Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4698 and Pf1N1B4_4699 are separated by 84 nucleotides Pf1N1B4_4699 and Pf1N1B4_4700 are separated by 591 nucleotides Pf1N1B4_4700 and Pf1N1B4_4701 are separated by 19 nucleotides
Pf1N1B4_4698 - rfbN protein, at 5,191,432 to 5,192,499
_4698
Pf1N1B4_4699 - Multidrug translocase MdfA, at 5,192,584 to 5,193,825
_4699
Pf1N1B4_4700 - FIG00956898: hypothetical protein, at 5,194,417 to 5,194,605
_4700
Pf1N1B4_4701 - Fe2+/Zn2+ uptake regulation proteins, at 5,194,625 to 5,194,996
_4701
Position (kb) 5192
5193
5194 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5191.691 kb on + strand, within Pf1N1B4_4698 at 5191.692 kb on - strand, within Pf1N1B4_4698 at 5191.728 kb on + strand, within Pf1N1B4_4698 at 5191.730 kb on + strand, within Pf1N1B4_4698 at 5191.731 kb on - strand at 5191.731 kb on - strand, within Pf1N1B4_4698 at 5191.731 kb on - strand, within Pf1N1B4_4698 at 5191.811 kb on + strand, within Pf1N1B4_4698 at 5192.082 kb on - strand, within Pf1N1B4_4698 at 5192.082 kb on - strand, within Pf1N1B4_4698 at 5192.105 kb on + strand, within Pf1N1B4_4698 at 5192.105 kb on + strand, within Pf1N1B4_4698 at 5192.106 kb on - strand, within Pf1N1B4_4698 at 5192.106 kb on - strand, within Pf1N1B4_4698 at 5192.157 kb on - strand, within Pf1N1B4_4698 at 5192.552 kb on - strand at 5192.573 kb on - strand at 5192.776 kb on - strand, within Pf1N1B4_4699 at 5192.844 kb on - strand, within Pf1N1B4_4699 at 5193.261 kb on - strand, within Pf1N1B4_4699 at 5193.651 kb on - strand, within Pf1N1B4_4699 at 5193.743 kb on + strand at 5193.744 kb on - strand at 5193.802 kb on + strand at 5193.803 kb on - strand at 5193.803 kb on - strand at 5193.804 kb on + strand at 5193.805 kb on - strand at 5193.809 kb on - strand at 5194.049 kb on + strand at 5194.050 kb on - strand at 5194.094 kb on + strand at 5194.116 kb on + strand at 5194.534 kb on + strand, within Pf1N1B4_4700 at 5194.534 kb on + strand, within Pf1N1B4_4700
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 5,191,691 + Pf1N1B4_4698 0.24 -0.9 5,191,692 - Pf1N1B4_4698 0.24 -0.9 5,191,728 + Pf1N1B4_4698 0.28 +0.1 5,191,730 + Pf1N1B4_4698 0.28 -1.9 5,191,731 - -0.3 5,191,731 - Pf1N1B4_4698 0.28 -1.3 5,191,731 - Pf1N1B4_4698 0.28 -2.5 5,191,811 + Pf1N1B4_4698 0.35 -1.6 5,192,082 - Pf1N1B4_4698 0.61 +0.5 5,192,082 - Pf1N1B4_4698 0.61 -0.9 5,192,105 + Pf1N1B4_4698 0.63 +0.7 5,192,105 + Pf1N1B4_4698 0.63 -0.2 5,192,106 - Pf1N1B4_4698 0.63 -0.8 5,192,106 - Pf1N1B4_4698 0.63 +1.3 5,192,157 - Pf1N1B4_4698 0.68 -1.6 5,192,552 - +1.4 5,192,573 - -2.1 5,192,776 - Pf1N1B4_4699 0.15 -0.4 5,192,844 - Pf1N1B4_4699 0.21 -1.6 5,193,261 - Pf1N1B4_4699 0.55 -1.3 5,193,651 - Pf1N1B4_4699 0.86 -2.5 5,193,743 + -1.3 5,193,744 - -1.2 5,193,802 + -0.6 5,193,803 - -2.3 5,193,803 - +0.7 5,193,804 + +1.3 5,193,805 - -0.0 5,193,809 - +2.3 5,194,049 + -3.0 5,194,050 - +0.2 5,194,094 + +0.7 5,194,116 + -0.7 5,194,534 + Pf1N1B4_4700 0.62 -3.0 5,194,534 + Pf1N1B4_4700 0.62 +0.3
Or see this region's nucleotide sequence