Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2099 and Pf1N1B4_2100 are separated by 16 nucleotides
Pf1N1B4_2099 - hypothetical protein, at 2,407,430 to 2,408,449
_2099
Pf1N1B4_2100 - EF hand domain protein, at 2,408,466 to 2,411,174
_2100
Position (kb)
2408
2409
2410
2411
2412 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2407.801 kb on - strand, within Pf1N1B4_2099 at 2407.801 kb on - strand, within Pf1N1B4_2099 at 2407.837 kb on - strand, within Pf1N1B4_2099 at 2408.012 kb on + strand, within Pf1N1B4_2099 at 2408.109 kb on + strand, within Pf1N1B4_2099 at 2408.109 kb on + strand, within Pf1N1B4_2099 at 2408.110 kb on - strand, within Pf1N1B4_2099 at 2408.110 kb on - strand, within Pf1N1B4_2099 at 2408.174 kb on + strand, within Pf1N1B4_2099 at 2408.175 kb on - strand, within Pf1N1B4_2099 at 2408.175 kb on - strand, within Pf1N1B4_2099 at 2408.180 kb on + strand, within Pf1N1B4_2099 at 2408.275 kb on + strand, within Pf1N1B4_2099 at 2408.327 kb on - strand, within Pf1N1B4_2099 at 2408.327 kb on - strand, within Pf1N1B4_2099 at 2408.582 kb on + strand at 2408.583 kb on - strand at 2408.633 kb on + strand at 2408.634 kb on - strand at 2408.847 kb on - strand, within Pf1N1B4_2100 at 2408.910 kb on - strand, within Pf1N1B4_2100 at 2409.117 kb on - strand, within Pf1N1B4_2100 at 2409.118 kb on + strand, within Pf1N1B4_2100 at 2409.119 kb on - strand, within Pf1N1B4_2100 at 2409.210 kb on + strand, within Pf1N1B4_2100 at 2409.312 kb on - strand, within Pf1N1B4_2100 at 2409.427 kb on + strand, within Pf1N1B4_2100 at 2409.561 kb on - strand, within Pf1N1B4_2100 at 2409.632 kb on + strand, within Pf1N1B4_2100 at 2409.662 kb on + strand, within Pf1N1B4_2100 at 2409.791 kb on + strand, within Pf1N1B4_2100 at 2409.989 kb on - strand, within Pf1N1B4_2100 at 2410.333 kb on + strand, within Pf1N1B4_2100 at 2410.333 kb on + strand, within Pf1N1B4_2100 at 2410.334 kb on - strand, within Pf1N1B4_2100 at 2410.398 kb on - strand, within Pf1N1B4_2100 at 2410.471 kb on + strand, within Pf1N1B4_2100 at 2410.471 kb on + strand, within Pf1N1B4_2100 at 2410.511 kb on - strand, within Pf1N1B4_2100 at 2410.547 kb on + strand, within Pf1N1B4_2100 at 2410.851 kb on + strand, within Pf1N1B4_2100 at 2410.852 kb on - strand, within Pf1N1B4_2100 at 2410.852 kb on - strand, within Pf1N1B4_2100 at 2410.929 kb on - strand at 2410.949 kb on - strand at 2410.994 kb on - strand at 2411.094 kb on - strand at 2411.266 kb on - strand at 2411.307 kb on + strand at 2411.373 kb on + strand at 2411.377 kb on + strand at 2411.481 kb on + strand at 2411.579 kb on + strand at 2411.765 kb on + strand at 2411.791 kb on + strand at 2411.792 kb on - strand at 2411.797 kb on + strand at 2411.797 kb on + strand at 2411.873 kb on + strand at 2411.873 kb on + strand at 2411.873 kb on + strand at 2412.065 kb on + strand at 2412.075 kb on - strand at 2412.098 kb on + strand at 2412.124 kb on + strand at 2412.124 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 2,407,801 - Pf1N1B4_2099 0.36 +0.7 2,407,801 - Pf1N1B4_2099 0.36 -0.3 2,407,837 - Pf1N1B4_2099 0.40 -0.1 2,408,012 + Pf1N1B4_2099 0.57 +0.1 2,408,109 + Pf1N1B4_2099 0.67 +0.1 2,408,109 + Pf1N1B4_2099 0.67 -1.3 2,408,110 - Pf1N1B4_2099 0.67 +0.9 2,408,110 - Pf1N1B4_2099 0.67 -0.9 2,408,174 + Pf1N1B4_2099 0.73 -0.5 2,408,175 - Pf1N1B4_2099 0.73 -1.1 2,408,175 - Pf1N1B4_2099 0.73 -1.1 2,408,180 + Pf1N1B4_2099 0.74 -1.2 2,408,275 + Pf1N1B4_2099 0.83 -0.5 2,408,327 - Pf1N1B4_2099 0.88 -0.8 2,408,327 - Pf1N1B4_2099 0.88 -0.5 2,408,582 + -2.5 2,408,583 - -0.8 2,408,633 + -2.3 2,408,634 - +0.2 2,408,847 - Pf1N1B4_2100 0.14 -0.7 2,408,910 - Pf1N1B4_2100 0.16 -1.1 2,409,117 - Pf1N1B4_2100 0.24 -0.1 2,409,118 + Pf1N1B4_2100 0.24 -1.3 2,409,119 - Pf1N1B4_2100 0.24 -1.3 2,409,210 + Pf1N1B4_2100 0.27 +0.4 2,409,312 - Pf1N1B4_2100 0.31 -0.6 2,409,427 + Pf1N1B4_2100 0.35 -3.2 2,409,561 - Pf1N1B4_2100 0.40 +0.8 2,409,632 + Pf1N1B4_2100 0.43 -1.6 2,409,662 + Pf1N1B4_2100 0.44 -1.5 2,409,791 + Pf1N1B4_2100 0.49 -0.8 2,409,989 - Pf1N1B4_2100 0.56 -1.1 2,410,333 + Pf1N1B4_2100 0.69 -0.8 2,410,333 + Pf1N1B4_2100 0.69 +0.2 2,410,334 - Pf1N1B4_2100 0.69 -0.8 2,410,398 - Pf1N1B4_2100 0.71 -1.7 2,410,471 + Pf1N1B4_2100 0.74 -1.1 2,410,471 + Pf1N1B4_2100 0.74 +0.4 2,410,511 - Pf1N1B4_2100 0.75 -0.9 2,410,547 + Pf1N1B4_2100 0.77 -2.5 2,410,851 + Pf1N1B4_2100 0.88 -1.8 2,410,852 - Pf1N1B4_2100 0.88 -2.4 2,410,852 - Pf1N1B4_2100 0.88 -0.5 2,410,929 - +1.3 2,410,949 - +1.1 2,410,994 - -0.8 2,411,094 - -1.3 2,411,266 - +2.3 2,411,307 + +1.5 2,411,373 + +0.8 2,411,377 + -0.5 2,411,481 + +1.5 2,411,579 + +0.7 2,411,765 + -1.1 2,411,791 + -2.2 2,411,792 - +1.2 2,411,797 + +0.2 2,411,797 + -1.1 2,411,873 + -1.0 2,411,873 + -0.1 2,411,873 + -1.6 2,412,065 + +1.3 2,412,075 - -0.9 2,412,098 + -0.2 2,412,124 + -0.8 2,412,124 + +0.2
Or see this region's nucleotide sequence