Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5628 and Pf1N1B4_5629 are separated by 14 nucleotides Pf1N1B4_5629 and Pf1N1B4_5630 are separated by 20 nucleotides Pf1N1B4_5630 and Pf1N1B4_5631 are separated by 15 nucleotides
Pf1N1B4_5628 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 55,321 to 56,343
_5628
Pf1N1B4_5629 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 56,358 to 57,932
_5629
Pf1N1B4_5630 - hypothetical protein, at 57,953 to 58,084
_5630
Pf1N1B4_5631 - Sigma-54 dependent transcriptional regulator, at 58,100 to 59,515
_5631
Position (kb)
56
57
58 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 55.400 kb on + strand at 55.486 kb on + strand, within Pf1N1B4_5628 at 55.487 kb on - strand, within Pf1N1B4_5628 at 55.564 kb on + strand, within Pf1N1B4_5628 at 55.584 kb on + strand, within Pf1N1B4_5628 at 55.643 kb on - strand, within Pf1N1B4_5628 at 55.892 kb on - strand, within Pf1N1B4_5628 at 56.023 kb on + strand, within Pf1N1B4_5628 at 56.024 kb on - strand, within Pf1N1B4_5628 at 56.024 kb on - strand, within Pf1N1B4_5628 at 56.024 kb on - strand, within Pf1N1B4_5628 at 56.765 kb on - strand, within Pf1N1B4_5629 at 57.096 kb on + strand, within Pf1N1B4_5629 at 57.096 kb on + strand, within Pf1N1B4_5629 at 57.097 kb on - strand, within Pf1N1B4_5629 at 57.098 kb on + strand, within Pf1N1B4_5629 at 57.099 kb on - strand, within Pf1N1B4_5629 at 57.099 kb on - strand, within Pf1N1B4_5629 at 57.099 kb on - strand, within Pf1N1B4_5629 at 57.106 kb on + strand, within Pf1N1B4_5629 at 57.107 kb on - strand, within Pf1N1B4_5629 at 57.110 kb on - strand, within Pf1N1B4_5629 at 57.229 kb on - strand, within Pf1N1B4_5629 at 57.512 kb on + strand, within Pf1N1B4_5629 at 57.769 kb on - strand, within Pf1N1B4_5629 at 57.894 kb on + strand at 58.002 kb on - strand, within Pf1N1B4_5630 at 58.078 kb on + strand at 58.078 kb on + strand at 58.079 kb on - strand at 58.082 kb on + strand at 58.082 kb on + strand at 58.083 kb on - strand at 58.083 kb on - strand at 58.083 kb on - strand at 58.142 kb on - strand at 58.557 kb on - strand, within Pf1N1B4_5631 at 58.557 kb on - strand, within Pf1N1B4_5631 at 58.557 kb on - strand, within Pf1N1B4_5631 at 58.732 kb on - strand, within Pf1N1B4_5631 at 58.732 kb on - strand, within Pf1N1B4_5631 at 58.732 kb on - strand, within Pf1N1B4_5631
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 55,400 + +0.7 55,486 + Pf1N1B4_5628 0.16 -1.3 55,487 - Pf1N1B4_5628 0.16 -1.1 55,564 + Pf1N1B4_5628 0.24 -0.8 55,584 + Pf1N1B4_5628 0.26 +1.1 55,643 - Pf1N1B4_5628 0.31 -2.6 55,892 - Pf1N1B4_5628 0.56 -1.6 56,023 + Pf1N1B4_5628 0.69 +0.0 56,024 - Pf1N1B4_5628 0.69 +0.7 56,024 - Pf1N1B4_5628 0.69 -0.9 56,024 - Pf1N1B4_5628 0.69 +0.2 56,765 - Pf1N1B4_5629 0.26 -1.2 57,096 + Pf1N1B4_5629 0.47 -0.8 57,096 + Pf1N1B4_5629 0.47 -2.3 57,097 - Pf1N1B4_5629 0.47 +1.1 57,098 + Pf1N1B4_5629 0.47 -3.1 57,099 - Pf1N1B4_5629 0.47 +0.2 57,099 - Pf1N1B4_5629 0.47 +2.3 57,099 - Pf1N1B4_5629 0.47 -1.1 57,106 + Pf1N1B4_5629 0.47 -1.1 57,107 - Pf1N1B4_5629 0.48 -2.7 57,110 - Pf1N1B4_5629 0.48 -0.7 57,229 - Pf1N1B4_5629 0.55 -1.4 57,512 + Pf1N1B4_5629 0.73 -1.8 57,769 - Pf1N1B4_5629 0.90 -0.5 57,894 + -0.1 58,002 - Pf1N1B4_5630 0.37 +1.0 58,078 + -1.1 58,078 + -1.8 58,079 - -0.4 58,082 + -0.6 58,082 + -0.3 58,083 - +0.7 58,083 - +0.3 58,083 - -0.8 58,142 - +0.4 58,557 - Pf1N1B4_5631 0.32 +1.0 58,557 - Pf1N1B4_5631 0.32 -0.4 58,557 - Pf1N1B4_5631 0.32 -0.6 58,732 - Pf1N1B4_5631 0.45 +1.1 58,732 - Pf1N1B4_5631 0.45 -0.8 58,732 - Pf1N1B4_5631 0.45 -1.6
Or see this region's nucleotide sequence