Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5441 and Pf1N1B4_5442 are separated by 164 nucleotides Pf1N1B4_5442 and Pf1N1B4_5443 are separated by 655 nucleotides
Pf1N1B4_5441 - hypothetical protein, at 186,749 to 187,255
_5441
Pf1N1B4_5442 - hypothetical protein, at 187,420 to 187,551
_5442
Pf1N1B4_5443 - hypothetical protein, at 188,207 to 188,659
_5443
Position (kb) 187
188 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 186.436 kb on + strand at 186.437 kb on - strand at 186.461 kb on + strand at 186.461 kb on + strand at 186.461 kb on + strand at 186.461 kb on + strand at 186.462 kb on - strand at 186.462 kb on - strand at 186.462 kb on - strand at 186.466 kb on + strand at 186.466 kb on + strand at 186.466 kb on + strand at 186.466 kb on + strand at 186.466 kb on + strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.545 kb on + strand at 186.545 kb on + strand at 186.550 kb on - strand at 186.559 kb on + strand at 186.560 kb on - strand at 186.560 kb on - strand at 186.705 kb on + strand at 187.081 kb on + strand, within Pf1N1B4_5441 at 187.081 kb on + strand, within Pf1N1B4_5441 at 187.082 kb on - strand, within Pf1N1B4_5441 at 187.082 kb on - strand, within Pf1N1B4_5441 at 187.082 kb on - strand, within Pf1N1B4_5441 at 187.082 kb on - strand, within Pf1N1B4_5441 at 187.084 kb on - strand, within Pf1N1B4_5441 at 187.124 kb on + strand, within Pf1N1B4_5441 at 187.125 kb on - strand, within Pf1N1B4_5441 at 187.125 kb on - strand, within Pf1N1B4_5441 at 187.125 kb on - strand, within Pf1N1B4_5441 at 187.142 kb on + strand, within Pf1N1B4_5441 at 187.142 kb on + strand, within Pf1N1B4_5441 at 187.142 kb on + strand, within Pf1N1B4_5441 at 187.142 kb on + strand, within Pf1N1B4_5441 at 187.143 kb on - strand, within Pf1N1B4_5441 at 187.143 kb on - strand, within Pf1N1B4_5441 at 187.143 kb on - strand, within Pf1N1B4_5441 at 187.143 kb on - strand, within Pf1N1B4_5441 at 187.143 kb on - strand, within Pf1N1B4_5441 at 187.143 kb on - strand, within Pf1N1B4_5441 at 187.143 kb on - strand at 187.146 kb on + strand, within Pf1N1B4_5441 at 187.146 kb on + strand, within Pf1N1B4_5441 at 187.147 kb on - strand, within Pf1N1B4_5441 at 187.147 kb on - strand, within Pf1N1B4_5441 at 187.147 kb on - strand, within Pf1N1B4_5441 at 187.147 kb on - strand, within Pf1N1B4_5441 at 187.147 kb on - strand, within Pf1N1B4_5441 at 187.165 kb on - strand, within Pf1N1B4_5441 at 187.167 kb on + strand, within Pf1N1B4_5441 at 187.167 kb on + strand, within Pf1N1B4_5441 at 187.168 kb on - strand, within Pf1N1B4_5441 at 187.168 kb on - strand, within Pf1N1B4_5441 at 187.168 kb on - strand, within Pf1N1B4_5441 at 187.172 kb on + strand, within Pf1N1B4_5441 at 187.172 kb on + strand, within Pf1N1B4_5441 at 187.172 kb on + strand, within Pf1N1B4_5441 at 187.172 kb on + strand, within Pf1N1B4_5441 at 187.173 kb on - strand, within Pf1N1B4_5441 at 187.173 kb on - strand, within Pf1N1B4_5441 at 187.328 kb on + strand at 187.328 kb on + strand at 187.329 kb on - strand at 187.461 kb on - strand, within Pf1N1B4_5442 at 187.461 kb on - strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.525 kb on - strand, within Pf1N1B4_5442 at 187.525 kb on - strand, within Pf1N1B4_5442 at 187.525 kb on - strand, within Pf1N1B4_5442 at 187.570 kb on - strand at 187.638 kb on + strand at 187.638 kb on + strand at 187.638 kb on + strand at 187.638 kb on + strand at 187.638 kb on + strand at 187.638 kb on + strand at 187.639 kb on - strand at 187.648 kb on + strand at 187.648 kb on + strand at 187.648 kb on + strand at 187.649 kb on - strand at 187.725 kb on + strand at 187.769 kb on + strand at 187.769 kb on + strand at 187.769 kb on + strand at 187.769 kb on + strand at 187.770 kb on - strand at 187.770 kb on - strand at 187.804 kb on + strand at 187.804 kb on + strand at 187.804 kb on + strand at 187.804 kb on + strand at 187.804 kb on + strand at 187.805 kb on - strand at 187.816 kb on + strand at 187.816 kb on + strand at 187.816 kb on + strand at 187.816 kb on + strand at 187.817 kb on - strand at 187.817 kb on - strand at 187.817 kb on - strand at 187.831 kb on + strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.891 kb on + strand at 187.900 kb on + strand at 187.900 kb on + strand at 187.900 kb on + strand at 187.900 kb on + strand at 187.940 kb on - strand at 187.940 kb on - strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.948 kb on - strand at 187.948 kb on - strand at 187.948 kb on - strand at 187.963 kb on + strand at 187.963 kb on + strand at 187.969 kb on - strand at 187.971 kb on + strand at 187.971 kb on + strand at 187.972 kb on - strand at 187.993 kb on + strand at 188.032 kb on + strand at 188.032 kb on + strand at 188.033 kb on - strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.095 kb on - strand at 188.095 kb on - strand at 188.095 kb on - strand at 188.095 kb on - strand at 188.095 kb on - strand at 188.199 kb on - strand at 188.202 kb on + strand at 188.203 kb on - strand at 188.203 kb on - strand at 188.203 kb on - strand at 188.203 kb on - strand at 188.205 kb on + strand at 188.206 kb on - strand at 188.206 kb on - strand at 188.209 kb on - strand at 188.209 kb on - strand at 188.238 kb on + strand at 188.239 kb on - strand at 188.239 kb on - strand at 188.239 kb on - strand at 188.239 kb on - strand at 188.239 kb on - strand at 188.254 kb on - strand, within Pf1N1B4_5443 at 188.254 kb on - strand, within Pf1N1B4_5443 at 188.288 kb on - strand, within Pf1N1B4_5443 at 188.323 kb on + strand, within Pf1N1B4_5443 at 188.323 kb on + strand, within Pf1N1B4_5443 at 188.427 kb on + strand, within Pf1N1B4_5443 at 188.427 kb on + strand, within Pf1N1B4_5443 at 188.427 kb on + strand, within Pf1N1B4_5443 at 188.428 kb on - strand, within Pf1N1B4_5443 at 188.428 kb on - strand, within Pf1N1B4_5443 at 188.428 kb on - strand, within Pf1N1B4_5443 at 188.529 kb on - strand, within Pf1N1B4_5443 at 188.529 kb on - strand, within Pf1N1B4_5443 at 188.542 kb on - strand, within Pf1N1B4_5443 at 188.542 kb on - strand, within Pf1N1B4_5443
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 186,436 + +2.7 186,437 - +1.1 186,461 + +0.1 186,461 + +1.6 186,461 + -1.7 186,461 + +1.1 186,462 - -1.9 186,462 - -1.5 186,462 - -0.9 186,466 + +1.1 186,466 + -0.7 186,466 + +0.7 186,466 + -2.7 186,466 + -2.2 186,467 - -1.7 186,467 - -1.2 186,545 + -1.7 186,545 + -2.8 186,550 - -0.3 186,559 + -0.2 186,560 - +1.4 186,560 - -0.6 186,705 + -1.0 187,081 + Pf1N1B4_5441 0.65 +1.4 187,081 + Pf1N1B4_5441 0.65 -2.0 187,082 - Pf1N1B4_5441 0.66 +0.3 187,082 - Pf1N1B4_5441 0.66 -0.5 187,082 - Pf1N1B4_5441 0.66 +0.1 187,082 - Pf1N1B4_5441 0.66 +0.9 187,084 - Pf1N1B4_5441 0.66 +0.1 187,124 + Pf1N1B4_5441 0.74 +1.8 187,125 - Pf1N1B4_5441 0.74 -1.1 187,125 - Pf1N1B4_5441 0.74 -0.5 187,125 - Pf1N1B4_5441 0.74 -2.2 187,142 + Pf1N1B4_5441 0.78 +0.8 187,142 + Pf1N1B4_5441 0.78 -0.1 187,142 + Pf1N1B4_5441 0.78 -1.2 187,142 + Pf1N1B4_5441 0.78 -0.9 187,143 - Pf1N1B4_5441 0.78 -0.5 187,143 - Pf1N1B4_5441 0.78 -2.5 187,143 - Pf1N1B4_5441 0.78 +0.1 187,143 - Pf1N1B4_5441 0.78 -0.1 187,143 - Pf1N1B4_5441 0.78 +2.7 187,143 - Pf1N1B4_5441 0.78 -1.2 187,143 - +0.1 187,146 + Pf1N1B4_5441 0.78 +2.3 187,146 + Pf1N1B4_5441 0.78 -0.7 187,147 - Pf1N1B4_5441 0.79 -1.6 187,147 - Pf1N1B4_5441 0.79 +0.4 187,147 - Pf1N1B4_5441 0.79 -0.2 187,147 - Pf1N1B4_5441 0.79 -1.5 187,147 - Pf1N1B4_5441 0.79 -1.7 187,165 - Pf1N1B4_5441 0.82 -0.3 187,167 + Pf1N1B4_5441 0.82 -0.6 187,167 + Pf1N1B4_5441 0.82 +0.6 187,168 - Pf1N1B4_5441 0.83 +0.4 187,168 - Pf1N1B4_5441 0.83 -0.5 187,168 - Pf1N1B4_5441 0.83 +0.5 187,172 + Pf1N1B4_5441 0.83 +0.5 187,172 + Pf1N1B4_5441 0.83 -1.1 187,172 + Pf1N1B4_5441 0.83 -1.9 187,172 + Pf1N1B4_5441 0.83 +0.3 187,173 - Pf1N1B4_5441 0.84 -0.5 187,173 - Pf1N1B4_5441 0.84 +0.3 187,328 + -0.7 187,328 + +0.1 187,329 - +1.4 187,461 - Pf1N1B4_5442 0.31 -1.3 187,461 - Pf1N1B4_5442 0.31 -1.9 187,524 + Pf1N1B4_5442 0.79 -0.9 187,524 + Pf1N1B4_5442 0.79 -1.7 187,524 + Pf1N1B4_5442 0.79 -1.1 187,524 + Pf1N1B4_5442 0.79 -1.0 187,524 + Pf1N1B4_5442 0.79 -0.7 187,524 + Pf1N1B4_5442 0.79 -0.9 187,525 - Pf1N1B4_5442 0.80 -1.9 187,525 - Pf1N1B4_5442 0.80 -0.6 187,525 - Pf1N1B4_5442 0.80 -0.5 187,570 - -0.2 187,638 + -1.2 187,638 + -0.5 187,638 + +0.8 187,638 + +0.5 187,638 + -1.3 187,638 + -1.3 187,639 - +0.8 187,648 + +0.7 187,648 + +0.7 187,648 + +1.4 187,649 - -2.3 187,725 + -0.9 187,769 + +0.4 187,769 + +0.7 187,769 + -1.9 187,769 + +0.1 187,770 - -1.0 187,770 - -2.7 187,804 + -0.5 187,804 + -0.9 187,804 + +1.6 187,804 + +0.7 187,804 + +1.1 187,805 - -0.5 187,816 + +0.2 187,816 + -0.1 187,816 + +0.4 187,816 + -1.0 187,817 - -1.2 187,817 - +0.4 187,817 - +0.3 187,831 + -2.8 187,832 - +1.1 187,832 - +2.5 187,832 - -0.4 187,891 + -1.6 187,900 + -1.7 187,900 + -0.5 187,900 + -0.9 187,900 + +1.5 187,940 - +0.9 187,940 - +1.5 187,947 + +0.1 187,947 + -0.2 187,947 + +1.0 187,947 + +1.5 187,947 + -0.1 187,947 + -2.2 187,947 + +0.3 187,947 + +0.5 187,947 + +0.8 187,947 + +1.3 187,947 + +0.0 187,948 - +0.3 187,948 - -2.3 187,948 - +0.1 187,963 + -1.4 187,963 + -1.2 187,969 - +3.4 187,971 + -0.4 187,971 + -1.1 187,972 - -2.2 187,993 + -1.5 188,032 + -0.2 188,032 + +0.9 188,033 - +0.2 188,094 + -0.5 188,094 + -0.2 188,094 + +0.1 188,094 + +2.1 188,094 + -0.2 188,094 + +0.4 188,094 + -1.0 188,094 + +0.1 188,095 - +0.1 188,095 - +0.3 188,095 - -1.7 188,095 - +2.8 188,095 - -0.2 188,199 - -2.3 188,202 + -1.5 188,203 - -0.1 188,203 - -0.8 188,203 - -0.2 188,203 - -2.3 188,205 + +0.4 188,206 - +0.9 188,206 - +1.9 188,209 - +0.6 188,209 - -2.2 188,238 + +0.8 188,239 - -2.1 188,239 - -1.1 188,239 - -0.6 188,239 - +0.5 188,239 - -0.0 188,254 - Pf1N1B4_5443 0.10 -0.6 188,254 - Pf1N1B4_5443 0.10 +0.1 188,288 - Pf1N1B4_5443 0.18 -0.9 188,323 + Pf1N1B4_5443 0.26 +1.8 188,323 + Pf1N1B4_5443 0.26 +1.5 188,427 + Pf1N1B4_5443 0.49 +1.1 188,427 + Pf1N1B4_5443 0.49 -2.0 188,427 + Pf1N1B4_5443 0.49 +0.7 188,428 - Pf1N1B4_5443 0.49 +1.1 188,428 - Pf1N1B4_5443 0.49 -2.2 188,428 - Pf1N1B4_5443 0.49 +1.2 188,529 - Pf1N1B4_5443 0.71 -1.9 188,529 - Pf1N1B4_5443 0.71 -1.5 188,542 - Pf1N1B4_5443 0.74 -0.4 188,542 - Pf1N1B4_5443 0.74 +0.2
Or see this region's nucleotide sequence