Experiment: PYE with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00565 and CCNA_R0008 are separated by 253 nucleotides CCNA_R0008 and CCNA_00566 are separated by 221 nucleotides CCNA_00566 and CCNA_00567 are separated by 122 nucleotides
CCNA_00565: CCNA_00565 - gamma-glutamyltranspeptidase, at 580,036 to 581,778
_00565
CCNA_R0008: CCNA_R0008 - tRNA-Arg, at 582,032 to 582,108
_R0008
CCNA_00566: CCNA_00566 - mannonate dehydratase, at 582,330 to 583,541
_00566
CCNA_00567: CCNA_00567 - alpha-1,2-mannosidase, at 583,664 to 585,976
_00567
Position (kb) 582
583
584 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 581.378 kb on - strand, within CCNA_00565 at 581.378 kb on - strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.380 kb on + strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.388 kb on - strand, within CCNA_00565 at 581.389 kb on - strand, within CCNA_00565 at 581.395 kb on - strand, within CCNA_00565 at 581.395 kb on - strand, within CCNA_00565 at 581.401 kb on - strand, within CCNA_00565 at 581.446 kb on - strand, within CCNA_00565 at 581.449 kb on + strand, within CCNA_00565 at 581.461 kb on + strand, within CCNA_00565 at 581.471 kb on + strand, within CCNA_00565 at 581.506 kb on + strand, within CCNA_00565 at 581.511 kb on + strand, within CCNA_00565 at 581.511 kb on + strand, within CCNA_00565 at 581.514 kb on - strand, within CCNA_00565 at 581.514 kb on - strand, within CCNA_00565 at 581.514 kb on - strand, within CCNA_00565 at 581.514 kb on - strand, within CCNA_00565 at 581.519 kb on - strand, within CCNA_00565 at 581.544 kb on - strand, within CCNA_00565 at 581.601 kb on + strand, within CCNA_00565 at 581.604 kb on - strand at 581.641 kb on + strand at 581.652 kb on + strand at 581.652 kb on + strand at 581.653 kb on + strand at 581.660 kb on - strand at 581.699 kb on + strand at 581.721 kb on + strand at 581.731 kb on + strand at 581.739 kb on + strand at 581.773 kb on - strand at 581.854 kb on - strand at 581.865 kb on - strand at 581.884 kb on + strand at 581.884 kb on + strand at 581.911 kb on + strand at 581.911 kb on + strand at 582.222 kb on - strand at 582.224 kb on + strand at 582.224 kb on + strand at 582.236 kb on - strand at 582.257 kb on - strand at 582.295 kb on + strand at 582.297 kb on + strand at 582.300 kb on + strand at 582.308 kb on - strand at 582.336 kb on + strand at 582.336 kb on + strand at 582.359 kb on - strand at 582.382 kb on - strand at 582.385 kb on + strand at 582.400 kb on + strand at 582.402 kb on + strand at 582.407 kb on + strand at 582.431 kb on + strand at 582.439 kb on - strand at 582.453 kb on + strand, within CCNA_00566 at 582.461 kb on - strand, within CCNA_00566 at 582.472 kb on + strand, within CCNA_00566 at 582.478 kb on - strand, within CCNA_00566 at 582.479 kb on - strand, within CCNA_00566 at 582.480 kb on - strand, within CCNA_00566 at 582.480 kb on - strand, within CCNA_00566 at 582.516 kb on + strand, within CCNA_00566 at 582.558 kb on + strand, within CCNA_00566 at 582.566 kb on - strand, within CCNA_00566 at 582.566 kb on - strand, within CCNA_00566 at 582.566 kb on - strand, within CCNA_00566 at 582.566 kb on - strand, within CCNA_00566 at 582.579 kb on - strand, within CCNA_00566 at 582.598 kb on - strand, within CCNA_00566 at 582.598 kb on - strand, within CCNA_00566 at 582.612 kb on + strand, within CCNA_00566 at 582.613 kb on + strand, within CCNA_00566 at 582.621 kb on - strand, within CCNA_00566 at 582.623 kb on + strand, within CCNA_00566 at 582.624 kb on + strand, within CCNA_00566 at 582.632 kb on - strand, within CCNA_00566 at 582.664 kb on - strand, within CCNA_00566 at 582.667 kb on + strand, within CCNA_00566 at 582.675 kb on + strand, within CCNA_00566 at 582.680 kb on + strand, within CCNA_00566 at 582.680 kb on + strand, within CCNA_00566 at 582.683 kb on - strand, within CCNA_00566 at 582.688 kb on - strand, within CCNA_00566 at 582.733 kb on + strand, within CCNA_00566 at 582.733 kb on + strand, within CCNA_00566 at 582.733 kb on + strand, within CCNA_00566 at 582.741 kb on - strand, within CCNA_00566 at 582.741 kb on - strand, within CCNA_00566 at 582.768 kb on + strand, within CCNA_00566 at 582.778 kb on - strand, within CCNA_00566 at 582.778 kb on - strand, within CCNA_00566 at 582.861 kb on - strand, within CCNA_00566 at 582.888 kb on - strand, within CCNA_00566 at 582.890 kb on + strand, within CCNA_00566 at 582.900 kb on + strand, within CCNA_00566 at 582.900 kb on + strand, within CCNA_00566 at 582.900 kb on - strand, within CCNA_00566 at 582.926 kb on + strand, within CCNA_00566 at 582.928 kb on + strand, within CCNA_00566 at 582.928 kb on + strand, within CCNA_00566 at 582.933 kb on + strand, within CCNA_00566 at 582.933 kb on + strand, within CCNA_00566 at 582.936 kb on - strand, within CCNA_00566 at 582.936 kb on - strand, within CCNA_00566 at 582.936 kb on - strand, within CCNA_00566 at 582.941 kb on - strand, within CCNA_00566 at 582.941 kb on - strand, within CCNA_00566 at 582.952 kb on + strand, within CCNA_00566 at 582.952 kb on + strand, within CCNA_00566 at 582.952 kb on + strand, within CCNA_00566 at 582.952 kb on + strand, within CCNA_00566 at 582.960 kb on - strand, within CCNA_00566 at 582.962 kb on + strand, within CCNA_00566 at 582.962 kb on + strand, within CCNA_00566 at 582.962 kb on + strand, within CCNA_00566 at 582.962 kb on + strand, within CCNA_00566 at 582.962 kb on + strand, within CCNA_00566 at 582.962 kb on + strand, within CCNA_00566 at 582.962 kb on + strand, within CCNA_00566 at 583.002 kb on - strand, within CCNA_00566 at 583.060 kb on + strand, within CCNA_00566 at 583.066 kb on - strand, within CCNA_00566 at 583.066 kb on - strand, within CCNA_00566 at 583.071 kb on + strand, within CCNA_00566 at 583.074 kb on - strand, within CCNA_00566 at 583.120 kb on + strand, within CCNA_00566 at 583.120 kb on + strand, within CCNA_00566 at 583.120 kb on + strand, within CCNA_00566 at 583.120 kb on + strand, within CCNA_00566 at 583.120 kb on + strand, within CCNA_00566 at 583.120 kb on + strand, within CCNA_00566 at 583.128 kb on - strand, within CCNA_00566 at 583.128 kb on - strand, within CCNA_00566 at 583.128 kb on - strand, within CCNA_00566 at 583.128 kb on - strand, within CCNA_00566 at 583.128 kb on - strand, within CCNA_00566 at 583.128 kb on - strand, within CCNA_00566 at 583.128 kb on - strand, within CCNA_00566 at 583.128 kb on - strand, within CCNA_00566 at 583.141 kb on + strand, within CCNA_00566 at 583.149 kb on - strand, within CCNA_00566 at 583.157 kb on - strand, within CCNA_00566 at 583.184 kb on - strand, within CCNA_00566 at 583.232 kb on + strand, within CCNA_00566 at 583.239 kb on + strand, within CCNA_00566 at 583.248 kb on - strand, within CCNA_00566 at 583.260 kb on - strand, within CCNA_00566 at 583.270 kb on - strand, within CCNA_00566 at 583.288 kb on + strand, within CCNA_00566 at 583.313 kb on + strand, within CCNA_00566 at 583.345 kb on + strand, within CCNA_00566 at 583.353 kb on - strand, within CCNA_00566 at 583.428 kb on + strand at 583.433 kb on + strand at 583.436 kb on - strand at 583.483 kb on - strand at 583.483 kb on - strand at 583.509 kb on - strand at 583.510 kb on + strand at 583.510 kb on + strand at 583.543 kb on + strand at 583.543 kb on + strand at 583.543 kb on + strand at 583.680 kb on + strand at 583.712 kb on - strand at 583.725 kb on - strand at 583.732 kb on - strand at 583.773 kb on - strand at 583.775 kb on - strand at 583.796 kb on + strand at 583.806 kb on - strand at 583.820 kb on - strand at 583.828 kb on - strand at 583.828 kb on - strand at 583.876 kb on - strand at 583.895 kb on + strand at 583.936 kb on + strand, within CCNA_00567 at 583.937 kb on - strand, within CCNA_00567 at 583.942 kb on + strand, within CCNA_00567 at 583.943 kb on - strand, within CCNA_00567 at 583.975 kb on - strand, within CCNA_00567 at 583.977 kb on + strand, within CCNA_00567 at 583.985 kb on - strand, within CCNA_00567 at 584.012 kb on + strand, within CCNA_00567 at 584.058 kb on - strand, within CCNA_00567 at 584.094 kb on + strand, within CCNA_00567 at 584.133 kb on + strand, within CCNA_00567 at 584.136 kb on + strand, within CCNA_00567 at 584.167 kb on + strand, within CCNA_00567 at 584.167 kb on + strand, within CCNA_00567 at 584.225 kb on + strand, within CCNA_00567 at 584.225 kb on - strand, within CCNA_00567 at 584.244 kb on + strand, within CCNA_00567 at 584.249 kb on + strand, within CCNA_00567 at 584.269 kb on + strand, within CCNA_00567 at 584.283 kb on + strand, within CCNA_00567 at 584.288 kb on + strand, within CCNA_00567 at 584.298 kb on + strand, within CCNA_00567 at 584.338 kb on + strand, within CCNA_00567 at 584.369 kb on - strand, within CCNA_00567 at 584.397 kb on - strand, within CCNA_00567 at 584.423 kb on - strand, within CCNA_00567 at 584.448 kb on - strand, within CCNA_00567 at 584.453 kb on + strand, within CCNA_00567 at 584.463 kb on - strand, within CCNA_00567 at 584.467 kb on + strand, within CCNA_00567 at 584.467 kb on + strand, within CCNA_00567 at 584.471 kb on - strand, within CCNA_00567 at 584.475 kb on - strand, within CCNA_00567 at 584.475 kb on - strand, within CCNA_00567 at 584.485 kb on - strand, within CCNA_00567 at 584.506 kb on + strand, within CCNA_00567 at 584.518 kb on + strand, within CCNA_00567 at 584.518 kb on + strand, within CCNA_00567 at 584.523 kb on + strand, within CCNA_00567 at 584.526 kb on - strand, within CCNA_00567 at 584.526 kb on - strand, within CCNA_00567
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.025 mg/ml remove 581,378 - CCNA_00565 0.77 +0.7 581,378 - CCNA_00565 0.77 +0.6 581,380 + CCNA_00565 0.77 -1.2 581,380 + CCNA_00565 0.77 -2.1 581,380 + CCNA_00565 0.77 +1.9 581,380 + CCNA_00565 0.77 +1.7 581,380 + CCNA_00565 0.77 -1.4 581,380 + CCNA_00565 0.77 +0.9 581,380 + CCNA_00565 0.77 -0.7 581,380 + CCNA_00565 0.77 +0.7 581,380 + CCNA_00565 0.77 -1.3 581,380 + CCNA_00565 0.77 -0.4 581,380 + CCNA_00565 0.77 -0.1 581,380 + CCNA_00565 0.77 -0.5 581,388 - CCNA_00565 0.78 -0.4 581,388 - CCNA_00565 0.78 +0.7 581,388 - CCNA_00565 0.78 +0.4 581,388 - CCNA_00565 0.78 +0.9 581,388 - CCNA_00565 0.78 +0.4 581,388 - CCNA_00565 0.78 -0.3 581,388 - CCNA_00565 0.78 +1.1 581,388 - CCNA_00565 0.78 -0.4 581,388 - CCNA_00565 0.78 +0.9 581,388 - CCNA_00565 0.78 +0.7 581,388 - CCNA_00565 0.78 +1.1 581,388 - CCNA_00565 0.78 +1.0 581,388 - CCNA_00565 0.78 +1.0 581,389 - CCNA_00565 0.78 +0.7 581,395 - CCNA_00565 0.78 +0.7 581,395 - CCNA_00565 0.78 +0.8 581,401 - CCNA_00565 0.78 +0.2 581,446 - CCNA_00565 0.81 -0.6 581,449 + CCNA_00565 0.81 -0.1 581,461 + CCNA_00565 0.82 -1.0 581,471 + CCNA_00565 0.82 +1.2 581,506 + CCNA_00565 0.84 +1.3 581,511 + CCNA_00565 0.85 -1.5 581,511 + CCNA_00565 0.85 -0.4 581,514 - CCNA_00565 0.85 +1.3 581,514 - CCNA_00565 0.85 +0.6 581,514 - CCNA_00565 0.85 +0.5 581,514 - CCNA_00565 0.85 -0.0 581,519 - CCNA_00565 0.85 -1.4 581,544 - CCNA_00565 0.87 +0.6 581,601 + CCNA_00565 0.90 -0.1 581,604 - -0.5 581,641 + +0.9 581,652 + -0.1 581,652 + +1.2 581,653 + +2.1 581,660 - -0.2 581,699 + -0.7 581,721 + -0.1 581,731 + -0.6 581,739 + +0.1 581,773 - +1.0 581,854 - +1.0 581,865 - +0.0 581,884 + -0.1 581,884 + -1.8 581,911 + -1.2 581,911 + -1.8 582,222 - -2.5 582,224 + -0.4 582,224 + -0.5 582,236 - -0.9 582,257 - +0.0 582,295 + +0.2 582,297 + -0.5 582,300 + -0.5 582,308 - -1.0 582,336 + +0.5 582,336 + +0.2 582,359 - +0.6 582,382 - -0.3 582,385 + -0.6 582,400 + +1.2 582,402 + -0.6 582,407 + -0.7 582,431 + +0.0 582,439 - +0.0 582,453 + CCNA_00566 0.10 -0.3 582,461 - CCNA_00566 0.11 +1.3 582,472 + CCNA_00566 0.12 -1.5 582,478 - CCNA_00566 0.12 -0.6 582,479 - CCNA_00566 0.12 -2.2 582,480 - CCNA_00566 0.12 -1.8 582,480 - CCNA_00566 0.12 -0.5 582,516 + CCNA_00566 0.15 -2.1 582,558 + CCNA_00566 0.19 -2.1 582,566 - CCNA_00566 0.19 +0.3 582,566 - CCNA_00566 0.19 -0.5 582,566 - CCNA_00566 0.19 +1.1 582,566 - CCNA_00566 0.19 +1.1 582,579 - CCNA_00566 0.21 -0.0 582,598 - CCNA_00566 0.22 -1.2 582,598 - CCNA_00566 0.22 +1.5 582,612 + CCNA_00566 0.23 -0.3 582,613 + CCNA_00566 0.23 -1.8 582,621 - CCNA_00566 0.24 -2.5 582,623 + CCNA_00566 0.24 +1.7 582,624 + CCNA_00566 0.24 +0.1 582,632 - CCNA_00566 0.25 -0.8 582,664 - CCNA_00566 0.28 +1.2 582,667 + CCNA_00566 0.28 -2.5 582,675 + CCNA_00566 0.28 +0.3 582,680 + CCNA_00566 0.29 -0.7 582,680 + CCNA_00566 0.29 -1.3 582,683 - CCNA_00566 0.29 +0.3 582,688 - CCNA_00566 0.30 +0.2 582,733 + CCNA_00566 0.33 +0.7 582,733 + CCNA_00566 0.33 +0.1 582,733 + CCNA_00566 0.33 -0.2 582,741 - CCNA_00566 0.34 -0.4 582,741 - CCNA_00566 0.34 -1.8 582,768 + CCNA_00566 0.36 -1.3 582,778 - CCNA_00566 0.37 -0.1 582,778 - CCNA_00566 0.37 -0.6 582,861 - CCNA_00566 0.44 -1.9 582,888 - CCNA_00566 0.46 -2.3 582,890 + CCNA_00566 0.46 -0.1 582,900 + CCNA_00566 0.47 -0.2 582,900 + CCNA_00566 0.47 -1.4 582,900 - CCNA_00566 0.47 -0.9 582,926 + CCNA_00566 0.49 +0.6 582,928 + CCNA_00566 0.49 +0.2 582,928 + CCNA_00566 0.49 +0.1 582,933 + CCNA_00566 0.50 -0.3 582,933 + CCNA_00566 0.50 -0.4 582,936 - CCNA_00566 0.50 -0.3 582,936 - CCNA_00566 0.50 +0.5 582,936 - CCNA_00566 0.50 -1.2 582,941 - CCNA_00566 0.50 -0.1 582,941 - CCNA_00566 0.50 +0.7 582,952 + CCNA_00566 0.51 -0.5 582,952 + CCNA_00566 0.51 -1.1 582,952 + CCNA_00566 0.51 -2.2 582,952 + CCNA_00566 0.51 +0.3 582,960 - CCNA_00566 0.52 -0.1 582,962 + CCNA_00566 0.52 -2.0 582,962 + CCNA_00566 0.52 -1.6 582,962 + CCNA_00566 0.52 -0.5 582,962 + CCNA_00566 0.52 -1.3 582,962 + CCNA_00566 0.52 +1.1 582,962 + CCNA_00566 0.52 -0.3 582,962 + CCNA_00566 0.52 +0.3 583,002 - CCNA_00566 0.55 -1.8 583,060 + CCNA_00566 0.60 -0.9 583,066 - CCNA_00566 0.61 -0.5 583,066 - CCNA_00566 0.61 -1.5 583,071 + CCNA_00566 0.61 +0.5 583,074 - CCNA_00566 0.61 -2.1 583,120 + CCNA_00566 0.65 -1.1 583,120 + CCNA_00566 0.65 +0.0 583,120 + CCNA_00566 0.65 +1.0 583,120 + CCNA_00566 0.65 -1.3 583,120 + CCNA_00566 0.65 -0.7 583,120 + CCNA_00566 0.65 -0.3 583,128 - CCNA_00566 0.66 -1.4 583,128 - CCNA_00566 0.66 -1.6 583,128 - CCNA_00566 0.66 -0.5 583,128 - CCNA_00566 0.66 -0.7 583,128 - CCNA_00566 0.66 -0.4 583,128 - CCNA_00566 0.66 -0.1 583,128 - CCNA_00566 0.66 -0.1 583,128 - CCNA_00566 0.66 +0.6 583,141 + CCNA_00566 0.67 +1.0 583,149 - CCNA_00566 0.68 +0.7 583,157 - CCNA_00566 0.68 +0.0 583,184 - CCNA_00566 0.70 -0.5 583,232 + CCNA_00566 0.74 -0.4 583,239 + CCNA_00566 0.75 +0.0 583,248 - CCNA_00566 0.76 -0.6 583,260 - CCNA_00566 0.77 -0.5 583,270 - CCNA_00566 0.78 +0.0 583,288 + CCNA_00566 0.79 -1.3 583,313 + CCNA_00566 0.81 -0.8 583,345 + CCNA_00566 0.84 +0.1 583,353 - CCNA_00566 0.84 -0.4 583,428 + -1.9 583,433 + -0.5 583,436 - -2.8 583,483 - -1.1 583,483 - -2.1 583,509 - +0.0 583,510 + -0.2 583,510 + +0.7 583,543 + -1.2 583,543 + -0.4 583,543 + +0.2 583,680 + -0.4 583,712 - -0.7 583,725 - -0.7 583,732 - -1.2 583,773 - +0.5 583,775 - -0.9 583,796 + +0.2 583,806 - -0.3 583,820 - +1.5 583,828 - -0.1 583,828 - -0.1 583,876 - -1.2 583,895 + -0.4 583,936 + CCNA_00567 0.12 -1.0 583,937 - CCNA_00567 0.12 -0.5 583,942 + CCNA_00567 0.12 -0.8 583,943 - CCNA_00567 0.12 +0.5 583,975 - CCNA_00567 0.13 -1.2 583,977 + CCNA_00567 0.14 +0.7 583,985 - CCNA_00567 0.14 -1.7 584,012 + CCNA_00567 0.15 -0.6 584,058 - CCNA_00567 0.17 -1.3 584,094 + CCNA_00567 0.19 -3.2 584,133 + CCNA_00567 0.20 -0.5 584,136 + CCNA_00567 0.20 -2.5 584,167 + CCNA_00567 0.22 +1.1 584,167 + CCNA_00567 0.22 +0.1 584,225 + CCNA_00567 0.24 -0.4 584,225 - CCNA_00567 0.24 -0.6 584,244 + CCNA_00567 0.25 -0.1 584,249 + CCNA_00567 0.25 +0.0 584,269 + CCNA_00567 0.26 -0.4 584,283 + CCNA_00567 0.27 -0.7 584,288 + CCNA_00567 0.27 -0.6 584,298 + CCNA_00567 0.27 -0.7 584,338 + CCNA_00567 0.29 -0.8 584,369 - CCNA_00567 0.30 +0.5 584,397 - CCNA_00567 0.32 -0.7 584,423 - CCNA_00567 0.33 -0.6 584,448 - CCNA_00567 0.34 -1.0 584,453 + CCNA_00567 0.34 -0.9 584,463 - CCNA_00567 0.35 -0.4 584,467 + CCNA_00567 0.35 -0.5 584,467 + CCNA_00567 0.35 -0.8 584,471 - CCNA_00567 0.35 -0.6 584,475 - CCNA_00567 0.35 +0.5 584,475 - CCNA_00567 0.35 +0.2 584,485 - CCNA_00567 0.35 -1.6 584,506 + CCNA_00567 0.36 +0.3 584,518 + CCNA_00567 0.37 -0.4 584,518 + CCNA_00567 0.37 -2.0 584,523 + CCNA_00567 0.37 -2.1 584,526 - CCNA_00567 0.37 -0.6 584,526 - CCNA_00567 0.37 -2.1
Or see this region's nucleotide sequence