Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2321 and Pf1N1B4_2322 overlap by 8 nucleotides Pf1N1B4_2322 and Pf1N1B4_2323 are separated by 155 nucleotides Pf1N1B4_2323 and Pf1N1B4_2324 are separated by 60 nucleotides
Pf1N1B4_2321 - FIG015287: Zinc protease, at 2,640,800 to 2,642,290
_2321
Pf1N1B4_2322 - FIG015547: peptidase, M16 family, at 2,642,283 to 2,643,638
_2322
Pf1N1B4_2323 - hypothetical protein, at 2,643,794 to 2,643,922
_2323
Pf1N1B4_2324 - Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1), at 2,643,983 to 2,645,479
_2324
Position (kb)
2642
2643
2644 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2641.657 kb on - strand, within Pf1N1B4_2321 at 2642.060 kb on + strand, within Pf1N1B4_2321 at 2642.215 kb on - strand at 2642.310 kb on + strand at 2642.311 kb on - strand at 2642.311 kb on - strand at 2642.345 kb on - strand at 2642.345 kb on - strand at 2642.486 kb on - strand, within Pf1N1B4_2322 at 2642.514 kb on + strand, within Pf1N1B4_2322 at 2642.669 kb on - strand, within Pf1N1B4_2322 at 2643.017 kb on - strand, within Pf1N1B4_2322 at 2643.137 kb on - strand, within Pf1N1B4_2322 at 2643.467 kb on - strand, within Pf1N1B4_2322 at 2643.675 kb on - strand at 2643.928 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 2,641,657 - Pf1N1B4_2321 0.57 +1.6 2,642,060 + Pf1N1B4_2321 0.85 -0.9 2,642,215 - -1.2 2,642,310 + -0.2 2,642,311 - +0.6 2,642,311 - -1.9 2,642,345 - -0.9 2,642,345 - +1.4 2,642,486 - Pf1N1B4_2322 0.15 +1.4 2,642,514 + Pf1N1B4_2322 0.17 +1.0 2,642,669 - Pf1N1B4_2322 0.28 -1.2 2,643,017 - Pf1N1B4_2322 0.54 -1.2 2,643,137 - Pf1N1B4_2322 0.63 -1.6 2,643,467 - Pf1N1B4_2322 0.87 -3.1 2,643,675 - -0.7 2,643,928 + -3.1
Or see this region's nucleotide sequence