Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2556

Experiment: Benzethonium chloride 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2554 and Pf1N1B4_2555 are separated by 82 nucleotidesPf1N1B4_2555 and Pf1N1B4_2556 are separated by 50 nucleotidesPf1N1B4_2556 and Pf1N1B4_2557 are separated by 70 nucleotides Pf1N1B4_2554 - Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17), at 2,897,002 to 2,897,340 _2554 Pf1N1B4_2555 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,897,423 to 2,898,223 _2555 Pf1N1B4_2556 - FIG00954311: hypothetical protein, at 2,898,274 to 2,899,251 _2556 Pf1N1B4_2557 - 2-nitropropane dioxygenase (EC 1.13.11.32), at 2,899,322 to 2,900,290 _2557 Position (kb) 2898 2899 2900Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.285 kb on + strand, within Pf1N1B4_2554at 2897.286 kb on - strand, within Pf1N1B4_2554at 2897.286 kb on - strand, within Pf1N1B4_2554at 2897.286 kb on - strand, within Pf1N1B4_2554at 2897.286 kb on - strand, within Pf1N1B4_2554at 2897.317 kb on - strandat 2897.317 kb on - strandat 2897.334 kb on + strandat 2897.377 kb on - strandat 2897.387 kb on + strandat 2897.436 kb on + strandat 2897.437 kb on - strandat 2897.437 kb on - strandat 2897.437 kb on - strandat 2897.437 kb on - strandat 2897.533 kb on + strand, within Pf1N1B4_2555at 2897.533 kb on + strandat 2897.534 kb on - strand, within Pf1N1B4_2555at 2897.534 kb on - strand, within Pf1N1B4_2555at 2897.568 kb on + strand, within Pf1N1B4_2555at 2897.568 kb on + strand, within Pf1N1B4_2555at 2897.568 kb on + strand, within Pf1N1B4_2555at 2897.568 kb on + strand, within Pf1N1B4_2555at 2897.568 kb on + strand, within Pf1N1B4_2555at 2897.569 kb on - strand, within Pf1N1B4_2555at 2897.569 kb on - strand, within Pf1N1B4_2555at 2897.569 kb on - strand, within Pf1N1B4_2555at 2897.569 kb on - strand, within Pf1N1B4_2555at 2897.569 kb on - strand, within Pf1N1B4_2555at 2897.739 kb on + strand, within Pf1N1B4_2555at 2897.739 kb on + strand, within Pf1N1B4_2555at 2897.739 kb on + strand, within Pf1N1B4_2555at 2897.739 kb on + strand, within Pf1N1B4_2555at 2897.740 kb on - strand, within Pf1N1B4_2555at 2897.740 kb on - strand, within Pf1N1B4_2555at 2897.755 kb on - strandat 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.757 kb on + strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2897.758 kb on - strand, within Pf1N1B4_2555at 2898.292 kb on + strandat 2898.292 kb on + strandat 2898.293 kb on - strandat 2898.293 kb on - strandat 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.401 kb on + strand, within Pf1N1B4_2556at 2898.402 kb on - strand, within Pf1N1B4_2556at 2898.402 kb on - strand, within Pf1N1B4_2556at 2898.402 kb on - strand, within Pf1N1B4_2556at 2898.402 kb on - strand, within Pf1N1B4_2556at 2898.525 kb on + strand, within Pf1N1B4_2556at 2898.583 kb on + strand, within Pf1N1B4_2556at 2898.584 kb on - strand, within Pf1N1B4_2556at 2898.586 kb on + strand, within Pf1N1B4_2556at 2898.586 kb on + strand, within Pf1N1B4_2556at 2898.586 kb on + strandat 2898.586 kb on + strand, within Pf1N1B4_2556at 2898.586 kb on + strand, within Pf1N1B4_2556at 2898.586 kb on + strand, within Pf1N1B4_2556at 2898.587 kb on - strand, within Pf1N1B4_2556at 2898.587 kb on - strand, within Pf1N1B4_2556at 2898.762 kb on + strand, within Pf1N1B4_2556at 2898.935 kb on + strand, within Pf1N1B4_2556at 2898.935 kb on + strand, within Pf1N1B4_2556at 2898.935 kb on + strand, within Pf1N1B4_2556at 2898.936 kb on - strand, within Pf1N1B4_2556at 2898.936 kb on - strand, within Pf1N1B4_2556at 2899.030 kb on + strand, within Pf1N1B4_2556at 2899.030 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.070 kb on + strand, within Pf1N1B4_2556at 2899.071 kb on - strandat 2899.071 kb on - strand, within Pf1N1B4_2556at 2899.071 kb on - strand, within Pf1N1B4_2556at 2899.071 kb on - strand, within Pf1N1B4_2556at 2899.282 kb on - strandat 2899.282 kb on - strandat 2899.284 kb on + strandat 2899.284 kb on + strandat 2899.284 kb on + strandat 2899.295 kb on - strandat 2899.309 kb on + strandat 2899.309 kb on + strandat 2899.309 kb on + strandat 2899.309 kb on + strandat 2899.310 kb on - strandat 2899.587 kb on - strand, within Pf1N1B4_2557at 2899.607 kb on - strand, within Pf1N1B4_2557at 2899.648 kb on + strand, within Pf1N1B4_2557at 2899.649 kb on - strand, within Pf1N1B4_2557at 2899.649 kb on - strand, within Pf1N1B4_2557at 2899.650 kb on + strand, within Pf1N1B4_2557at 2899.650 kb on + strand, within Pf1N1B4_2557at 2899.650 kb on + strand, within Pf1N1B4_2557at 2899.650 kb on + strand, within Pf1N1B4_2557at 2899.651 kb on - strand, within Pf1N1B4_2557at 2899.651 kb on - strand, within Pf1N1B4_2557at 2899.941 kb on + strand, within Pf1N1B4_2557at 2899.941 kb on + strand, within Pf1N1B4_2557at 2899.941 kb on + strand, within Pf1N1B4_2557at 2900.000 kb on + strand, within Pf1N1B4_2557at 2900.076 kb on + strand, within Pf1N1B4_2557at 2900.076 kb on + strandat 2900.180 kb on + strand, within Pf1N1B4_2557at 2900.224 kb on + strandat 2900.225 kb on - strandat 2900.243 kb on + strandat 2900.244 kb on - strandat 2900.244 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM
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2,897,285 + Pf1N1B4_2554 0.83 +1.7
2,897,285 + Pf1N1B4_2554 0.83 -0.8
2,897,285 + Pf1N1B4_2554 0.83 +0.8
2,897,285 + Pf1N1B4_2554 0.83 +0.6
2,897,285 + Pf1N1B4_2554 0.83 -0.2
2,897,285 + Pf1N1B4_2554 0.83 -1.0
2,897,285 + Pf1N1B4_2554 0.83 -2.0
2,897,286 - Pf1N1B4_2554 0.84 -0.7
2,897,286 - Pf1N1B4_2554 0.84 -1.2
2,897,286 - Pf1N1B4_2554 0.84 -0.6
2,897,286 - Pf1N1B4_2554 0.84 -2.4
2,897,317 - -0.0
2,897,317 - -1.0
2,897,334 + +1.8
2,897,377 - -1.3
2,897,387 + +3.0
2,897,436 + +0.2
2,897,437 - -0.4
2,897,437 - +0.8
2,897,437 - -0.2
2,897,437 - -0.1
2,897,533 + Pf1N1B4_2555 0.14 +0.2
2,897,533 + +1.4
2,897,534 - Pf1N1B4_2555 0.14 +0.4
2,897,534 - Pf1N1B4_2555 0.14 -1.2
2,897,568 + Pf1N1B4_2555 0.18 -1.2
2,897,568 + Pf1N1B4_2555 0.18 -1.0
2,897,568 + Pf1N1B4_2555 0.18 -2.0
2,897,568 + Pf1N1B4_2555 0.18 -1.0
2,897,568 + Pf1N1B4_2555 0.18 -0.1
2,897,569 - Pf1N1B4_2555 0.18 +0.8
2,897,569 - Pf1N1B4_2555 0.18 +0.0
2,897,569 - Pf1N1B4_2555 0.18 -1.6
2,897,569 - Pf1N1B4_2555 0.18 -0.1
2,897,569 - Pf1N1B4_2555 0.18 +0.8
2,897,739 + Pf1N1B4_2555 0.39 -2.1
2,897,739 + Pf1N1B4_2555 0.39 -1.4
2,897,739 + Pf1N1B4_2555 0.39 -1.8
2,897,739 + Pf1N1B4_2555 0.39 +0.3
2,897,740 - Pf1N1B4_2555 0.40 -1.0
2,897,740 - Pf1N1B4_2555 0.40 -1.0
2,897,755 - -0.2
2,897,757 + Pf1N1B4_2555 0.42 -0.6
2,897,757 + Pf1N1B4_2555 0.42 -0.6
2,897,757 + Pf1N1B4_2555 0.42 +0.8
2,897,757 + Pf1N1B4_2555 0.42 -0.5
2,897,757 + Pf1N1B4_2555 0.42 -2.7
2,897,757 + Pf1N1B4_2555 0.42 -0.9
2,897,758 - Pf1N1B4_2555 0.42 +0.6
2,897,758 - Pf1N1B4_2555 0.42 -1.4
2,897,758 - Pf1N1B4_2555 0.42 -1.4
2,897,758 - Pf1N1B4_2555 0.42 -1.5
2,897,758 - Pf1N1B4_2555 0.42 +0.8
2,897,758 - Pf1N1B4_2555 0.42 +0.4
2,897,758 - Pf1N1B4_2555 0.42 +0.0
2,897,758 - Pf1N1B4_2555 0.42 -0.5
2,897,758 - Pf1N1B4_2555 0.42 +0.4
2,897,758 - Pf1N1B4_2555 0.42 -0.2
2,897,758 - Pf1N1B4_2555 0.42 -0.7
2,898,292 + +0.4
2,898,292 + -0.6
2,898,293 - +2.4
2,898,293 - +0.1
2,898,401 + Pf1N1B4_2556 0.13 +1.6
2,898,401 + Pf1N1B4_2556 0.13 +0.8
2,898,401 + Pf1N1B4_2556 0.13 +1.9
2,898,401 + Pf1N1B4_2556 0.13 +0.0
2,898,401 + Pf1N1B4_2556 0.13 -0.2
2,898,401 + Pf1N1B4_2556 0.13 -0.5
2,898,401 + Pf1N1B4_2556 0.13 +0.0
2,898,402 - Pf1N1B4_2556 0.13 +0.3
2,898,402 - Pf1N1B4_2556 0.13 +2.8
2,898,402 - Pf1N1B4_2556 0.13 -1.2
2,898,402 - Pf1N1B4_2556 0.13 +0.6
2,898,525 + Pf1N1B4_2556 0.26 -0.1
2,898,583 + Pf1N1B4_2556 0.32 -0.2
2,898,584 - Pf1N1B4_2556 0.32 -0.4
2,898,586 + Pf1N1B4_2556 0.32 -3.2
2,898,586 + Pf1N1B4_2556 0.32 +0.4
2,898,586 + +1.9
2,898,586 + Pf1N1B4_2556 0.32 +1.9
2,898,586 + Pf1N1B4_2556 0.32 -1.6
2,898,586 + Pf1N1B4_2556 0.32 +0.7
2,898,587 - Pf1N1B4_2556 0.32 -0.3
2,898,587 - Pf1N1B4_2556 0.32 -0.1
2,898,762 + Pf1N1B4_2556 0.50 +1.8
2,898,935 + Pf1N1B4_2556 0.68 +0.6
2,898,935 + Pf1N1B4_2556 0.68 +1.0
2,898,935 + Pf1N1B4_2556 0.68 +0.9
2,898,936 - Pf1N1B4_2556 0.68 +0.2
2,898,936 - Pf1N1B4_2556 0.68 +1.4
2,899,030 + Pf1N1B4_2556 0.77 -0.9
2,899,030 + Pf1N1B4_2556 0.77 -1.1
2,899,070 + Pf1N1B4_2556 0.81 -0.3
2,899,070 + Pf1N1B4_2556 0.81 +0.4
2,899,070 + Pf1N1B4_2556 0.81 +0.9
2,899,070 + Pf1N1B4_2556 0.81 -2.2
2,899,070 + Pf1N1B4_2556 0.81 -1.2
2,899,070 + Pf1N1B4_2556 0.81 -0.0
2,899,071 - +2.4
2,899,071 - Pf1N1B4_2556 0.81 -0.2
2,899,071 - Pf1N1B4_2556 0.81 -1.6
2,899,071 - Pf1N1B4_2556 0.81 -1.0
2,899,282 - +0.6
2,899,282 - -2.2
2,899,284 + +0.4
2,899,284 + +1.1
2,899,284 + -1.4
2,899,295 - +0.0
2,899,309 + -1.1
2,899,309 + +1.1
2,899,309 + -1.2
2,899,309 + -1.8
2,899,310 - -2.5
2,899,587 - Pf1N1B4_2557 0.27 +0.1
2,899,607 - Pf1N1B4_2557 0.29 +2.5
2,899,648 + Pf1N1B4_2557 0.34 -3.0
2,899,649 - Pf1N1B4_2557 0.34 -1.6
2,899,649 - Pf1N1B4_2557 0.34 -1.6
2,899,650 + Pf1N1B4_2557 0.34 -0.5
2,899,650 + Pf1N1B4_2557 0.34 -1.6
2,899,650 + Pf1N1B4_2557 0.34 +0.3
2,899,650 + Pf1N1B4_2557 0.34 -2.2
2,899,651 - Pf1N1B4_2557 0.34 -1.0
2,899,651 - Pf1N1B4_2557 0.34 -0.6
2,899,941 + Pf1N1B4_2557 0.64 +1.0
2,899,941 + Pf1N1B4_2557 0.64 +1.6
2,899,941 + Pf1N1B4_2557 0.64 -1.8
2,900,000 + Pf1N1B4_2557 0.70 +1.5
2,900,076 + Pf1N1B4_2557 0.78 +0.6
2,900,076 + -0.2
2,900,180 + Pf1N1B4_2557 0.89 -2.3
2,900,224 + -0.2
2,900,225 - +1.4
2,900,243 + -2.3
2,900,244 - -1.0
2,900,244 - -1.2

Or see this region's nucleotide sequence