.025 mg/ml remove 1,417,341 + CCNA_01291 0.49 +0.0 1,417,392 - CCNA_01291 0.54 +0.8 1,417,437 - +0.4 1,417,437 - CCNA_01291 0.58 +0.2 1,417,493 + CCNA_01291 0.64 -0.3 1,417,493 + CCNA_01291 0.64 +1.2 1,417,501 - CCNA_01291 0.65 -3.2 1,417,526 - CCNA_01291 0.67 +1.2 1,417,550 + CCNA_01291 0.69 -0.1 1,417,558 - CCNA_01291 0.70 +0.0 1,417,582 - CCNA_01291 0.73 +1.6 1,417,628 + CCNA_01291 0.77 +0.1 1,417,628 + CCNA_01291 0.77 -0.8 1,417,636 - CCNA_01291 0.78 -2.7 1,417,649 - CCNA_01291 0.79 -1.1 1,417,676 + CCNA_01291 0.82 -0.8 1,417,676 + CCNA_01291 0.82 +0.4 1,417,676 + CCNA_01291 0.82 -2.2 1,417,676 + CCNA_01291 0.82 +0.0 1,417,676 + CCNA_01291 0.82 -1.0 1,417,684 - CCNA_01291 0.83 -0.3 1,417,684 - CCNA_01291 0.83 +0.4 1,417,684 - CCNA_01291 0.83 +0.4 1,417,684 - CCNA_01291 0.83 -1.1 1,417,684 - CCNA_01291 0.83 -0.0 1,417,684 - CCNA_01291 0.83 -1.2 1,417,684 - CCNA_01291 0.83 +2.2 1,417,704 - CCNA_01291 0.85 +0.6 1,417,711 + CCNA_01291 0.85 +0.7 1,417,719 - CCNA_01291 0.86 -1.6 1,417,767 + -0.8 1,417,774 - -1.1 1,417,781 + +0.5 1,417,795 - +0.4 1,417,797 - +0.7 1,417,816 + -1.1 1,417,820 - -0.9 1,417,904 - CCNA_01292 0.12 +0.9 1,417,904 - CCNA_01292 0.12 -0.3 1,417,904 - CCNA_01292 0.12 +0.4 1,417,904 - CCNA_01292 0.12 +0.6 1,417,907 + CCNA_01292 0.12 -0.4 1,417,907 + CCNA_01292 0.12 -0.7 1,417,929 + CCNA_01292 0.17 -2.2 1,418,066 + CCNA_01292 0.50 +0.0 1,418,067 - CCNA_01292 0.50 +0.1 1,418,073 + CCNA_01292 0.51 -0.6 1,418,074 - CCNA_01292 0.51 +0.0 1,418,074 - CCNA_01292 0.51 +0.2 1,418,148 - CCNA_01292 0.69 -1.8 1,418,313 - -1.4 1,419,268 - +0.9 1,419,269 - +1.6 1,419,277 - +1.4 1,419,287 - +1.6 1,419,338 - CCNA_01295 0.11 +0.7 1,419,346 - CCNA_01295 0.12 +1.0 1,419,346 - CCNA_01295 0.12 +1.2 1,419,372 - CCNA_01295 0.16 +2.0 1,419,395 - CCNA_01295 0.19 -1.6 1,419,404 + CCNA_01295 0.20 +0.4 1,419,504 - CCNA_01295 0.34 +2.9 1,419,600 + CCNA_01295 0.47 +1.4 1,419,600 + CCNA_01295 0.47 +1.2 1,419,608 - CCNA_01295 0.48 +2.6 1,419,616 - CCNA_01295 0.49 +2.7 1,419,622 - CCNA_01295 0.50 +2.9 1,419,629 + +2.6 1,419,631 + CCNA_01295 0.51 +0.8 1,419,639 - CCNA_01295 0.52 +2.2 1,419,646 - CCNA_01295 0.53 +2.7 1,419,646 - CCNA_01295 0.53 +2.8 1,419,660 + CCNA_01295 0.55 +2.0 1,419,666 + CCNA_01295 0.56 +2.3 1,419,681 + CCNA_01295 0.58 +1.8 1,419,681 + CCNA_01295 0.58 +2.0 1,419,712 + CCNA_01295 0.62 +2.0 1,419,720 - CCNA_01295 0.63 +1.7 1,419,720 - CCNA_01295 0.63 +3.8 1,419,722 + CCNA_01295 0.63 +1.6
Or see this region's nucleotide sequence