Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2170 and Pf1N1B4_2171 overlap by 4 nucleotides Pf1N1B4_2171 and Pf1N1B4_2172 are separated by 2 nucleotides Pf1N1B4_2172 and Pf1N1B4_2173 are separated by 13 nucleotides Pf1N1B4_2173 and Pf1N1B4_2174 are separated by 4 nucleotides
Pf1N1B4_2170 - Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase, at 2,483,621 to 2,484,322
_2170
Pf1N1B4_2171 - Tyrosine recombinase XerC, at 2,484,319 to 2,485,218
_2171
Pf1N1B4_2172 - Protein of unknown function DUF484, at 2,485,221 to 2,485,946
_2172
Pf1N1B4_2173 - Diaminopimelate epimerase (EC 5.1.1.7), at 2,485,960 to 2,486,790
_2173
Pf1N1B4_2174 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 2,486,795 to 2,488,042
_2174
Position (kb)
2485
2486 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2484.336 kb on + strand at 2484.368 kb on + strand at 2484.368 kb on + strand at 2484.368 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.405 kb on - strand at 2484.405 kb on - strand at 2484.405 kb on - strand at 2484.797 kb on + strand, within Pf1N1B4_2171 at 2484.798 kb on - strand, within Pf1N1B4_2171 at 2484.798 kb on - strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.977 kb on + strand, within Pf1N1B4_2171 at 2484.977 kb on + strand, within Pf1N1B4_2171 at 2484.978 kb on - strand, within Pf1N1B4_2171 at 2485.148 kb on + strand at 2485.227 kb on + strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.319 kb on - strand at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.626 kb on + strand, within Pf1N1B4_2172 at 2485.626 kb on + strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.918 kb on - strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.983 kb on - strand at 2485.983 kb on - strand at 2485.983 kb on - strand at 2486.071 kb on + strand, within Pf1N1B4_2173 at 2486.369 kb on + strand, within Pf1N1B4_2173 at 2486.369 kb on + strand, within Pf1N1B4_2173 at 2486.370 kb on - strand, within Pf1N1B4_2173 at 2486.370 kb on - strand, within Pf1N1B4_2173 at 2486.370 kb on - strand, within Pf1N1B4_2173 at 2486.370 kb on - strand, within Pf1N1B4_2173 at 2486.796 kb on + strand at 2486.797 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 2,484,336 + +1.5 2,484,368 + -1.1 2,484,368 + +0.3 2,484,368 + +0.2 2,484,404 + -0.8 2,484,404 + -1.3 2,484,404 + +1.5 2,484,404 + -0.8 2,484,404 + +0.8 2,484,405 - -0.8 2,484,405 - -1.8 2,484,405 - -1.5 2,484,797 + Pf1N1B4_2171 0.53 -0.0 2,484,798 - Pf1N1B4_2171 0.53 +3.5 2,484,798 - Pf1N1B4_2171 0.53 +0.3 2,484,967 + Pf1N1B4_2171 0.72 -0.8 2,484,967 + Pf1N1B4_2171 0.72 +0.5 2,484,967 + Pf1N1B4_2171 0.72 +0.8 2,484,967 + Pf1N1B4_2171 0.72 +2.1 2,484,977 + Pf1N1B4_2171 0.73 -0.7 2,484,977 + Pf1N1B4_2171 0.73 -0.5 2,484,978 - Pf1N1B4_2171 0.73 +0.4 2,485,148 + -0.8 2,485,227 + -1.1 2,485,286 - -0.5 2,485,286 - -0.1 2,485,286 - +1.0 2,485,286 - +1.2 2,485,286 - +0.5 2,485,318 + Pf1N1B4_2172 0.13 -1.5 2,485,318 + Pf1N1B4_2172 0.13 -1.7 2,485,318 + Pf1N1B4_2172 0.13 -1.7 2,485,318 + Pf1N1B4_2172 0.13 -1.1 2,485,319 - -0.5 2,485,319 - Pf1N1B4_2172 0.13 -0.6 2,485,319 - Pf1N1B4_2172 0.13 -0.1 2,485,319 - Pf1N1B4_2172 0.13 +1.1 2,485,626 + Pf1N1B4_2172 0.56 -0.1 2,485,626 + Pf1N1B4_2172 0.56 -0.5 2,485,627 - Pf1N1B4_2172 0.56 -1.8 2,485,627 - Pf1N1B4_2172 0.56 -1.1 2,485,627 - Pf1N1B4_2172 0.56 -1.9 2,485,821 + Pf1N1B4_2172 0.83 -1.9 2,485,821 + Pf1N1B4_2172 0.83 -2.4 2,485,821 + Pf1N1B4_2172 0.83 -1.3 2,485,822 - Pf1N1B4_2172 0.83 +0.3 2,485,822 - Pf1N1B4_2172 0.83 -0.1 2,485,822 - Pf1N1B4_2172 0.83 +1.5 2,485,918 - -1.9 2,485,982 + -0.5 2,485,982 + -1.1 2,485,982 + +0.9 2,485,982 + -1.3 2,485,983 - -2.0 2,485,983 - -1.5 2,485,983 - +1.3 2,486,071 + Pf1N1B4_2173 0.13 +1.8 2,486,369 + Pf1N1B4_2173 0.49 +0.5 2,486,369 + Pf1N1B4_2173 0.49 +0.5 2,486,370 - Pf1N1B4_2173 0.49 -0.8 2,486,370 - Pf1N1B4_2173 0.49 -1.7 2,486,370 - Pf1N1B4_2173 0.49 -2.0 2,486,370 - Pf1N1B4_2173 0.49 +0.5 2,486,796 + +0.5 2,486,797 - +0.5
Or see this region's nucleotide sequence