Experiment: root sample 5; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS04455 and ABZR87_RS04460 are separated by 372 nucleotides ABZR87_RS04460 and ABZR87_RS04465 are separated by 48 nucleotides ABZR87_RS04465 and ABZR87_RS04470 are separated by 5 nucleotides ABZR87_RS04470 and ABZR87_RS04475 are separated by 494 nucleotides
ABZR87_RS04455: ABZR87_RS04455 - alpha/beta hydrolase, at 931,969 to 932,877
_RS04455
ABZR87_RS04460: ABZR87_RS04460 - hypothetical protein, at 933,250 to 933,555
_RS04460
ABZR87_RS04465: ABZR87_RS04465 - hypothetical protein, at 933,604 to 933,933
_RS04465
ABZR87_RS04470: ABZR87_RS04470 - hypothetical protein, at 933,939 to 934,253
_RS04470
ABZR87_RS04475: ABZR87_RS04475 - hypothetical protein, at 934,748 to 935,110
_RS04475
Position (kb) 933
934 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9
10 at 932.605 kb on + strand, within ABZR87_RS04455 at 932.841 kb on + strand at 932.883 kb on + strand at 932.883 kb on + strand at 932.883 kb on + strand at 932.884 kb on - strand at 932.884 kb on - strand at 932.884 kb on - strand at 932.906 kb on + strand at 932.906 kb on + strand at 932.906 kb on + strand at 932.939 kb on + strand at 932.939 kb on + strand at 932.940 kb on - strand at 932.940 kb on - strand at 932.990 kb on + strand at 932.993 kb on + strand at 932.993 kb on + strand at 932.993 kb on + strand at 932.994 kb on - strand at 933.225 kb on + strand at 933.232 kb on + strand at 933.263 kb on + strand at 933.264 kb on - strand at 933.302 kb on + strand, within ABZR87_RS04460 at 933.302 kb on + strand, within ABZR87_RS04460 at 933.302 kb on + strand, within ABZR87_RS04460 at 933.303 kb on - strand, within ABZR87_RS04460 at 933.314 kb on - strand, within ABZR87_RS04460 at 933.314 kb on - strand, within ABZR87_RS04460 at 933.360 kb on + strand, within ABZR87_RS04460 at 933.360 kb on + strand, within ABZR87_RS04460 at 933.360 kb on + strand, within ABZR87_RS04460 at 933.379 kb on + strand, within ABZR87_RS04460 at 933.407 kb on - strand, within ABZR87_RS04460 at 933.450 kb on + strand, within ABZR87_RS04460 at 933.450 kb on + strand, within ABZR87_RS04460 at 933.451 kb on - strand, within ABZR87_RS04460 at 933.464 kb on + strand, within ABZR87_RS04460 at 933.464 kb on + strand, within ABZR87_RS04460 at 933.464 kb on + strand, within ABZR87_RS04460 at 933.464 kb on + strand, within ABZR87_RS04460 at 933.495 kb on + strand, within ABZR87_RS04460 at 933.495 kb on + strand, within ABZR87_RS04460 at 933.496 kb on - strand, within ABZR87_RS04460 at 933.496 kb on - strand, within ABZR87_RS04460 at 933.503 kb on + strand, within ABZR87_RS04460 at 933.503 kb on + strand, within ABZR87_RS04460 at 933.503 kb on + strand, within ABZR87_RS04460 at 933.503 kb on + strand, within ABZR87_RS04460 at 933.504 kb on - strand, within ABZR87_RS04460 at 933.537 kb on + strand at 933.537 kb on + strand at 933.537 kb on + strand at 933.537 kb on + strand at 933.538 kb on - strand at 933.538 kb on - strand at 933.538 kb on - strand at 933.538 kb on - strand at 933.559 kb on + strand at 933.560 kb on - strand at 933.560 kb on - strand at 933.736 kb on + strand, within ABZR87_RS04465 at 933.736 kb on + strand, within ABZR87_RS04465 at 933.737 kb on - strand, within ABZR87_RS04465 at 933.737 kb on - strand, within ABZR87_RS04465 at 933.737 kb on - strand, within ABZR87_RS04465 at 933.737 kb on - strand, within ABZR87_RS04465 at 933.816 kb on + strand, within ABZR87_RS04465 at 933.892 kb on + strand, within ABZR87_RS04465 at 933.893 kb on - strand, within ABZR87_RS04465 at 933.893 kb on - strand, within ABZR87_RS04465 at 933.893 kb on - strand, within ABZR87_RS04465 at 933.893 kb on - strand, within ABZR87_RS04465 at 933.936 kb on + strand at 933.937 kb on - strand at 933.937 kb on - strand at 933.937 kb on - strand at 934.041 kb on + strand, within ABZR87_RS04470 at 934.068 kb on + strand, within ABZR87_RS04470 at 934.069 kb on - strand, within ABZR87_RS04470 at 934.069 kb on - strand, within ABZR87_RS04470 at 934.149 kb on + strand, within ABZR87_RS04470 at 934.149 kb on + strand, within ABZR87_RS04470 at 934.149 kb on + strand, within ABZR87_RS04470 at 934.149 kb on + strand, within ABZR87_RS04470 at 934.149 kb on + strand, within ABZR87_RS04470 at 934.150 kb on - strand, within ABZR87_RS04470 at 934.160 kb on + strand, within ABZR87_RS04470 at 934.255 kb on - strand at 934.273 kb on + strand at 934.274 kb on - strand at 934.396 kb on + strand at 934.396 kb on + strand at 934.397 kb on - strand at 934.397 kb on - strand at 934.413 kb on + strand at 934.413 kb on + strand at 934.414 kb on - strand at 934.414 kb on - strand at 934.414 kb on - strand at 934.437 kb on + strand at 934.438 kb on - strand at 934.438 kb on - strand at 934.438 kb on - strand at 934.481 kb on + strand at 934.482 kb on - strand at 934.577 kb on + strand at 934.599 kb on + strand at 934.599 kb on + strand at 934.599 kb on + strand at 934.632 kb on + strand at 934.632 kb on + strand at 934.633 kb on - strand at 934.633 kb on - strand at 934.633 kb on - strand at 934.634 kb on + strand at 934.634 kb on + strand at 934.634 kb on + strand at 934.634 kb on + strand at 934.634 kb on + strand at 934.634 kb on + strand at 934.634 kb on + strand at 934.635 kb on - strand at 934.635 kb on - strand at 934.645 kb on + strand at 934.645 kb on + strand at 934.645 kb on + strand at 934.646 kb on - strand at 934.655 kb on - strand at 934.676 kb on + strand at 934.676 kb on + strand at 934.676 kb on + strand at 934.677 kb on - strand at 934.677 kb on - strand at 934.677 kb on - strand at 934.677 kb on - strand at 934.750 kb on - strand at 934.772 kb on + strand at 934.773 kb on - strand at 934.832 kb on + strand, within ABZR87_RS04475 at 934.832 kb on + strand, within ABZR87_RS04475 at 934.833 kb on - strand, within ABZR87_RS04475 at 934.836 kb on + strand, within ABZR87_RS04475 at 934.836 kb on + strand, within ABZR87_RS04475 at 934.836 kb on + strand, within ABZR87_RS04475 at 934.836 kb on + strand, within ABZR87_RS04475 at 934.836 kb on + strand, within ABZR87_RS04475 at 934.836 kb on + strand, within ABZR87_RS04475 at 934.836 kb on + strand, within ABZR87_RS04475 at 934.836 kb on + strand, within ABZR87_RS04475 at 934.836 kb on + strand, within ABZR87_RS04475 at 934.837 kb on - strand, within ABZR87_RS04475 at 934.837 kb on - strand, within ABZR87_RS04475 at 934.867 kb on + strand, within ABZR87_RS04475
Per-strain Table
Position Strand Gene LocusTag Fraction root sample 5; outgrowth in LB remove 932,605 + ABZR87_RS04455 0.70 -0.6 932,841 + +0.7 932,883 + -0.7 932,883 + +0.5 932,883 + +2.3 932,884 - -1.0 932,884 - +0.9 932,884 - +0.2 932,906 + -0.9 932,906 + -0.7 932,906 + +0.5 932,939 + +0.2 932,939 + +1.4 932,940 - +0.5 932,940 - +1.0 932,990 + +2.0 932,993 + -0.5 932,993 + -0.5 932,993 + +0.1 932,994 - -0.9 933,225 + -1.7 933,232 + +0.7 933,263 + -1.3 933,264 - +1.4 933,302 + ABZR87_RS04460 0.17 -1.5 933,302 + ABZR87_RS04460 0.17 -1.3 933,302 + ABZR87_RS04460 0.17 +0.4 933,303 - ABZR87_RS04460 0.17 +2.3 933,314 - ABZR87_RS04460 0.21 -0.3 933,314 - ABZR87_RS04460 0.21 -0.7 933,360 + ABZR87_RS04460 0.36 +0.1 933,360 + ABZR87_RS04460 0.36 -0.1 933,360 + ABZR87_RS04460 0.36 +9.8 933,379 + ABZR87_RS04460 0.42 -0.0 933,407 - ABZR87_RS04460 0.51 +1.4 933,450 + ABZR87_RS04460 0.65 +0.2 933,450 + ABZR87_RS04460 0.65 +1.9 933,451 - ABZR87_RS04460 0.66 -0.6 933,464 + ABZR87_RS04460 0.70 -0.5 933,464 + ABZR87_RS04460 0.70 -1.9 933,464 + ABZR87_RS04460 0.70 -0.6 933,464 + ABZR87_RS04460 0.70 +0.3 933,495 + ABZR87_RS04460 0.80 -1.0 933,495 + ABZR87_RS04460 0.80 -0.3 933,496 - ABZR87_RS04460 0.80 -0.3 933,496 - ABZR87_RS04460 0.80 -0.8 933,503 + ABZR87_RS04460 0.83 -1.1 933,503 + ABZR87_RS04460 0.83 +0.2 933,503 + ABZR87_RS04460 0.83 +0.9 933,503 + ABZR87_RS04460 0.83 -0.7 933,504 - ABZR87_RS04460 0.83 +0.0 933,537 + -1.0 933,537 + +1.0 933,537 + +0.6 933,537 + +3.3 933,538 - +0.3 933,538 - +0.1 933,538 - +0.3 933,538 - +0.7 933,559 + +1.4 933,560 - -0.0 933,560 - -0.1 933,736 + ABZR87_RS04465 0.40 +0.3 933,736 + ABZR87_RS04465 0.40 -0.3 933,737 - ABZR87_RS04465 0.40 +0.0 933,737 - ABZR87_RS04465 0.40 -0.6 933,737 - ABZR87_RS04465 0.40 -0.5 933,737 - ABZR87_RS04465 0.40 +0.8 933,816 + ABZR87_RS04465 0.64 -0.7 933,892 + ABZR87_RS04465 0.87 -1.9 933,893 - ABZR87_RS04465 0.88 -0.1 933,893 - ABZR87_RS04465 0.88 +1.4 933,893 - ABZR87_RS04465 0.88 +3.6 933,893 - ABZR87_RS04465 0.88 -1.5 933,936 + +0.3 933,937 - -0.2 933,937 - +0.5 933,937 - +0.1 934,041 + ABZR87_RS04470 0.32 +2.3 934,068 + ABZR87_RS04470 0.41 -0.7 934,069 - ABZR87_RS04470 0.41 +0.7 934,069 - ABZR87_RS04470 0.41 +1.0 934,149 + ABZR87_RS04470 0.67 -0.1 934,149 + ABZR87_RS04470 0.67 -1.2 934,149 + ABZR87_RS04470 0.67 +2.6 934,149 + ABZR87_RS04470 0.67 -0.8 934,149 + ABZR87_RS04470 0.67 -1.7 934,150 - ABZR87_RS04470 0.67 -0.3 934,160 + ABZR87_RS04470 0.70 +2.4 934,255 - +0.2 934,273 + -0.2 934,274 - -0.3 934,396 + -0.7 934,396 + +0.3 934,397 - +2.0 934,397 - -0.4 934,413 + -0.5 934,413 + -1.9 934,414 - +1.4 934,414 - +0.2 934,414 - +0.6 934,437 + -1.0 934,438 - -0.4 934,438 - +0.4 934,438 - -1.4 934,481 + -0.2 934,482 - -1.0 934,577 + +0.4 934,599 + -0.8 934,599 + -0.3 934,599 + -0.3 934,632 + -0.5 934,632 + -0.3 934,633 - +1.7 934,633 - -1.0 934,633 - +0.9 934,634 + +0.4 934,634 + -1.2 934,634 + +1.7 934,634 + -0.8 934,634 + -0.4 934,634 + +0.3 934,634 + +0.6 934,635 - -2.3 934,635 - +0.0 934,645 + +0.6 934,645 + -0.7 934,645 + -0.8 934,646 - -0.5 934,655 - -0.4 934,676 + +0.7 934,676 + -2.1 934,676 + -1.1 934,677 - +3.6 934,677 - -0.6 934,677 - +0.2 934,677 - +0.1 934,750 - +2.9 934,772 + -0.6 934,773 - +0.9 934,832 + ABZR87_RS04475 0.23 +0.5 934,832 + ABZR87_RS04475 0.23 +1.4 934,833 - ABZR87_RS04475 0.23 -1.0 934,836 + ABZR87_RS04475 0.24 -1.8 934,836 + ABZR87_RS04475 0.24 +0.3 934,836 + ABZR87_RS04475 0.24 -0.3 934,836 + ABZR87_RS04475 0.24 -0.6 934,836 + ABZR87_RS04475 0.24 +0.6 934,836 + ABZR87_RS04475 0.24 -0.1 934,836 + ABZR87_RS04475 0.24 -1.3 934,836 + ABZR87_RS04475 0.24 -2.6 934,836 + ABZR87_RS04475 0.24 -2.4 934,837 - ABZR87_RS04475 0.25 -1.7 934,837 - ABZR87_RS04475 0.25 +0.7 934,867 + ABZR87_RS04475 0.33 +0.8
Or see this region's nucleotide sequence