Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3359 and Pf1N1B4_3360 are separated by 13 nucleotides Pf1N1B4_3360 and Pf1N1B4_3361 are separated by 125 nucleotides Pf1N1B4_3361 and Pf1N1B4_3362 are separated by 13 nucleotides
Pf1N1B4_3359 - Glutamine amidotransferase, class-II, at 3,745,118 to 3,745,894
_3359
Pf1N1B4_3360 - FIG00953701: hypothetical protein, at 3,745,908 to 3,746,450
_3360
Pf1N1B4_3361 - Outer membrane receptor proteins, likely involved in siderophore uptake @ TonB-dependent siderophore receptor, at 3,746,576 to 3,748,687
_3361
Pf1N1B4_3362 - Multidrug resistance protein B, at 3,748,701 to 3,749,912
_3362
Position (kb)
3746
3747
3748
3749 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3745.920 kb on + strand at 3745.920 kb on + strand at 3745.921 kb on - strand at 3745.921 kb on - strand at 3745.921 kb on - strand at 3745.990 kb on - strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.079 kb on - strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.212 kb on - strand, within Pf1N1B4_3360 at 3746.277 kb on + strand, within Pf1N1B4_3360 at 3746.277 kb on + strand, within Pf1N1B4_3360 at 3746.448 kb on + strand at 3746.501 kb on + strand at 3746.534 kb on + strand at 3746.534 kb on + strand at 3746.534 kb on + strand at 3746.535 kb on - strand at 3746.535 kb on - strand at 3746.578 kb on - strand at 3746.668 kb on - strand at 3746.689 kb on - strand at 3746.689 kb on - strand at 3746.689 kb on - strand at 3746.689 kb on - strand at 3746.749 kb on + strand at 3746.749 kb on + strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.752 kb on - strand at 3746.796 kb on + strand, within Pf1N1B4_3361 at 3746.832 kb on + strand, within Pf1N1B4_3361 at 3746.833 kb on - strand, within Pf1N1B4_3361 at 3746.847 kb on + strand, within Pf1N1B4_3361 at 3746.943 kb on + strand, within Pf1N1B4_3361 at 3746.943 kb on + strand, within Pf1N1B4_3361 at 3746.943 kb on + strand, within Pf1N1B4_3361 at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3746.944 kb on - strand at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3746.944 kb on - strand at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3747.055 kb on - strand, within Pf1N1B4_3361 at 3747.055 kb on - strand, within Pf1N1B4_3361 at 3747.055 kb on - strand, within Pf1N1B4_3361 at 3747.084 kb on + strand, within Pf1N1B4_3361 at 3747.084 kb on + strand, within Pf1N1B4_3361 at 3747.244 kb on - strand, within Pf1N1B4_3361 at 3747.244 kb on - strand, within Pf1N1B4_3361 at 3747.297 kb on + strand, within Pf1N1B4_3361 at 3747.297 kb on + strand, within Pf1N1B4_3361 at 3747.298 kb on - strand, within Pf1N1B4_3361 at 3747.312 kb on + strand, within Pf1N1B4_3361 at 3747.313 kb on - strand, within Pf1N1B4_3361 at 3747.370 kb on - strand, within Pf1N1B4_3361 at 3747.504 kb on + strand, within Pf1N1B4_3361 at 3747.504 kb on + strand, within Pf1N1B4_3361 at 3747.525 kb on + strand, within Pf1N1B4_3361 at 3747.525 kb on + strand, within Pf1N1B4_3361 at 3747.525 kb on + strand, within Pf1N1B4_3361 at 3747.526 kb on - strand, within Pf1N1B4_3361 at 3747.582 kb on + strand at 3747.582 kb on + strand, within Pf1N1B4_3361 at 3747.583 kb on - strand, within Pf1N1B4_3361 at 3747.747 kb on + strand, within Pf1N1B4_3361 at 3747.748 kb on - strand, within Pf1N1B4_3361 at 3747.748 kb on - strand, within Pf1N1B4_3361 at 3747.774 kb on + strand, within Pf1N1B4_3361 at 3747.774 kb on + strand, within Pf1N1B4_3361 at 3747.775 kb on - strand, within Pf1N1B4_3361 at 3747.775 kb on - strand, within Pf1N1B4_3361 at 3747.792 kb on + strand, within Pf1N1B4_3361 at 3747.792 kb on + strand at 3747.793 kb on - strand, within Pf1N1B4_3361 at 3747.820 kb on + strand, within Pf1N1B4_3361 at 3747.822 kb on + strand, within Pf1N1B4_3361 at 3747.822 kb on + strand, within Pf1N1B4_3361 at 3747.915 kb on + strand, within Pf1N1B4_3361 at 3747.916 kb on - strand, within Pf1N1B4_3361 at 3747.916 kb on - strand, within Pf1N1B4_3361 at 3747.916 kb on - strand, within Pf1N1B4_3361 at 3747.916 kb on - strand, within Pf1N1B4_3361 at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.984 kb on + strand at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.985 kb on - strand, within Pf1N1B4_3361 at 3748.050 kb on + strand, within Pf1N1B4_3361 at 3748.086 kb on + strand, within Pf1N1B4_3361 at 3748.087 kb on - strand, within Pf1N1B4_3361 at 3748.087 kb on - strand, within Pf1N1B4_3361 at 3748.087 kb on - strand, within Pf1N1B4_3361 at 3748.191 kb on + strand, within Pf1N1B4_3361 at 3748.192 kb on - strand at 3748.192 kb on - strand, within Pf1N1B4_3361 at 3748.192 kb on - strand, within Pf1N1B4_3361 at 3748.192 kb on - strand, within Pf1N1B4_3361 at 3748.192 kb on - strand at 3748.192 kb on - strand at 3748.192 kb on - strand, within Pf1N1B4_3361 at 3748.213 kb on + strand, within Pf1N1B4_3361 at 3748.214 kb on - strand, within Pf1N1B4_3361 at 3748.260 kb on + strand, within Pf1N1B4_3361 at 3748.260 kb on + strand, within Pf1N1B4_3361 at 3748.260 kb on + strand, within Pf1N1B4_3361 at 3748.332 kb on + strand, within Pf1N1B4_3361 at 3748.332 kb on + strand, within Pf1N1B4_3361 at 3748.333 kb on - strand, within Pf1N1B4_3361 at 3748.358 kb on + strand, within Pf1N1B4_3361 at 3748.702 kb on + strand at 3748.702 kb on + strand at 3748.703 kb on - strand at 3748.711 kb on + strand at 3748.711 kb on + strand at 3748.712 kb on - strand at 3748.712 kb on - strand at 3748.792 kb on + strand at 3748.792 kb on + strand at 3748.792 kb on + strand at 3748.792 kb on + strand at 3748.793 kb on - strand at 3748.793 kb on - strand at 3748.793 kb on - strand at 3749.218 kb on + strand at 3749.219 kb on - strand, within Pf1N1B4_3362 at 3749.219 kb on - strand, within Pf1N1B4_3362 at 3749.343 kb on - strand, within Pf1N1B4_3362 at 3749.545 kb on + strand, within Pf1N1B4_3362 at 3749.546 kb on - strand, within Pf1N1B4_3362 at 3749.546 kb on - strand, within Pf1N1B4_3362 at 3749.685 kb on - strand, within Pf1N1B4_3362
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 3,745,920 + -0.3 3,745,920 + -1.0 3,745,921 - +0.5 3,745,921 - -0.9 3,745,921 - -0.3 3,745,990 - Pf1N1B4_3360 0.15 -0.9 3,746,078 + Pf1N1B4_3360 0.31 +0.6 3,746,078 + Pf1N1B4_3360 0.31 -1.0 3,746,078 + Pf1N1B4_3360 0.31 -0.9 3,746,078 + Pf1N1B4_3360 0.31 -0.1 3,746,079 - Pf1N1B4_3360 0.31 -0.9 3,746,211 + Pf1N1B4_3360 0.56 +2.9 3,746,211 + Pf1N1B4_3360 0.56 -1.2 3,746,211 + Pf1N1B4_3360 0.56 +0.1 3,746,211 + Pf1N1B4_3360 0.56 -0.3 3,746,211 + Pf1N1B4_3360 0.56 -1.5 3,746,212 - Pf1N1B4_3360 0.56 +0.1 3,746,277 + Pf1N1B4_3360 0.68 -0.5 3,746,277 + Pf1N1B4_3360 0.68 +0.2 3,746,448 + +0.4 3,746,501 + +1.1 3,746,534 + +1.1 3,746,534 + +0.8 3,746,534 + -1.5 3,746,535 - +0.1 3,746,535 - +1.1 3,746,578 - +0.0 3,746,668 - +1.1 3,746,689 - +0.6 3,746,689 - +1.9 3,746,689 - -1.9 3,746,689 - -1.2 3,746,749 + -3.7 3,746,749 + -0.9 3,746,750 - -1.7 3,746,750 - -0.9 3,746,750 - -0.2 3,746,750 - -2.3 3,746,750 - -0.9 3,746,752 - -0.5 3,746,796 + Pf1N1B4_3361 0.10 -0.0 3,746,832 + Pf1N1B4_3361 0.12 -0.4 3,746,833 - Pf1N1B4_3361 0.12 -0.9 3,746,847 + Pf1N1B4_3361 0.13 -1.7 3,746,943 + Pf1N1B4_3361 0.17 +0.1 3,746,943 + Pf1N1B4_3361 0.17 -3.4 3,746,943 + Pf1N1B4_3361 0.17 -1.2 3,746,944 - Pf1N1B4_3361 0.17 +0.1 3,746,944 - +1.1 3,746,944 - Pf1N1B4_3361 0.17 +0.8 3,746,944 - Pf1N1B4_3361 0.17 -0.7 3,746,944 - +0.1 3,746,944 - Pf1N1B4_3361 0.17 +0.1 3,747,055 - Pf1N1B4_3361 0.23 -2.7 3,747,055 - Pf1N1B4_3361 0.23 -1.2 3,747,055 - Pf1N1B4_3361 0.23 -1.1 3,747,084 + Pf1N1B4_3361 0.24 +0.4 3,747,084 + Pf1N1B4_3361 0.24 -0.6 3,747,244 - Pf1N1B4_3361 0.32 +1.1 3,747,244 - Pf1N1B4_3361 0.32 -1.7 3,747,297 + Pf1N1B4_3361 0.34 -1.7 3,747,297 + Pf1N1B4_3361 0.34 -2.1 3,747,298 - Pf1N1B4_3361 0.34 -0.5 3,747,312 + Pf1N1B4_3361 0.35 +0.4 3,747,313 - Pf1N1B4_3361 0.35 -2.1 3,747,370 - Pf1N1B4_3361 0.38 -2.5 3,747,504 + Pf1N1B4_3361 0.44 -1.5 3,747,504 + Pf1N1B4_3361 0.44 -1.7 3,747,525 + Pf1N1B4_3361 0.45 +0.1 3,747,525 + Pf1N1B4_3361 0.45 -1.5 3,747,525 + Pf1N1B4_3361 0.45 -0.9 3,747,526 - Pf1N1B4_3361 0.45 -1.8 3,747,582 + +0.1 3,747,582 + Pf1N1B4_3361 0.48 -1.1 3,747,583 - Pf1N1B4_3361 0.48 -2.1 3,747,747 + Pf1N1B4_3361 0.55 -0.3 3,747,748 - Pf1N1B4_3361 0.55 -3.4 3,747,748 - Pf1N1B4_3361 0.55 +0.4 3,747,774 + Pf1N1B4_3361 0.57 +1.1 3,747,774 + Pf1N1B4_3361 0.57 -0.9 3,747,775 - Pf1N1B4_3361 0.57 +0.1 3,747,775 - Pf1N1B4_3361 0.57 -1.5 3,747,792 + Pf1N1B4_3361 0.58 -1.9 3,747,792 + +1.4 3,747,793 - Pf1N1B4_3361 0.58 -0.9 3,747,820 + Pf1N1B4_3361 0.59 -2.3 3,747,822 + Pf1N1B4_3361 0.59 -1.8 3,747,822 + Pf1N1B4_3361 0.59 -2.1 3,747,915 + Pf1N1B4_3361 0.63 -1.4 3,747,916 - Pf1N1B4_3361 0.63 -2.1 3,747,916 - Pf1N1B4_3361 0.63 -2.2 3,747,916 - Pf1N1B4_3361 0.63 -0.2 3,747,916 - Pf1N1B4_3361 0.63 -2.2 3,747,984 + Pf1N1B4_3361 0.67 -1.9 3,747,984 + Pf1N1B4_3361 0.67 -0.7 3,747,984 + -1.2 3,747,984 + Pf1N1B4_3361 0.67 -1.9 3,747,984 + Pf1N1B4_3361 0.67 -1.5 3,747,984 + Pf1N1B4_3361 0.67 -2.1 3,747,984 + Pf1N1B4_3361 0.67 -0.2 3,747,985 - Pf1N1B4_3361 0.67 +0.1 3,748,050 + Pf1N1B4_3361 0.70 -1.5 3,748,086 + Pf1N1B4_3361 0.71 -0.5 3,748,087 - Pf1N1B4_3361 0.72 -1.5 3,748,087 - Pf1N1B4_3361 0.72 -2.9 3,748,087 - Pf1N1B4_3361 0.72 +0.1 3,748,191 + Pf1N1B4_3361 0.76 -0.6 3,748,192 - -0.9 3,748,192 - Pf1N1B4_3361 0.77 -1.5 3,748,192 - Pf1N1B4_3361 0.77 -2.5 3,748,192 - Pf1N1B4_3361 0.77 -1.2 3,748,192 - -0.5 3,748,192 - +0.1 3,748,192 - Pf1N1B4_3361 0.77 +0.3 3,748,213 + Pf1N1B4_3361 0.78 +0.3 3,748,214 - Pf1N1B4_3361 0.78 -0.4 3,748,260 + Pf1N1B4_3361 0.80 +0.7 3,748,260 + Pf1N1B4_3361 0.80 -1.4 3,748,260 + Pf1N1B4_3361 0.80 +0.5 3,748,332 + Pf1N1B4_3361 0.83 -1.2 3,748,332 + Pf1N1B4_3361 0.83 -2.8 3,748,333 - Pf1N1B4_3361 0.83 -3.4 3,748,358 + Pf1N1B4_3361 0.84 -2.5 3,748,702 + -1.2 3,748,702 + -2.2 3,748,703 - -0.7 3,748,711 + -0.9 3,748,711 + -2.1 3,748,712 - +1.4 3,748,712 - -0.5 3,748,792 + -2.5 3,748,792 + -1.2 3,748,792 + -1.5 3,748,792 + -2.2 3,748,793 - -1.7 3,748,793 - -1.5 3,748,793 - -2.7 3,749,218 + -0.5 3,749,219 - Pf1N1B4_3362 0.43 +0.7 3,749,219 - Pf1N1B4_3362 0.43 -1.5 3,749,343 - Pf1N1B4_3362 0.53 -1.9 3,749,545 + Pf1N1B4_3362 0.70 -1.9 3,749,546 - Pf1N1B4_3362 0.70 -3.1 3,749,546 - Pf1N1B4_3362 0.70 -1.5 3,749,685 - Pf1N1B4_3362 0.81 -1.2
Or see this region's nucleotide sequence