Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4085

Experiment: Benzethonium chloride 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4083 and Pf1N1B4_4084 are separated by 96 nucleotidesPf1N1B4_4084 and Pf1N1B4_4085 are separated by 168 nucleotidesPf1N1B4_4085 and Pf1N1B4_4086 are separated by 135 nucleotides Pf1N1B4_4083 - Isochorismatase (EC 3.3.2.1), at 4,532,355 to 4,532,888 _4083 Pf1N1B4_4084 - hypothetical protein, at 4,532,985 to 4,533,275 _4084 Pf1N1B4_4085 - probable exported protein YPO0702, at 4,533,444 to 4,534,475 _4085 Pf1N1B4_4086 - transcriptional regulator, LuxR family, at 4,534,611 to 4,535,633 _4086 Position (kb) 4533 4534 4535Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4532.529 kb on + strand, within Pf1N1B4_4083at 4532.529 kb on + strand, within Pf1N1B4_4083at 4532.530 kb on - strand, within Pf1N1B4_4083at 4532.616 kb on + strand, within Pf1N1B4_4083at 4532.616 kb on + strand, within Pf1N1B4_4083at 4532.616 kb on + strand, within Pf1N1B4_4083at 4532.691 kb on + strand, within Pf1N1B4_4083at 4532.692 kb on - strand, within Pf1N1B4_4083at 4532.947 kb on + strandat 4532.948 kb on - strandat 4533.080 kb on - strand, within Pf1N1B4_4084at 4533.239 kb on - strand, within Pf1N1B4_4084at 4533.381 kb on - strandat 4533.413 kb on + strandat 4533.413 kb on + strandat 4533.414 kb on - strandat 4533.414 kb on - strandat 4533.467 kb on + strandat 4533.468 kb on - strandat 4533.527 kb on + strandat 4533.661 kb on + strand, within Pf1N1B4_4085at 4533.662 kb on - strand, within Pf1N1B4_4085at 4533.662 kb on - strand, within Pf1N1B4_4085at 4533.771 kb on - strand, within Pf1N1B4_4085at 4533.866 kb on - strand, within Pf1N1B4_4085at 4533.948 kb on + strand, within Pf1N1B4_4085at 4533.948 kb on + strand, within Pf1N1B4_4085at 4533.949 kb on - strand, within Pf1N1B4_4085at 4533.949 kb on - strand, within Pf1N1B4_4085at 4534.053 kb on + strand, within Pf1N1B4_4085at 4534.054 kb on - strand, within Pf1N1B4_4085at 4534.069 kb on + strand, within Pf1N1B4_4085at 4534.069 kb on + strand, within Pf1N1B4_4085at 4534.069 kb on + strand, within Pf1N1B4_4085at 4534.070 kb on - strand, within Pf1N1B4_4085at 4534.070 kb on - strand, within Pf1N1B4_4085at 4534.070 kb on - strand, within Pf1N1B4_4085at 4534.174 kb on - strand, within Pf1N1B4_4085at 4534.277 kb on + strand, within Pf1N1B4_4085at 4534.371 kb on + strandat 4534.371 kb on + strand, within Pf1N1B4_4085at 4534.371 kb on + strand, within Pf1N1B4_4085at 4534.371 kb on + strand, within Pf1N1B4_4085at 4534.372 kb on - strandat 4534.372 kb on - strandat 4534.451 kb on + strandat 4534.451 kb on + strandat 4534.678 kb on + strandat 4534.998 kb on + strand, within Pf1N1B4_4086at 4534.999 kb on - strand, within Pf1N1B4_4086at 4534.999 kb on - strand, within Pf1N1B4_4086at 4534.999 kb on - strand, within Pf1N1B4_4086at 4535.001 kb on + strand, within Pf1N1B4_4086at 4535.001 kb on + strand, within Pf1N1B4_4086at 4535.026 kb on + strand, within Pf1N1B4_4086at 4535.232 kb on + strand, within Pf1N1B4_4086at 4535.248 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM
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4,532,529 + Pf1N1B4_4083 0.33 -0.4
4,532,529 + Pf1N1B4_4083 0.33 +1.4
4,532,530 - Pf1N1B4_4083 0.33 -0.3
4,532,616 + Pf1N1B4_4083 0.49 +0.6
4,532,616 + Pf1N1B4_4083 0.49 -0.2
4,532,616 + Pf1N1B4_4083 0.49 +0.8
4,532,691 + Pf1N1B4_4083 0.63 -0.4
4,532,692 - Pf1N1B4_4083 0.63 +0.1
4,532,947 + +0.4
4,532,948 - -0.2
4,533,080 - Pf1N1B4_4084 0.33 +0.6
4,533,239 - Pf1N1B4_4084 0.87 +0.1
4,533,381 - +1.4
4,533,413 + +0.3
4,533,413 + -1.0
4,533,414 - -0.7
4,533,414 - -0.7
4,533,467 + +0.3
4,533,468 - -1.7
4,533,527 + -0.7
4,533,661 + Pf1N1B4_4085 0.21 -0.6
4,533,662 - Pf1N1B4_4085 0.21 -0.6
4,533,662 - Pf1N1B4_4085 0.21 +0.2
4,533,771 - Pf1N1B4_4085 0.32 +1.8
4,533,866 - Pf1N1B4_4085 0.41 +0.8
4,533,948 + Pf1N1B4_4085 0.49 +0.6
4,533,948 + Pf1N1B4_4085 0.49 -1.5
4,533,949 - Pf1N1B4_4085 0.49 +0.3
4,533,949 - Pf1N1B4_4085 0.49 -0.3
4,534,053 + Pf1N1B4_4085 0.59 +0.8
4,534,054 - Pf1N1B4_4085 0.59 -0.2
4,534,069 + Pf1N1B4_4085 0.61 -0.6
4,534,069 + Pf1N1B4_4085 0.61 +2.8
4,534,069 + Pf1N1B4_4085 0.61 +0.8
4,534,070 - Pf1N1B4_4085 0.61 +1.3
4,534,070 - Pf1N1B4_4085 0.61 +2.1
4,534,070 - Pf1N1B4_4085 0.61 -1.5
4,534,174 - Pf1N1B4_4085 0.71 +1.8
4,534,277 + Pf1N1B4_4085 0.81 -1.5
4,534,371 + -1.5
4,534,371 + Pf1N1B4_4085 0.90 +0.2
4,534,371 + Pf1N1B4_4085 0.90 +0.3
4,534,371 + Pf1N1B4_4085 0.90 +0.6
4,534,372 - -0.7
4,534,372 - +0.3
4,534,451 + -0.2
4,534,451 + +0.8
4,534,678 + +0.6
4,534,998 + Pf1N1B4_4086 0.38 -0.0
4,534,999 - Pf1N1B4_4086 0.38 -2.2
4,534,999 - Pf1N1B4_4086 0.38 -1.7
4,534,999 - Pf1N1B4_4086 0.38 -1.2
4,535,001 + Pf1N1B4_4086 0.38 +0.6
4,535,001 + Pf1N1B4_4086 0.38 +0.8
4,535,026 + Pf1N1B4_4086 0.41 +0.1
4,535,232 + Pf1N1B4_4086 0.61 -1.2
4,535,248 + +1.8

Or see this region's nucleotide sequence