Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2023 and Pf1N1B4_2024 are separated by 175 nucleotides Pf1N1B4_2024 and Pf1N1B4_2025 are separated by 538 nucleotides
Pf1N1B4_2023 - L-asparaginase (EC 3.5.1.1) (from data) , at 2,308,645 to 2,309,649
_2023
Pf1N1B4_2024 - sodium/alanine transporter, at 2,309,825 to 2,311,276
_2024
Pf1N1B4_2025 - Aspartate ammonia-lyase (EC 4.3.1.1), at 2,311,815 to 2,313,239
_2025
Position (kb)
2309
2310
2311
2312 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2308.931 kb on + strand, within Pf1N1B4_2023 at 2308.931 kb on + strand, within Pf1N1B4_2023 at 2309.037 kb on + strand, within Pf1N1B4_2023 at 2309.038 kb on - strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.048 kb on + strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.049 kb on - strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.177 kb on + strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.178 kb on - strand, within Pf1N1B4_2023 at 2309.339 kb on + strand, within Pf1N1B4_2023 at 2309.340 kb on - strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.522 kb on + strand, within Pf1N1B4_2023 at 2309.523 kb on - strand, within Pf1N1B4_2023 at 2309.523 kb on - strand, within Pf1N1B4_2023 at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.604 kb on - strand at 2309.753 kb on + strand at 2309.754 kb on - strand at 2310.126 kb on - strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.198 kb on + strand, within Pf1N1B4_2024 at 2310.199 kb on - strand at 2310.199 kb on - strand, within Pf1N1B4_2024 at 2310.199 kb on - strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.201 kb on + strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.202 kb on - strand, within Pf1N1B4_2024 at 2310.207 kb on + strand, within Pf1N1B4_2024 at 2310.207 kb on + strand, within Pf1N1B4_2024 at 2310.208 kb on - strand, within Pf1N1B4_2024 at 2310.333 kb on + strand, within Pf1N1B4_2024 at 2310.333 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.624 kb on + strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.625 kb on - strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.642 kb on + strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.643 kb on - strand, within Pf1N1B4_2024 at 2310.662 kb on + strand, within Pf1N1B4_2024 at 2310.747 kb on + strand, within Pf1N1B4_2024 at 2310.747 kb on + strand, within Pf1N1B4_2024 at 2310.747 kb on + strand, within Pf1N1B4_2024 at 2310.748 kb on - strand, within Pf1N1B4_2024 at 2310.891 kb on + strand, within Pf1N1B4_2024 at 2310.892 kb on - strand, within Pf1N1B4_2024 at 2311.206 kb on + strand at 2311.206 kb on + strand at 2311.206 kb on + strand at 2311.207 kb on - strand at 2311.239 kb on - strand at 2311.281 kb on + strand at 2311.335 kb on - strand at 2311.402 kb on + strand at 2311.402 kb on + strand at 2311.402 kb on + strand at 2311.403 kb on - strand at 2311.403 kb on - strand at 2311.403 kb on - strand at 2311.403 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.406 kb on - strand at 2311.471 kb on - strand at 2311.471 kb on - strand at 2311.471 kb on - strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.472 kb on + strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.473 kb on - strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.476 kb on + strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.477 kb on - strand at 2311.560 kb on + strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.561 kb on - strand at 2311.564 kb on + strand at 2311.564 kb on + strand at 2311.564 kb on + strand at 2311.564 kb on + strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.565 kb on - strand at 2311.734 kb on - strand at 2311.734 kb on - strand at 2311.734 kb on - strand at 2311.734 kb on - strand at 2311.767 kb on + strand at 2311.767 kb on + strand at 2311.768 kb on - strand at 2311.768 kb on - strand at 2311.982 kb on - strand, within Pf1N1B4_2025
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 2,308,931 + Pf1N1B4_2023 0.28 -1.4 2,308,931 + Pf1N1B4_2023 0.28 +0.2 2,309,037 + Pf1N1B4_2023 0.39 +0.2 2,309,038 - Pf1N1B4_2023 0.39 +1.0 2,309,048 + Pf1N1B4_2023 0.40 -0.6 2,309,048 + Pf1N1B4_2023 0.40 +1.9 2,309,048 + Pf1N1B4_2023 0.40 +0.7 2,309,049 - Pf1N1B4_2023 0.40 +1.6 2,309,049 - Pf1N1B4_2023 0.40 -0.4 2,309,049 - Pf1N1B4_2023 0.40 -0.0 2,309,049 - Pf1N1B4_2023 0.40 -2.4 2,309,177 + Pf1N1B4_2023 0.53 -1.7 2,309,177 + Pf1N1B4_2023 0.53 +1.6 2,309,177 + Pf1N1B4_2023 0.53 -1.0 2,309,178 - Pf1N1B4_2023 0.53 +0.2 2,309,178 - Pf1N1B4_2023 0.53 +2.0 2,309,178 - Pf1N1B4_2023 0.53 +0.2 2,309,178 - Pf1N1B4_2023 0.53 +0.8 2,309,178 - Pf1N1B4_2023 0.53 +1.2 2,309,178 - Pf1N1B4_2023 0.53 +1.3 2,309,339 + Pf1N1B4_2023 0.69 -0.0 2,309,340 - Pf1N1B4_2023 0.69 +0.7 2,309,522 + Pf1N1B4_2023 0.87 -1.2 2,309,522 + Pf1N1B4_2023 0.87 +0.8 2,309,522 + Pf1N1B4_2023 0.87 +1.0 2,309,523 - Pf1N1B4_2023 0.87 +0.6 2,309,523 - Pf1N1B4_2023 0.87 +1.5 2,309,604 - +2.0 2,309,604 - +1.0 2,309,604 - +1.6 2,309,604 - -1.7 2,309,604 - -1.4 2,309,604 - +0.2 2,309,753 + -0.1 2,309,754 - -1.2 2,310,126 - Pf1N1B4_2024 0.21 -1.7 2,310,198 + Pf1N1B4_2024 0.26 +1.6 2,310,198 + Pf1N1B4_2024 0.26 +0.6 2,310,198 + Pf1N1B4_2024 0.26 -1.0 2,310,199 - +2.0 2,310,199 - Pf1N1B4_2024 0.26 -0.0 2,310,199 - Pf1N1B4_2024 0.26 +3.2 2,310,201 + Pf1N1B4_2024 0.26 +0.7 2,310,201 + Pf1N1B4_2024 0.26 -0.9 2,310,201 + Pf1N1B4_2024 0.26 -1.2 2,310,201 + Pf1N1B4_2024 0.26 -0.9 2,310,201 + Pf1N1B4_2024 0.26 +0.6 2,310,201 + Pf1N1B4_2024 0.26 -1.0 2,310,202 - Pf1N1B4_2024 0.26 -0.7 2,310,202 - Pf1N1B4_2024 0.26 +1.5 2,310,202 - Pf1N1B4_2024 0.26 -0.0 2,310,202 - Pf1N1B4_2024 0.26 -2.7 2,310,202 - Pf1N1B4_2024 0.26 -0.1 2,310,202 - Pf1N1B4_2024 0.26 -0.6 2,310,202 - Pf1N1B4_2024 0.26 +0.9 2,310,202 - Pf1N1B4_2024 0.26 -0.0 2,310,202 - Pf1N1B4_2024 0.26 -0.0 2,310,207 + Pf1N1B4_2024 0.26 -0.4 2,310,207 + Pf1N1B4_2024 0.26 -1.2 2,310,208 - Pf1N1B4_2024 0.26 +0.1 2,310,333 + Pf1N1B4_2024 0.35 -0.1 2,310,333 + Pf1N1B4_2024 0.35 +2.4 2,310,624 + Pf1N1B4_2024 0.55 +0.3 2,310,624 + Pf1N1B4_2024 0.55 +0.8 2,310,624 + Pf1N1B4_2024 0.55 +1.3 2,310,624 + Pf1N1B4_2024 0.55 -0.8 2,310,624 + Pf1N1B4_2024 0.55 -0.2 2,310,625 - Pf1N1B4_2024 0.55 -0.0 2,310,625 - Pf1N1B4_2024 0.55 -0.7 2,310,625 - Pf1N1B4_2024 0.55 -0.0 2,310,625 - Pf1N1B4_2024 0.55 -0.4 2,310,625 - Pf1N1B4_2024 0.55 -1.9 2,310,625 - Pf1N1B4_2024 0.55 +1.2 2,310,625 - Pf1N1B4_2024 0.55 -2.4 2,310,642 + Pf1N1B4_2024 0.56 -0.3 2,310,642 + Pf1N1B4_2024 0.56 -1.4 2,310,642 + Pf1N1B4_2024 0.56 +1.0 2,310,642 + Pf1N1B4_2024 0.56 -0.4 2,310,642 + Pf1N1B4_2024 0.56 +0.3 2,310,642 + Pf1N1B4_2024 0.56 +1.1 2,310,643 - Pf1N1B4_2024 0.56 -1.0 2,310,643 - Pf1N1B4_2024 0.56 -0.1 2,310,643 - Pf1N1B4_2024 0.56 -0.7 2,310,643 - Pf1N1B4_2024 0.56 -0.7 2,310,662 + Pf1N1B4_2024 0.58 -1.7 2,310,747 + Pf1N1B4_2024 0.63 +0.3 2,310,747 + Pf1N1B4_2024 0.63 -1.9 2,310,747 + Pf1N1B4_2024 0.63 -1.6 2,310,748 - Pf1N1B4_2024 0.64 -0.0 2,310,891 + Pf1N1B4_2024 0.73 -0.0 2,310,892 - Pf1N1B4_2024 0.73 -0.7 2,311,206 + -1.0 2,311,206 + -0.6 2,311,206 + -0.4 2,311,207 - +0.6 2,311,239 - +0.6 2,311,281 + -0.2 2,311,335 - +0.7 2,311,402 + -0.6 2,311,402 + -2.3 2,311,402 + +1.6 2,311,403 - +1.3 2,311,403 - +1.3 2,311,403 - +0.2 2,311,403 - +1.1 2,311,406 - +1.2 2,311,406 - +3.6 2,311,406 - +1.2 2,311,406 - +1.8 2,311,406 - +1.9 2,311,406 - +1.9 2,311,406 - +0.9 2,311,406 - +1.9 2,311,406 - +0.8 2,311,406 - +1.0 2,311,471 - +0.7 2,311,471 - +1.2 2,311,471 - -0.7 2,311,472 + -0.4 2,311,472 + +0.6 2,311,472 + -1.7 2,311,472 + -1.0 2,311,472 + +0.4 2,311,472 + +0.6 2,311,472 + -2.6 2,311,472 + -1.6 2,311,472 + -0.7 2,311,472 + +1.6 2,311,472 + +1.6 2,311,472 + -0.5 2,311,472 + -0.0 2,311,472 + -0.4 2,311,472 + -1.0 2,311,472 + -1.6 2,311,472 + -1.7 2,311,472 + -1.6 2,311,472 + +1.3 2,311,473 - +0.6 2,311,473 - +1.7 2,311,473 - +0.5 2,311,473 - +1.4 2,311,473 - +0.2 2,311,473 - +0.7 2,311,473 - +1.6 2,311,473 - +1.9 2,311,473 - -0.2 2,311,473 - +0.3 2,311,473 - +0.8 2,311,473 - +0.4 2,311,473 - +2.2 2,311,473 - +1.9 2,311,473 - +0.4 2,311,473 - +0.7 2,311,473 - +0.6 2,311,473 - +0.8 2,311,473 - -0.4 2,311,473 - +1.7 2,311,473 - +0.3 2,311,473 - +0.8 2,311,473 - +0.8 2,311,473 - +1.2 2,311,473 - +3.2 2,311,473 - -0.0 2,311,473 - +1.2 2,311,473 - +0.9 2,311,473 - +0.7 2,311,473 - +0.9 2,311,473 - +1.2 2,311,473 - +0.6 2,311,473 - +0.2 2,311,473 - +1.2 2,311,473 - +1.1 2,311,473 - +1.3 2,311,473 - -0.6 2,311,473 - +1.5 2,311,473 - +0.7 2,311,473 - +1.3 2,311,473 - +1.6 2,311,473 - +1.8 2,311,473 - +0.9 2,311,476 + -1.6 2,311,476 + -0.4 2,311,476 + +1.6 2,311,476 + -1.4 2,311,476 + +0.8 2,311,476 + +2.2 2,311,476 + -0.7 2,311,476 + -0.6 2,311,476 + -0.7 2,311,476 + +1.4 2,311,477 - +1.7 2,311,477 - +1.9 2,311,477 - +1.5 2,311,477 - +1.3 2,311,477 - +0.7 2,311,477 - +1.6 2,311,477 - +1.2 2,311,477 - +0.7 2,311,477 - +1.5 2,311,477 - +1.3 2,311,477 - +1.1 2,311,477 - +1.1 2,311,477 - +0.9 2,311,477 - +0.4 2,311,477 - +0.9 2,311,477 - +1.3 2,311,477 - +0.2 2,311,477 - +0.2 2,311,477 - +0.5 2,311,477 - +1.2 2,311,477 - +2.2 2,311,477 - +1.0 2,311,477 - +1.4 2,311,477 - -0.5 2,311,560 + +1.6 2,311,561 - +1.3 2,311,561 - +2.1 2,311,561 - -0.6 2,311,561 - +1.0 2,311,561 - +1.1 2,311,561 - +1.1 2,311,561 - -0.0 2,311,561 - +2.9 2,311,561 - +0.3 2,311,561 - +0.9 2,311,561 - +0.5 2,311,561 - +0.9 2,311,564 + +1.8 2,311,564 + +1.6 2,311,564 + -0.4 2,311,564 + +2.3 2,311,565 - +0.5 2,311,565 - -0.0 2,311,565 - -0.2 2,311,565 - +2.8 2,311,565 - +2.1 2,311,565 - +0.7 2,311,565 - +1.6 2,311,565 - -1.0 2,311,565 - +0.3 2,311,734 - +0.4 2,311,734 - +0.8 2,311,734 - +1.3 2,311,734 - -0.4 2,311,767 + -0.0 2,311,767 + +0.8 2,311,768 - +0.5 2,311,768 - -0.0 2,311,982 - Pf1N1B4_2025 0.12 +3.2
Or see this region's nucleotide sequence