Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5965 and Pf1N1B4_5966 overlap by 8 nucleotides Pf1N1B4_5966 and Pf1N1B4_5967 overlap by 4 nucleotides Pf1N1B4_5967 and Pf1N1B4_5968 are separated by 71 nucleotides
Pf1N1B4_5965 - D-amino-acid oxidase (EC 1.4.3.3), at 413,298 to 414,398
_5965
Pf1N1B4_5966 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 414,391 to 414,627
_5966
Pf1N1B4_5967 - Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster, at 414,624 to 415,880
_5967
Pf1N1B4_5968 - Transcriptional regulator, AraC family, at 415,952 to 416,701
_5968
Position (kb) 414
415
416 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 413.894 kb on - strand, within Pf1N1B4_5965 at 413.958 kb on - strand, within Pf1N1B4_5965 at 413.976 kb on + strand, within Pf1N1B4_5965 at 413.977 kb on - strand at 414.023 kb on + strand, within Pf1N1B4_5965 at 414.138 kb on + strand, within Pf1N1B4_5965 at 414.138 kb on + strand, within Pf1N1B4_5965 at 414.139 kb on - strand, within Pf1N1B4_5965 at 414.391 kb on - strand at 414.918 kb on + strand, within Pf1N1B4_5967 at 414.919 kb on - strand, within Pf1N1B4_5967 at 414.955 kb on + strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967 at 415.558 kb on + strand, within Pf1N1B4_5967 at 415.558 kb on + strand, within Pf1N1B4_5967 at 415.955 kb on + strand at 415.956 kb on - strand at 415.956 kb on - strand at 416.258 kb on + strand, within Pf1N1B4_5968 at 416.259 kb on - strand, within Pf1N1B4_5968 at 416.259 kb on - strand, within Pf1N1B4_5968 at 416.619 kb on - strand, within Pf1N1B4_5968 at 416.830 kb on + strand at 416.831 kb on - strand at 416.861 kb on - strand at 416.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 413,894 - Pf1N1B4_5965 0.54 -0.3 413,958 - Pf1N1B4_5965 0.60 -0.4 413,976 + Pf1N1B4_5965 0.62 -1.2 413,977 - +0.2 414,023 + Pf1N1B4_5965 0.66 -0.4 414,138 + Pf1N1B4_5965 0.76 +0.1 414,138 + Pf1N1B4_5965 0.76 -1.1 414,139 - Pf1N1B4_5965 0.76 -0.8 414,391 - +0.2 414,918 + Pf1N1B4_5967 0.23 -0.5 414,919 - Pf1N1B4_5967 0.23 -0.3 414,955 + Pf1N1B4_5967 0.26 +1.8 414,956 - Pf1N1B4_5967 0.26 -1.7 414,956 - Pf1N1B4_5967 0.26 -1.1 414,956 - Pf1N1B4_5967 0.26 -0.8 415,558 + Pf1N1B4_5967 0.74 -1.8 415,558 + Pf1N1B4_5967 0.74 -2.1 415,955 + -2.0 415,956 - -1.2 415,956 - +0.1 416,258 + Pf1N1B4_5968 0.41 +0.5 416,259 - Pf1N1B4_5968 0.41 -0.8 416,259 - Pf1N1B4_5968 0.41 -2.2 416,619 - Pf1N1B4_5968 0.89 -0.2 416,830 + -3.1 416,831 - -0.2 416,861 - +1.4 416,861 - +0.1
Or see this region's nucleotide sequence