Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2100

Experiment: Benzethonium chloride 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2099 and Pf1N1B4_2100 are separated by 16 nucleotides Pf1N1B4_2099 - hypothetical protein, at 2,407,430 to 2,408,449 _2099 Pf1N1B4_2100 - EF hand domain protein, at 2,408,466 to 2,411,174 _2100 Position (kb) 2408 2409 2410 2411 2412Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2407.801 kb on - strand, within Pf1N1B4_2099at 2407.801 kb on - strand, within Pf1N1B4_2099at 2407.837 kb on - strand, within Pf1N1B4_2099at 2408.012 kb on + strand, within Pf1N1B4_2099at 2408.109 kb on + strand, within Pf1N1B4_2099at 2408.109 kb on + strand, within Pf1N1B4_2099at 2408.110 kb on - strand, within Pf1N1B4_2099at 2408.110 kb on - strand, within Pf1N1B4_2099at 2408.174 kb on + strand, within Pf1N1B4_2099at 2408.175 kb on - strand, within Pf1N1B4_2099at 2408.175 kb on - strand, within Pf1N1B4_2099at 2408.180 kb on + strand, within Pf1N1B4_2099at 2408.275 kb on + strand, within Pf1N1B4_2099at 2408.327 kb on - strand, within Pf1N1B4_2099at 2408.327 kb on - strand, within Pf1N1B4_2099at 2408.582 kb on + strandat 2408.583 kb on - strandat 2408.633 kb on + strandat 2408.634 kb on - strandat 2408.847 kb on - strand, within Pf1N1B4_2100at 2408.910 kb on - strand, within Pf1N1B4_2100at 2409.117 kb on - strand, within Pf1N1B4_2100at 2409.118 kb on + strand, within Pf1N1B4_2100at 2409.119 kb on - strand, within Pf1N1B4_2100at 2409.210 kb on + strand, within Pf1N1B4_2100at 2409.312 kb on - strand, within Pf1N1B4_2100at 2409.427 kb on + strand, within Pf1N1B4_2100at 2409.561 kb on - strand, within Pf1N1B4_2100at 2409.632 kb on + strand, within Pf1N1B4_2100at 2409.662 kb on + strand, within Pf1N1B4_2100at 2409.791 kb on + strand, within Pf1N1B4_2100at 2409.989 kb on - strand, within Pf1N1B4_2100at 2410.333 kb on + strand, within Pf1N1B4_2100at 2410.333 kb on + strand, within Pf1N1B4_2100at 2410.334 kb on - strand, within Pf1N1B4_2100at 2410.398 kb on - strand, within Pf1N1B4_2100at 2410.471 kb on + strand, within Pf1N1B4_2100at 2410.471 kb on + strand, within Pf1N1B4_2100at 2410.511 kb on - strand, within Pf1N1B4_2100at 2410.547 kb on + strand, within Pf1N1B4_2100at 2410.851 kb on + strand, within Pf1N1B4_2100at 2410.852 kb on - strand, within Pf1N1B4_2100at 2410.852 kb on - strand, within Pf1N1B4_2100at 2410.929 kb on - strandat 2410.949 kb on - strandat 2410.994 kb on - strandat 2411.094 kb on - strandat 2411.266 kb on - strandat 2411.307 kb on + strandat 2411.373 kb on + strandat 2411.377 kb on + strandat 2411.481 kb on + strandat 2411.579 kb on + strandat 2411.765 kb on + strandat 2411.791 kb on + strandat 2411.792 kb on - strandat 2411.797 kb on + strandat 2411.797 kb on + strandat 2411.873 kb on + strandat 2411.873 kb on + strandat 2411.873 kb on + strandat 2412.065 kb on + strandat 2412.075 kb on - strandat 2412.098 kb on + strandat 2412.124 kb on + strandat 2412.124 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM
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2,407,801 - Pf1N1B4_2099 0.36 +0.7
2,407,801 - Pf1N1B4_2099 0.36 -0.3
2,407,837 - Pf1N1B4_2099 0.40 -0.1
2,408,012 + Pf1N1B4_2099 0.57 +0.1
2,408,109 + Pf1N1B4_2099 0.67 +0.1
2,408,109 + Pf1N1B4_2099 0.67 -1.3
2,408,110 - Pf1N1B4_2099 0.67 +0.9
2,408,110 - Pf1N1B4_2099 0.67 -0.9
2,408,174 + Pf1N1B4_2099 0.73 -0.5
2,408,175 - Pf1N1B4_2099 0.73 -1.1
2,408,175 - Pf1N1B4_2099 0.73 -1.1
2,408,180 + Pf1N1B4_2099 0.74 -1.2
2,408,275 + Pf1N1B4_2099 0.83 -0.5
2,408,327 - Pf1N1B4_2099 0.88 -0.8
2,408,327 - Pf1N1B4_2099 0.88 -0.5
2,408,582 + -2.5
2,408,583 - -0.8
2,408,633 + -2.3
2,408,634 - +0.2
2,408,847 - Pf1N1B4_2100 0.14 -0.7
2,408,910 - Pf1N1B4_2100 0.16 -1.1
2,409,117 - Pf1N1B4_2100 0.24 -0.1
2,409,118 + Pf1N1B4_2100 0.24 -1.3
2,409,119 - Pf1N1B4_2100 0.24 -1.3
2,409,210 + Pf1N1B4_2100 0.27 +0.4
2,409,312 - Pf1N1B4_2100 0.31 -0.6
2,409,427 + Pf1N1B4_2100 0.35 -3.2
2,409,561 - Pf1N1B4_2100 0.40 +0.8
2,409,632 + Pf1N1B4_2100 0.43 -1.6
2,409,662 + Pf1N1B4_2100 0.44 -1.5
2,409,791 + Pf1N1B4_2100 0.49 -0.8
2,409,989 - Pf1N1B4_2100 0.56 -1.1
2,410,333 + Pf1N1B4_2100 0.69 -0.8
2,410,333 + Pf1N1B4_2100 0.69 +0.2
2,410,334 - Pf1N1B4_2100 0.69 -0.8
2,410,398 - Pf1N1B4_2100 0.71 -1.7
2,410,471 + Pf1N1B4_2100 0.74 -1.1
2,410,471 + Pf1N1B4_2100 0.74 +0.4
2,410,511 - Pf1N1B4_2100 0.75 -0.9
2,410,547 + Pf1N1B4_2100 0.77 -2.5
2,410,851 + Pf1N1B4_2100 0.88 -1.8
2,410,852 - Pf1N1B4_2100 0.88 -2.4
2,410,852 - Pf1N1B4_2100 0.88 -0.5
2,410,929 - +1.3
2,410,949 - +1.1
2,410,994 - -0.8
2,411,094 - -1.3
2,411,266 - +2.3
2,411,307 + +1.5
2,411,373 + +0.8
2,411,377 + -0.5
2,411,481 + +1.5
2,411,579 + +0.7
2,411,765 + -1.1
2,411,791 + -2.2
2,411,792 - +1.2
2,411,797 + +0.2
2,411,797 + -1.1
2,411,873 + -1.0
2,411,873 + -0.1
2,411,873 + -1.6
2,412,065 + +1.3
2,412,075 - -0.9
2,412,098 + -0.2
2,412,124 + -0.8
2,412,124 + +0.2

Or see this region's nucleotide sequence